• Journal of The Korean Society of Grassland and Forage Science
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• v.8 no.1
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• pp.40-46
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• 1988

This study was carried out to determine the effects of nitrogen (N) fertilization levels (0, 100 and 200kg/ha) and formaldehyde (0.4,0.8 and 1.2%: w/w CM) on the chemical composition, dry matter (DM) yield and in-vitro dry matter digestibility (IVDMD) of forage rape (Brassica napus Subsp. oleifera) silage. The results obtained are summarized as follows : 1. Forage rape was a catch crop which was highly digestible and low concentration in NDF, ADF, cellulose and lignin. 2. The concentration of the water soluble carbohydrate (WSC) and buffering capacity (PK) was 17.9% and 6.77g/100g, respectively, provided the WSC to PK ratio was 2.65. 3. DM content of silage decreased as the rate of N fertilization increased, but concentration of ammonia-N decreased. IVDMD was not affected by the rate of N fertilization. 4. By the addition of formaldehyde, the pH and IVDMD were increased, but the DM, lactic acid, total organic acid and ammonia-N content of rape silage were decreased, that was effective as a silage additive for forage rape.

• Journal of the Korea Organic Resources Recycling Association
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• v.22 no.4
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• pp.28-44
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• 2014

Due to development of the national economy growth, livestock goods consumption has rapidly increased over the past 30 years. It has led livestock breeders to increase their livestock numbers. An increased number of livestock have consequently resulted in an increasing animal feces generation. According to the agricultural statistics provided by the Bureau of Statistics, livestock manure amounts to 47,235 thousand tons in 2013. To treat livestock manure, various types of treatment facilities like composting, liquid fertilization, purification, and anaerobic digestion facilities are being applied. In composting facility, there are four kinds of agitation system: escalator, paddle, screw and rotary type. In case of liquid fertilization process, there are two types of system: aeration and anoxic type. There are about 8,000 liquid fertilization facilities for treatment livestock manure in Korea. For purification of livestock manure, the treatment process is divided by three steps: Solid/Liquid separation process, Secondary purification process and advanced oxidation process. About 21 thousand tons of livestock manure was treated by anaerobic digestion facility in 2012. In every type of facility for livestock manure treatment, it is very important to choose the optimal deodorization equipment for the livestock manure treatment facility. In this study, the investigation has been carried out for six years to analyse the characteristics of livestock manure treatment facilities and related technique of Korea.

• The Journal of Korean Obstetrics and Gynecology
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• v.27 no.4
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• pp.141-149
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• 2014

Objectives: The purpose of this study is to report the effects of herbal medicine and acupuncture on ovarian hyperstimulation syndrome (OHSS). Methods: A patient who had OHSS with edema and pain of left leg as chief complaints was treated by herbal medicne and acupuncture for 11days. We evaluated the results of treatment by change of symptoms, circumference of left leg, Visual Analogue Scale about left leg pain and walking time. Results: After treatment, symptoms of OHSS were almost improved. Circumference and pain of left leg were reduced and walking time was incresed after treatment. Conclusions: This study suggests that Korean medicine mainly herbal medicine and acupuncture is effective on OHSS.

• Journal of Bio-Environment Control
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• v.28 no.1
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• pp.66-77
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• 2019

The present study investigated pine trees, which forms a major plantation species in Korea, with the objective of improving the survival rate of pine trees after planting. Growth responses and characteristics were assessed by controlling the level of fertilizer application, which is a basic controlling the growth of pine seedlings, to identify the optimal fertilization treatment. Pine tree seedlings were grown in 104 containers and were examined 8 weeks after planting. Stem height and were measured at 4-week intervals. In terms of fertilization treatment for 1-0 pine seedlings, the treatment group with gradually-increasing fertilizer concentration ($500{\rightarrow}1000{\rightarrow}1000{\rightarrow}1000mg{\cdot}L^{-1}$) had the biggest increase in stem height and diameter at the root. The survey results indicated that the increased concentration treatment group and the gradually-increasing concentration treatment group had more growth compared with that in the fixed concentration treatment group. The gradually-increasing concentration treatment group ($500{\rightarrow}1000{\rightarrow}1000{\rightarrow}1000mg{\cdot}L^{-1}$) had the highest total dry matter production. Nine weeks after fertilization, the tips of the pine leaves turned yellow in the fixed concentration treatment group ($3000mg{\cdot}L^{-1}$). The same phenomenon was observed in the treatment group in which the concentration was increased to $2000mg{\cdot}L^{-1}$, and in the gradually-increasing concentration treatment group, when the concentration was raised up to $2000mg{\cdot}L^{-1}$. We concluded that the optimal fertilization conditions for producing healthy pine 1-0 seedlings involve fertilizing once a week with Multifeed 19 at $500mg{\cdot}L^{-1}$ during the seedling period, Multifeed 19 at $1000mg{\cdot}L^{-1}$ during the rapid growth period, and Multifeed 32 at $1000mg{\cdot}L^{-1}$ during the maturation period.

• Journal of Embryo Transfer
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• v.28 no.1
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• pp.41-48
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• 2013

This study examined the motility of either the unattached(upper) or attached(lower) Hanwoo sperm to bovine oviduct epithelial cell(BOEC) monolayers to determine whether there are any changes in their motility during co-culture. The cleavage and blastocyst development rate were compared among different preincubation methods in-vitro, after oocytes were fertilized in-vitro with Hanwoo sperm on BOEC monolayers. The motility of frozen-thawed sperm in BOEC co-culture group was significantly higher than controls, especially at 5 hours and 6 hours (p<0.05) of incubation, in sperm treatment medium without heparin and caffeine. The motility of frozen-thawed sperm in BOEC co-culture group was significantly higher than controls, especially at 3 hours (p<0.05) and 6 hours (p<0.01), in sperm treatment medium containing heparin and caffeine. The motility of the attached( lower) sperm was significantly higher than the unattached(upper) sperm during co-culture with BOEC at all times(p<0.01 or p<0.05), except for 6 hours. After Hanwoo oocytes were fertilized in-vitro with the sperm that had been co-cultured with BOEC in sperm treatment medium containing heparin and caffeine, we determined the cleavage and blastocyst development rate, according to the preincubation methods. Both the cleavage and blastocyst development rate from 2 hour preincubation group were the highest, but significant difference was not recognized. These results show that BOEC plays an important role on sperm hyperactivation related to capacitation regardless of heparin and caffeine in sperm treatment medium. However, oviduct epithelial cell had no significant effect on the development of embryos after in-vitro fertilization in the presence of added heparin and caffeine in sperm treatment medium.

• Clinical and Experimental Reproductive Medicine
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• v.19 no.1
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• pp.71-79
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• 1992

In vitro fertilization and embryo transfer (IVF & ET) is widely used for the males with subnormal or abnormal semen quality, as this was recommended in view of the relatively small numbers of spermatozoa required for fertilization and subsequent pregnancies could be obtained. The aim of this study is to know how the various functional parameters of spermatozoa in semen analysis affect the outcome IVF. This study was carried out between 1988-1989, with male factor patients selected on the basis of the semen quality. The selection criteria was based upon the mean values of concentration,% motility and % normal morphology from at least two semen analysis. There is a significant decrease in the fertilization and embryo transfer rates in the study group compared with control group (35.9% vs. 68% and 48.6% vs. 85.5% respectively), however, there was no significant difference in the pregnancy or delivery rates (19.6% vs. 21.4% and 60.0% vs. 62.5% respectively) per embryo transfer cycles. Fertilization rate is variously affected by the type and degree of sperm defect. No pregnancy was occurred in triple defect group and asthenoteratospermia group. There is no significant increase in the abortion rate in the male factor group. Improvement have to be made with the fertilization rate, as the pregnancy rate per OPU cycle in male factor group is still lower than that of normal group (9.5% vs. 18.3%). In conclusion, IVF can be used as a treatment for male factor infertility and the preparation of the semen sample can be modified to improve sperm recovery and obtain fertilization from abnormal semen samples.

• Korean Journal of Agricultural Science
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• v.45 no.1
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• pp.9-18
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• 2018

This study was done to determine the effect of additional nitrogen fertilization on the yield and quality of the Korean wheat cultivars Keumkang, Jokyoung, Baegjoong, Sooan, Uri and Goso. Different levels of nitrogen applications (109, 82, 55, 41, and 27 kg/ha) were applied to six cultivars. The results show that the yield and protein contents were increased in all tested cultivars. The grain yields of the cultivars Keumkang, Jokyoung, Baegjoong and Sooan were greatly increased in the case of double fertilization treatments. Moreover, Uri and Goso had greatly increased yields by the additional fertilization at a 50% rate compared with korea wheat standard fertilization rate. A significantly higher yield was observed in Uri. Baegjoong was the highest yielding cultivar among the tested cultivars with the additional nitrogen fertilization. As the fertilization was increased up to double the fertilization treatment, the yield of Baegjoong also showed a constant increase. Positive correlations were found between the nitrogen fertilizer application levels and the protein contents of the grain in all the cultivars except for Uri, and among these, Jokyoung had a most significant correlation between the nitrogen fertilizer application level and the increase in its protein contents. Keumkang had the highest protein contents and highest increase in the protein content change according to the amount of nitrogen application. However, amylose, damaged starch and ash contents were not significantly changed by the different levels of nitrogen applications.

• Clinical and Experimental Reproductive Medicine
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• v.44 no.1
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• pp.15-21
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• 2017

Objective: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of $Ca^{2+}$ chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). Methods: MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a $Ca^{2+}$ chelator to investigate the effect of $Ca^{2+}$ oscillations on their maturation. Results: As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the $Ca^{2+}$ chelator-treated group. Conclusion: The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by $Ca^{2+}$ chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular $Ca^{2+}$ oscillations driven by fertilization.

• Journal of Korean Academy of Nursing
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• v.42 no.3
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• pp.369-376
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• 2012

Purpose: The purpose of this study was to identify the effects of a Yoga-focused prenatal program on the stress, anxiety, self confidence and labor pain of pregnant women who had in vitro fertilization (IVF) treatment. Methods: A quasi experimental study with a non-equivalent control group pretest-posttest design was used. The data collection period and meditation program were between January 9 and August 31, 2009. Forty-six women who were pregnant following IVF, and were between 12-20 weeks gestation, participated in the study (23 experimental group, 23 control group). Data were analyzed using Chisquare test, Mann-Whitney U Test, ANCOVA, and Cronbach's alpha coefficients with the SPSS 12.0 for Windows Program. Results: Although the sample size was limited, women who participated in the program showed statistically significant improvements in stress, anxiety, labor pain, and labor confidence for women pregnant after IVF. Conclusion: The result indicate that this 12-week Yoga-focused educational program can be utilized for women pregnant following IVF to reduce their stress, anxiety, and labor pain, and to increase delivery confidence. It is suggested that the Yoga-focused educational program be offered to every pregnant woman.

• Korean Journal of Animal Reproduction
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• v.13 no.1
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• pp.26-31
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• 1989

This study was carried out to investigate the effects of PMSG and/or HCG treatment on superovulation and fertilization in the golden hamster. The results obtained were summarized as follows: 1. The female groups treated with 30 IU PMSG or 30 IU PMSG＋25IU HCG ovulated more eggs than those treated with 15 IU PMSG or 15 IU PMSG＋25IU HCG(P<.01). All the PMSG treatment groups superovulated as compared with the untreated control group(P<.01). There were no differences on fertilization rate between the superovulated groups and the control group. 2. The fertilized ova were obtained only by the female group treated with 30 IU PMSG at 1000hr on day 1(morning of ovulation) of the estrous cycle. 3. The intervals between PMSG and HCG injection necessary to obtain the consistent superovulation and fertilized ova were 66hr and 72hr. 4. The superovulated ova were collected from oviduct 48hr, oviduct and uterus 72hr, and uterus 96 hr after mating. 80.3% of two cell, 75.8% of eight cell, and 73.7% of blastocyst of the ovulated ova occurred 48hr, 72hr, and 96hr after mating, respectively.