• Asian-Australasian Journal of Animal Sciences
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• v.29 no.4
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• pp.457-463
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• 2016

The goal of this work was to investigate the correlations between MyHC mRNA transcription and their corresponding protein expressions in porcine longissimus muscle (LM) during postnatal growth of pigs. Five DLY ($Duroc{\times}Landrace{\times}Yorkshire$) crossbred pigs were selected, slaughtered and sampled at postnatal 7, 30, 60, 120, and 180 days, respectively. Each muscle was subjected to quantity MyHCs protein contents through an indirect enzyme-linked immunosorbent assay (ELISA), to quantity myosin heavy-chains (MyHCs) mRNA abundances using real-time polymerase chain reaction. We calculated the proportion (%) of each MyHC to total of four MyHC for two levels, respectively. Moreover, the activities of several key energy metabolism enzymes were determined in LM. The result showed that mRNA transcription and protein expression of MyHC I, IIa, IIx and IIb in LM all presented some obvious changes with postnatal aging of pigs, especially at the early stage after birth, and their mRNA transcriptions were easy to be influenced than their protein expressions. The relative proportion of each MyHC mRNA was significantly positively related to that of its corresponding protein (p<0.01), and MyHC I mRNA proportion was positively correlated with creatine kinase (CK), succinate dehydrogenase (SDH), malate dehydrogenase (MDH) activities (p<0.05). These data suggested that MyHC mRNA transcription can be used to reflect MyHC expression, metabolism property and adaptive plasticity of porcine skeletal muscles, and MyHC mRNA composition could be a molecular index reflecting muscle fiber type characteristics.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.47 no.6
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• pp.123-129
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• 2015

There are two kinds of cartons for beverage packaging, one is aseptic brick (AB) type and the other is gable top (GT). In this study, AB and GT were used as a raw material of recycled paper to investigate the recyclability at their varied blending ratios. Fiber consistency at pulping decreased as the blending ratio of AB increased. As a result, a lot of fines were generated from AB and flakes from GT increased because shear force in pulper decreased. Bulk of handsheets was more than $2.0cm^3/g$, and ISO brightness decreased as the blending ratio of AB increased. The best condition to recycle beverage cartons is to discriminate each cartons separately because of differences in the composition. However, there are problems such as the limit of the collection system and social costs. Therefore, it is assumed that the blending ratios of AB should be adjusted at less than 20% for effective recycling of beverage cartons.

• Resources Recycling
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• v.10 no.4
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• pp.34-41
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• 2001

A research was performed to evaluate a use of shredder residue to currently dispose of at landfills. Laboratory analyses were conducted to determine especially the fuel characteristics of shredder residue. For this aim, shredder residue was classified by the particle size as well as by the type of material and the content of Cl, S, ash, and calorific value were determined. Due to the chlorinated plastic content of shredder residue, mean concentration of Cl was found to exceed 4wt% except one sample while that of S was ranged from 0.25 to 0.39 wt%. As far as calorific value was concemed, plastic was observed to be more than 10,000 kcal/kg while wood/paper and fiber accounted for approximately 4,000 kcal/kg. Shredder residue was found to contain varying trace amounts of metal elements, including Fe of 6∼8.5 wt%. Hg and Cr(VI) were not detected, however, while Cd was contained as small as 0.0004-0.0009 wt%.

• The Korean Journal of Food And Nutrition
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• v.34 no.4
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• pp.372-380
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• 2021

In this study, data from the 7th (2016~2018) and 8th (2019) Korea National Health and Nutrition Examination Surveys were used, which included 5,325 subjects. Health behavior, dietary and nutrient intake status, physical measurement and biochemical characteristics, and risk factors for elderly related chronic diseases were classified and analyzed according to the changing composition of single households and other households in the current society. As a result, the ratio of current smokers and drinkers in young adult single households, walking less than 30 minutes per day, subjective health status was poor, breakfast rate less than three times per week, eating out frequency more than once a day, lipid intake ratio to total calories, saturation fatty acid intake were significantly higher. In addition, waist circumference, and diastolic blood pressure were significantly higher. However, dietary fiber intake level was significantly lower. The results for hypertension, which is the representative chronic disease that causes old age-related chronic diseases, were significantly higher in single households (ORs=1.400 (95% CI: 1.095, 1.791), p=0.007). Although young adults may not have showed particularly serious health problems yet, education is believed as important to recognize and prevent age-related disease risk factors.

• Journal of Ginseng Research
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• v.43 no.3
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• pp.475-481
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• 2019

Background: The ginsenoside Rg1 has been shown to exert various pharmacological activities with health benefits. Previously, we have reported that Rg1 promoted myogenic differentiation and myotube growth in C2C12 myoblasts. In this study, the in vivo effect of Rg1 on fiber-type composition and oxidative metabolism in skeletal muscle was examined. Methods: To examine the effect of Rg1 on skeletal muscle, 3-month-old mice were treated with Rg1 for 5 weeks. To assess muscle strength, grip strength tests were performed, and the lower hind limb muscles were harvested, followed by various detailed analysis, such as histological staining, immunoblotting, immunostaining, and real-time quantitative reverse transcription polymerase chain reaction. In addition, to verify the in vivo data, primary myoblasts isolated from mice were treated with Rg1, and the Rg1 effect on myotube growth was examined by immunoblotting and immunostaining analysis. Results: Rg1 treatment increased the expression of myosin heavy chain isoforms characteristic for both oxidative and glycolytic muscle fibers; increased myofiber sizes were accompanied by enhanced muscle strength. Rg1 treatment also enhanced oxidative muscle metabolism with elevated oxidative phosphorylation proteins. Furthermore, Rg1-treated muscles exhibited increased levels of anabolic S6 kinase signaling. Conclusion: Rg1 improves muscle functionality via enhancing muscle gene expression and oxidative muscle metabolism in mice.

• Korean Journal of Community Nutrition
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• v.28 no.4
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• pp.282-292
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• 2023

Objectives: Bone health in early adulthood, as individuals approach peak bone mass, plays a critical role in preventing osteoporosis later in life. This study aimed to investigate the associations between lifestyle and dietary factors, anthropometric measurements, and urinary bone resorption markers in young adults. Methods: A cross-sectional study was conducted with 100 healthy Korean adults (50 men and 50 women) in their 20s and early 30s. Bone mineral density (BMD), anthropometric measurements, dietary intake (24-hour recall), and urinary bone resorption indicators (deoxypyridinoline and N-terminal telopeptide of type I collagen) were analyzed. Variables were compared between the osteopenia and osteoporosis groups (OSTEO group: 30% men and 60% women) and the healthy control group. Results: Men in the OSTEO group were significantly taller than those in the control group (P < 0.05). Women in the OSTEO group had significantly lower body weight and body composition (muscle and body fat) than those in the normal group (P < 0.01). Men in the OSTEO group had a significantly higher intake of animal calcium (Ca) than those in the normal group (P < 0.05). Women in the OSTEO group had significantly higher dietary fiber, vitamin A, Ca, plant Ca, and potassium intake than did those in the normal group (P < 0.05). There were no significant differences in caffeinated beverage consumption, eating habits, or urinary bone resorption indicators between the OSTEO and control groups of either sex. Conclusions: In our study of young South Korean adults, we observed low bone density levels, with particularly low BMD in taller men and underweight women. We found a higher nutrient intake in the OSTEO group, indicating the possibility of reverse causality, a phenomenon often found in cross-sectional studies. Therefore, there is a need to further elucidate dietary factors related to osteoporosis in young adults through prospective cohort studies involving a larger population.

• Applied Biological Chemistry
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• v.39 no.4
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• pp.268-273
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• 1996

Fundamental data for new type of product development from Lycii fructus was experimentally determined. The chemical composition, and Hunter value changes, optimum extraction, volatile components of extract under different extraction conditions and heat treatment were mainly studied. Results are summarized as follows. The proximate compositions of dried Lycii fructus were water 21.8%, total sugar 27.6%, reducing sugar 15.2%, crude protein 14.29%, crude fat 5.65%, crude fiber 7.48%, and ash 7.98% in percent stale, respectively. Extracted yield on the basic of solid extract was getting increased when more solvent was used for extraction. The most recommendable extraction was 1 to 10 part of sample to solvent ratio. When water was employed as extraction solvent, the highest amount of solid extract was obtained. Extract of Lycii fructus in terms of yields and color was most acceptable when raw sample was treated 8 minute roasting at $180^{\circ}C$ and 60 minute heating at $120^{\circ}C$, respectively. Major volatile components of fresh Lycii fructus were to hexadecanoic acid, methyl linoleate, benzyl alcohol, dimetane benzene by GC/MS. By the roasting of raw sample, the compounds of 2-methyl-2buthenal, 1,4-dimethyl benzene, and benzyl alcohol were reduced. Wheras, methyl thiopropanol, benzene acetaldehyde and ethyl linoleate were slightly increased.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.4
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• pp.325-332
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• 2016

The objective of this study was to determine the growth characteristics of cool ($C_1$) and warm season grasses ($C_2$) in pastures mixed with $C_1$ and $C_2$ suitable for grazing horses and effect of pastures mixed with tall and short grasses on the intake characteristics of horses. $C_1$ used in this study was Kentucky bluegrass, Redtop (short type grass) and tall type grasses were orchardgrass and tall fescue, respectively. The short type grass used as $C_2$ was Bermudagrass. This study had the following four treatment groups: 1) Treatment 1 (Bermudagrass + Kentucky bluegrass + Redtop) 2) Treatment 2 (Bermudagrass + tall fescue + orchardgrass) 3) Treatment 3 (Kentucky bluegrass + Redtop) 4) Treatment 4 (tall fescue + orchardgrass). There was no winterkilling or lodging problem at an altitude of 200 m. Plant heights in mixed pasture of Treatment 1, 2, 3, and 4 were 53.9, 58.2, 57.5, and 78.1 cm, respectively. Plant height was the highest in Treatment 4. Dry matter yield was in the following order : Treatment 4 > Treatment 3 > Treatment 2 > Treatment 1. In the first investigation regarding vegetation distribution, Bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 70% and 66.7%, respectively. Overall, other grasses showed poor growth. In the second investigation of vegetation distribution, Bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 80% and 60.7%, respectively. Crude protein content, neutral detergent fiber content, acid detergent fiber content, digestibility value, and nutritive values were the lowest in Treatment 4, followed by those in Treatment 1, Treatment 2 and Treatment 3.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.10
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• pp.1378-1384
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• 2006

A survey was conducted for 221 male and 191 female college students to study correlations between dietary food intakes and body composition. Among the subjects 75 male and 82 female students were analyzed body protein mass, body mineral mass, percent body fat, waist-hip ratio (WHR), body mass index (BMI) and fitness score using Inbody 3.0. The results show that 94.6% of the males and 24.7% of the females were observed at high level in body protein mass. As for body mineral mass, 86.4% of the males were observed below the normal range. As for WHR, 32.1% of the males and 43.5% of the females were evaluated as abdominal obesity. As for BMI, 34.8% of the males were observed above the normal range. In female students, correlation coefficient between sodium intakes and age showed significantly negative 0.232 while correlation between calcium intakes and self-reported health status showed significantly positive 0.234. In males, correlation coefficient between fiber intakes and self-reported health status resulted in significantly positive 0.237 and that between BMI and age showed significantly positive 0.276. In females, correlation coefficient between type of living and WHR showed significantly positive 0.253 and that between self-reported health status and percent body fat showed significantly positive 0.230. Also correlation coefficient between body protein mass and sodium intakes showed significantly negative 0.276 in female students.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.1
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• pp.19-27
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• 2017

The objective of this study was to determine the growth characteristics of cool (C1) and warm season grasses (C2) in pastures mixed with C1 and C2 at an altitude of 400 m in Jeju island to establishing pasture suitable for grazing horses and to evaluate the effect of pastures mixed with tall and short type grasses on the intake characteristics of horses. C1 used in this study was Kentucky bluegrass, redtop (short type grass) and tall type grasses were orchardgrass and tall fescue, respectively. Treatments of this study were consisted of four groups and the short type grass used in pastures mixed with C1 and C2 was mainly bermudagrass. Four treatment groups were follow as; Treatment 1 (bermudagrass + Kentucky bluegrass + redtop) 2) Treatment 2 (bermudagrass + tall fescue + orchardgrass) 3) Treatment 3 (Kentucky bluegrass + redtop) 4) Treatment 4 (tall fescue + orchardgrass). Bermudagrass was a little winter killing and inhibition of plant growth at an altitude of 400 m. Plant heights in pastures mixed with C1 and C2 were grown better than that in pastures mixed with C1. Especially, plant height in Treatment 4 was higher than other treatments. Dry matter yield was in the following order: Treatment 4> Treatment 3> Treatment 2> Treatment 1. Dry matter yield in pastures mixed with C1 increased as compared with pastures mixed with C1 and C2. Dry matter yield in Treatment 3 was higher than other treatments. In the first investigation regarding vegetation distribution, bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 11.7 and 13.3%, respectively. The growth of bermudagrass in winter was low due to the cold damage. However the growth of Kentucky bluegrass, redtop, tall fescue and orchardgrass was good. In the second investigation, bermudagrass ratios among grasses in Treatment 1 and Treatment 2 were 5.0 and 11.7%, respectively. Growth of forage in the second investigation was poor as compared to the first investigation. nutritive values(crude protein content, neutral detergent fiber content, acid detergent fiber content, digestibility) were good in pastures mixed with C1 Especially, nutritive values in pastures mixed with tall was higher than those of pastures mixed short grasses. P content among minerals in Treatment 1 was higher than other groups. However, the content of Ca, Mg and Mn were lower. The contents of Ca, K, Mg, Na, Cu, Zn and Fe in Treatment 2 were higher. However, the contents of K, Mg, Na, Cu, Zn and Fe in Treatment 3 were lower. Therefore, we suggest that cool season grasses with short grasses were sowed to establishing pasture suitable for grazing horses at an altitude of 400 m in Jeju island.