• 제목/요약/키워드: fig plant

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Detection and Phylogenetic Analysis of Viruses Linked with Fig Mosaic Disease in Seventeen Fig Cultivars in Palestine

  • Jamous, Rana Majed;Zaitoun, Salam Yousef Abu;Mallah, Omar Bassam;Shtaya, Munqez;Elbeaino, Toufic;Ali-Shtayeh, Mohammed Saleem
    • The Plant Pathology Journal
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    • 제36권3호
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    • pp.267-279
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    • 2020
  • Fig mosaic is a viral disease (FMD) that spreads in Palestinian common fig (Ficus carica L.) orchards. Recognizing the economic value of fig plants and the harmful nature of FMD, the disease poses a significant threat to the economy of the fig production in Palestine. We applied the reverse transcription and amplification (RT-PCR) and PCR technique to leaf samples of 77 trees and 14 seedlings of 17 fig cultivars. The samples were collected from orchards in the main fig-growing provinces of the Palestinian West Bank, to assess the prevalence of viruses associated with FMD, and confirm a possible link of symptoms with viruses detected. Four viruses were detected: Fig mosaic virus (FMV), Fig badnavirus-1 (FBV-1), Fig leaf mottle-associated virus 2 (FLMaV-2), and Fig fleck-associated virus (FFkaV). FMV and FBV-1 were found in all tested fig plants (100%), while FLMaV-2 and FFkaV were detected in 61.5% and 33% of the fig samples, respectively. The high incidence of FBV-1 in the newly propagated symptomatic and symptomless seedlings from different cultivars may be an indication that FBV-1 is integrated into the genome of the fig in a cultivar nondiscriminatory manner. Very weak or no association was detected between FMD symptoms severity in the 17 Palestinian fig cultivars with the various viruses' combinations observed (i.e., number of the viruses infecting the plant). These results support the notion that FMD symptom severity expression is likely to be controlled by a combination of FMV infection, cultivars, and environmental factors, rather than the number of viruses infecting the plant.

이산화질소 가스에 의한 무화과 나무의 피해 양상 (The Toxicity of Nitrogen Dioxide Gas on Fig Plant)

  • 김유학;최병렬;김명숙
    • 한국토양비료학회지
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    • 제43권6호
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    • pp.978-980
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    • 2010
  • This study was conducted to observe the cause of injury of fig plant. Nitrogen dioxide gas can be evolved at low pH or reduced in soil. Fig plant cultivated with nutrient solution was wilted or withered. Injury symptom for nutrient solution containing nitrous acid was worse as pH of soil decreased. However, increase in pH of nutrient solution treated with increasing $Ca(OH)_2$ solution prevented nutrient solution from producing nitrogen dioxide gas. Recovery of the fig plant by pH increase indicated that the cause of injury was nitrogen dioxide gas.

Efficacy of Tissue Culture in Virus Elimination from Caprifig and Female Fig Varieties (Ficus carica L.)

  • Bayoudh, Chokri;Elair, Manel;Labidi, Rahma;Majdoub, Afifa;Mahfoudhi, Naima;Mars, Messaoud
    • The Plant Pathology Journal
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    • 제33권3호
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    • pp.288-295
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    • 2017
  • Fig mosaic disease (FMD) is a viral disease that spreads in all Tunisian fig (Ficus carica L.) orchards. RT-PCR technique was applied to leaf samples of 29 fig accessions of 15 fig varieties from the fig germplasm collection of High Agronomic Institute (I.S.A) of ChattMariem, to detect viruses associated to FMD. Analysis results show that 65.5% of the accessions (19/29) and 80.0% (12/15) of the fig varieties are infected by FMD-associated viruses. From all fig accessions, 41.4% of them are with single infection (one virus) and 24.1% are with multi-infections (2 virus and more). Viruses infecting fig leaf samples are Fig mosaic virus (FMV) (20.7%), Fig milde-mottle-associated virus (FMMaV) (17.25%), Fig fleck associated virus (FFkaV) (3.45%), and Fig cryptic virus (FCV) (55.17%). A reliable protocol for FCV and FMMaV elimination from 4 local fig varieties Zidi (ZDI), Soltani (SNI), Bither Abiadh (BA), and Assafri (ASF) via in vitro culture of 3 meristem sizes was established and optimized. With this protocol, global sanitation rates of 79.46%, 65.55%, 68.75%, and 70.83% respectively for ZDI, SNI, BA, and ASF are achieved. For all sanitized varieties, the effectiveness of meristem culture for the elimination of FCV and FMMaV viruses was related to meristem size. Meristem size 0.5 mm provides the highest sanitation rates ranging from 70% to 90%.

Viruses Associated with Fig Mosaic Disease in Different Fig Varieties in Montenegro

  • Latinovic, Jelena;Radisek, Sebastjan;Bajceta, Milija;Jakse, Jernej;Latinovic, Nedeljko
    • The Plant Pathology Journal
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    • 제35권1호
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    • pp.32-40
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    • 2019
  • Symptoms of fig mosaic disease have been noticed on leaves of fig (Ficus carica) for several decades, in Montenegro. In 2014, leaf samples were collected from trees of six fig cultivars in a plantation located in the main fig-producing area of Montenegro, to study the disease. After RNA isolation, samples were tested by RT-PCR for detection of nine fig viruses and three viroids. Four viruses were detected: fig leaf mottle-associated virus 1 (FLMaV-1), fig mosaic virus (FMV), fig mild mottle-associated-virus (FMMaV) and fig badnavirus 1 (FBV-1). Most of the viruses were present in mixed infections. The amplicons of the viruses were directly sequenced from both directions. A BLAST search of these sequences revealed sequence identities with their closest counterparts at GenBank of 92, 97, 92 and 100%, for FLMaV-1, FMV, FMMaV and FBV-1, respectively. Different responses in symptom expression due to the various virus combinations detected have been demonstrated. Variety $Su{\check{s}}ilica$ had the least symptom expression, with only one virus (FBV-1) found. Considering that the production of figs in Montenegro is increasing and has a substantial relevance in this geographic location, the results indicate that more attention should be given to improving the phytosanitary condition of fig trees in the country.

High-Throughput Sequencing Identified Multiple Fig Viruses and Viroids Associated with Fig Mosaic Disease in Iraq

  • Nabeel Al-Kaeath;Shrooq Zagier;Osamah Alisawi;Fadhal Al Fadhal;Naima Mahfoudhi
    • The Plant Pathology Journal
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    • 제40권5호
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    • pp.486-497
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    • 2024
  • Mosaic is the most common viral disease affecting fig plants. Although the Fig mosaic virus is the leading cause of mosaic disease, other viruses are also involved. High-throughput sequencing was used to assess viral infections in fig plants with mosaic. The genomic DNA and total RNAseq of mosaic-symptomatic fig leaves were sequenced using the Illumina platform. The analysis revealed the presence of fig badnavirus 1 (FBV-1), grapevine badnavirus 1 (GBV-1), citrus exocortis viroid (CEVd), and apple dimple fruit viroid (ADFVd). The FBV-1 and GBV-1 sequences were 7,140 bp and 7,239 bp long, respectively. The two genomes encode one open reading frame containing five major protein domains. The viroids, CEVd and ADFVd, were 397 bp and 305 bp long. Phylogenetic analyses revealed a close relationship between FBV-1 and Iranian isolates of the same species, while GBV-1 was closely related to Russian grapevine badnavirus isolates (Tem64, Blu17, KDH48, and Pal9). CEVd was closely related to other Iraqi isolates, while ADFVd was strongly related to a Spanish isolate. A registered endogenous pararetrovirus, caulimovirus-Fca1, with a size of 7,556 bp, was found in the RNA transcripts with a low expression level. This integrant was also detected in the genomes of the two lines 'Horaishi' (a female line) and 'Caprifig 6085' (a male line). Phylogenetic analyses revealed that caulimovirus-Fca1 was distinct from two other clades of different endogenous virus genera.

배양액의 농도가 무화과(Ficus carica L.)의 생육, 수량 및 과실의 품질에 미치는 영향 (Effect of Nutrient Solution Concentration on Growth, Yield and Fruit Quality of Fig Plant (Ficus carica L.))

  • 전하준;황진규;손미자;김민;김정필
    • 생물환경조절학회지
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    • 제15권3호
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    • pp.264-269
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    • 2006
  • 본 실험은 배양액의 농도가 무화과의 생육과 수량에 미치는 영향을 조사하기 위하여 수행하였다. 1차 실험에서 배양액의 농도에 따른 무화과 생육은 1/2농도 처리구에서 초장, 엽수, 경경, 착과수가 양호했으며, 엽장과 엽폭은 1/2농도구를 제외한 다른 처리간에는 차이가 없었다. 배양액의 농도에 따른 과식의 평균과중은 토양재배의 38.4g보다 수경재배에서 50.9g으로 높게 나타났고, 과장, 과경, 당도는 처리간의 차이가 없었다. 수확량은 토양재배보다 수경재배에서 월등하게 많았으며, 배양액 1/2농도보다 2/2와 3/2농도에서 높게 나타났다. 무화과의 수경재배에서 저농도에서 양호한 생육을 나타내었으며, 수량은 높은 농도에서 양호하였다. 본 실험의 결과는 무화과의 수량 및 품질증대에 유용하게 활용될 수 있을 것이다.

THE EFFECTS OF DIFFERENT SHADING OF MULCHING ON YIELD OF ROOT AND QUALITY IN PANAX GINSENG

  • Yang Yeong-yuh
    • 고려인삼학회:학술대회논문집
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    • 고려인삼학회 1974년도 학술대회지
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    • pp.137-146
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    • 1974
  • This experiment was on the purpose to study the effects of different shading of Mulching treatments on the quality and yield of ginseng root. This experiment were conducted at Mei-Feng for one year, from July, 1972 to July, 1973. The variety been used was introduced Korea Panax ginsvng. Three different Shading of Mulching treatments have been studied. The results were summerized as follows: 1. The growth of ginseng plant is good under around 4,300 Lux of light intensity. Fig . showed the shadow treatment of straw had a better effect than that of black or grey plastic film. The differences between treatments were significant. 2. The adequate soil temperature for ginseng culture was in the range of $16-18^{\circ}C$. Fig 2. showed that there were significant differences among treatments, of which the straw shadow treatment had the best effect. 3. The growth of ginseng plant was greatly affected wth various shadow treatments. Fig 1. showed both straw and black plastic film treatments had a better effects on growth of stem, leaf area and leaf numbers. 4. Fig. 2. 3. 4. 5 indicated there were distingished differences among all treatments. The straw and black plastics film mulching treatments had a better effects on root length, root diameter, root weight and leaf weight than the grey plastic film. 5. The amount of plant alkaloids and panacene content had related to the shadow treatment, as showed in Fig. 6 and 7 that straw shadow treatment had greatly increased the procuction of plant alkaloids and panacene content. 6. The quality and yield of roots of ginseng greatly affected by different shading of mulching treatments.

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Occurrence of Anthracnose on Indian Fig Cactus Caused by Glomerella cingulata and Colletotrichum gloeosporioides

  • Kim, Wan-Gyu;Cho, Weon-Dae;Jee, Hyeong-Jin;Hong, Soon-Yeong
    • The Plant Pathology Journal
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    • 제16권5호
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    • pp.294-296
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    • 2000
  • Anthracnose symptoms were frequently found on stems of Indian fig cactus in Cheju island of Korea in 1998 and 1999. Typical symptoms were gray to black dry rot of stems with concentric arrays of dot-like spots. A Glomerella sp. or a Colletotrichum sp. was frequently isolated from the symptoms, both of which were identified as Glomerella cingulata and its anamorph, Colletotrichum gloeosporioides based on their morphological and cultural characteristics. Out of 31 isolates obtained from the symptoms, 12 isolates were the anamorph producing only conidia, four isolates the telemorph producing only ascospores, and 15 isolates the holomorph producing both spores on potato dextrose agar. Stem rots similar to the original anthracnose symptoms were induced by wound inoculation of conidia and ascospores but not by non-wound inoculation. The anamorphic isolates caused more extensive stem rot than the telemorphic and holomorphic ones.

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Development of transgenic disease-resistant root stock for the growth of watermelon

  • Cho, Song-Mi;Chung, Soo-Jin;Moon, Sun-Jin;Kim, Kwang-Sang;Kim, Young-Cheol;Cho, Baik-Ho
    • 한국식물생명공학회:학술대회논문집
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    • 한국식물생명공학회 2004년도 생명공학 실용화를 위한 비젼
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    • pp.62-65
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    • 2004
  • To protect the watermelon against soil-borne pathogens, we are currently producing disease-resistant transgenic root stock for the growth of watermelon, A defensin gene (J1-1) from Capsicum annum, a ACC deaminase gene from Pseudomonas syringae, a galactinol synthase (CsGolS) gene from Cucumis sativus, and a WRKY (CvWRKY2) gene from Citullus vulgaris were used as transgenes for disease resistance. The gene were transformed into a inbred line (6-2-2) of watermelon, Kong-dae watermelon and a inbred line (GO702S) of gourd, respectively, by Agrobacterium-mediated transformation. Putative transgenic plants were selected in medium containing 100mg/L kanamycin, and then integration of the genes into the genomic DNA were demonstrated by PCR analysis. Successful integration of the gene in regenerated plants was also confirmed by PCR (Figf 1), genomic Southern blot (Fig 2), RT-PCR (Fig 3), and Northern blot analysis(Fig 4). Several T1 lines having different transgene were produced, and disease resistance of the T1 lines are under estimation.

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Studies on the Fruiting Phase of Rape Under the Different Cultural Conditions

  • Kae, B.M.
    • 한국작물학회지
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    • 제12권
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    • pp.77-87
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    • 1972
  • The fruiting phase of rape under transplanting and direct-sowing conditions has been studied at Mokpo during the 2 years period from 1970 to 1971. Two varieties, Yudal and Miyuki were used in this study. The planting space and sowing time were also incorporated into this study. The results could be summarized as follows: 1. The plant tape of rape was nearly umbrella-shaped of all, but has changed to the laid elliptical-shaped, broadly ovate and spindle-shaped under different varieties and cultural conditions in the plant diagram(Fig. 2). 2. The length of the primary branches for each nodes had a tendency to the symmetric apical curve with the apex at the upper 10-12th node in the transplanting. but to the upper bias apical curve with the apex at the upper 5-7th node in the dense-sowing(Fig.3). 3. The ear of main stem was longer, more pods, heavier 1, 000 grains and more grain yield than ear of primary branches of all, Especially, as for that, the rate of yield constitution per plant in the direct-sowing was higher than in the transplanting(Fig. 4, 5, 6, 7, 8, 9). 4. The ear-length of the primary branches for each nodes had a tendency to the relatively slowly apical curve with the apex at the upper 3-4th node in the transplanting, but to the lower bias apical curve with the apex at the upper 2nd node in the dense-sowing. Especially, the possibility of growth at the lower ears was few in the early variety (Fig. 4). 5. The number of pod per ear on the primary branches for each nodes had a .tendency to the curve of ear-length with the apex at the upper 5-8thnode in the transplanting and at the upper 4-5th node in the dense-sowing (Fig. 5). Accordingly, a high positive correlation was found between the ear-length and number of pod per ear (Table 2) 6. In the transplanting, the high rate of effective ear was from the upper nods to the 12th node, but below the 16-17th nodes was ineffective. However, in the early dense-sowing the high rate of effective was to the 7th node. but below the 10th nodes was. ineffective. Especially, in the early variety has difficult to secure of poi-numbers for ineffective of the lower nodes(Fig. 6.). 7. The density of pod setting of the ear of main stem was the longest of all ears, and the lower nods were, the shorter it became. That had a tendency to the evidently apical growth. However. in the early variety, it was lengthened according to growth of ear-length(Fig.7). 8. The pod-length of the medium nodes was longer than the upper and lower, and the possitive correlation between pod-length and number of grain per poi was very high(Table 2.). 9. In the grain yield per node of primary branches, the most yielding node of transplanting was the upper 9th node, of dense-sowing 4-5th node(Fig 8.), and the possitive correlation between grain yield per node and ear-length or number of pod per ear was very high(Table 2). 10. The grain yield of ear of main stem was higher than that of primary branches in the percentage of dependence for grain yield per plant. The limint node of 50% of dependence to cumulative grain yield per plant was the upper 7-8th node in tranplanting, in the early dense-sowing 4-5th node, and in tke late dense-sowing-3th node(Fig. 9). 11. In the weight of 1, 000 grains the lower nodes were, the lighter it becames in dense-sowing. Therefore, this was also lighter than in the transplanting to the (Fig. 10.). 12. The oil content of grain at the medium nodes was low in the early variety, but at the ear of main stem and upper 1st node it was extremely high(Fig. 11.).

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