• 한국식품영양학회지
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• 제35권5호
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• pp.313-323
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• 2022

To investigate antioxidant effects of tea tree root extracts using various extraction methods, cytotoxicity, DPPH and ABTS radical scavenging, SOD, nitrite scavenging activity and inhibitory activity of lipid peroxidation, reducing power, ferrous ion chelating activity were measured. Cytotoxicity for RAW 264.7 cells was not observed at concentrations treated with below 90 ㎍/mL in all extracts. The maximum DPPH radical, nitrite scavenging, SOD activity and inhibitory activity of lipid peroxidation were obtained at the ethylacetate and 70% ethanol extract. The maximum ABTS radical scavenging activity was obtained at the ethylacetate and hot water extract. However, in the case of reducing power and ferrous ion chelating activity, they were obtained at 70% ethanol and hexane extract, respectively. Nitrate scavenging activity showed the most excellent scavenging ability of 59.6% at 90 ㎍/mL of ethylacetate. The hexane extract had the highest ferrous ion chelating activity, showing 61.05% at 50 ㎍/mL, 66.07% at 70 ㎍/mL and 76.81% at 90 ㎍/mL, respectively. The results of this research show that the ethylacetate and 70% ethanol extracts of tea tree root can be used as a natural material for scavenging the radicals. However, future study is necessary to understand the mechanism of antioxidant activity by identification of substances.

• 한국자원식물학회지
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• 제23권2호
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• pp.123-130
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• 2010

본 연구는 식용 및 약용으로 사용되는 울릉미역취와 톱풀 어린잎의 항산화활성 및 생리활성에 미치는 가압열처리의 영향을 알아보기 위하여 시행하였다. 4월 초순에 충북청원군 노지에서 어린잎을 채취한 직후 가압열처리($121^{\circ}C$, 1.2기압, 15분) 또는 무처리하여 동결건조한 후 분쇄하여 80% 에탄올 용매로 환류냉각추출한 추출물의 총 폴리페놀 및 플라보노이드의 함량, DPPH와 ABTS radical 소거능 및 ferrou ion chelating 정도를 측정 하였다. 톱풀과 울릉미역취의 추출수율은 가압열처리한 울릉미역취에서 가장 높았고(39.55%), 가압열처리한 톱풀 어린잎에서 가장 낮았다(28.15%). 톱풀과 울릉미역취 어린잎은 가압열처리 후 DPPH radical 소거활성이 현저히 감소하여, 각각 $1.506\;mg{\cdot}mL^{-1}$, $0.669\;mg{\cdot}mL^{-1}$씩 $RC_{50}$값이 증가하였다. ABTS radical 소거활성 또한 각각 $0.704\;mg{\cdot}mL^{-1}$, $0.421\;mg{\cdot}mL^{-1}$ 씩 $RC_{50}$값이 증가하였다. 반면 ferrous ion chelate 효과는 가압열처리에 의하여 향상되었으며, 톱풀의 어린잎에서 큰 폭으로 상승하였다. 또한 가압열처리는 총 폴리페놀과 플라보노이드 함량을 감소시켰는데, 특히 톱풀 어린잎에서 감소폭이 컸다. 따라서 본 실험에서는 가압열처리는 울릉미 역취와 톱풀 어린잎의 Ferrous ion clelate 효과를 향상시킬 수 있지만, DPPH 및 ABTS radical 소거능과 총 폴리페놀 및 플라보노이드의 함량을 감소시키는 것으로 나타났다. 또한, 톱풀의 어린잎은 울릉미역취에 비하여 열안정성이 낮아 열처리에 의한 활성의 변화가 큰 것으로 나타났다.

• 한국환경과학회지
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• 제23권12호
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• pp.2129-2134
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• 2014

The antioxidant activities and total phenolics of four Rosaceae species Pourthiaea villosa (Thunb.) Decne, Sorbus commixta Hedlund, Sorbaria sorbifolia var. stellipila Maxim and Pyrus pyrifolia (Burm.f.) Nakai were determined. Phenolic content (polyphenol and flavonoid), radical scavenging activities [2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylnezthiazoline-6-sulfoic acid) (ABTS)] and ferrous ion chelating effect were evaluated. Total polyphenol and flavonoid contents were highest in Pourthiaea villosa (Thunb.) Decne and lowest in Pyrus pyrifolia (Burm.f.) Nakai. Phenolic contents of Pourthiaea villosa (Thunb.) Decne was $331.45{\pm}7.78$ and $90.4{\pm}3.5mg{\cdot}g^{-1}$. DPPH and ABTS radical scavenging activities were found to be lowest in Sorbus commixta Hedlund whereas Sorbaria sorbifolia var. stellipila Maxim and Pourthiaea villosa (Thunb.) Decne showed relatively good DPPH and ABTS radical scavenging activities. Ferrous ion chelating effect was highest in Pyrus pyrifolia (Burm.f.) Nakai ($1.05{\pm}0.04mg{\cdot}ml^{-1}$) and lowest in Sorbus commixta Hundlund ($4.22{\pm}0.71mg{\cdot}ml^{-1}$).

• 한국응용과학기술학회지
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• 제32권2호
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• pp.312-319
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• 2015

In this study, n-butanol and ethyl acetate extracts were prepared from raw yam (Dioscorea batatas DECNE.). Their antioxidative potencies were investigated employing various in vitro methods, such as ferrous ion chelating, ${\beta}$-carotene bleaching assay, lipid peroxidation inhibition and nitric oxide (NO) radical scavenging and nitrite scavenging activity. The n-butanol fraction was assayed to possess stronger antioxidant activity by ${\beta}$-carotene bleaching assay, lipid peroxidation inhibition and NO radical scavenging activity. However, ethyl acetate extract was more effective in chelating ferrous ion and scavenging nitrite. Based on the results obtained, yam is a potential active ingredient that could be applied in antioxidation as well as bio-health functional food to take a good part in prevention of human diseases and aging.

• 한국식품영양과학회지
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• 제39권4호
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• pp.631-636
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• 2010

천연 항산화제를 개발하기 위하여 80% 에탄올을 용매로 환류냉각 추출한 마가렛, 국화 및 낙동구절초의 꽃과 잎줄기(shoot)의 페놀성 물질 함량, DPPH radical과 ABTS radical 소거능, ferrous ion chelating 효과 및 linoleic acid에 대한 지질과산화 억제활성을 측정하였다. 총 폴리페놀 함량, 총플라보노이드 함량, DPPH radical 소거능, ABTS radical 소거능은 국화 잎줄기 추출물에서 가장 높았으며, 특히 국화 잎줄기 추출물의 ABTS radical 소거능은 천연 항산화제인 ascorbic acid와 합성 항산화제인 BHT와 유사하였다. 그러나 $Fe^{2+}$ chelating 효과는 국화 잎줄기 추출물에서 가장 낮았으며, 국화 꽃 추출물에서 가장 우수하였다. Linoleic acid에 대한 지질과산화 억제활성은 마가렛과 국화 잎줄기 추출물에서 가장 우수하였으나, BHT보다 억제활성이 낮았다. 연구의 결과, 국화 잎줄기 추출물은 페놀성 물질 함량 및 radical 소거활성이 우수하여 천연 항산화제로 개발 가치가 매우 높은 것으로 생각되었다. 그러나 금속이온 chelating 및 지질 과산화 억제활성은 다소 낮으므로, 다발적으로 발생하는 산화스트레스를 방지하기 위한 항산화제를 개발하고자 할 때는 국화 잎줄기 추출물과 다른 항산화제를 같이 사용하는 것이 좋을 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제31권6호
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• pp.905-913
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• 2018

Objective: To evaluate the effects of L-lysine (Lys)/L-arginine (Arg) on lipid and protein oxidation of emulsion sausage during storage and its possible mechanism. Methods: Four samples were prepared based on the presence or absence of additional sodium isoascorbate, Lys, or Arg: sample A (control), sample B (0.05 g of sodium isoascorbate), sample C (0.4 g of Lys), and sample D (0.4 g of Arg). Peroxide value (POV), thiobarbituric reactive substances (TBARS), protein carbonyls and thiols were measured. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical-scavenging, ferrous ion-chelating ability were also measured. Results: Compared with the control, the sample treated with sodium isoascorbate, Lys or Arg had significantly lower POV during the initial 20 days, TBARS during the initial 15 days. Protein carbonyls were significantly lower compared Sample B, C, and D with A during the later storage (10 to 25 days); basically, protein thiols became lower during storage when the samples were treated with sodium isoascorbate, Lys, or Arg. Both Lys and Arg had weak reducing power but strong ferrous ion-chelating activity and DPPH radical- and hydroxyl radical-scavenging activity. Conclusion: Both Lys and Arg effectively inhibited the oxidation of lipids and proteins in emulsion sausage by scavenging free radicals and chelating ferrous ions. The results obtained may be favorable for the prevention of lipid and protein oxidation during processing and storage of meat products.

• Preventive Nutrition and Food Science
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• 제16권4호
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• pp.321-327
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• 2011

Water, methanol and ethanol extracts of ginseng leaves were assayed for total phenolics and flavonoids, ascorbic acid, cupric and ferrous ion chelating activities, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP) assay and ABTS radical cation decolourization (TEAC) assay for their antioxidant properties. The ethanol extract of ginseng leaves contained significantly (p<0.05) higher amounts of total phenolics and flavonoids (600.57 and 1701 mg/100 g) than methanol (374.43 and 1512.64 mg/100 g) and water extracts (248.30 and 680.05 mg/100 g). Among solvent extracts of ginseng leaves, the ethanol extract showed the most powerful antioxidant activities. However, the ferrous ion chelating activity of ginseng leaf extracts were lower than the cupric ion chelating ability. These differences in concentrations of key antioxidants among various solvent extracts seemed to be responsible for their differences in antioxidant activities. These results suggest that ethanol extract of ginseng leaves has the most effective antioxidant capacity compared to the methanol and water extracts tested in the present study. Thus, it can be applied for the effective extraction of functional material from ginseng leaves for the usage of pharmaceutical and/or food industries.

• 한국환경과학회지
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• 제28권4호
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• pp.393-401
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• 2019

The present study were conducted to determine physiological activities and antioxidant effects [2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity, reducing power, Ferric Reducing Antioxidant Power (FRAP) and Fe2+ (ferrous ion) chelating capacity] of 70% methanol, chloroform:methanol, 2:1 volume ratio (CM) and ethyl acetate extract of turmeric (Curcuma longa L.). Bioactive compound of tannin $0.125{\pm}0.007mg$ Catechin Equivalent (CE)/g dry weight. Turmeric extracts yield were 70% methanol 16.54%, CM 5.64% and ethyl acetate 4.14%, respectively. Antioxidant activity of the samples exhibited a dose-dependent increase. Results showed that extraction solvent had significant effects on total flavonoid content and antioxidant effects of ethyl acetate. But ferrous ion-chelating capacity of 70% methanol extract was higher than CM and ethyl acetate extract. From the results of this study, turmeric can be utilized as a valuable and potential nutraceutical for the functional food industry.

• 한국자원식물학회지
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• 제23권1호
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• pp.47-53
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• 2010

Dendranthema속의 산국, 감국 및 울릉국화의 꽃과 잎줄기(Shoot)를 80% 에탄올을 용매로 환류냉각추출하여 페놀성물질 함량, DPPH radical과 ABTS radical 소거능, ferrous ion chelating 효과 및 linoleic acid에 대한 지질 과산화 억제활성을 측정하였다. 연구의 결과, 총 폴리페놀 및 플라보노이드 물질 함량은 울릉국화에서 가장 높았으며 특히, 울릉국화 꽃에서 함량이 높았다. DPPH radical과 ABTS radical 소거능 또한 울릉국화 꽃에서 가장 우수하였으며, 울릉국화의 잎줄기에서도 소거능이 높게 나타났다. 그러나 ferrous ion chelating 효과는 산국 꽃에서 가장 높았으며, 울릉국화 꽃에서 가장 낮았다. 지질과산화 억제활성은 울릉국화 잎줄기에서 가장 높았으며, 반응 32일 이후에도 41.01%의 높은 억제활성을 보여 합성 항산화제인 BHT보다 지질산패 억제효과가 우수하였다. 연구의 결과, 울릉국화 꽃 및 잎줄기는 항산화물질함량이 높고 항산화활성이 우수하므로 천연 항산화소재로 적합하였다. 또한 동일한 식물도 부위에 따라 항산화효과가 다른 것을 확인 하였으며, 항산화효과의 종류에 따라 우수한 활성을 보이는 식물종이 각기 다르므로 사용 목적에 따라 식물종을 선택적으로 사용해야 할 것으로 생각되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권4호
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• pp.567-573
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• 2005

This study aims to evaluate the antioxidative activities of cow-milk kefir and goat-milk kefir. Antioxidative mechanisms, including radical-scavenging effects, ferrous-ion chelating ability, reducing power and antioxidant activity, were investigated herein. Kefirs demonstrated significantly greater scavenging effects upon 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide radicals, an inhibition effect upon linoleic-acid peroxidation, and more substantial reducing power, but reduced glutathione peroxidase (GSH-Px) activity than was the case for milks. There was no significant difference between milks and kefirs as regards ferrous-ion chelating ability and superoxide dismutase (SOD) activity. These findings have demonstrated that kefirs possess antioxidant activity, thereby suggesting that kefirs are potential candidates for the role of useful natural antioxidant supplements for the human diet.