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http://dx.doi.org/10.1016/j.jgr.2018.04.005

Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice  

Jeong, Hyeon-Ju (Department of Molecular Cell Biology, Samsung Medical Center, Single Cell Network Research Center, Sungkyunkwan University School of Medicine)
So, Hyun-Kyung (Department of Molecular Cell Biology, Samsung Medical Center, Single Cell Network Research Center, Sungkyunkwan University School of Medicine)
Jo, Ayoung (Research Center for Cell Fate Control, Research Institute of Pharmaceutical Science, College of Pharmacy, Sookmyung Women's University)
Kim, Hye-Been (Department of Molecular Cell Biology, Samsung Medical Center, Single Cell Network Research Center, Sungkyunkwan University School of Medicine)
Lee, Sang-Jin (Research Center for Cell Fate Control, Research Institute of Pharmaceutical Science, College of Pharmacy, Sookmyung Women's University)
Bae, Gyu-Un (Research Center for Cell Fate Control, Research Institute of Pharmaceutical Science, College of Pharmacy, Sookmyung Women's University)
Kang, Jong-Sun (Department of Molecular Cell Biology, Samsung Medical Center, Single Cell Network Research Center, Sungkyunkwan University School of Medicine)
Publication Information
Journal of Ginseng Research / v.43, no.3, 2019 , pp. 475-481 More about this Journal
Abstract
Background: The ginsenoside Rg1 has been shown to exert various pharmacological activities with health benefits. Previously, we have reported that Rg1 promoted myogenic differentiation and myotube growth in C2C12 myoblasts. In this study, the in vivo effect of Rg1 on fiber-type composition and oxidative metabolism in skeletal muscle was examined. Methods: To examine the effect of Rg1 on skeletal muscle, 3-month-old mice were treated with Rg1 for 5 weeks. To assess muscle strength, grip strength tests were performed, and the lower hind limb muscles were harvested, followed by various detailed analysis, such as histological staining, immunoblotting, immunostaining, and real-time quantitative reverse transcription polymerase chain reaction. In addition, to verify the in vivo data, primary myoblasts isolated from mice were treated with Rg1, and the Rg1 effect on myotube growth was examined by immunoblotting and immunostaining analysis. Results: Rg1 treatment increased the expression of myosin heavy chain isoforms characteristic for both oxidative and glycolytic muscle fibers; increased myofiber sizes were accompanied by enhanced muscle strength. Rg1 treatment also enhanced oxidative muscle metabolism with elevated oxidative phosphorylation proteins. Furthermore, Rg1-treated muscles exhibited increased levels of anabolic S6 kinase signaling. Conclusion: Rg1 improves muscle functionality via enhancing muscle gene expression and oxidative muscle metabolism in mice.
Keywords
Atrophy; Exercise-specific signaling; Ginsenoside; Rg1; Skeletal muscle;
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1 Marabita M, Baraldo M, Solagna F, Ceelen JJM, Sartori R, Nolte H, Nemazanyy I, Pyronnet S, Kruger M, Pende M, et al. S6K1 is required for increasing skeletal muscle force during hypertrophy. Cell Rep 2016;17:501-13.   DOI
2 Holloszy JO. Biochemical adaptations in muscle. Effects of exercise in mitochondrial oxygen uptake and respiratory enzyme activity in skeletal muscle. J Biol Chem 1967;242:2278-82.   DOI
3 Gundersen K. Determination of muscle contractile properties: the importance of the nerve. Acta Physiol Scand 1998;162:333-41.   DOI
4 Willis LH, Slentz CA, Bateman LA, Shields AT, Piner LW, Bales CW, Houmard JA, Kraus WE. Effects of aerobic and/or resistance training on body mass and fat mass in overweight or obese adults. J Appl Physiol 2012;113:1831-7.   DOI
5 Wakahara T, Fukutani A, Kawakami Y, Yanai T. Nonuniform muscle hypertrophy: itsrelation to muscle activation in training session. Med Sci Sports Exerc 2013;45:2158-65.   DOI
6 Gallagher D, Belmonte D, Deurenberg P, Wang Z, Krasnow N, Pi-Sunyer FX, Heymsfield SB. Organ-tissue mass measurement allows modeling of REE and metabolically active tissue mass. Am J Physiol 1998;275:E249-58.
7 Czubryt MP, McAnally J, Fishman GI, Olson EN. Regulation of peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1 alpha) and mitochondrial function by MEF2 and HDAC5. Proc Natl Acad Sci USA 2003;100:1711-6.   DOI
8 Tadaishi M, Miura S, Kai Y, Kano Y, Oishi Y, Ezaki O. Skeletal muscle-specific expression of PGC-$1{\alpha}$-b, an exercise-responsive isoform, increases exercise capacity and peak oxygen uptake. PLoS One 2011;6: e28290.   DOI
9 Egan B, Zierath JR. Exercise metabolism and the molecular regulation of skeletal muscleadaptation. Cell Metab 2013;17:162-84.   DOI
10 Bodine SC, Stitt TN, Gonzalez M, Kline WO, Stover GL, Bauerlein R, Zlotchenko E, Scrimgeour A, Lawrence JC, Glass DJ, et al. Akt/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo. Nat Cell Biol 2001;3:1014-9.   DOI
11 Wang Y, Liu Q, Xu Y, Zhang Y, Lv Y, Tan Y, Jiang N, Cao G, Ma X, Wang J, et al. Ginsenoside Rg1 protects against oxidative stress-induced neuronal apoptosis through myosin IIA-actin related cytoskeletal reorganization. Int J Biol Sci 2016;12:1341-56.   DOI
12 Rommel C, Bodine SC, Clarke BA, Rossman R, Nunez L, Stitt TN, Yancopoulos GD, Glass DJ. Mediation of IGF-1-induced skeletal myotube hypertrophy by PI(3)K/Akt/mTOR andPI(3)K/Akt/GSK3 pathways. Nat Cell Biol 2001;3:1009-13.   DOI
13 Olson EJ, Pearce GL, Jones NP, Sprecher DL. Lipid effects of peroxisome proliferator-activated receptor-delta agonist GW501516 in subjects with low high-density lipoprotein cholesterol: characteristics of metabolic syndrome. Arterioscler Thromb Vasc Biol 2012;32:2289-94.   DOI
14 Narkar VA, Downes M, Yu RT, Embler E, Wang YX, Banayo E, Mihaylova MM, Nelson MC, Zou Y, Juguilon H, et al. AMPK and PPARdelta agonists are exercise mimetics. Cell 2008;134:405-15.   DOI
15 Go GY, Lee SJ, Jo A, Lee J, Seo DW, Kang JS, Kim SK, Kim SN, Kim YK, Bae GU. Ginsenoside Rg1 from Panax ginseng enhances myoblast differentiation and myotube growth. J Ginseng Res. 2017;41:608-14.   DOI
16 Huang HY, Korivi M, Hsu MF, Huang CY, Hou CW, Chen CY, Kao CL, Lee RP, Lee SD, Kuo CH. Oral Rg1 supplementation strengthens antioxidant defense system against exercise-induced oxidative stress in rat skeletal muscles. J Int Soc Sports Nutr 2012;9:23. 1-7.   DOI
17 Jeong HJ, Lee HJ, Vuong TA, Choi KS, Choi D, Koo SH, Cho SC, Cho H, Kang JS. Prmt7 deficiency causes reduced skeletal muscle oxidative metabolism and age-related obesity. Diabetes 2016;65:1868-82.   DOI
18 Bae GU, Lee JR, Kim BG, Han JW, Leem YE, Lee HJ, Ho SM, Hahn MJ, Kang JS. Cdo interacts with APPL1 and activates Akt in myoblast differentiation. Mol Biol Cell 2010;21:2399-411.   DOI
19 Leem YE, Jeong HJ, Kim HJ, Koh J, Kang K, Bae GU, Cho H, Kang JS. CDO regulates surface expression of Kir2.1 K+ channel in myoblast differentiation. PLoS One 2016;11: e015870.
20 Rolfe DF, Brown GC. Cellular energy utilization and molecular origin of standard metabolicrate in mammals. Physiol Rev 1997;77:731-58.   DOI
21 Zhang YJ, Zhang XL, Li MH, Iqbal J, Bourantas CV, Li JJ, Su XY, Muramatsu T, Tian NL, Chen SL. The ginsenoside Rg1 prevents transverse aortic constrictioninduced left ventricular hypertrophy and cardiac dysfunction by inhibiting fibrosis and enhancing angiogenesis. J Cardiovasc Pharmacol 2013;62:50-7.   DOI
22 Tang F, Lu M, Yu L, Wang Q, Mei M, Xu C, Han R, Hu J, Wang H, Zhang Y. Inhibition of $TNF-{\alpha}$-mediated $NF-{\kappa}B$ activation by ginsenoside Rg1 contributes the attenuation of cardiac hypertrophy induced by abdominal aorta coarctation. J Cardiovasc Pharmacol 2016;68:257-64.   DOI
23 Buller AJ, Eccles JC, Eccles RM. Interactions between motoneurons and muscles in respect of the characteristic speeds of their responses. J Physiol 1960;150:417-39.   DOI
24 Nunnari J, Suomalainen A. Mitochondria: in sickness and in health. Cell 2012;148:1145-59.   DOI
25 Magnuson B, Ekim B, Fingar DC. Regulation and function of ribosomal protein S6 kinase (S6K) within mTOR signalling networks. Biochem J 2012;441:1-21.   DOI
26 Lee H, Kim SH, Lee JS, Yang YH, Nam JM, Kim BW, Ko YG. Mitochondrial oxidative phosphorylation complexes exist in the sarcolemma of skeletal muscle. BMB Rep 2016;49:116-21.   DOI
27 Cannavino J, Brocca L, Sandri M, Bottinelli R, Pellegrino MA. $PGC1-{\alpha}$ overexpression prevents metabolic alterations and soleus muscle atrophy in hindlimb unloaded mice. J Physiol 2014;592:4575-89.   DOI
28 Li JB, Wassner SJ. Protein synthesis and degradation in skeletal muscle of chronically uremic rats. Kidney Int 1986;29:1136-43.   DOI