• 한국어병학회지
• /
• 제25권3호
• /
• pp.165-172
• /
• 2012

Vibrio alginolyticus (V. alginolyticus) 는 우리나라 전 연안에서 높은 빈도로 발견되며 인간 및 어패류에 감염을 유발시키는 박테리아의 일종이다. 본 연구는 서해안 양식장의 해수와 어패류에서 V. alginolyticus와 이에 대한 특이적인 용균성 파아지를 분리하였으며 분리된 파아지의 형태학적 특성을 전자현미경으로 확인 하였다. 또한 파아지의 핵산의 종류 및 구조 단백질의 특성 대한 연구가 수행되었다. 분리된 파아지는 형태학적으로 60 nm의 육각형 두부와 20 nm의 짧은 미부를 가진 podoviridae과로 분류되었다. 핵산을 분리한 결과 23 Kb 크기의 DNA로 판명 되었으며 구조 단백질은 37.8 kDa과 198 kDa 사이에 7종류의 단백질 분획이 존재함을 확인 하였다.

• 생태와환경
• /
• 제49권2호
• /
• pp.130-141
• /
• 2016

This study was conducted to elucidate the structure of river ecosystem in the watershed of the Seom River and Hoengseong Reservoir located in Hoengseong of Kangwon Province from February to October 2007. Topics of the survey were mainly rainfall, discharge, water quality in abiotic factors and attached algae, benthic macroinvertebrates, fish, birds and mammals of flora and fauna in a biotic factors, respectively. Specifically, the Seom River could be seen as a typical flow rate of the stream is controlled to the effect of the dam. Basic water qualities were great to seasonal effects, it was relatively clean. Diatom Achnanthes, Cymbella, Gomphonema, and Navicula were distributed predominantly in the periphytic algae. Benthic macroinvertebrates were mostly aquatic insects and freshwater shellfish, the aquatic insects were abundant Ephemeroptera and Trichoptera. Freshwater fish was the dominant Zacco platypus, fish species varied toward the downstream. Birds were mainly observed in four species, and species Egretta garzetta, which was distributed in a wide area of the Seom River. In mammals, Lutra lutra of Mustelidae was identified that the number of inhabit widely. In aspects of the ecological trophic level, the Seom River was maintained at a relatively stable state in the producer and the consumer relationship. The results of this study will expected to be utilized as a useful data for understanding the structure and function of the lotic and lentic ecosystems.

• 한국식품영양과학회지
• /
• 제2권1호
• /
• pp.49-54
• /
• 1973

Ever-Fresh(주성분(主成分) : kusayate) 처리(處理)가 붕장어, 보리새우, 학꽁치, 빙장중(氷藏中)의 선도유지(鮮度維持)에 미치는 효과(效果)를 실험(實驗)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. K치(値)는 붕장어, 학꽁치 및 보리새우의 사후(死後) 초기(初期)의 신선도(新鮮度)를 판정(判定)하는 좋은 지표(指標)가 될 수 있다. 2. 보리새우의 사후(死後) 초기(初期)의 선도판정(鮮度判定)에는 VB-N도 좋은 지표(指標)가 될 수 있다. 3. Ever-Fresh 처리(處理)는 붕장어, 학꽁치, 보리새우의 빙장(氷藏) 초기(初期)의 선도유지(鮮度維持)에 효과(效果)가 있었다. 4. Ever-Fresh 처리(處理)는 보리새우 빙장(氷藏) 초기(初期)의 산화방지(酸化防止) 효과(效果)가 있었다.

• 한국식품과학회지
• /
• 제52권1호
• /
• pp.89-93
• /
• 2020

본 연구에서는 패혈증비브리오균에 대한 PEM, RCM, RRM과 CTM 메탄올 추출물의 항균 활성을 평가하였다. 96-well microplates를 사용한 액체 배양법 실험 결과 4가지 천연물은 200 ㎍/mL 농도에서 80%이상의 항균 효과를 나타내었다. 또한 페이퍼디스크 확산법을 사용하여 항균 활성을 평가한 결과 4가지 천연물은 20, 40 mg/disc의 농도에서 농도 의존적으로 패혈증비브리오균의 성장을 억제하였다. 다음으로 4가지 천연물의 안전성을 평가하기 위해 MTS assay를 통해 HeLa 세포에 대한 독성을 확인한 결과 100, 200 ㎍/mL의 농도에서 세포 독성을 나타내지 않았다. 또한 LDH assay를 사용하여 패혈증비브리오균의 HeLa 세포에 대한 세포막 손상 억제 효과를 평가한 결과 PEM, RCM, RRM과 CTM 메탄올 추출물은 200 ㎍/mL 농도에서 세포막 손상억제 효과를 나타내었다. 다음으로 바닷물과 조개를 사용한 수족관 모델에서 4가지 천연물의 메탄올 추출물 혼합액 200 ㎍/mL 농도는 colony 생성을 현저하게 감소시켜 패혈증비브리오균의 증식을 억제 하는 결과를 나타내었다. 이와 같은 결과를 통해 4가지 천연물의 메탄올 추출물은 바닷물과 해산물에 존재하는 패혈증비브리오균에 대해 뛰어난 항균효과를 나타내면서 인체에는 무해한 천연소독제로 응용할 수 있을 것으로 판단된다.

• 한국식생활문화학회지
• /
• 제37권1호
• /
• pp.26-38
• /
• 2022

This study used the big data method to analyze the chronological frequency of seafood appearance and variety mentioned by the veritable records of the Joseon dynasty. The findings will be used as a basis for Joseon Period's food cultural research. The web-crawling method was used to digitally scrap from the veritable records of the Joseon dynasty of Joseon's first to the twenty-seventh king. A total of 9,536 cases indicated the appearance of seafood out of the 384,582 articles. Seafood were termed "seafood" as a collective noun 107 times (1.12%), 27 types of fish 8,372 times (87.79%), 3 types of mollusca (1.28%), 18 types of shellfish 213 times (2.23%), 6 types of crustacean 188 times (1.97%), 9 types of seaweed 534 times (5.60%). Fish appeared most frequently out of all the recorded seafood. Sea fish appeared more frequently than the freshwater fish. Kings that showed the most Strong Interest Inventory (SII) were: Sungjong from the 15^thcentury, Sehjo from the 15^th, Youngjo from the 18^th, Sehjong from the 15^th, and Jungjo from the 18^th respectively. Kings of Chosen were most interested in seafood in the 15^th and 18^th centuries.

• 한국식생활문화학회지
• /
• 제7권4호
• /
• pp.339-362
• /
• 1992

The Guk was divided Malgunjangguk, Tojangguk, and Gooumguk as to cooking method. Gooumguk was boiled with several materials of beef, bone, organs and water for the long time, and a good source of protein, calcium, and phosphorus. In the thesis, according to the kinds of Gooumguk, the reference frequency to them, the adding foods in them, and the variety cooking method in the Gooumguk were analyzed by the cooking books published from 1670 to 1988 in Korea. 1. There were 41 kinds of Gooumguk and Kaejang, Joktang and Byultang were firstly published to Omshikdimibang in 1670. 2. Many kinds of Gooumguk were generally used on royal parties in Yi dynasty and were used by several substances. 3. The main substances of Gooumguk were meat, poultry, fish, and shellfish. The seasoning substances were green onion, garlic, ginger and black pepper etc, and were used for the removal of the meat's or fish's off-flavor. 4. Yangtang, Kalbitang, Joktang, Yeongebacksuk, and Chooatang etc expressed the taste of the one main substance and Japtang, Sulnongtang, Yukgejang, Yongbongtang etc were combinated the taste of the several materials. 5. Cooking methods of Yukgaejang and Koritang before 19 century were different in present.: Yukgaejang was not used Gochoojang or powdered red pepper and Koritang was used Gochoojang.

• Journal of Microbiology and Biotechnology
• /
• 제30권10호
• /
• pp.1443-1457
• /
• 2020

The emergence and spread of antimicrobial resistance in pathogenic bacteria of fish and shellfish have caused serious concerns in the aquaculture industry, owing to the potential health risks to humans and animals. Among these bacteria, Aeromonas salmonicida, which is one of the most important primary pathogens in salmonids, is responsible for significant economic losses in the global aquaculture industry, especially in salmonid farming because of its severe infectivity and acquisition of antimicrobial resistance. Therefore, interest in the use of alternative approaches to prevent and control A. salmonicida infections has increased in recent years, and several applications of bacteriophages (phages) have provided promising results. For several decades, A. salmonicida and phages infecting this fish pathogen have been thoroughly investigated in various research areas including aquaculture. The general overview of phage usage to control bacterial diseases in aquaculture, including the general advantages of this strategy, has been clearly described in previous reviews. Therefore, this review specifically focuses on providing insights into the phages infecting A. salmonicida, from basic research to biotechnological application in aquaculture, as well as recent advances in the study of A. salmonicida.

• 종양간호연구
• /
• 제12권4호
• /
• pp.331-338
• /
• 2012

Purpose: This descriptive study aimed to compare the perception of the acceptable foods for the neutropenic diet between nurses and patients by food type. Methods: The participants were 225 nurses working at chemotherapy wards and 71 patients in chemotherapy treatment. Data were collected with a self-administered questionnaire from January 2 to February 24, 2012, and analyzed with SPSS 12.0 program using descriptive statistics and the ${\chi}^2$-test. Results: Eighty-eight point eight percent of nurses and 76.1% of cancer patients thought the patients needed the neutropenic diets. The most important decisional criteria to determine dietary restriction was neutrophil count for nurses and food type for patients. The two groups showed significantly different allowance to raw fruits and raw vegetables, sterilized canned juice, fried vegetables, yogurt, uncooked tofu, salted fish, cooked fish, cooked shellfish, uncooked grain powder, jellified food, home-made bread/cookies, nuts including peanuts, instant coffee or tea and tea brewed. In general, patients were more permissive about the neutropenic diet than nurses. Conclusion: It is recommended to consider patients' preference as well as nurses' professional knowledge and publish standardized clinical diet guidelines for neutropenic patients with collaboration between nurses and patient representatives.

• 한국식품조리과학회지
• /
• 제6권3호통권12호
• /
• pp.67-83
• /
• 1990

Currentry, the originality of traditional soups in Kyoung-Nam province is vanishing by the inflex of processed and instant food, food industrial development. Especially, such factors as the influence of alien cooked, the develoment of transportation, the movement of population have made it hard to preserve the traditionnal food preparations. The purpose of this thesis was to seek out the various kinds of Kyoung-Nam province soups preparation. To do this, about 60 kinds of soups recipe were reviewed and charactered into several categories. The findings of the study are as follows. 1) Soup is made by fish or shellfish, freshwater fish. Meat and fowls is rarely used in Kyoung-Nam province (coastal region). 2) Soups of inland area is made by most vegetables and these soup were always harmonized with soybean powder or perilla powder. 3) Various soup season with the mag-jang, mul-jang sauce, hab-ja-jang sauce. 4) These soups were in general to make much red pepper and much salt.

• 한국어병학회지
• /
• 제27권2호
• /
• pp.107-114
• /
• 2014

세균성 어류질병의 치료 및 예방을 위하여 어류와 인체에 안전한 수산용 probiotic의 개발이 요구되고 있다. 본 연구에서는 남해안 일대에서 양식되고 있는 참굴 (Crassostrea gigas), 바지락 (Ruditapes philippinarum), 피조개 (Scapharca broughtonii), 새조개 (Fulvia mutica)의 가식 부위로부터 17균주의 candidate probiotic bacteria (CPB)를 분리하였다. 나아가 다양한 연쇄상구균 (Lactococcus garvieae, L. piscium, Streptococcus sp., S. iniae, and S. parauberis)에 대하여 강한 생장 억제력을 보이는 균주(CPB-St)를 선별하여 어류의 연쇄구균증 관리를 위한 probiotic 균주로서의 개발 가능성을 알아보았다. CPB-St 균주를 double layer test를 통하여 다양한 연쇄상구균들에 대한 생장 억제 정도를 알아보았으며, 혼합 배양에서의 Streptococcus sp.에 대한 생장 억제 능력을 확인하였고, CPB-St의 어류에 대한 안전성 및 장내 생존 유무를 평가하였다. CPB-St는 대부분의 연쇄상구균에 대하여 18~26 mm의 생장 억제대를 형성함으로써 높은 생장 억제 능력을 보였다. CPB-St와의 혼합 배양에서 Streptococcus sp.는 6시간째부터 생장 억제 현상이 관찰되기 시작하여 12시간 전후로 8~55배 정도의 감소를 나타내었다. CPB-St의 어류에 대한 병원성 유 무를 알아본 결과, 2주일 동안 CPB-St로 인한 어류 폐사는 관찰되지 않았다. CPB-St의 1회 강제 경구 투여 후, 위와 장에서 CPB-St의 생존을 확인한 결과 투여 24시간 후에는 CPB-St가 모두 배출되는 것으로 나타났다. CPB-St를 probiotic bacteria로 개발하기 위해서는 어류의 장내 정착 가능성과 먹이를 통한 효과의 정확한 검증이 추후 이루어져야 할 것이다.