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http://dx.doi.org/10.5352/JLS.2005.15.6.1005

Identification and Molecular Characterization of Three Isoforms of Iturin Produced by Endophytic Bacillus sp. CY22  

Cho, Soo-Jeong (Laboratory of Microbial Functions, Bioprocess Engineering Division, Korea Research Institute of Bioscience and Biotechnology)
Yun-Han-Dae (Division of Applied Life Science, Gyeongsang National University, Research Institute of Agriculture & Life Sciences, Gyeongsang National University)
Publication Information
Journal of Life Science / v.15, no.6, 2005 , pp. 1005-1012 More about this Journal
Abstract
Endophytic Bacillus sp. CY22 was previously isolated from the interior of balloon flower root and showed strong antifungal activity against phytopathogenic fungi such as Rhizoctonia solnni, Fusarium oxysporum, and Phythium ultimum. Many Bacillus strains produce antifungal compound such as iturin, fengycin, and mycosubtilin. We isolated and identified antifungal compound from cell supernatant of the endophytic strain. By the MALDI-TOF mass result, the antifungal compound was similar to the known antifungal lipopeptide iturin. It was found that the purified iturin had three isoforms with protonated masses of m/z 1,043.39, 1,057.42, and 1,071.42 and different structures in combination with $Na^{+}$ ion using MALDI-TOF MS. The ita22 gene, which transacylase gene is associated with production of antifungal iturin, had an open reading frame (ORF) of 1,200 bp encoding 400 amino acids. Results of deduced amino acids sequence homology search, Ita22 was homologous with FenF (BAB69697) of Bacillus subtilis 168.
Keywords
Endophytes; Bacillus sp.; Iturin isoforms; MALDI-TOF mass;
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