• Mycobiology
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• 제44권3호
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• pp.187-190
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• 2016

In July 2015, diseased leaves of black chokeberry (Aronia melanocarpa) were observed in Danyang and Gochang, Korea. The symptoms appeared as circular or irregular brown leaf spots, from which Alternaria tenuissima was isolated. The isolates were cultured on potato dextrose agar, and their morphological characteristics were observed under a light microscope. The colonies were whitish to ash colored. The pathogenicity test on healthy black chokeberry leaves produced circular brown spots, in line with the original symptoms. Molecular analyses of the ITS, GPD, RPB2, and TEF genes were conducted to confirm the identity of the pathogen. The phylogeny of the multi-gene sequences indicated that the causal agent was A. tenuissima. This study is the first report of A. tenuissima leaf spot on black chokeberry (A. melanocarpa).

• Natural Product Sciences
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• 제20권4호
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• pp.301-305
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• 2014

The aim of our study is to investigate the anti-nociceptive and anti-inflammatory properties of an ethanol extract of the leaf and stem of Aralia cordata. Writhing responses induced by acetic acid, tail immersion test, and formalin-induced paw pain response for nociception and formalin-induced paw edema for inflammation were evaluated in mice. A. cordata (50 - 200 mg/kg, p.o.) and ibuprofen (100 mg/kg, p.o.), a positive non-steroidal anti-inflammatory drugs (NSAIDs), inhibited the acetic acid-induced writhing response, but they did not protect the thermal nociception in tail immersion test. However, morphine (5 mg/kg, s.c.) used as positive opioid control alleviated both the acetic acid-induced writhing response and thermal nociception in tail immersion test. In the formalin test, A. cordata (50 - 200mg/kg) and ibuprofen (200mg/kg) inhibited the second phase response (peripheral inflammatory response), but not the first phase response (central response), whereas morphine inhibited both phase pain responses. Both A. cordata (100 mg/kg) and ibuprofen (200 mg/kg) significantly alleviated the formalin-induced increase of paw thickness, the index of inflammation. These results show for the first time that the leaf and stem of A. cordata has a significant anti-nociceptive effect that seems to be peripheral, but not central. A. cordata also displays an anti-inflammatory activity in an acute inflammation model. The present study supports a possible use of the leaf and stem of A. cordata to treat pain and inflammation.

• The Plant Pathology Journal
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• 제19권4호
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• pp.200-202
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• 2003

Leaf spots were first observed on watermelon (Citrullus vulgaris Schrad) under polyethylene film-covered green-house in November 2002. Symptoms appeared as dark-brown circles or large irregular spots on the leaves of watermelon. Occasionally, zonal growth of the lesions was observed. Under humid conditions, small black sclerotium-like bodies (sporodochia) were produced on the surface of the lesions. The sporodochia on leaf lesions were sessile, polymorphic, variable in size, 35-850 $\mu\textrm{m}$ in diameter, and 30-470 $\mu\textrm{m}$ in depth. Conidia in sporodochium were black in mass, one-celled, rod-shaped, with rounded ends, hyaline, guttulate, and measured 6-8$\times$1.6-2.2 $\mu\textrm{m}$ in size. The pathogen was identified as Myrothecium roridum Tode ex Fr. This is the first report of Myrothecium leaf spot on watermelon naturally occurring in commercial greenhouses.

• 한국식품과학회지
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• 제31권1호
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• pp.20-23
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• 1999

채취시기 및 엽위별 차카데킨의 함량 및 조성에 대한 정보를 얻고자 다엽을 5월, 7월, 8월에 채취하고 다엽을 1엽 심, 2엽, 3엽, 4엽으로 나누어서 카테킨 함량 및 개별 카테킨 조성을 분석한 결과, 채취시기별로는 8월에 채취한 차엽의 카테킨 함량이 5월이나 7월에 채취한 것보다 더 많았으며, 엽위별로는 1엽 심, 2엽, 3엽, 4엽 중에서 1엽 심에 카테킨의 함량이 가장 많고 4엽이 가장 적었다. 또한 구성 차카테킨의 함량은 EGCG가 가장 많은 양이 함유되어 있었으며, ECG, EC, EGC의 순이었다.

• Mycobiology
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• 제48권6호
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• pp.495-500
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• 2020

Leaf spot on lemon balm is frequently observed in Korea, causing considerable damage to crops. In 2014 and 2015, the occurrence of leaf spot was observed in several production greenhouses at Suwon, Gongju, and Namwon in Korea. Symptoms on lower leaves initially developed as small, distinct, discolored lesions, which enlarged progressively turning into dark brown, angular spots surrounded by purplish-brown margins. Based on the morphological characteristics and sequence analysis of actin (ACT), translation elongation factor 1-alpha (EF-1α), internal transcribed spacer (ITS), 28S nrDNA (LSU), and RNA polymerase II second largest subunit (RPB2), the fungus associated with the lemon balm leaf spot was determined as Septoria melissae. To the best of our knowledge, this is the first report of lemon balm leaf spot caused by S. melissae in Asia as well as in Korea.

• 한국초지조사료학회지
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• 제4권3호
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• pp.187-193
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• 1984

Meadow fescue품종(品種)을 공시(供試)하여 엽면적(葉面積)의 수직적분포(垂直的分布)와 건물수량(乾物收量)과의 관계(關係)를 검토(檢討)하였다. 1. 엽면적(葉面積)의 수직적분포(垂直的分布)로서 품종(品種)을 분류(分類)한 결과(結果), 최대엽면적(最大葉面積)이 하층부(下層部)에 분포(分布)되어 상층부(上層部)로 갈수록 엽량(葉量)이 적어지는 품종(品種)(M), 중간층(中間層)에 최대엽면적(最大葉面積)이 분포(分布)되어 상(上) 하층부(下層部)로 갈수록 엽량(葉量)이 감소(減少)되는 품종(品種)(Leto First, Bundy), 최대엽면적(最大葉面積)이 중간층(中間層)에 분포(分布)되어 있으나 상층부(上層部)에 엽량(葉量)이 대부분(大部分)이 집중분포(集中分布)된 품종(品種)(Tammisto) 및 하층부(下層部)에서 최대엽면적(最大葉面積)을 나타내나 전층(全層)에 엽량(葉量)이 비교적(比較的) 균등(均等)히 분포(分布)된 품종(品種)(Trader)의 4 가지 형(型)으로 분류(分類)되었다. 2. 유효엽면적지수는 건물수량(乾物收量), 엽면적지수(葉面積指數), 경중(莖重) 및 경면적지수(莖面積指數)와 유의(有意)한 정상관(正相關)을 나타 내었다. 3. 출수경(出穗莖)이 많은 품종(品種)의 유효엽면적은 상층부(上層部)에 분포(分布)되었으나 영양경(榮養莖)이 많은 품종(品種)은 유효엽면적지수의 비율(比率)이 높았다. 4. 최대엽면적(最大葉面積)을 나타내는 층(層) 이상(以上)에 분포(分布)된 엽면적(葉面積)과 엽면적중(葉面積重)의 관계(關係)는 M과 Trader가 유의(有意)한 부(負)의 상관(相關)이 인정(認定)되었으나 그 외의 품종(品種)에서는 유의(有意)한 상관(相關)이 인정(認定)되지 않았다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.132.3-133
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• 2003

Leaf blight of kudzu ( Pueraria lobata ) was found in Jeonbuk province in 2002. The main symptoms appeared as leaf blight and showed yellowing and wilting. The causal pathogen of the leaf blight was isolated from symptomed kudzu leaf and produced white to cream, usually floccose mycelium. It readily formed reddish orange mycelium on PDA. It produced typical microconidia and macroconidia. The microconidia were the reniform. The macroconidia were wide, slightly curved, usually 3 to 4 septate and size was 45 ∼ 85 ${\times}$ 5 ∼ l0$\mu\textrm{m}$. The pathogen produced chlamydospore singly on short hyphal branches within 2 to 3 weeks, which was hyaline, globose, and smooth walled. The pathogen was, therefore, identified as Fusarium solani based on cultural and morphological characters. This is the first report on the leaf blight of kudzu caused by Fusarium solani in Korea.

• 한국식물병리학회지
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• 제11권1호
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• pp.90-93
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• 1995

Leaf blotch of Welsh onion was observed at a farmer's field at Kangnung in July 1990. Further collection o the diseased materials was made in 1993 and 1994. The symptom initially appeared as discolored spot and later yellowish blotch with the long axis parallel to the leaf veins, which turned to sooty colored concentric lesion due to heavy production of conidiophores and conidia. Cladosporium allii-cepae (Ranojevic) M. B. Ellis was repeatedly isolated from the leaf lesions. Healthy Welsh onion inoculated with conidial suspension of the fungus produced the typical symptom 10 days after inoculation. Koch's postulates were fulfilled by reisolation of the fungus. This is the first report of a Cladosporium leaf blotch of Welsh onion in Korea.

• 한국자동차공학회논문집
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• 제6권6호
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• pp.1-6
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• 1998

A leaf spring plays an important role in a passenger bus. Since characteristic of a leaf spring has a hysteresis behaviour, modeling technique for a leaf spring is an important issue for passenger bus analysis. In this paper, modeling technique for a leaf spring is presented. First, non-linear FEM model of a leaf spring is constructed then it is used to make an approximated model to be used in dynamic analysis. The modeling procedure is ex-plained in step by step approach. Then, this model is applied to dynamic analysis of a mini-bus with flexible body and non-linear dynamic force element. The results are compared with test data.

• The Plant Pathology Journal
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• 제37권4호
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• pp.329-338
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• 2021

Alternaria leaf blight is one of the most common diseases in watermelon worldwide. In Korea, however, the Alternaria species causing the watermelon leaf blight have not been investigated thoroughly. A total of 16 Alternaria isolates was recovered from diseased watermelon leaves with leaf blight symptoms, which were collected from 14 fields in Korea. Analysis of internal transcribed spacer (ITS) region, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and RNA polymerase II second largest subunit (RPB2) were not competent to differentiate the Alternaria isolates. On the contrary, analysis of amplicon size of the histone H3 (HIS3) gene successfully differentiated the isolates into three Alternaria subgroups, and further sequence analysis of them identified three Alternaria spp. Alternaria tenuissima, A. gaisen, and A. alternata. Representative Alternaria isolates from three species induced dark brown leaf spot lesions on detached watermelon leaves, indicating that A. tenuissima, A. gaisen, and A. alternata are all causal agents of Alternaria leaf blight. Our results indicate that the Alternaria species associated watermelon leaf blight in Korea is more complex than reported previously. This is the first report regarding the population structure of Alternaria species causing watermelon leaf blight in Korea.