• Fisheries and Aquatic Sciences
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• v.17 no.3
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• pp.319-324
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• 2014

An 8 weeks feeding trial was carried out to evaluate the effects of dietary microbial phytase (P) supplementation on nutrient digestibility, and body composition in juvenile olive flounder Paralichthys olivaceus fed soybean meal-based diets. Seven experimental diets were formulated to be isonitrogenous and isocaloric to contain 50.0% crude protein (CP) and 16.7 kJ of available energy/g with or without dietary phytase supplementation. White fish meal (FM) provided 92.4% of the total protein in the basal diet ($S_0$), in the other 6 diets, 30% or 40% FM protein was replaced by soybean meal: 70% FM + 30% soybean meal ($S_{30}$); 70% FM + 30% SM + 1000 U phytase/kg diet ($S_{30}P_{1000}$); 70% FM + 30% SM + 2000 U phytase/kg diet ($S_{30}P_{2000}$); 60% FM + 40% SM ($S_{40}$); 60% FM + 40% SM + 1000 U phytase/kg diet ($S_{40}P_{1000}$); and 60% FM + 40% SM + 2000 U phytase/kg diet ($S_{40}P_{2000}$). After two weeks of the conditioning period, triplicate groups of 25 fish initially averaging $6.15{\pm}0.04g$ ($mean{\pm}S.D.$) were randomly distributed into the aquarium and were fed one of the experimental diets for 8 weeks. After feeding trial, supplementation of phytase significantly improved the apparent digestibility coefficients of phosphorus in flounder diets (P<0.05) containing 30% and 40% soybean meal regardless the levels. However, phytase had no significant influence on growth performance and whole body composition of fish. Based on the experimental results, we conclude that dietary supplementation of phytase could improve the apparent digestibility coefficient of phosphorus in olive flounder.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.4
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• pp.443-448
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• 2022

Two long-term feeding trials were conducted to compare the effects on survival and growth of juvenile sea cucumber Apostichopus japonicus of experimental diets containing corn distillers dried grains with solubles, Squid Sepioteuthis sepioidea liver meal, and brown fish meal with a commercial die. In both first and second trials, two experimental diets and one commercial diet were assessed. After a 45-week feeding period in trial 1, survival did not differ significantly among groups. The final mean weight of sea cucumbers fed either experimental diet was significantly higher than that of sea cucumbers fed the commercial diet. After a 15-week feeding period in trial 2, survival and final mean weight of sea cucumbers fed either experimental practical diets was significantly higher than that of sea cucumbers fed the commercial diet. It was concluded that the experimental diets formulations can be used as a practical diet, and inclusion of corn distillers dried grains with solubles, squid liver meal, and brown fish meal can improve the growth of juvenile sea cucumbers.

• Journal of Aquaculture
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• v.11 no.1
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• pp.39-48
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• 1998

Growth performance and excretions of nitrogen (N) and phosphorus (P) were investigated with carp (Cyprinus carpio) with carp (Cyprinus carpio) grower fed a low pollution diets (A and B) and commercial ones (Com-1 and Com-2). A recirculated rearing system (Exp. I) and a floating net cage system (Exp. II) were employed for two feeding trials in which fish having an initial body weight of 152g and 193g were fed for 41 an 39 days, respectively. The highest weight gain, daily growth rate and protein efficiency ratio were found (P<0.05) in fish fed diet A containing 10% fish meal and 2% monocalcium phosphate (MCP) for both experiments. They also showed the lowest feed conversion ratio and daily feeding ration among treatments. Fish fed diet B containing 10% fish meal, 5% fish protein concentrate and 1% MCP showed weight gain and FCR more improved (P<0.05) than those fed commercial diets. In all groups, whole body cmpositions were not greatly different among treatment, and protein and P contents in final fish ranged from 14.3 to 15.6% and from 0.39 to 0.48%, respectively. Fish fed diet A excreted the least N which were 38.3 in Exp. I and 39.6g/kg gain in Exp. II. However, the values found in fish fed two commercial diets amounted to 59.1 and 58.9g, respectively. A significant decrease in P excretion was also found in fish groups fed diets A and B. In Exp. I, a reduction of 53.4% was shown in fish fed diet A, compared to the averaged value (18.5g P/kg gain) of two commercial groups. In Exp. II conducted using the floating net cages, fish fed diet B excreted the least P (8.6g/kg gain) among the treatment, which was followed by fish fed diet A, showing 48.6% reduction compared to the average value (18.5g/kg gain) for fish fed two commercial diets. The present results clearly showed that N and P excretions from fish culture could be significantly reduced by using the low pollution diet.

• Journal of Aquaculture
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• v.10 no.1
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• pp.77-85
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• 1997

A 6-week feeding trial was conducted to determine the nutritional value of various dietary animal protein sources in juvenile Koran rockfish. White fish meal (WFM), flounder muscle meal (FMM), blood meal (BM), casein & gelain (CG), egg white albumin (EWA) and squid liver powder (SLP) were used as the animal protein sources, Crude protein content and available energy of the experimental diets were 50% and 15.9 kJ/g, respectively. There were significant differences among all dietary groups in weight gain (WG), feed efficiency (FE), and protein effiency ratio (PER). WFM and FFM were the best animal protein sources among the dietary groups. FMM diet had significantly higher (P<0.05) WG, FE, and PER values than those of fish fed the WFM diet. WG, FE, PER, and specific growth rate (SGR) values of fish fed BM diet were significantly higher (P<0.05) than those of fish fed EWA diet. Significant differences were found in whole body composition, hemoglobin (Hb), hepatosomatic index (HSI), and hematocrit (Ht). These results showed that low-temperature processing of lyophilized flounder muscle meal resulted in superior performance of rockfish relative to the other evaluated animal protein sources.

• Journal of Aquaculture
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• v.1 no.2
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• pp.109-119
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• 1988

Following the previous study on the nutritional quantity of Al, Fe, and their interrelationships with other trace metals, this study was conducted to determine the effect of supplementation of Cu and Mn to fish meal-diets on the growth of Japanese eel. The feeding experiment to determine Cu requirement was conducted using white fish meal-diets supplemented with 0, 5, 10, or 20 $\mu$g/g cupric sulfate, and that to determine Mn requirement was conducted by suppling the diet with 0, 10, 20, or 40 $\mu$g/g manganese sulfate. The results revealed that the fish on diet annexed with 5 $\mu$g/g of Cu were observed to have the best growth among these groups. In case of Mn, 20 $\mu$g/g of supplementary level promoted growth rate and 40 $\mu$g/g of diet resulted in the highest feed efficiency. On the other hand, the highest level of Cu (20 $\mu$g/g), and Mn (40 $\mu$g/g) supplementary diets did not expressed adverse effect on growth. These results indicated that the suitable Cu and Mn supplementary concentrations are 20 $\mu$g/g and 30 $\mu$g/g of formulated the white fish meal-diet respectively. The white fish meal had 1.6 $\mu$g/g of Cu and 6.1 $\mu$g/g of Mn.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.4
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• pp.314-319
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• 2010

This study determined the proper inclusion levels of squid liver powder (SLP) - soy protein concentrate (SPC) premix as a fish meal (FM) substitute in the diet for juvenile olive flounder (Paralichthys olivaceus). All the experimental diets were formulated to be isonitrogenous (50% crude protein) and isocaloric (17.1 kJ energy $g^{-1}$ diet). Five diets were formulated with SLP-(SPC) replacing 0, 25, 50, 75, and 100% of the FM (SLP $(SPC)_0$, SLP $(SPC)_25$, SLP $(SPC)_50$, SLP $(SPC)_75$, and SLP $(SPC)_100$, respectively). Triplicate groups of 12 fish averaging $5.56{\pm}0.10\;g$ ($mean{\pm}SD$) were fed one of the five experimental diets for 7 weeks. The weight gain (WG), specific growth rate (SGR), feed efficiency (FE), and protein efficiency ratio (PER) of fish fed SLP $(SPC)_0$ were significantly (P<0.05) higher than those of fish fed SLP $(SPC)_50$, SLP $(SPC)_75$ and SLP $(SPC)_100$, while those of fish fed SLP $(SPC)_0$ and SLP $(SPC)_25$ were similar. The whole-body crude protein content of fish fed SLP $(SPC)_0$ was significantly (P<0.05) lower than that of fish fed SLP $(SPC)_50$, SLP $(SPC)_75$, and SLP$(SPC)_100$, while those of fish fed SLP $(SPC)_0$ and SLP $(SPC)_25$ were similar. The whole-body crude lipid of fish fed SLP $(SPC)_0$ was significantly (P<0.05) lower than that of fish fed SLP $(SPC)_50$ and SLP $(SPC)_75$, while those of fish fed SLP $(SPC)_0$, SLP $(SPC)_25$ and SLP $(SPC)_100$ were similar. Based on the fish growth performance results obtained from this study, the SLP (SPC) premix can substitute up to 25% of the FM in the diets of juvenile olive flounder.

• Korean Journal of Community Nutrition
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• v.20 no.1
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• pp.21-29
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• 2015

Objectives: Maintaining a balanced diet is very crucial for adolescents. However, adolescents, who may have a short notion about the amount of food, find it difficult to plan daily meals by applying the target pattern proposed by the Korean Nutrition Society. This study was carried out to revise the target pattern based on cooked dishes instead of raw material food groups as an easier way for Korean adolescents to plan their meals. Methods: Target pattern for Korean adolescents were revised based on the following: $1^{st}$, categorize dish groups, $2^{nd}$, calculate representative values of each dish based on the adolescent' intake amount. $3^{rd}$, assign the recommended number of intake for each dish. Validity of the target pattern for Korean adolescent meal plan was examined by the energy content, energy contribution ratio, and NAR & INQ of nutrients. Results: The 11 dish groups categorized were bab; gook tang gigae; side dishes of meat, fish, egg, legume, kimchi, vegetable, seaweed; and between meal of fruit, and milk dairy product. Based on the representative energy values, recommended number of intake were assigned to each dish. For boys, bab and gook tang gigae: 3 each; meat, fish, egg, and legume: 1 each; kimchi and vegetable: 3 each; seaweed: 1; fruit and milk dairy product: 2 each were assigned. For girls, bab and gook tang gigae: 2 each; meat, fish, egg, and legume: choice of 3 dishes, 1 each; kimchi and vegetable: 3 each; seaweed: 1; fruit and milk dairy product: 2 each were assigned. Energy contents, energy contribution ratio of carbohydrate, protein, and fat for boys and girls were in adequate range. The NARs were 1.0 and INQs were ${\geq}1.0$ for all nutrients examined. Conclusions: Revised dish-based, instead of food-based adolescent target patterns for boys and girls were easier and a valid way of Korean adolescent meal planning.

• Journal of Animal Science and Technology
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• v.65 no.5
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• pp.1002-1013
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• 2023

The objectives of the present study were to determine the nutrient digestibility of fish meal, defatted black soldier fly larvae (BSFL), and adult flies and to develop equations for estimating in vitro nutrient digestibility of BSFL for pigs. In vitro digestion procedures were employed to mimic the digestion and absorption of nutrients in the pig intestine. Correlation coefficients between chemical composition and in vitro nutrient digestibility of BSFL were calculated. In Exp. 1, in vitro ileal digestibility (IVID) of dry matter (DM) and crude protein (CP) and in vitro total tract digestibility (IVTTD) of DM and organic matter in defatted BSFL meal were less (p < 0.05) than those in fish meal but were greater (p < 0.05) than those in adult flies. In Exp. 2, CP concentrations in BSFL were negatively correlated with ether extract (r = -0.91) concentration but positively correlated with acid detergent fiber (ADF; r = 0.98) and chitin (r = 0.95) concentrations. ADF and chitin concentrations in BSFL were negatively correlated with IVID of DM (r = -0.98 and -0.88) and IVTTD of DM (r = -1.00 and -0.94) and organic matter (r = -0.99 and -0.98). Prediction equations for in vitro nutrient digestibility of BSFL were developed: IVID of CP (%) = -0.95 × ADF (% DM) + 95 (r² = 0.75 and p = 0.058) and IVTTD of DM (%) = -2.09 × ADF + 113 (r² = 0.99 and p < 0.001). The present in vitro experiments suggest that defatted BSFL meal was less digestible than fish meal but was more digestible than adult flies, and nutrient digestibility of BSFL can be predicted using ADF as an independent variable.

• Journal of Fisheries and Marine Sciences Education
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• v.29 no.3
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• pp.888-898
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• 2017

Tenebrio molitor larvae, also known as yellow mealworms (MW), are rich in protein and lipid and can serve as a potential alternative protein and energy source in commercial aquafeeds. Therefore, this study attempts to evaluate the effects of different drying methods on the nutritional value of MW meal. For this, live MW were cold-anaesthetized before being subjected to three different types of drying methods, including freeze-drying, oven-drying at $60^{\circ}C$ and air-drying at room temperature for three days, and compared for proximate composition and energy content. An in-vivo digestibility test was then conducted to evaluate the nutrient digestibility of MW meal in diets for rockfish, Sebastes schlegeli. A test diet was prepared by mixing the MW meal with a reference diet (Ref) in a 30:70 ratio with chromium oxide as an inert marker at the inclusion level of 0.5%. Rockfish with mean body weight of 150 g were stocked into a fecal collection system equipped with fiberglass tanks of 400 L capacity. Each group of fish was fed one of the experimental diets to apparent satiation for 4 weeks. The results of the proximate analysis showed that drying methods had no significant effect on crude protein, crude lipid, ash and energy contents of MW. Despite being a rich source of protein and lipid, MW meal was deficient in certain amino acids, particularly methionine, and highly unsaturated fatty acids, particularly 22:6n-3 (DHA) and 20:5n-3 (EPA). MW meal showed high digestibility values for protein (93%), lipid (97%) and energy (88%). These results may indicate that MW meal is a nutritious and acceptable feed ingredient, with comparable digestibility values to conventional animal and plant feedstuffs such as fish meal and soybean meal, in practical diet for rockfish at grower stage.

• Journal of Fisheries and Marine Sciences Education
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• v.27 no.2
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• pp.390-398
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• 2015

A feeding experiment was conducted to determine the use of distillers dried grain (DDG) as a partial replacement for fish meal in the diet for juvenile rockfish, Sebastes schlegeli. Four iso-nitrogenous (50% crude protein) and iso-caloric (4.3 kcal/g) diets (designated as DDG0, DDG7, DDG14, and DDG21) were formulated to contain 0, 7, 14, and 21% DDG. Triplicate groups of juvenile rockfish (initial body weight, $10.2{\pm}0.2g$) were fed one of the experimental diets to visual satiety twice a day (09:00 and 17:00) for 8 weeks. At the end of the feeding trial, survival of rockfish was above 97% and not affected by dietary DDG levels (P>0.05). Weight gain, feed efficiency and daily feed intake of juvenile rockfish were significantly decreased with increase of dietary DDG levels (P<0.05). Condition factor, hepatosomatic index and visceralsomatic index of juvenile rockfish were not significantly affected by dietary DDG levels (P>0.05). No significant differences were observed in the contents of moisture, crude protein, crude lipid and ash of the whole body and dorsal muscle in juvenile rockfish fed the experimental diets (P>0.05). Therefore dietary inclusion of DDG as a replacement for fish meal could depress the growth of juvenile rockfish.