• 제목/요약/키워드: fetilization

검색결과 7건 처리시간 0.015초

가토난자의 체외수정과 체외배양 (In vitro Fertilization and Culture of Rabbit Egg)

  • 박흠대;이경광;정길생
    • 한국가축번식학회지
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    • 제4권1호
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    • pp.75-82
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    • 1980
  • This experiment was carried out to improve a simple and effective procedure of egg transfer which is considered to be the most useful technique for the improvementand proliferation of domestic animals. Several experiment procedures such as superovulation, surgical recovery of ovulated egg, in vitro capacitation of ejaculated spermatozoa, in vitro fertilization and culture of embryo were conducted. The results obtained in this experiment were summarized as follows: 1. In rabbits treated with PMS in combination with estradiol and HCG, 10 to 43 eggs were obtained in one rabbit and the average ovulation number of 20 rabbits was 21 eggs. 2. Six to 24 eggs (average 13 eggs) were recovered from the removed oviducts by the methods of flushing technique and the average recovery rate of 20 rabbits was 61.9 percent. 3. Most rabbit spermatozoa ejaculated by artificial vagina were capacitated in vitro by the culture of the spermatozoa with PBI medium at 37$^{\circ}C$ for 4 hours after removal of seminal plasma. 4. Normal fetilization following in vitro fetilization was observed in 88 (42.7%) of 206 eggs. 5. The number of eggs developed to 2-, 4-, and 8-cell stage following in vitro culture with PBI medium at 37$^{\circ}C$ was 26 (70.3%), 20 (54.1%) and 17 (45.9%) of 37 eggs used for in vitro culture.

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버어리종 건조방법에 따른 질소화합물의 변화 (Changes of Nitrogeneous Compounds Depending upon the Curing Methods in Burley Tobacco)

  • 백순옥;한상빈;배성국
    • 한국연초학회지
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    • 제25권1호
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    • pp.27-33
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    • 2003
  • This study was conducted to determine the changes of nitrogen compounds in lamina and midrib during the curing process with the different curing methods, such as priming and stalk-cut curing. After KB 108 burley tobacco was cultivated by the different fetilization levels such as standard and 20% higher, only the tips and leaf were harvested. Though the contents of total alkaloid and total nitrogen were similar in lamina, midrib showed a very low contents of those components by the different curing method and fertilization levels. The content of nitrate-nitrogen in lamina increased during a middle of curing process, and then these compound was decreased during an end of curing process by stalk-cut curing method. Nitrate-nitrogen contents in the lamina by the priming curing showed a high level caused by rapid drying process during an end of curing process. That component in midrib was 5-6 times higher than that of lamina. The contents of ammonia-nitrogen in the lamina and midrib were increased during a curing process. Though those amount in tips showed a similar by a different fetilization and curing method, midrib of stalk-cut curing showed a slow increasing during a curing process.

돼지 체외수정의 현황과 문제점 (The Present Situation and Problems of In Vitro Fertilization in Swine)

  • 류일선
    • 한국수정란이식학회지
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    • 제7권1호
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    • pp.41-47
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    • 1992
  • 1. In vitro system, LR and FSR accelerated and facilitated meiotic progression, and LH selectively improved cytoplasmic maturation which is required to promote the formation of a male pronucleus. 2. Caffeine (2mM) in the fetilization medium was required not only for inducing zona penetrating ability of boar also for developing to the male pronucleus of the penetrat- ing spermatozoa in vitro. 3. The germinal vesicle (GV)stage was observed for the first 17.6 hr;germinal vesicle break-down (GVBD)stage between 17.6~26.4 hr ;metaphase I (M-I)from 26.4 - 30. 9hr;anaphase I(A-I)ranged from 30. 9~33.4hr;telophase I(T-I) at 33.4~34.4hr; and metaphase II(M-II) at 34.4-48hr. 4. The addition of 10%(v /v) pig follicular fluid (pFF) to maturation media significantly increased the rate of nuclear maturation of pig oocytes (p<0.01), whereas the rate of nuclear maturation of pig oocytes among three different media did not differ. 5. The presence of a primary culture of POEC promotes in vitro development of early cleavage stage pig embryos.

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Response of a Miscanthus sinensis Grassland in an Early Successional Old-Field to Fertilization

  • Song, Jong-Suk
    • Journal of Plant Biology
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    • 제37권1호
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    • pp.1-8
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    • 1994
  • The effects of fetilization on the structure and function of an early successional Miscanthus sinensis grassland were investigated in an old-field ecosystem at Honjo city, Saitama Prefecture, Japan from 1981 to 1982. Compared to control plot, life form composition of treatment plot was characterized by a decrease of phanerophytes and an increase of hemicryptophytes. Fertilization had a beneficial effect on the growth in aboveground phytomass of M. sinensis, the dominant species. However, it did not cause a change in the total number of stems of M. sinensis. Annual increment of patch diameter of M. sinensis was estimated to be 6-8 cm on an average and there was no significant differecne in the growth rate of patch size between fertilized and nonfertilized plots. To estimate the aboveground phytomass of M. sinensis, several non-destructive parameters were examined. As a result, the patch size showed a high correlation with aboveground phytomass. Thus the patch size was suggested to be most applicable to its estimation. Diversity indices based on phytomass data of component species were increased slightly by fertilizatin, because relative dominance of some other species, especially of Artemisia princeps increased, while that of M. sinensis decreased. Fertilizer application resulted in a decrease in the total number of species.

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비료의 성분 및 종류와 묘목과의 관계 연구 II. 묘목의 부위별 양분 농도에 미치는 영향 (Relationship Between Composition and Type of Fertilizer and Seedling Growth II. Nutrient Concentration of Seedling Components)

  • 황정옥;손요환;이명종;변재경;정진현;이천용
    • 임산에너지
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    • 제24권1호
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    • pp.13-27
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    • 2005
  • 새로운 산림용 복합비료를 개발하기 위하여 소나무, 낙엽송, 자작나무, 상수리나무 묘목을 대상으로 성분비가 다른 비료 및 종류가 다른 고형복합비료를 처리하고 묘목의 부위별 양분 농도를 측정하였다. 질소, 인, 칼륨의 비를 각기 달리한 시비수준별 처리실험에서 질소와 인 시비 후 묘목내 이들 양분의 농도가 증가하였다. 또한 소나무와 상수리나무에서 칼륨 시비량 증가에 따라 묘목 내 양분 농도가 증가하는 효과를 보였으나, 나머지 두 수종에서는 처리별 차이가 나타나지 않았다. 산림용 고형복합비료와 UF 고형복합비료를 처리한 비료 종류별 처리실험에서는 시비량이 증가할수록 묘목 내 질소와 인의 농도가 증가하는 현상이 나타났다. 소나무에서의 칼륨 농도와 낙엽송에서의 나트륨과 칼슘 농도도 유사한 현상을 보였으며, 나머지 수종에서는 처리별 차이를 나타내지 않았다. 비료의 종류를 달리하여 처리한 결과 묘목 내 양분 농도 변화 효과는 없는 것으로 나타났다.

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질소시비수준과 예취관리가 수단그라스계 잡종 [ Sarghum Bicolor ( L. ) Moench ] 의 저장탄수화물함량 , 재생 및 수량에 미치는 영향 I. 질소시비수준과 예취높이가 수단그라스계 잡종의 예취후 신지의 발생 , 건물량 및 고사에 미치는 영향 (Effect of Nitrogen Fertilization and cutting management on the Carbohydrate Reserves, Regrowth, and Dry Matter Yeild of Sorghum-Sudangrass Hybrid[Sorghum bicolor(L.)Moench]for Forage Production I. Effect of nitrogen fetilization and cutting height on the appearance, dry weight and death of new bud after cutting of sorghum-sudangrass hybrid)

  • 서성;김동암
    • 한국초지조사료학회지
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    • 제3권2호
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    • pp.58-66
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    • 1983
  • 본(本) 시험(試驗)은 여름철 청예사초(靑刈飼草)로서 이용(利用)이 급증하고 있는 수단그라스계(系) 잡종(雜種) [수수-수단그라스잡종(雜種); Sorghum bicolor (L.) Moench] 중 우리나라에서 생산성(生産性)과 적응성(適應性)이 가장 우수(優秀)하다고 인정된 Pioneer 988을 공시품종(供試品種)으로 하여 질소시비수준(窒素施肥水準)과 예취(刈取)높이가 신지(新枝)(측지(側枝) 및 분얼경(分蘖莖))의 발생(發生), 건물중(乾物重) 및 고사(枯死)에 미치는 영향을 구명(究明)하기 위하여 1981년(年)과 1982년(年) 2년(年)에 걸쳐 서울대(大) 농대(農大) 부속실험목장내 사초시험포(飼草試驗圃)에서 실시(實施)되었으며 결과(結果)를 요약(要約)하면 다음과 같다. 1. 질소시비수준(窒素施肥水準)은 신지(新枝)의 발생(發生)이나 고사(枯死)에 별 영향을 주지 못하였다. 2. 예취후(刈取后) 신지(新枝)에 있어서 예취(刈取)높이가 낮아질록 측지수(側枝數)는 감소하고 분얼경수(分蘖莖數)는 증가하였다. 3. 재새수량(再生收量)에서 측지(側枝)가 차지하는 비율은 작으며 예취(刈取)높이에 관계없이 대부분의 수량(收量)은 분얼경(分蘖莖)이 차지하였다. 4. 신지(新枝)의 고사(枯死)는 예취(刈取)높이가 낮은 구(區)에서 증가하였다.

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서울시내 실업계 여고생들의 성지식 태도 및 행위에 관한 조사연구 (A Study on the Knowledge. Attitude and Behavior of Commercial Girl's High School Students Toward Sex)

  • 배남숙
    • 보건교육건강증진학회지
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    • 제1권1호
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    • pp.57-71
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    • 1983
  • Sex education is necessary for the youth that they should have an adequate sexual knowledge, attitudes and behaviors in their adolescent period. Four major objectives of this study are as follows; 1. To know the level of sexual knowledge of commercial girl's high school students in Seoul. 2. To know their actual state for the attitudes and behaviors toward sex. 3. To compare the sexual knowledge, attitudes and behaviors of day time school students with those of night time school students. 4. To compare the factors associated with their sexual knowledge, attitudes and behaviors with the individual level. Data were collected on 986 students in Seoul from April 1 to April 10, 1983. As the result of this survey, the following conclusions were obtained. 1. The level of sexual knowledge. (1) The level of knowledge of day time high school students about the physiology of female and pregnancy is shown higher than those of night time students. 64.2% of the respondents are aware of the organ producing ovum. 56.4% the ovulation period. 95.6% the cause of pregnancy. 74.5% the pregnantable period and 12.7% the place of fetilization. (2) Out of 986 respondents. 71.8% knew about contraceptive method correctly, and day time school students knew litter better than night time school students, by showing 73.9% and 69.7% respectively. They knew about contraceptive method with 'oral pills'. 'menstrual cycles', 'condoms'. and 'loops' in the same order. 3) Kinds of veneral disease was correctly known by 37.9%. Day time students knew much better than night time school students. by showing 67.8% and 7.9%. respectively. Transmission method of veneral disease was correctly known by 28.3%. Day time students knew much better than night time students, by showing 51.2% and 5.3%, respectively. (4) The major information source of sexual knowledge was 'book and magazine' (39.9%) and 'friends' (27.4%). 2. Actual state of attitudes and behaviors toward sex. (1) Out of 986 respondents, 84.0% answered that premarital purity should be kept. (2) Out of 986 respondents, 60.8% had an acquaintance of the opposite sex. 45.2% of students with opposite sex reported introduction of their friends as the main channel of making an acquaintance of the opposite sex. (3) Of those who responded to this study 13.8% reported having masturbation, 21.5% kissing, 6.2% petting and 3.7% sex intercourse. (4) 64.8% had sexual problems, which was mensturance (27.2%), aquaintance of the opposite sex (25.4%). The main method to solve the problems were consultation with 'friends' and 'books and magazine' percentage being 39.1% and 30.8%, respectively, whereas very small students discussed with 'teachers' and 'parents' percentages being 0.3% and 5.9%, respectively. (5) Out of 986 respondents, 62.2% had experience in educating about sex, the percentage of day time school was higher than that of night time school. (6) 88.2% of students wanted sex education in school, which were 'general information about sex' (35.4%), 'sexual morality and solution of sexual desire' (18.5%), 'aquaintance of the opposite sex' (13.3%) and 'marriage and role of man and woman' (12.4%) in the same order. They wanted to be instructed about sexes in the lecture of physical education, home economics, biology, military training (59.9%), regular curriculum (17.5%), special lecture (16.9%) and by the counselor or school nurse (5.7%). 3. Analyse concerning the factors about the knowledge, attitudes and behaviors. (1) The better school record was, the higher the level of sexual knowledge. (2) Those who have the religion considered the permarital purity more important than those who have not, the percentage showing 86.7% and 80.7%, respectively. (3) The result of dyad analysis of making acquaintance of the opposite sex in the friendship network showed that a high index of the acquaintance of the opposite sex tends to be a high adoption of making one at the individual level in the group, while the low index tends to be a low adoption of making one in the group.

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