• 한국수산과학회지
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• 제50권4호
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• pp.406-412
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• 2017

Vocalizations of the common dolphin Delphinus Delphis, were recorded from a single hydrophone while coastal visual survey was performed in the East Sea in March 2017. Like most Delphinidae, common dolphins produce whistles, echolocation clicks, and burst-pulsed calls, with repertoires that differ between species and geographically separated populations. This study focuses on using frequency analysis to classify sounds into three categories and to compare them with those of other common dolphin populations. The fundamental frequencies of the whistles were high in the 6 to 14 kHz range, while echolocation clicks were in the 40 to 90 kHz frequency band. These results are similar to high-energy distributions in the same frequency band in other regions. Based on this study of the acoustic characteristics of dolphins off the east coast of Korea, cetacean vocalizations can be classified using a database of cetacean sounds in Korea. In addition, this technique could improve data quality for visual whale surveys and could be applied to various other research subjects.

• 수산해양기술연구
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• 제55권1호
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• pp.39-49
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• 2019

Acoustics are increasingly regarded as a remote-sensing tool that provides the basis for classifying and mapping ocean resources including seabed classification. It has long been understood that details about the character of the seabed (roughness, sediment type, grain-size distribution, porosity, and material density) are embedded in the acoustical echoes from the seabed. This study developed a sophisticated yet easy-to-use technique to discriminate seabed characteristics using a split beam echosounder. Acoustic survey was conducted in Tongyeong waters, South Korea in June 2018, and the verification of acoustic seabed classification was made by the Van Veen grab sampler. The acoustic scattering signals extracted the seabed hardness and roughness components as well as various seabed features. The seabed features were selected using the principal component analysis, and the seabed classification was performed by the K-means clustering. As a result, three seabed types such as sand, mud, and shell were discriminated. This preliminary study presented feasible application of a sounder to classify the seabed substrates. It can be further developed for characterizing marine habitats on a variety of spatial scales and studying the ecological characteristic of fishes near the habitats.

• 수산해양기술연구
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• 제55권2호
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• pp.129-137
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• 2019

This study was conducted to investigate the effects of underwater noise caused by pile driving during marine construction on fish. In this study, the three gray rockfish were released about 1 km away from the construction site of wind power generation on July 18, 2018 and traced using two acoustic telemetry techniques. The behavior of the fish was analyzed by calculating the moving distance, swimming speed and direction of the gray rockfish. In the results of the acoustic tracking using the ship, the rockfish moved about 2.11 km for about two hours at a speed of $0.28{\pm}0.14m/s$ (0.94 TL/s). The bottom depth of the trajectory of the rockfish was $1.0{\pm}0.6m$ on average. There was a significant directionality in swimming direction of the gray rockfish, and there was no significant correlation between the swimming direction and tidal current direction. Moving distance during 5 minutes (5MD) during pile driving and finishing operations between rock surface and bedrock were 0.94-0.96 times (76.0-77.0 m) and 1.81-2.73 times (146.0-219.5 m), respectively, compared with no pile driving. This study is expected to be used as a basic data of fish behavior research on underwater noise.

• 수산해양기술연구
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• 제58권1호
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• pp.39-48
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• 2022

Using environmental DNA (eDNA) in the fisheries and oceanography fields, research on the diversity of biological species, the presence or absence of specific species and quantitative evaluation of species has considerably been performed. Up to date, no study on eDNA has been tried in the area of fisheries acoustics in Korea. In this study, the biomass of a dominant species in the northwestern waters of Jeju Island was examined using 1) the catch ratio of the species from trawl survey results and 2) the ranking ratio of the species from the eDNA results. The dominant species was Zoarces gillii, and its trawl catch ratio was 68.2% and its eDNA ratio was 81.3%. The Zoarces gillii biomass from the two methods was 7199.4 tons (trawl) and 8584.6 tons (eDNA), respectively. The mean and standard deviation of the acoustic backscattering strength values (120 kHz) from the entire survey area were 135.5 and 157.7 m²/nm², respectively. The strongest echo signal occurred at latitude 34° and longitude 126°15' (northwest of Jeju Island). High echo signals were observed in a specific oceanographic feature (salinity range of 32-33 psu and the water temperature range of 19-20℃). This study was a pilot study on evaluating quantitatively aquatic resources by applying the eDNA technique into acoustic-trawl survey method. Points to be considered for high-quality quantitative estimation using the eDNA to fisheries acosutics were discussed.

• The Journal of the Acoustical Society of Korea
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• 제25권3E호
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• pp.101-109
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• 2006

Besides dorsal-aspect target strength (TS) of the fish, side-aspect TS information is also acoustically important parameter in fisheries acoustics. In this study, the side-aspect TS of 11 black sea bream (Acanthopagurus schlegeli) were measured using a split beam echosounder of 120, 200, and 420 kHz; total length of the fish ranged from 12.4 to 23.7 cm, and wet weight from 27.5 to 229.8g. For the precise TS measurement with side-aspect angle, we used anesthetized and tethered specimens of known size while being rotated through $360^{\circ}C$ by means of a carousel structure. The side-aspect TS measurements of the fish were conducted by rotating the fish in the horizontal plane at 50 interval. The ping interval was 0.2 second and elapsed time at each angle was 30-60 second. As a result, the measured side-aspect TS data were fitted by sinusoidal function. The relationships between fish length and near full side-aspect TS were as follows: $TS_{120kHz}= 21.46 log (TL)-67.5 (r = 0.70), \;TS_{200 kHz}= 31.03 log (TL)-76.9 (r=0.83),\;TS_{420 kHz}= 30.79 log (TL)-72.2 (r = 0.77)$. For comparison, theoretically estimated side-aspect TS from the Kirchhoff ray mode (KRM) model, which based on swimbladder and body morphology, were compared with the measured TS.

• Ocean and Polar Research
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• 제30권1호
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• pp.79-87
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• 2008

Hydroacoustic technique was used to analyze spatiotemporal stability and distribution of demersal fish aggregations in the coastal region to overcome some limitations of the existing methods such as net and diving. The survey was carried out in the Baekeum Bay on the south coast of Korea in January 2007. The bottom depth in the study site ranges from 7 to 25 m. In order to outline aggregations of demersal fish initial scanning using 200 kHz split-beam transducer was randomly conducted over the large area. Having detected fish aggregation in the specific region, intensive acoustic survey of irregular star pattern was carried out along 14 transects across the area in question. The results of the acoustic survey show that all demersal fish aggregations are concentrated about 5 m from sea bottom having a slight slope and remain steady with no spatial or temporal variations during acoustic survey. The hydroacoustic method used in this study offers a new approach to understand vertical and horizontal distribution, spatiotemporal stability, and biomass estimate of demersal fish aggregations in coastal regions. Additionally, the number of individual fish estimated from in situ acoustic target strength data can be used to understand the standing stock of demersal fish aggregation.

• 수산해양기술연구
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• 제38권4호
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• pp.271-277
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• 2002

고래류의 어업공해를 최소화하고 환경친화적으로 이용할 수 있는 음향 유인/경고시스템을 개발하기 위한 기초연구의 일환으로 우리 나라 연근해에 자주 출현하는 큰돌고래의 휘슬음을 서울대공원 돌고래 쇼장에서 측정, 분석한 결과를 요약하면 다음과 같다. 1 수족관 내에서 생활하고 있는 큰돌고래의 평상시 휘슬음의 중심 주파수대와 스팩트럼 레벨은 각각 6～10KHz 와 85㏈을 나타내었고, 이들을 서로 격리시킨 경우, 중심 주파수는 6.7KHz대와 21.3KHz대 두 개의 고조파를 나타내었으며, 스팩트럼 레벨은 각각 110㏈와 94㏈를 나타내어 평상시보다는 불안정한 휘슬음의 주파수대와 다른 형태를 나타내었다. 2. 평상시의 휘슬음 주파수 변동폭은 평균 3.86KHz이었고, 지속시간은 평균 0.08sec를 나타내었다. 그러나 격리시킨 경우는 평상시와는 달리 그 변동폭은 평균 l4.06KHz이었고, 지속시간은 평균 0.l9sec를 나타내어 평상시와 비교하여 주파수 변동폭이 10.20KHz 높아지고 지속시간은 0.11sec 길어짐을 알 수 있었다. 더욱이 Mann-Whitney 검정을 통하여 휘슬음의 주파수 변동폭과 지속시간 모두 평상시와 1마리를 격리시켰을 때는 상이함에 유의성을 확인할 수 있었다. 3. 돌고래 쇼장에서의 휘슬음의 패턴 모델을 6가지 형태로 분류할 수 있었고, 평상시에는 5～10KHz 범위의 주파수로 서서히 상승하는 형이 거의 대부분이었으나, 격리시킨 경우에는 5～20KHz의 폭 넓은 주파수대에 걸쳐 여러 가지 패턴을 관찰할 수 있었으며, 특히 주파수가 상승한 후 일정하게 유지되는 형태의 패턴이 많이 관찰되었다. 한편, 휘슬음은 종간 및 생활환경에 따라 사용되는 주파수 대역폭이 서로 다른 것을 예상할 수 있으므로 우리 나라 연안에는 큰돌고래, 참돌고래, 낫돌고래, 상괭이 등 비교적 많은 돌고래류가 자주 출현하고 있어 이들 종들에 대한 위협음 및 유인음에 대한 대역을 파악하기 위해서는 각 종들에 대한 수중음향은 물론 행동도 함께 분석할 필요가 있으며 더욱이 폭 넓은 현장실험과 데이터 축적을 병행하여야 할 것이다.

• 한국음향학회지
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• 제36권3호
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• pp.172-178
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• 2017

초음파는 세포사멸을 포함하여 의학 및 생물학분야에 널리 응용되고 있으나 그 정확한 기작에 대해선 논쟁의 여지가 있다. 본 연구에서는 40 kH 초음파 조사시스템을 단세포 효모에 적합하게 개발하고 세포사멸 유도시 40 kH 초음파의 생물학적 현상을 살펴보았다. 아이오딘화 칼륨 선량 측정법을 이용하여 1.5 ml 실험튜브에 40 kH 초음파 조사 시스템의 최적 조건을 맞추어 세포사멸을 시간 의존적 방식으로 연구하였고 초음파 조사과정동안 온열효과와는 별개로 세포 사멸이 관찰되었다. 40 kH 초음파와 과산화수소의 동시 처리는 세포사멸에 상조적인 효과가 관찰되어 활성산소가 40 kH 초음파사멸에 관련이 있었다. 그러나 활성산소 저해제, NAC(N-acetyl-Lcysteine)는 초음파에 의한 세포사멸에 약한 영향만을 미쳤고 다른 세포사멸, 괴사억제제[글리실리진(glycyrrhizin) 또는 zVAD-fmk] 역시도 세포사멸을 완전히 억제하진 못하였다. 본 연구를 통하여 40 kH 초음파에 의한 세포사멸에는 온열효과나 활성산소만으로 사멸이 유도되지는 않는 것으로 보인다.