• Macromolecular Research
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• v.15 no.5
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• pp.424-429
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• 2007

A procedure for the surface hydrolysis of an electrospun poly(${\epsilon}$-caprolactone) (PCL) fibrous scaffold was developed to enhance the adhesion and proliferation of osteoblasts. The surface hydrolysis of fibrous scaffolds was performed using NaOH treatment for the formation of carboxyl groups on the fiber surfaces. The hydrolysis process did not induce deformation of the fibers, and the fibers retained their diameter. The cell seeding density on the NaOH-treated PCL fibrous scaffolds was more pronounced than on the non-treated PCL fibers used as a control. The alkaline phosphatase activity, osteocalcin and a mineralization assay strongly supported that the surface-hydrolyzed PCL fibrous scaffolds provided more favorable environments for the proliferation and functions of osteoblasts compared to the non-treated PCL fibrous scaffolds use as a control.

• Macromolecular Research
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• v.16 no.4
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• pp.345-352
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• 2008

Poly(lactic-co-glycolic acid) (PLGA) tubes (5 mm in diameter) were fabricated using an electro spinning method and used as a scaffold for artificial blood vessels through the hybridization of smooth muscle cells (SMCs) and endothelial cells (ECs) differentiated from canine bone marrow under previously reported conditions. The potential clinical applications of these artificial blood vessels were investigated using a canine model. From the results, the tubular-type PLGA scaffolds for artificial blood vessels showed good mechanical strength, and the dual-layered blood vessels showed acceptable hybridization behavior with ECs and SMCs. The artificial blood vessels were implanted and substituted for an artery in an adult dog over a 3-week period. The hybridized blood vessels showed neointimal formation with good patency. However, the control vessel (unhybridized vessel) was occluded during the early stages of implantation. These results suggest a shortcut for the development of small diameter, tubular-type, nanofiber blood vessels using a biodegradable material (PLGA).

• Prospectives of Industrial Chemistry
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• v.22 no.6
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• pp.26-40
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• 2019

고령화 사회에 접어들면서 신경, 정신질환으로 인한 사회경제적 부담이 늘어나고 있다. 이를 해결하기 위해서는 관련된 신경 회로를 직접적으로 자극하거나 그곳에서 일어나는 일을 실시간으로 감지할 수 있는 장비의 개발이 필수적이다. 하지만 이를 위한 수많은 공학적 도구의 개발에도 불구하고, 뛰어난 공간적/시간적 분해능, 세포형의 선택성, 장시간 안정성을 보유한 신경 인터페이스의 개발은 아직까지도 연구가 필요한 분야이다. 특히 신경전달 원리를 모두 이용하고자 하는 다기능 인터페이스의 개발은 최근 많은 연구자들의 관심 주제이고, 유연성을 가지는 인터페이스 개발 또한 안정성뿐만 아니라 신경 신호의 수명을 좌우하는 중요한 요소이기에 그 중요성을 인정받고 있다. 이를 해결하기 위한 여러 가지 과학적 시도 중에서도, 열 인장 공정으로 제작되는 섬유 형태의 장비는 그 통합적 기능을 수행하는 한 가지 방법으로써 많은 관심을 받고 있다. 이 기술은 다양한 기하학적 구조, 기능적 요소 등을 통합하는데 매우 유리하며, 또한 기존 반도체 공정으로 다루기 어려운 유연성 물질로 마이크로 스케일의 인터페이스를 제작하는 데에 매우 효과적이다. 본 기고문에서는 먼저 현재까지 개발되고 있는 다기능 유연 신경 인터페이스의 연구동향을 소개하고, 특히 그 중에서도 최근에 주목받고 있는 광섬유 기반의 인터페이스 개발에 대해 이야기하고자 한다.

• Journal of Periodontal and Implant Science
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• v.36 no.1
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• pp.27-37
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• 2006

Although the main purpose of periodontal treatment to regenerate is the complete regeneration of periodontal tissue due to periodontal disease, most of the treatment cannot meet such purpose because healing by long epithelial junction. Therefore, diverse materials of resorbable and non-resorbable have been used to regenerate the periodontal tissue. Due to high risk of exposure and necessity of secondary surgical procedure when using non-resorbable membrane, guided tissue regeneration using the resorbable membrane has gain popularity, recently. However, present resorbable membrane has the disadvantage of not having sufficient time to regenerate date to the difference of resorption rate according to surgical site. Meanwhile, other than the structure stability and facile manipulation, acellular dermal matrix has been reported to be a possible scaffold for cellular proliferation due to rapid revascularization and favorable physical properties for cellular attachment and proliferation. The purpose of this study is to estimate the influence of acellular dermal matrix on periodontal ligament, cementum and alveolar bone when acellular dermal matrix is implanted to 1-wall alveolar bone defect. 4 dogs of 12 to 16 month old irrelevant to sex , which below 15Kg of body weight, has been used in this study. ADM has been used for the material of guided tissue regeneration. The 3rd premolar of the lower jaw was extracted bilaterally and awaited for self-healing. subsequently buccal and lingual flap was elevated to form one wall intrabony defect with the depth and width of 4mm on the distal surface of 2nd premolar and the mesial surface of 4th premolar. After the removal of periodontal ligament by root planing. notch was formed on the basal position. Following the root surface treatment, while the control group had the flap sutured without any treatment on surgically induced intrabony defect. Following the root surface treatment, the flap of intrabony defect was sutured with the ADM inserted while the control group sutured without any insertion. The histologic specimen was observed after 4 and 8 weeks of treatment. The control group was partially regenerated by periodontal ligament, new cementum and new alveolar bone. the level of regeneration is not reached on the previous formed notch. but, experimental group was fully regenerated by functionally oriented periodontal ligament fiber. new cementum and new alveolar bone. In conclusion, we think that ADM seems to be used by scaffold for periodontal ligament cells and the matrix is expected to use on guided tissue regeneration.

• Journal of Adhesion and Interface
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• v.24 no.1
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• pp.17-25
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• 2023

In this study, waste polyethylene terephthalate (PET) was recycled to produce a support and then polyetherimide (PEI) was used for environmentally friendly organic solvent nanofiltration. The prepared composite membrane was first prepared by electrospinning a PET support, then casted on the support using PEI having excellent solvent resistance, and organic solvent nanoparticles using a Non-solvent Induced Phase Separation (NIPS) method. A filtration membrane was prepared. First, the fiber diameter and tensile strength of the PET scaffold prepared prior to membrane fabrication were identified through morphology analysis, and the optimal scaffold for the organic solvent nanofiltration membrane was identified. Afterward, the PET/PEI composite membrane prepared was checked for the DEA removal rate of Congo red having a molecular weight of 697 g/mol in ethanol to understand the performance as an organic solvent nanofiltration membrane according to the concentration of PEI. Finally, the removal rate of Congo red was 90% or more.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.277.2-277.2
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• 2013

Petri dishes and glass slides have been widely used as general substrates for in vitro mammalian cell cultures due to their culture viability, optical transparency, experimental convenience, and relatively low cost. Despite the aforementioned benefit, however, the flat two-dimensional substrates exhibit limited capability in terms of realistically mimicking cellular polarization, intercellular interaction, and differentiation in the non-physiological culture environment. Here, we report a protocol of culturing embryonic rat hippocampal neurons on the electro-spun polymeric network and the results from examination of neuronal cell behavior and network formation on this culture platform. A combinatorial method of laser-scanning confocal fluorescence microscopy and live-cell imaging technique was employed to track axonal outgrowth and synaptic connectivity of the neuronal cells deposited on this model culture environment. The present microfiber-based scaffold supports the prolonged viability of three-dimensionally-formed neuronal networks and their microscopic geometric parameters (i.e., microfiber diameter) strongly influence the axonal outgrowth and synaptic connection pattern. These results implies that electro-spun fiber scaffolds with fine control over surface chemistry and nano/microscopic geometry may be used as an economic and general platform for three-dimensional mammalian culture systems, particularly, neuronal lineage and other network forming cell lines.