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http://dx.doi.org/10.9721/KJFST.2020.52.1.89

Antibacterial effects of natural products on Vibrio vulnificus growth in seawater and shellfish  

Hong, Yeongjin (Department of Microbiology, Chonnam National University Medical School)
Ryu, Phil Youl (Department of Microbiology, Chonnam National University Medical School)
Kim, Seung Yong (College of Pharmacy, Chonnam National University)
Yeom, Kyung Min (College of Pharmacy, Chonnam National University)
Park, Jung Up (College of Pharmacy, Chonnam National University)
Jo, Se Jin (College of Pharmacy, Chonnam National University)
Guo, Rui Hong (College of Pharmacy, Chonnam National University)
Tran, The Bach (Institute of Ecology and Biological Resources (IEBR), Vietnam Academy of Science and Technology (VAST))
Jin, Hang (Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences (YAAS))
Kim, Young Ran (College of Pharmacy, Chonnam National University)
Publication Information
Korean Journal of Food Science and Technology / v.52, no.1, 2020 , pp. 89-93 More about this Journal
Abstract
Vibrio vulnificus, an opportunistic pathogen, causes septicaemia when raw shellfish and fish are eaten by patients with hepatic diseases or reduced immunity. In this study, we evaluated inhibitory effects of some natural products on V. vulnificus growth using 96-well microplate assay. We found that Phyllanthus emblica L., Rosa chinensis Jacq., Rosa rugose Thub., and Chukrasia tabularis A. Juss. significantly inhibited V. vulnificus growth in Luria-Bertani (LB) broth. Among these four extracts, the inhibition diameter of Chukrasia tabularis was 16.00 ± 0.58 mm in disc diffusion assay on V. vulnificus growth. In addition, these four natural products protected HeLa cells from V. vulnificus-induced cytotoxicity. A cocktail containing these four products showed an inhibitory effect on V. vulnificus growth in seawater and shellfish by reducing its growth by 75.7% and 97%, respectively. These results suggest that these four natural products are safe and effective natural antimicrobial candidates to prevent V. vulnificus infection.
Keywords
Vibrio vulnificus; antibacterial activity; shellfish; natural products;
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Times Cited By KSCI : 2  (Citation Analysis)
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