• 한국자원식물학회지
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• 제9권2호
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• pp.165-170
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• 1996

산채(山菜) 및 약용(藥用)으로서의 이용개발(利用開發) 가능성(可能性)이 높은 물엉겅퀴에 대(對)한 엽형(葉形) 특성(特性)을 조사(調査)하고, 차광(遮光) 조건(條件), 시비방법(施肥方法) 및 재식밀도(栽植密度) 시험(試驗)을 실시(實施)하여 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 1. 엽형(葉形)의 차리(差異)에 따라 크게 무결각형(無缺刻形)과 결각형(缺刻形)으로 나눌 수 있었고 무결각(無缺刻) 형(形)에서는 대자침(大刺針)과 소자침(小刺針)으로 세분(細分)되었으며 염색체(染色體) 수(數)는 공(供)히 2n = 68로 동일(同一)하였다. 2. 물엉겅퀴의 지상부(地上部) 초장(草長)은 무차광(無遮光)에서 길었으며 비료(肥料) 시용방법간(施用方法間)에 전반적(全般的)으로 유기물(有機物) 30,000kg/ha 처리(處理)에서 생육(生育)이 양호(良好)한 경향(傾向)이었다. 3. 차광(遮光) 조건간(條件間)의 엽(葉) 수량(收量)은 연차별(年次別) 차이(差異)는 차광구(遮光區)에서 많았으며 비료시용방법간(肥料施用方法間)에는 유기물(有機物) 처리(處理)에서 증대(增大)되는 경향(傾向)을 보였다. 4. 물엉겅퀴의 재식거리(栽植距離)에 따른 생육상황(生育狀況)은 $60\times45cm$에서 초장(草長)과 경수(莖數)가 증대(增大)되었다. 5. 재식거리별(栽植距離別) 엽수량(葉收量)은 밀식(密植)인 $30\times20cm$에서 많아 재식거리(栽植距離)와 수량간(收量間)의 관계(關係)에 대(對)한 구체적(具體的)인 연구(硏究)가 금후(今後) 수행(遂行)되어야 할 것으로 사료(思料)되었다.

• Clinical and Experimental Reproductive Medicine
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• 제43권3호
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• pp.169-173
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• 2016

Objective: To determine the preferred regimen for women with adenomyosis undergoing in vitro fertilization (IVF), we compared the IVF outcomes of fresh embryo transfer (ET) cycles with or without gonadotropin-releasing hormone (GnRH) agonist pretreatment and of frozenthawed embryo transfer (FET) cycles following GnRH agonist treatment. Methods: This retrospective study included 241 IVF cycles of women with adenomyosis from January 2006 to January 2012. Fresh ET cycles without (147 cycles, group A) or with (105 cycles, group B) GnRH agonist pretreatment, and FET cycles following GnRH agonist treatment (43 cycles, group C) were compared. Adenomyosis was identified by using transvaginal ultrasound at the initial workup and classified into focal and diffuse types. The IVF outcomes were also subanalyzed according to the adenomyotic region. Results: GnRH agonist pretreatment increased the stimulation duration ($11.5{\pm}2.1days$ vs. $9.9{\pm}2.0days$) and total dose of gonadotropin ($3,421{\pm}1,141IU$ vs. $2,588{\pm}1,192IU$), which resulted in a significantly higher number of retrieved oocytes ($10.0{\pm}8.2$ vs. $7.9{\pm}6.8$, p=0.013) in group B than in group A. Controlled ovarian stimulation for freezing resulted in a significantly higher number of retrieved oocytes ($14.3{\pm}9.2$ vs. $10.0{\pm}8.2$, p=0.022) with a lower dose of gonadotropin ($2,974{\pm}1,112IU$ vs. $3,421{\pm}1,141IU$, p=0.037) in group C than in group B. The clinical pregnancy rate in group C (39.5%) tended to be higher than those in groups B (30.5%) and A (25.2%) but without a significant difference. Conclusion: FET following GnRH agonist pretreatment tended to increase the pregnancy rate in patients with adenomyosis. Further largescale prospective studies are required to confirm this result.

• 한국산림과학회지
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• 제103권4호
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• pp.703-708
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• 2014

본 연구는 몽골 반건조 지역 현지에서 토양 개량이 묘목의 활착 및 생장에 미치는 효과를 알아보기 위해 수행되었다. 이를 위하여 알칼리성 사질 토양인 연구대상지에 2년생 백양나무(Populus sibirica)와 비술나무(Ulmus pumila) 묘목을 심고 질소(시비량에 따라 2수준), 황, 인공보습제, 황토가공소재 등을 처리하였다. 4개월 뒤, 두 수종의 묘목 활착률은 질소 시비량의 증가에 따라 감소하였다. 일반적으로 질소의 시비는 양분을 공급하여 묘목의 활착률 및 생장을 높이는 것으로 알려져 있다. 그러나 본 연구 결과에서 나타난 활착률 감소는 과다시비로 인한 토양 삼투압의 증가에 의한 것으로 보인다. 두 수종 모두 황토가공물질 처리구에서 근원경 생장에 유의한 증가가 나타났다. 그리고 백양나무의 경우 인공보습제 처리구에서도 근원경 생장이 유의하게 증가하였는데, 이는 백양나무가 건조에 내성이 있음에도 불구하고 수분 요구도가 높은 포플러속에 속하기 때문인 것으로 보인다. 반면 황 처리는 두 수종의 활착률과 근원경 생장 모두에서 효과가 없는 경향이 나타났는데, 이는 건조지 토양의 낮은 황산화율 때문으로 추정된다. 향후 건조/반건조 지역에서 토양 개량의 장기적인 효과를 알아보기 위하여 묘목 생장 및 토양 특성에 대한 지속적인 모니터링이 요구되며, 연구 수종에 적합한 시비량 산정을 위한 추가 연구가 필요한 것으로 판단된다.

• 아시안잔디학회지
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• 제25권2호
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• pp.217-222
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• 2011

최근 잔디관리를 위한 시비프로그램에서 인산의 사용량을 줄이기 위한 연구가 많이 진행 되고 있다. 토양의 인산함량이 식물성장을 위해서 충분히 존재하더라도 시비프로그램은 질소의 양을 기준으로 이루어져 있기 때문에 인산이 불필요 하더라도 질소와 같이 시비되는 것이 일반적이다. 많은 선행연구에서 이미 조성된 잔디에서는 토양내 인산함량의 조건에 상관없이 인산의 영향이 발견되지 않았다. 이것은 뗏장으로 조성된 켄터키블루그래스의 대취층에 많은 인산의 함량이 발견되었기 때문이다. 본 연구에서는 대취층을 최소화 하기위하여 파종하여 새로 조성된 켄터키블루그래스의 성장에 질소와 인산의 영향을 측정하기 위해서 수행되었다. 질소의 시비량은 소량, 중량, 다량으로 각각 20, 30 and $40g\;m^{-2}\;yr^{-1}$로 되었으며 시비 횟수를 각각 2, 4, 6회로 구성되었다. 인산의 시비량은 0, 10 and $20g\;m^{-2}\;yr^{-1}$로 되었으며 인산은 질소시비와 함께 이루어 졌다. 소량의 질소시비는 실험기간 동안 최소수용 품질을 유지 하였으며 가장 적은 예지물을 생산하였다. 다량의 질소시비는 가장 좋은 잔디 품질을 나타내었다. 그러나 실험기간 동안 켄터키블루그래스의 성장에 대한 인산의 일관적인 영향은 발견되지 않았다. 두꺼운 대취층에 조성된 켄터키블루그래스의 성장에 인산의 영향이 없는 것과 동일하게 새로 조성된 켄터키블루그래스의 성장에도 인산의 영향은 일년 동안 발견되지 않았다.

• 한국작물학회지
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• 제67권2호
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• pp.121-130
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• 2022

본 연구는 봄 감자 재배 시 괴경 형성기와 괴경 비대기에 최적 수분함량을 구명하고 관비 재배 시 적합한 추비 기준을 마련하고자 수행 하였다. 1. 감자의 최적 수분함량을 구명하기 위한 괴경 형성기와 괴경 비대기에 관수처리 결과 관수처리 전후 지상부의 생육을 비교한 상대생장률과 대조구(무관수, 자연강우 의존) 대비 상대 생장률 모두에서 -10 kPa 및 -20 kPa 처리구에서 우수한 경향을 보였다. 2. 관수처리에 따른 지하부 생체중은 -10 kPa과 -20 kPa 처리구에서 다른 처리보다 뛰어났으며, 수확량 및 상서수량은 -10 kPa 처리구가 무관수구에 대비 약 47%가량 증수되었다. 3. 감자의 최적 관비량 설정을 위해 괴경 형성기와 괴경 비대기에 관비를 실시한 결과 관비 처리 전후 지상부의 생육을 비교한 상대생장률은 K 5 및 NPK 0.8배 관비구에서 생육이 가장 우수하였고, 대조구(무관비구, 기비만 시용) 대비 감자 지상부 상대 생장률은 K 5, K 7, NPK 1.2배 관비구에서 가장 우수하였다. 4. 관비 처리에 따른 감자 지하부 생체중 및 수량, 상서수량을 비롯한 다수의 조사항목에서 K 5 처리구가 가장 우수하였고, 수량도 K 5 처리구가 대조구 대비 약 27%가량 증수되었다.

• 한국토양비료학회지
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• 제46권6호
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• pp.652-658
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• 2013

A huge number of greenhouse soils in Korea have accumulated mineral elements which induce many nutritional and pathological problems. The present study was performed to the effects of the reduced fertilization on plant growth, and uptake and partitioning of minerals (N, P, K) and soluble carbohydrates using highly minerals-accumulated farmer's greenhouse soil. On the basis of the recommended application for tomato crop, the application rates of N, P and K were 110(50%)-5.2(5%)-41.5(35%)kg $ha^{-1}$, respectively, using Hoagland's nutrient solution. Tomato growth rates during the whole experiment were not significant between treatments, but it was found that a decrease in daily growth represented after 60 days of treatment (DAT). The reduced application led to a drastic decrease in the concentration of N, P and K in fruits, and, thus, this resulted in lower uptake after 40 DAT. The lower phloem export and utilization of soluble carbohydrates caused an accumulation of extra-carbohydrates in leaves, stems and fruits in the reduced application. The reduced fertilization induced the capture of N, P and K in leaves and of soluble carbohydrates in stems compared to the conventional application. In this study, we suggest that it is possible to delay the first fertigation time in minerals-accumulated soils without an adverse impact on crop growth, but it is necessary to regularly monitor mineral status in soil to ensure a balanced uptake, synthesis and partitioning of minerals and carbohydrates.

• 한국수정란이식학회지
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• 제31권3호
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• pp.153-159
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• 2016

(-)-Epicatechin gallate (ECG) is a polyphenol compound of green tea exhibiting biological activities, such as antioxidant and anticancer effects. To examine the effect of ECG on porcine oocytes during in vitro maturation (IVM), oocytes were treated with 0-, 5-, 15-, and $25{\mu}M$ ECG. After maturation, we investigated nuclear maturation, intracellular glutathione (GSH) and reactive oxygen species (ROS) levels and subsequent embryonic development after parthenogenetic activation (PA) and in vitro fertilization (IVF). After 42 hours of IVM, the $5{\mu}M$ group exhibited significantly increased (p< 0.05) nuclear maturation (89.8%) compared with the control group (86.1%). However, the $25{\mu}M$ group observed significantly decreased (p< 0.05) nuclear maturation (83.5%). In intracellular maturation assessment the 5-, 15-, and $25{\mu}M$ groups had significantly increased (p< 0.05) GSH levels and decreased ROS levels compared with the controls. The 5- and $15{\mu}M$ group showed significantly increased (p< 0.05) embryo formation rates and total cell number of blastocysts after PA (18% and 68.9, 15% and 85.1 vs. 12% and 59.5, respectively) compared with controls. Although the $25{\mu}M$ group observed significantly lower blastocyst formation rates after PA (27.6% vs. 23.2%) than control group, the $5{\mu}M$ group showed significantly increased blastocyst formation rates after PA (37.2% vs. 23.2%) compared to the control group. Furthermore, the $5{\mu}M$ group measured significantly increased blastocyst formation rates (20.7% vs. 8.6%) and total cell number after IVF ($88.3{\pm}1.5$ vs. $58.0{\pm}3.6$) compared to the control group. The treatment of $5{\mu}M$ ECG during IVM affectively improved the porcine embryonic developmental competence by regulating intracellular oxidative stress during IVM.

• 한국약용작물학회지
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• 제17권5호
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• pp.363-368
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• 2009

To fulfill the increasing demand for a high quality of flower, we investigated the effects of nitrogen application on plant growth, yield and bioactive compounds of Chrysanthemum indicum L.. C. indicum L. was cultivated in a pot scale, and nitrogen applied with the level of 0 (N0), 50 (N50), 100 (N100), 150 (N150), 200 (N200) and $300\;(N300)\;kg\;ha^{-1}$ to suggest optimum rate of nitrogen fertilization. Phosphate and potassium applied the same amount of $80-80\;kg\;ha^{-1}$ ($P_2O_5-K_2O$) in all treatments. Growth characteristics and yields of C. indicum L. were significantly affected by nitrogen application. Maximum yield achieved in 265 and $295\;kg\;ha^{-1}$ N treatment on the whole plant and the flower parts, respectively. The nitrogen content and uptake of whole plant significantly increased by the increase of nitrogen application. Five major components of essential oil, $\alpha$-pinene, 1,8-cineol, chrysanthenone, germacrene-D, and $\alpha$-curcumene in flowerheads of C. indicum L. occupied approximately 40% of peak area, germacrene-D decreased by the increase of nitrogen application among them. However, cumambrin A contents in the flower parts of C. indicum L. were affected negatively by the increase of nitrogen application, but total yields of cumambrin A in flower part significantly increased. Conclusively, nitrogen fertilization could increase the yield of flowerheads. The optimum application level of nitrogen fertilizer might be on the range of $265-295\;kg\;ha^{-1}$ in a mountainous soil.

• Journal of Ecology and Environment
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• 제32권3호
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• pp.149-158
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• 2009

American sycamore seedlings were grown in chambers with two different ozone concentrations ($O_3$-free air and air with additional $O_3$) for 45 days. Both the control and the $O_3$ chambers included non-fertilized and fertilized plants. After 18 days of $O_3$ fumigation, seedlings were placed in a clean chamber for 27 days. Seedlings under ozone fumigation showed a significant decrease in pigment contents and photosynthetic activity, and a significant increase in lipid peroxidation. Fertilization enhanced physiological damage such as the inhibition of photosynthetic activity and the increase of lipid peroxidation under ozone fumigation. During the recovery phase, the physiological damage level of seedlings increased with ozone fumigation. In addition, physiological damage was observed in the fertilized seedlings. Superoxide dismutase (SOD) and glutathione reductase (GR) activities of $O_3$-treated seedlings increased up to 33.8% and 16.3% in the fertilized plants. The increase of SOD activity was higher in the fertilized plants than in the non-fertilized plants. Negative effects of ozone treatment were observed in the biomass of the leaves and the total dry weight of the fertilized sycamore seedlings. The $O_3$-treated seedlings decreased in stem, root and total dry weight, and the loss of biomass was statistically significant in the fertilized plants. In conclusion, physiological disturbance under normal nutrient conditions has an effect on growth response. In contrast, in conditions of energy shortage, although stress represents a physiological inhibition, it does not seem to affect the growth response.

• 한국토양비료학회지
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• 제45권6호
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• pp.1187-1193
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• 2012

Water control is mainly one of the key factors that can affect nitrous oxide ($N_2O$) emissions from soils. This study was undertaken to determine the effect of intermittent drainage compared to continuous flooding (conventional water regime) on $N_2O$ emission to global warming potential (GWP) with NPK (standard cultivation practice), NPK+Straw, and PK fertilizations. Nitrous oxide emission rates were collected twice a week using a closed chamber method. With continuous flooding, nitrogen (N) application increased $N_2O$ emission by 106.6% ($0.64kg\;ha^{-1}$ in NPK) with respect to the PK treatment ($0.31kg\;ha^{-1}$), and straw addition to NPK enhanced 148.3% of seasonal $N_2O$ flux ($0.77kg\;ha^{-1}$ in NPK+Straw). Although seasonal $N_2O$ emission slightly increased by 16.1-42.9% with intermittent irrigation, its seasonal $CH_4$ emission drastically reduced at 43.5-52.8% resulting in a lower GWP at 48.9-58.5% with respect to that of continuously flooded treatments ($4.51Mg\;CO_2\;ha^{-1}$, PK; $7.60Mg\;CO_2\;ha^{-1}$, NPK; $14.55Mg\;CO_2\;ha^{-1}$, NPK+Straw). Rice yield, at similar fertilization with the continuously-flooded rice field, was not affected by intermittent irrigation. Conclusively, intermittent irrigation can be very effective and a rational soil management strategy to mitigate GWP with considering rice productivity in a temperate paddy rice field like Korea.