• 한국산림과학회지
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• 제58권1호
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• pp.17-22
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• 1982

은기(銀寄)밤나무 4년생(年生)을 1979년(年)부터 1980년(年)까지 2년간(年間) 시비시험(施肥試驗)을 실시(實施)하던 중(中) 1980년도(年度)에 일기불순(日氣不順)으로 일조량(日照量)이 부족(不足)하게 되었던 바 각처리별(各處理別) 시비시험중(施肥試驗中) 특(特)히 고토첨가시용구(苦土添加施用區)가 밤나무의 생장(生長)도 양호(良好)하고 밤 수확량(收穫량量)도 증대(增大)되었다. 1) 밤나무 생장(生長)에 있어서 동일시비수준(同一施肥水準)에서도 비료삼요소(肥料三要素)와 붕소(硼素) 및 고토(苦土) 시용구(施用區)가 삼요소시비구(三要素施肥區)보다 약(約)20% 정도생장(程度生長)이 양호(良好)하였다. 2) 밤수확량(收穫量)에 있어서도 시비시험일차년도(施肥試驗一次年度)인 1979년(年) 11月(월) 14일(日) 이상난동(異常暖凍)으로 인(因)하여 다소(多小)의 냉해피해(冷害被害)는 있었으나 일조량(日照量)은 정상적(正常的)이여서 6월(月)서 9월(月)까지 1일(日) 평균일조량(平均日照量)은 7시간(時間)으로 밤수확량(收穫量)은 시료삼요소(試料三要素)의 시비지수(施肥指數) 100에 대(對)하여 비료삼요소(肥料三要素) 및 붕소시비구(硼素施肥區)가 167이고 비료삼요소(肥料三要素) 붕소(硼素) 및 고토시비구(苦土施肥區)는 207dldT다. 3) 시비시험삼차년도(施肥試驗三次年度)인 1980년도(年度)에는 일기불순(日氣不順)으로 일조량(日照量)이 1일(日) 평균(平均) 3.8시간(時間)으로서 이와 같은 일조량(日照量) 부족(不足) 현상하(現狀下)에서는 밤수확량(收穫量)은 비료삼요소시비구(肥料三要素施肥區)의 시비지수(施肥指數) 100에 대(對)하여 비료삼요소(肥料三要素) 및 붕소함유복비시비구(硼素含有複肥施肥區)는 620이고 비료삼요소(肥料三要素), 붕소(硼素) 및 고토함유복비구(苦土含有複肥區)에서는 741로서, 비료삼요소(肥料三要素) 및 붕소시비구(硼素施肥區)보다도 비료삼요소(肥料三要素), 붕소(硼素) 및 고토(苦土) 시비구(施肥區)가 약(約) 21%가 더 증수(增收)되었으며 비료삼요소시비구(肥料三要素施肥區)보다는 7배이상(倍以上)의 밤수확(收穫)이 있었다. 4) 이상(以上)의 결과(結果)로 보아 고토(苦土)(MgO)가 토양중(土壤中) 0.82me/100g 미만(未滿)일때에는 MgO의 시비효과(施肥效果)가 크게 나타날 뿐더러 일조량부족시(日照量不足時)는 MgO의 시비(施肥)로 녹색식물(綠色植物)의 탄소동화작용(炭素同化作用)을 활발(活潑)히 하게 하며 엽록소(葉綠素)를 조성(造成)하는 작용(作用)을 돕는 동시(同時)에 탄수화물형성(炭水化物形成)에도 큰 역할(役活)을 하게 되는 것으로 입증(立證)되었다.

• 생물환경조절학회지
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• 제31권4호
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• pp.423-431
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• 2022

'탄소중립'에 대한 관심이 높아짐에 따라 CO₂를 농업적으로 이용하기 위한 시도가 증가하고 있다. 본 실험은 발전소에서 부산물로 배출되는 CO₂를 포집하여 액화·정제 후 시설 엽채류의 생육 및 생산성 증대를 위한 시비용 CO₂로의 활용 가능성을 평가하기 위해 수행되었다. 경상남도 하동지역의 부추, 취나물, 미나리 농장에 드라이아이스가 공급되었고 각 농장의 온실 중 하나의 온실은 대조군, 하나의 온실은 CO₂ 처리구로 사용되었다. CO₂의 시비는 자체 제작한 장치를 사용하여 드라이아이스에서 승화된 가스를 온실에 공급했다. 부추 온실은 대조군과 CO₂ 처리에서 온실 내 CO₂ 농도의 차이가 없었고 두 온실 모두 높은 CO₂ 농도를 보였다. 반면에 취나물과 미나리 온실에서는 CO₂ 시비 처리에서 높은 CO₂ 농도가 측정되었다. 취나물 및 미나리의 생육은 CO₂ 시비 처리구에서 유의성 있게 증가하였으며 수확량도 각각 36%와 25%로 증가하였다. 경제성 분석 결과, 취나물 농가에서는 소득률이 증가하였지만, 부추와 미나리 농가는 감소하는 것으로 나타났다. 따라서 화력발전소에서 부산물로 발생한 드라이아이스의 이용은 시설 엽채류의 생산성을 높일 수 있었다.

• 한국토양비료학회지
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• 제46권6호
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• pp.474-481
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• 2013

Understanding the microbial community structure of agricultural soils is important for better soil management in order to improve soil quality. Phospholipid fatty acid analysis has been popularly used in determining the microbial community structure in different ecosystems. The microbial community structure of paddy soil under long-term fertilizer treatments was investigated after 45 years using PLFA analysis. Treatments were control (no fertilization, Con), compost (COM), NPK, NPK+compost (NPKC), PK, NK, and NP. Soil chemical properties were mainly affected by the addition of compost and inorganic P fertilizer. Total nitrogen and organic matter contents were significantly higher in treatments with compost while available $P_2O_5$ and exchangeable calcium were significantly higher in treatments with added inorganic P fertilizer. It was found that microbial communities were responsive to the different fertilizer treatments. PLFA results showed that the soils were dominated by gram-negative bacteria, followed by the actinomycetes, then gram-positive bacteria, and fungi. Principal component analysis of the soil chemical properties and PLFA composition proved to be a more reliable tool because it was more responsive to the changes in soil chemical properties.

• 한국수정란이식학회지
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• 제9권3호
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• pp.261-268
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• 1994

Immatured bovine follicular oocytes added with serum, hormones, granulosa cells and bovine oviduct epithelium cells were fertilized in vitro after in vitro maturation. In vitro maturation and early development capacity were examined and IVF-derived embryos were transferred and to recipients and effects of sperm treatment on in vitro capacitation were investigated. The rate of in vitro maturation was improved when they were co-culutred with granulosa cells in the TCM199 medium added with 10% FCS and hormones. The percentage of acrosome reaction was not differed between sperm treatments and sperm of above 25% under-went AR during 30 min preincubation with caffeine and heparin. The cleavage rate of oocytes in vitro fertilized in TCM199 medium added with 10% FCS and hormones, GC or BOEG higher than that in medium with 10% FCS and GC. But the rate was not significantly different between GC and BOEG The cleavage of rate oocytes cultured in medium containing serum, hormones and BOEG was 80.2% and more embryos were developed to Blastocyst (17.3%). The selected embryos were transferred to 9 recipients by surgical or nonsurgical method but did not result in pregnancy.

• 아시안잔디학회지
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• 제13권3호
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• pp.159-170
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• 1999

Preference of chestnut brown chafer (CBC), Adoretus tenuimaculatus was examined from chestnut leaves which were treated with different light condition, temperature and nitrogen fertilization. More CBC was attracted to leaves which grown at $30^{\circ}C$ than grown at $20^{\circ}C$,$ 25^{\circ}C$ and in the field. When attracted number of CBC was compared among full sunlight-grown, cloth shaded-grown and dark-grown plants, 4.0 in full sunlight grown and 5.0 in dark-grown plants. Amount of nitrogen fertilizer did not influence CBC feeding. The attractiveness of undamaged leaves with non-feeding beetles, leaves with feeding damaged, and leaves with artificial damaged was compared in replicated laboratory trials by placing the treatments in the petri-dish and counted the number of beetles that landed on the plants after 6 hr treatment. The highest number of beetles was attracted to chestnut leaf with feeding damaged ($7.7\pm$0.6) than undamaged leaf with non-feeding beetles ($5.3\pm$0.6) and artificial damaged ($4.3\pm$0.6). Managnese content in the leaves of chestnut grown in shade cloth-grown condition was higher than that in the leaves of chestnut that had been exposed to full sunlight condition and dark condition, and feeding damaged leaf.

• 한국양식학회지
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• 제7권1호
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• pp.55-61
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• 1994

넙치의 수정란을 수정 3분 후 $2^{\circ}C$에서 45분간 저온 처리하여 $92.6\%$의 빈도로 3배체를 유도하였다 수정란의 부상율 및 수정율은 실험군 사이에 유의차가 없었다(P> 0.05). 그러나 부화율과 기형율은 2배체와 3배체가 유의차가 있게 나타났다P< 0.05). 3배체를 적혈구 세포크기 측정 및 염색체 분석으로 확인한 결과 세포의 표면적에서 3배체는 2배체에 비해 1.6배 증가되었고, 2배체의 염객체수는 48개의 acrocentric인 반면 3배체는 72개의 acrocentric 염색체로 나타났다.

• 대한한방부인과학회지
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• 제31권2호
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• pp.103-112
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• 2018

Objectives: The aim of this paper is to report that herb medicine effects on four infertility patients with ovarian cysts and the menstrual irregularity as a result of ovarian hyperstimulation syndrome. Methods: Four infertility patients with ovarian cysts had the same symptom of menstrual irregularity for preparing In Vitro Fertilization (IVF). They were treated by twice a day herb medication Changbudodam-tang. Then we observed the effects of treatments by regularity of menstruation, checking with ultrasonography of ovarian cyst size. Results: After herb treatments, the sizes of ovarian cysts decreased and one of them disappeared. Above all, menstrual regularity was recovered for preparing IVF. So they could have a positive progress of IVF. Conclusions: These cases show that herb medicine has its effective treatments for the implantational surroundings on infertility patients with ovarian cyst and menstrual irregularity because of ovarian hyperstimulation syndrome.

• Journal of Plant Biology
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• 제37권1호
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• pp.1-8
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• 1994

The effects of fetilization on the structure and function of an early successional Miscanthus sinensis grassland were investigated in an old-field ecosystem at Honjo city, Saitama Prefecture, Japan from 1981 to 1982. Compared to control plot, life form composition of treatment plot was characterized by a decrease of phanerophytes and an increase of hemicryptophytes. Fertilization had a beneficial effect on the growth in aboveground phytomass of M. sinensis, the dominant species. However, it did not cause a change in the total number of stems of M. sinensis. Annual increment of patch diameter of M. sinensis was estimated to be 6-8 cm on an average and there was no significant differecne in the growth rate of patch size between fertilized and nonfertilized plots. To estimate the aboveground phytomass of M. sinensis, several non-destructive parameters were examined. As a result, the patch size showed a high correlation with aboveground phytomass. Thus the patch size was suggested to be most applicable to its estimation. Diversity indices based on phytomass data of component species were increased slightly by fertilizatin, because relative dominance of some other species, especially of Artemisia princeps increased, while that of M. sinensis decreased. Fertilizer application resulted in a decrease in the total number of species.

• 한국수정란이식학회지
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• 제9권1호
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• pp.85-93
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• 1994

This study was carried out to investigate the effects of gonadotropins added during maturation of porcine oocytes on the in vitro maturation(IVM), in vitro fertilization(IVF) and developmental potential of embryos. The follicular oocytes were cultured in TCM-199 medium containing different combination of gonadotropins(5$\mu$g /ml FSR or 1OIU /ml PMSG and 1O$\mu$g /ml LH or 1OIU /ml hCG), 10% FCS and 10% PFF for 36~48h in a incubator with 5% $CO_2$ in Air at 39$^{\circ}C$ and then matured oocytes were again cultured to 120h after IVF for 6~7h with heparin(100$\mu$g /m')-treated sperm. When the oocytes were matured for 42brs in the medium containing FSH+LH, FSH+hCG, PMSG+LH or PMSG+hCG, the JVF rate of each treatment was 50.0%, 52.9%, 66.7% and 70.0%, respectively. The highest CEI (cumulus cell expansion index) was obtained from PMSG+hCG-added medium and the highest polyspermic penetration resulted from FSH+LH-added medium. The cleavage of IVF oocytes derived from hormone added IVM was significantly(P<0.05) promoted by PMSG+hCG and the cleavage rate after 36-h, 42-h and 48-h maturation aws 53.0%, 56.7% and 45.6%, respectively. The highest developmental potential resulted from the oocytes derived from PMSG+LH -added IVM.

• 인간식물환경학회지
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• 제24권5호
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• pp.499-507
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• 2021

Background and objective: This study was carried out to develop an effective technique for raising seedlings of Veronica pyrethrina Nakai, a native plant species in the Korean Peninsula, in plug trays. Methods: To investigate the optimum plug cell size and sowing media, we sowed seed in to plug trays with 34, 21, and 10 mL cells and filled with a commercial horticultural substrate and mixtures of peatmoss and perlite in 1:1, 3:1, and 4:1 ratios. Fertilization levels were set at 0, 500, 1000 and 2000 mg·L^-1. Results: Plug cell size did not significantly influence the seedling growth of V. pyrethrina. By substrate type, the growth rate was highest in the horticultural substrate, followed by 4:1, 3:1, and 1:1. Growth by fertilization level was higher in all fertilized treatment groups than in the control group, and there was no difference among 500, 1000, and 2000 mg·L^-1. Conclusion: The results of this study proved that it is most suitable for raising seedlings of V. pyrethrina to sow the seeds in a 10 mL cell plug tray filled with horticultural substrates, and apply fertilizers with less than 500 mg·L^-1 concentration.