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Isolation of Nitrogen-Fixing Bacteria from Gramineous Crops and Measurement of Nitrogenase Activity  

최은화 (경북대학교 미생물학과)
이상은 (경북대학교 미생물학과)
윤기순 (경북대학교 생물공학과)
권덕기 (경북대학교 생물공학과)
손재근 (경북대학교 농학과)
박승환 (한국생명공학연구원 유전체연구센터)
한명숙 (경북대학교 미생물학과)
김사열 (경북대학교 미생물학과)
Publication Information
Microbiology and Biotechnology Letters / v.31, no.1, 2003 , pp. 18-24 More about this Journal
Abstract
For researching nitrogen-fixing bacteria associated with gramineous crops, we collected growing roots of rices, wheats, oats, barleys, ryes, and maizes at 19 sites of southern Korean peninsula. Endophytes and free living bacteria were isolated from those crop roots. Sixty-three isolates were classified on the basis of different morphology, size, color, host of colony, and the 16S rDNAs sequence. The analyses of PCR amplification for nifH gene and nitrogenase activity assay, revealed that all isolates contained nitrogen-fixing abilities. In addition, most of them have cellulase activity which is one of the common features of endophytic bacteria from plant.
Keywords
Nitrogen-fixing bacteria; 16S rDNA sequence; nitrogenase; cellulase;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By SCOPUS : 8
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