This study was conducted to investigate the response of growth and yield of rice under five different cultural methods, machine transplanting(MTNT), puddled drill seeding (PDSNT), drill seeding on soil surface (DSNT) , broadcasting on soil surface (BSNT) under no-tillage paddy condition and conventional machine transplanting(MTT) in Jeonbuk series(siltyloam soil) from 1993 to 1995. Soil hardness was higher in no-tillage soil and increased with highly difference between tillaged and no-tillage soil with deeper soil depth. Bulk density was heavier in no-tillage soil and porosity was higher in tilled soil than that of the control. The rate of effective tiller was higher in MTT, following MTNT, PDSNT, DSNT and BSNT. Weed occurrence was more serious in no-tillage soil, than that of tillaged soil. The rate of lower internode length was lower in DSNT and BSNT and was similar with MTT in PDSNT and MTNT. Height of center gravity in terms of lodging tolerance was lower in direct seeding than in machine transplanting. Depth of buried culm was shorter in no-tillage soil, especially in DSNT and BSNT. Total amount of root was higher in MTT, following MTNT, PDSNT, BSNT and BSNT and the distribution rate of root in shallower soil layer was higher in no-tillage soil, especially in BSNT and DSNT. Field lodging occured highly in BSNT, following DSNA, PDSNT and MTNT with high lodging scale in DSNT and BSNT. Panicle number per unit land square meter was the highest in MTT and the least in BSNT. Ripened grain ratio was low in BSNT and DSNT due to heavy lodging. Yield of milled rice was 93% in PDSNT, 87% in DSNT, 81% in BSNT and 96% in MTNT, compared with 534kg /10a in MTT.
Soybean is the most promising crop for substituting rice on the paddy field. Excessive water stress is a common limiting factor in soybean yield under paddy soil condition. This study was carried out to identify changes in leaf total nitrogen and petiole ureide content under excess water conditions for establishing a screening system related to waterlogging tolerance. Waterlogging treatment was conducted by maintaining the water level on the soil surface for 10 days at the early vegetative growth stage ($V_5$) and the flowering stage ($R_2$). Leaf total nitrogen content, SPAD value and ureide content in petiole decreased in all soybean varieties in response to waterlogging, but the degree of decrease was much lesser in Pungsannamulkong and Muhankong than in Jangyeobkong and Myungjunamulkong, at 21 days after waterlogging treatment. This result means that root and nodule recovery rates were much higher in Pungsannamulkong and Muhankong than in Jangyeobkong and Myungjunamulkong after waterlogging treatment. The ureide and leaf nitrogen content showed high positive correlation with SPAD value, regardless of waterlogged stages. In conclusion, leaf nitrogen content, ureide content in petiole and leaf greenness were identified as promising indicator for screening soybeans which are tolerant of excess water.
Global climatic change and increasing climatic instability threaten crop productivity. Due to climatic change, drought stress is occurring more frequently in crop fields. In this study, we investigated the effect of treatment with hydrogen peroxide (H2O2) before leaf development on the growth and yield of sorghum for minimizing the damage of crops to drought. To assess the effect of H2O2 on the growth of sorghum plant, 10 mM H2O2 was used to treat sorghum leaves at the 3-leaf stage during growth in field conditions. Plant height, stem diameter, leaf length, and leaf width were increased by 7.6%, 9.6%, 8.3% and 11.5%, respectively. SPAD value, chlorophyll fluorescence (Fv/Fm), photosynthetic rate, stomatal conductance, and transpiration rate were increased by 3.0%, 4.9%, 26.0%, 23.4% and 12.7%, respectively. The amount of H2O2 in the leaf tissue of sorghum plant treated with 10 mM H2O2 was 0.7% of the applied amount after 1 hour. The level increased to approximately 1.0% after 6 hours. The highest antioxidant activity measured by the Oxygen Radical Absorbance Capacity assay was 847.3 µmol·g-1 at 6 hour after treatment. However, in the well-watered condition, the concentration of H2O2 in the plant treated by the foliar application of H2O2 was 227.8 µmol·g-1 higher than that of the untreated control. H2O2 treatment improved all the yield components and yield-related factors. Panicle length, plant dry weight, panicle weight, seed weight per plant, seed weight per unit area, and thousand seed weight were increased by 8.8%, 18.0%, 24.4%, 24.7%, 29.9% and 7.1%, respectively. Proteomic analysis showed that H2O2 treatment in sorghum increased the tolerance to drought stress and maintained growth and yield by ameliorating oxidative stress.
Purpose : To evaluate the effectiveness and tolerance of postoperative e지ernai beam radiotherapy for patients with low grade glioma of the brain and define the optimal radiotherapeutic regimen. Materials and Methods : Between June, 1985 and May, 1998, 72 patients with low grade gliomas were treated with postoperative radiotherapy immediately following surgery. Median age was 37 years with range of 11 to 76 years. Forty one patients were male and 31 patients were female with male to female ratio of 1.3:1. Of those patients, 15 underwent biopsy alone and remaining 57 did subtotal resection. The distribution of the patients according to histologic type was as follows: astrocytomas-42 patients (58$\%$), mixed oligodendrogliomas-19 patients (27$\%$), oiigodendrogliomas-11 patients (15$\%$). Two patients were treated with whole brain irradiation followed by cone down boost and remaining 70 patients were treated with localized field with appropriate margin. Ail of the patients were treated with conventional once a day fractionation. Most of patients received total tumor dose of 5000 $\~$ 5500 cGy. Results : The overall 5 and 7 year survival rates for entire group of 72 patients were 61$\~$ and 50$\~$. Corresponding disease free survival rates for entire patients were 53$\~$ and 45$\~$, respectively. The 5 and 7 year overall survival rates for astrocytomas, mixed oligodendrogiiomas, and oligodendrogiiorras were 48$\%$ and 45$\%$, 76$\%$ and 56$\%$, and 80$\%$ and 52$\%$, respectively. Patients who underwent subtotal resection showed better survival rates than those who did biopsy alone. The overall 5 year survival rates for sub total resection patients and biopsy alone patients were 57$\%$ and 43$\%$, respectively. Forty six patients who were 40 years or younger survived batter than 26 patients who were 41 years or older (overall survival rate at 5 years, 69$\%$ vs 45$\%$). Although one patient was not able to complete the treatment because of neurological deterioration, there was no significant treatment related acute toxicities. Conclusion : Postoperative radiotherapy was safe and effective treatment for patients with low grade gliomas. However, we probably need prospective randomized trial to define optimal treatment timing and schedule for low grade gliomas and select patient group for different treatment philosophies.
The highland area remained bare for the winter and spring seasons and this condition has resulted in soil erosion. In this areas, crop rotation by legume has not been commonly practiced. Thus, this study was carried out to evaluate the effects of the growth characteristics and nitrogen yield as a green manure crop according to sowing seasons of hairy vetch and woolly pod vetch. In this experiment, we was investigated the growth characteristics, winter tolerance, soil coverage and productivities of hairy vetch and woolly pod vetch. We cultivated two hairy vetch(Madison, H1) and two woolly pod vetch(Naomi, Haymaker) varieties in the highland area from 2006 to 2007. The result are summarized as follows; The plant height of hairy vetch increased with the progress of growth stage until flowering stage. The wintering rate of hairy vetch was $79{\sim}83%$ in highland area. But, the wintering rate of woolly pod vetch was $29.5{\sim}39.2%$. The 100% soil coverage of hairy vetch was reached between end of May and beginning of June under autumn sowing, and between end of June and beginning of July under spring sowing. The weeds density of hairy vetch field under autumn sowing was lower than that under spring sowing. Dry matter yield of hairy vetch was significantly higher than that of woolly pod vetch under autumn sowing. The dry matter yield of hairy vetch and woolly pod vetch were $5,255{\sim}5,405kg/ha$ and $3,520{\sim}3,640kg/ha$, respectively. But, hairy vetch in spring sowing was not significantly higher fresh matter yield than that of woolly pod vetch. The nitrogen content in hairy vetch and woolly pod vetch was $2.98{\sim}3.08$ and $2.74{\sim}3.21%$, respectively. There were no significant difference in nitrogen content of the hairy vetch and woolly pod vetch under the spring sowing. N-uptake of hairy vetch was significantly higher than that of woolly pod vetch in autumn sowing. The N uptake was $156{\sim}164\;kg/ha$ in hairy vetch and $96{\sim}101\;kg/ha$ in woolly pod vetch under autumn sowing. It was thought that hairy vetch was better than woolly pod vetch as a green manure crop in autumn sowing considering wintering rate and yield of dry matter in highland area. And woolly pod vetch could use as a green manure crop under spring sowing in highland area.
Three species of Fusarium, F. fujikuroi, F. verticillioides and F. proliferatum, are known to be associated with bakanae disease of rice [1, 2]. F. fujikuroi infects rice flowers and survive in endosperm and embryo of the seeds. Infected seed is an important source of primary inoculum of pathogens [3]. Seeds of rice (Oryza sativa cv. Boramchan) collected from bakanae-infected field were found to be 96% infected with Fusarium sp., 52% with F. fujikuroi, 42% with F. verticillioides, and 12% with F. proliferatum as determined by incubation method and species-specific PCR assays. F. fujikuroi was detected at lemma/palea, endosperm and embryo whereas F. verticillioides and F. proliferatum were recovered only from lemma/palea by means of component plating test. Seed disinfection methods have been developed to control bakanae disease and prochloraz has been most widely used for rice seeds. Two chemicals formulated with prochloraz (PC 1) and prochloraz + hexaconazole (PC 2) that inhibit biosynthesis of ergosterol strongly reduced the incidence of Fusarium spp. on selective media to 4.7% and 2.0%, respectively. Disease symptoms of rice seedlings in nursery soil were alleviated by chemical treatment; seedlings with elongated leaves or wide angle between leaf and stem were strikingly reduced from 15.6 to 3.2% (PC 1) and 0 (PC 2), stem rots were reduced from 56.9 to 26.2% (PC 1) and 32.1% (PC 2), and normal seedling increased from 0.4 to 13.3% (PC 2). Prochloraz has some disadvantages and risks such as the occurrence of tolerant pathogens [4] and effects on the sterol synthesis in animals and humans [5]. For these reasons, it is necessary to develop new disinfection method that do not induce fungal tolerance and are safe to humans and animals. Chlorine dioxide ($ClO_2$), that is less toxic, produces no harmful byproducts, and has high oxidizing power, has been reported to be effective at disinfection of several phytopathogenic fungi including Colletotrichum spp. and Alternaria spp. [6]. Gaseous $ClO_2$ applied to rice seeds at a concentration of 20 ppm strongly suppressed mycelial growth of Fusarium fujikuroi, F. verticillioides and F. proliferatum. The incidence of Fusarium spp. in dry seed with 8.7% seed moisture content (SMC) tended to decrease as the concentration of $ClO_2$ increased from 20 to 40 ppm. Applying 40 ppm $ClO_2$ at 90% relative humidity, incidence was reduced to 5.3% and resulted in significant reduction of disease symptoms on MS media. In nursery soil, stem rot was reduced from 56.9 to 15.4% and the number of normal seedlings increased from 0.4 to 25.5%. With water-soaked seeds (33.1% SMC) holding moisture in the endosperm and embryo, the effectiveness of disinfection using $ClO_2$ increased, even when treated with only 20 ppm for four hours. This suggests that moisture was a key element for action of $ClO_2$. Removal of the palea and lemma from seeds significantly decreased the incidence of Fusarium spp. to 3.0%. Seed germination appeared to decrease slightly by water-soaking at $30^{\circ}C$ because of increased SMC and by physical damage of embryos from hulling. These results indicate that the use of gaseous $ClO_2$ was effective as a means to disinfect rice seeds infected with Fusarium spp. and that moisture around the pathogens in the seed was an important factor for the action of $ClO_2$. Further investigations should be conducted to ascertain the best conditions for complete disinfection of Fusarium spp. that infect deep site of rice seeds.
Several wild yeasts were isolated from Korean grape varieties before and during spontaneous fermentation. Among them, four strains were isolated based on the alcohol content and flavor production in wine after fermentation of apple juice. In this study, the four yeast strains were identified and characterized. PCR-restriction fragment length polymorphism analysis of ITS I-5.8S-ITS II region with restriction endonuclease Hae III and Hinf I resulted in that all the strains showed a typical pattern of Saccharomyces cerevisiae. Pulse field gel electrophoresis showed three different chromosome patterns with a same band between strains SS89 and SS812. When ITS I-5.8S-ITS II sequences of the four strains were compared with one another, they were similar to those of Saccharomyces cerevisiae CBS 4054 type strain. Identity of the sequences was higher than 97% with those of the type strain. Phylogenetic analysis showed based on the sequences showed they were genetically closed to the type strain. The four identified strains were tested in a medium containing 200 ppm potassium metabisulfite, and the MM10 and WW108 inhibition rates resulted at up to 24 h. The four strains were tested at an incubation temperature of $30^{\circ}C$. The 30% sugar concentration in the medium (w/v) showed the highest growth in 36 h, especially in the case of SS89, which was close to growth 40. The four strains were tested in an 8% ethanol medium (v/v). Alcohol tolerance was initially kept in the incubation process. The strains began to adapt, however, to the exceeded resistance. The four strains showed the lowest inhibition rate at 24 h.
Effect of flooding on the desalting efficiency and the growth of sudan grass, barnyard grass, sesbania and corn was studied in a sandy soil of the Iweon reclaimed tidal land. Flooding plots were treated by 400 (one time flooding), 800 (two times flooding), and 1,200 mm(three times flooding) of water, respectively, and then soil salinities of the treated plots were compared with salinity of the control plot (not flooded) for estimation of desalting effect. Desalting ratio of 1,200 mm treatment was 78.3% for depth 0-20 cm, 70.5% for depth 20-40 cm and 60.8% for depth 40-60 cm, and then the soil salinity reached at 3~6 dS $m^{-1}$. Consequently, it was considered that sandy saline soil was satisfactorily desalted for upland crops to be cultivated by 1,200 mm flooding, but insufficiently desalted by 400 mm and 800 mm flooding because of high salinity ranged 5~14 dS $m^{-1}$ even after flooding treatment. In addition, it was estimated that soil salinity should be controled lower than 7.7 dS $m^{-1}$ in order to obtain more than 80%of crop emergence when four crops are simultaneously cultivated by inter- or mixed cropping in a field. Dry matter yields (kg $10a^{-1}$) was 1,068 for sudan grass, 696for barnyard grass, 1,426 for sesbania, and 1,164 for corn by 1,200 mm flooding treatment, but only 46.8~74.3% by 800 mm flooding treatment and 2.9~25.5% by 400 mm flooding treatment. Therefore, it is concluded that the flooding treatment more than 1,200 mm is necessary for satisfactory desalinization in order for the low salt tolerance crops to be cultivated in the sandy reclaimed tidal land.
Journal of The Korean Society of Grassland and Forage Science
/
v.32
no.3
/
pp.193-202
/
2012
'Joseong', a winter forage triticale cultivar (X Triticosecale Wittmack), was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2010. The cultivar 'Joseong' was selected from the cross FAHAD_5/RHINO1R.1D 5+10 5D'5B'//FAHAD_5 by CIMMYT (Mexico) in 1992. Subsequent generations were handled in pedigree selection program at Mexico from 1993 to 1998, and a line 'CTSS92Y-A-4Y-0M-5Y-0B' was selected for earliness and good agronomic characteristics. After preliminary and advance yield test in Korea for 2 years, the line designated as a line name of 'Iksan26'. The 'Iksan26' was subsequently evaluated for earliness and forage yield in 7 different locations such as Jeju, Iksan, Cheongwon, Yesan, Naju, Daegu, and Jinju from 2008 to 2010 and finally named as 'Joseong'. The cultivar 'Joseong' has characteristics of dark green leaf, yellow culm and spike, and large grain of yellowish brown color. The heading date of cultivar 'Joseong' was April 24, which was 5 days earlier than that of check cultivar 'Shinyoung'. It showed better tolerance or resistance to lodging, wet injury, powdery mildew, and leaf rust than those of the check cultivar 'Shinyoung'. The average forage dry matter yield of cultivar 'Joseong' at milk-ripe stage was 14.5 MT $ha^{-1}$, which was lower than 16.5 MT $ha^{-1}$ of the check cultivar 'Shinyoung'. The silage quality of 'Joseong' (5.3%) was lower to the check cultivar 'Shinyoung' (6.0%) in crude protein content, while was 2.1% superior to the check cultivar 'Shinyoung' (61.9%) in TDN (total digestible nutrients). It showed 5.1 MT $ha^{-1}$ of grain yield, which was 40% higher than that of the check cultivar 'Shinyoung' (3.65 MT $ha^{-1}$). This cultivar is recommended for fall sowing crop in the area where daily minimum mean temperatures are averaged higher than $-10^{\circ}C$ in January, and as a winter crop using whole crop forage before planting rice in around Korea.
A new covered barley variety, "Geungangbori" was developed from the cross between Milyang 55 which have lodging tolerance and easy brittleness and Suweon 260 with good quality by barley breeding team in the Yeongnam Agricultural Research Institute (YARI) in 2002. A promising line, YMB3855-3B-14-1-1-1, was selected in 1999. It was designated as the name of Milyang 110. It was prominent and had good result from regional adaptation yield trials (RAT) for three years from 2000 to 2002 and released as the name of "Geungangbori". Geungangbori is resistant to barley yellow mosaic virus and moderately resistant to powdery mildow. The average maturing date was same with Olbori on paddy field in regional adaptation yield trials for 2000-2002. Its culm length is 17 cm shorter than that of Olbori and the spike length is 4.4cm, it's longer than olbori. The 1,000 grain weight of Geungangbori was 34 g, same as Olbori, but the number of spikes per $m^2$ and test weight ware lower than those of Olbori. The yield potential of Geungangbori was 4.22 MT/ha on paddy in regional adaptation yield trials for 2000-2002. which was 7% higher than that of Olbori. The cooking quality of Geungangbori were similar to Olbori such as water absorption rate and expansion rate. But the crude protein content is lower than Olbori. This variety is suitable for double cropping system with rice in the southern part of the Korean Peninsula.
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