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http://dx.doi.org/10.12750/JET.2013.28.1.41

The Motility of Bovine Sperm and Fertilization Rate of Oocytes during in-vitro Fertilization following Oviduct Epithelial Cell Co-culture  

Lee, Hae-Lee (Livestock Experiment Station, Jeollabuk-do Institute of Livestock and Veterinary Research)
Park, Jae-Hee (Livestock Experiment Station, Jeollabuk-do Institute of Livestock and Veterinary Research)
Kim, Yong-Su (Livestock Experiment Station, Jeollabuk-do Institute of Livestock and Veterinary Research)
Kim, Jong Gug (Department of Animal Science and Institute of Rare Earth for Biological Application, College of Agriculture and Life Science, Chonbuk National University)
Publication Information
Journal of Embryo Transfer / v.28, no.1, 2013 , pp. 41-48 More about this Journal
Abstract
This study examined the motility of either the unattached(upper) or attached(lower) Hanwoo sperm to bovine oviduct epithelial cell(BOEC) monolayers to determine whether there are any changes in their motility during co-culture. The cleavage and blastocyst development rate were compared among different preincubation methods in-vitro, after oocytes were fertilized in-vitro with Hanwoo sperm on BOEC monolayers. The motility of frozen-thawed sperm in BOEC co-culture group was significantly higher than controls, especially at 5 hours and 6 hours (p<0.05) of incubation, in sperm treatment medium without heparin and caffeine. The motility of frozen-thawed sperm in BOEC co-culture group was significantly higher than controls, especially at 3 hours (p<0.05) and 6 hours (p<0.01), in sperm treatment medium containing heparin and caffeine. The motility of the attached( lower) sperm was significantly higher than the unattached(upper) sperm during co-culture with BOEC at all times(p<0.01 or p<0.05), except for 6 hours. After Hanwoo oocytes were fertilized in-vitro with the sperm that had been co-cultured with BOEC in sperm treatment medium containing heparin and caffeine, we determined the cleavage and blastocyst development rate, according to the preincubation methods. Both the cleavage and blastocyst development rate from 2 hour preincubation group were the highest, but significant difference was not recognized. These results show that BOEC plays an important role on sperm hyperactivation related to capacitation regardless of heparin and caffeine in sperm treatment medium. However, oviduct epithelial cell had no significant effect on the development of embryos after in-vitro fertilization in the presence of added heparin and caffeine in sperm treatment medium.
Keywords
motility; oviduct epithelial cell; hyperactivation; capacitation;
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