• Journal of Acupuncture Research
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• 제22권5호
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• pp.21-28
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• 2005

Objectives : The following study was undertaken in order to seek the acupuncture operation method of ${\ll}$Sang Han Lun(傷寒論)${\gg}$ Liu-Jing-Bian-Zheng(六經辯證). Methods : Based on the documents quoted in ${\ll}$Sang Hang Za Bing Lun Xu Wen(傷寒雜病論 序文)${\gg}$ of 'Zhang, Zhong-Jing(張仲景)', the relativity of the theory of Jin-Mai(硬脈) and Liu-Jing-Bian-Zheng of convalescence, and from the Liu-Jing-Bing(六經病), the origin and implication that caused so-Yin-Bing(少陰病) to form was studied on the basis of acupuncture medicine publications and the commentary writing of ${\ll}$Sang Han Lun${\gg}$. Results : 1. ${\ll}$Sang Han Lun${\gg}$ Liu-Jing-Bian-Zheng has succeeded and was developed based on Liu-Jing-Fen-Zheng(六經分證) Of ${\ll}$Su Wen Re Lun(素問 熱論)${\gg}$. In addition, the summary of Liu-Jing-Bing became the general principle of Fen-Jing(分經) and Ding-Zheng(定證) that may be applicable to Fenghan(風寒), Wenre(溫熱), Lili(疫?) and Eating(雜病). 2. Most commentators of ${\ll}$Sang Han Lun${\gg}$ in the Song, Ming and Ching Dynasties of ${\ll}$Sang Han Lun${\gg}$ interpreted the so-yin-Bing in physiological and pathological aspects of Rong-Wei(榮衛) as the disease of the Heart and Kidney meridian. 3. From the Liu-Jing-Bing of ${\ll}$Sang Han Lun${\gg}$, the region of acupuncture treatment of So-yin-Bing is treated with the needle from the point of view of Bing-Zheng-Lun-Zhi(辨證論治) with the basis of the important region of acupuncture of the heart of meridian Kindey of meridian.

• Journal of Acupuncture Research
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• 제21권6호
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• pp.159-166
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• 2004

The following study was undertaken in order to seek the acupuncture operation method of $\ll$Sang Han Lun(傷寒論)$\gg$ Liu-Jing-Bian-Zheng(六經辯證). Based on the documents quoted in $\ll$Sang Hang Za Bing Lun Xu Wen(傷寒雜病論 序文)$\gg$ of "Zhang, Zhong-Jing(張仲景)", the relativity of the theory of Jing-Mai(經脈) and Liu-Jing-Bian-Zheng of convalescence, and from the Liu-Jing-Bing(六經病), the origin and implication that caused Tai- Yin-Bing(太陰病) to form was studied on the basis of acupuncture medicine publications and the commentary writing of $\ll$Sang Han Lun$\gg$. As a result of the foregoing study, the author has written the following acupuncture operation on the basis of a summarized Tai-Yin-Bing for $\ll$Sang Han Lun$\gg$ Liu-Jing-Bian-Zheng. Results : $\ll$Sang Han Lun$\gg$ Liu-Jing-Bian-Zheng has succeeded and was developed based on Liu-Jing-Fen-Zheng(六經分證) of $\ll$Su Wen Re Lun(素問 熱論)$\gg$. In addition, the summary of Liu-Jing-Bing became the general principle of Fen-Jing(分經) and Ding-Zheng(定證) that may be applicable to Fenghan(風寒), Wenre(溫熱), Lili(疫려) and Zabing(雜病). 2. Most commentators of $\ll$Sang Han Lun$\gg$ in the Song, Ming and Ching Dynasties of $\ll$Sang Han Lun$\gg$ interpreted the Tai-Yang-Bing in physiological and pathological aspects of Rong-Wei(榮衛) as the disease of the spleen meridian. 3. From the Liu-Jing-Bing of $\ll$Sang Han Lun$\gg$, the region of acupuncture treatment of Tai-Yang-Bing is treated with the needle from the point of view of Bing-Zheng-Lun-Zhi(辨證論治) with the basis of the important region of acupuncture of the spleen meridian.

• Journal of Acupuncture Research
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• 제24권2호
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• pp.203-210
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• 2007

Objectives: The following study was undertaken in order to seek the acupuncture operation method of ${\ll}$Sang Han Lun(傷寒論)${\gg}$ Liu-jing-Bian-Zheng(六經辨證) Methods : Based on the documents quoted in ${\ll}$Sang Han Lun ${\cdot}$ Xu Wen(傷寒雜病論 ${\cdot}$ 序文)${\gg}$ of "Zhang, Zhong-jing(張仲景)", the relativity of the theory of jing-Mai(經脈) and Liu-Jing-Bian-Zheng of convalescence, and from the Liu-jing-Bing(六經病), the origin and implication that caused Yang-Ming-Bing(陽明病) to form was studied on the basis of acupuncture medicine publications and the commentary writing of ${\ll}$Sang Han Lun${\gg}$. Results: 1. ${\ll}$Sang Han Lun${\gg}$ Liu-jing-Bian-Zheng has succeeded and was developed based on Liu-jing-Fen-Zheng(六經分證) of ${\ll}$Su Wen ${\cdot}$ Re Lun(素問 ${\cdot}$ 熱論)${\gg}$. In addition, the summary of Liu-jing-Bing became the general principle of Fen-jing(分經) and Ding-Zheng(定證) that may be applicable to Fenghan(風寒), Wenre(溫熱), Lili and Zabing(雜病). 2. Most commentators of ${\ll}$Sang Han Lun${\gg}$ in the Song, Ming and Ching Dynasties of ${\ll}$Sang Han Lun${\gg}$ interpreted the Yang-Min-Bing in physiological and pathological aspects of Rong-Wei(榮衛) as the disease of the Stomach meridian and Large Intestine meridian. 3. From the Liu-Jing-Bing of ${\ll}$Sang Han Lun${\gg}$, the region of acupuncture treatment of Yang-Min-Bing is treated with the needle from the point of view of Bing-Zheng-Lun-Zhi(辨證論治) with the basis of the important region of acupuncture of the Stomach of meridian ${\cdot}$ Large Intestine of meridian.

• 생명과학회지
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• 제15권6호
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• pp.1005-1012
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• 2005

식물 내생균 Bacillus sp. CY22는 식물병원균 Rhizoctonia solani, Fusarium oxysporum 및 Phythium ultimum에 대해 강한 항균력을 나타내었다. 일반적으로 많은 Bacillus속 균주들은 iturin, fengycin, mycosubtulin과 같은 항균 물질을 분비한다. 본 연구에서는 식물내생균 Bacillus sp. CY22의 배양액으로부터 항균물질을 분리, 정제하였으며 MALDI-TOF mass로 분자량을 확인하였다. MALDI-TOF mass spectrum분석 결과 분리된 항균물질은 Bacillus 속 균주가 생성하는 항균물질로서 잘 알려져 있는 iturin의 분자량과 거의 일치하였으며, m/z 1043.4, 1057.4, 1071.4에서 molecular ion peak를 나타내었다. 이들은 각각 m/z 14차이를 가진 iturin의 isoform으로 추정되며 이 것은 iturin을 구성하고 있는 지방산의 탄소수 차이로 생각되며 m/z 1065.4, 1079.4 peak는 sodium adduct로서 추정된다. 또한 항균물질 iturin을 생성하는데 관여하는 transacylase 유전자를 크로닝하여 ita22 유전자로 명명하고, 그 특성으로 ita22 유전자는 400 개의 아미노산을 인지하는 1,200 bp의 open reading frame (ORF)을 가지며, 아미노산의 상동성을 조사한 결과 Bacillus subtilis 168의 FenF (BAB69697)와 가장 유사하였다.

• 동양고전연구
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• 제54호
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• pp.155-182
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• 2014

이 논문은 "중용"의 "천명지위성(天命之謂性)"과 "시중(時中)", 그리고 "천지위만물육(天地位萬物育)"의 이슈가 진화생물학의 유전자, 자연선택, 종 다양성 문제와 대화 가능한지 그 가능성을 검토하고 있다. 이를 위해 먼저 성리학과 진화생물학의 유사점과 차이점을 살펴보고, "중용"과 진화생물학의 접점이 무엇인지 모색하고 있다. 이 논문은 세 가지 점에서 "중용"과 진화생물학 사이의 대화 가능성을 검토한다. 첫번째는 인간 본성에 대해 천명지위성과 유전자의 관점에서 대화 가능한지 살펴본다. 두 번째는 존재 이유의 타당성을 시중과 자연선택의 관점에서 살펴본다. 세 번째는 생물간 상호 유연 관계를 천지위만물육과 종 다양성이라는 관점에서 살펴본다. 첫 번째 모색의 소주제들은 <리와 기 vs 유전자와 운반체>의 구조적 연관성, <성선 vs 이기적 유전자의 이타성>의 판단기준, <인 의 예 지 vs 후성규칙>에서 '후천적 선험' 도출 가능성이다. 두 번째 모색의 소주제들은 <천리의 뒤집힘[번(?)] vs 돌연변이>, <변/화 vs 진화>의 시간관, <시중 vs 자연선택/적응>의 메타meta적 접근이다. 세 번째 모색의 소주제들은 <리일분수理一分殊 vs 부분과 전체>의 프랙탈 구조, < 일기一氣 vs 생물간 상호 유연>의 상동관계, <천지위 만물육 vs 생태계>와 치중화致中和의 과제이다. 이러한 모색과 제안들에 대한 후속연구는 "중용"을 둘러싼 성리학 연구의 지평 확대를 가져 올 수 있다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2797-2802
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• 2007

It has been confirmed that implementation of the no-slip boundary conditions for the lattice-Boltzmann method play an important role in the overall accuracy of the numerical solutions as well as the stability of the solution procedure. We in this paper propose a new algorithm, i.e. the method of the dynamic boundary condition for no-slip boundary condition. The distribution functions on the wall along each of the links across the physical boundary are assumed to be composed of equilibrium and nonequilibrium parts which inherit the idea of Guo's extrapolation method. In the proposed algorithm, we apply a dynamic equation to reflect the computational slip velocity error occurred on the actual wall boundary to the correction; the calculated slip velocity error dynamically corrects the fictitious velocity on the wall nodes which are subsequently employed to the computation of equilibrium distribution functions on the wall nodes. Along with the dynamic selfcorrecting process, the calculation efficiently approaches the steady state. Numerical results show that the dynamic boundary method is featured with high accuracy and simplicity.

• Bulletin of the Korean Chemical Society
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• 제25권4호
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• pp.545-548
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• 2004

A sensitive, selective and rapid method for the determination of nickel based on the rapid reaction of nickel(II) with QADMAA and the solid phase extraction of the Ni(II)-QADMAA chelate with $C_{18}$ membrane disks has been developed. In the presence of pH 6.0 buffer solution and sodium dodecyl sulfonate (SDS) medium, QADMAA reacts with nickel to form a violet complex of a molar ratio of 1 : 2 (nickel to QADMAA). This chelate was enriched by solid phase extraction with $C_{18}$ membrane disks. An enrichment factor of 50 was obtained by elution of the chelates form the disks with the minimal amount of isopentyl alcohol. The molar absorptivity of the chelate was $1.32{\times}10^5L\;mol^{-1}cm^{- 1}$ at 590 nm in the measured solution. Beer's law was obeyed in the range of 0.01-0.6 ${\mu}$g/mL. This method was applied to the determination of nickel in water and biological samples with good results.

• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5883-5886
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• 2012

Osteosarcoma is the most common primary bone malignancy in children and adolescents, and its clinical outcome is poor. We evaluated the response of GSTP1, ERCC1 and ERCC2 to chemotherapy among osteosarcoma patients, and the role of these genes on the prognosis of osteosarcoma. 187 patients with osteosarcoma were administered with methotrexate, cisplatin/adriamycin, actinomycin D, cyclophosphamide, or vincristine treatment. GSTP1, ERCC1 and ERCC2 polymorphism was genotyped by PCR-RFLP assay. The results showed the average survival time of 187 patients were 38.4 months. 97 patients showed response to neoadjuvant chemotherapy. The GSTP1 Val and ERCC2 A/A genotypes had significantly higher rates of response to chemotherapy, with adjusted OR (95% CI) of 2.19 (1.15-6.21) and 2.88 (1.14-13.25). Individuals with ERCC2 A/A genotype were likely to have a lower risk of death from oseosarcoma, and the adjusted HR was 0.32 (0.13-0.95). Our study indicated test of GSTP1 and ERCC2 Lys751Gln polymorphisms might be a candidate pharmacogenomic factors to be explored in the future to identify the osteosarcoma patients who might benefit from chemotherapy.

• Asian Pacific Journal of Cancer Prevention
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• 제13권9호
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• pp.4793-4799
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• 2012

Hypoxia is commonly featured during glioma growth and plays an important role in the processes underlying tumor progression to increasing malignancy. Here we compared the gene expression profiles of rat C6 malignant glioma cells under normoxic and hypoxic conditions by cDNA microarray analysis. Compared to normoxic culture conditions, 180 genes were up-regulated and 67 genes were down-regulated under hypoxia mimicked by $CoCl_2$ treatment. These differentially expressed genes were involved in mutiple biological functions including development and differentiation, immune and stress response, metabolic process, and cellular physiological response. It was found that hypoxia significantly regulated genes involved in regulation of glycolysis and cell differentiation, as well as intracellular signalling pathways related to Notch and focal adhesion, which are closely associated with tumor malignant growth. These results should facilitate investigation of the role of hypoxia in the glioma development and exploration of therapeutic targets for inhibition of glioma growth.

• Bulletin of the Korean Chemical Society
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• 제25권2호
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• pp.263-266
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• 2004

A sensitive, selective and rapid method has been developed for the determination ${\mu}$g/L level of vanadium ion based on the rapid reaction of vanadium(V) with 2-(2-quinolylazo)-5-diethylaminophenol (QADEAP) and the solid phase extraction of the colored chelate with $C_{18}$ cartridge. The QADEAP reacts with V(V) in the presence of citric acid-sodium hydroxide buffer solution (pH = 3.5) and cetyl trimethylammonium bromide (CTMAB) medium to form a violet chelate of a molar ratio 1 : 2 (V(V) to QADEAP). This chelate was enriched by solid phase extraction with $C_{18}$cartridge and the enrichment factor of 50 was obtained by elution of the chelates from the cartridge with ethanol. The molar absorptivity of the chelate is $1.28 {\times}10^5L\;mol^{-1}cm^{-1}$ at 590 nm in the measured solution. Beer's law is obeyed in the range of 0.01-0.6 ${\mu}$g/mL. The detection limit is 0.04 ${\mu}$g/L in the original samples. This method was applied to the determination of vanadium(V) in water and biological samples with good results.