• Title/Summary/Keyword: feces

Search Result 1,464, Processing Time 0.023 seconds

Change in composition of gut microbiota by exposure of natural medicines including Glycyrrhizae Radix in mice

  • Jeon, Yong-Deok;Song, Young-Jae;Jin, Jong-Sik
    • Proceedings of the Plant Resources Society of Korea Conference
    • /
    • 2018.10a
    • /
    • pp.126-126
    • /
    • 2018
  • Many of researches have revealed that human intestinal microbiota is related to health. Several diseases like obesity, diabetes, and hypertension are affected by the microbiota directly and indirectly. So, interventions with food and drug have been tried to change a composition of the microbiota to better condition. However, few natural medicines have elucidated to date. To understand an influence on microbiota by plant materials including Glycyrrhizae Radix, the extract of medicines were administered to mice and the feces were collected before and after the administration. The feces were analyzed by terminal restriction fragment length polymorphism (T-RFLP). The changes in composition of mice gut microbiota were detected and analyzed. The data could be utilized to further study about biological activities of the plant medicines.

  • PDF

Adhesive Properties of Lactobacillus brevis FSB-1 In Vivo

  • Kim, Seong-Yeong;Shin, Kwang-Soon;Lee, Ho
    • Food Science of Animal Resources
    • /
    • v.30 no.4
    • /
    • pp.560-567
    • /
    • 2010
  • This study was conducted to evaluate the in vivo gastrointestinal survival and adhesive properties of orally administered Lactobacillus brevis FSB-1. ELISA conducted using polyclonal antibodies specific for L. brevis FSB-1 was able to detect the organism in feces; therefore, we used ELISA to determine the concentration of lactic acid bacteria in feces collected from Wister rats that had been administered $10^{10}$ cells/rat/d orally for 20 d. The mean recovery of L. brevis FSB-1 was approximately $10^{7.22}$ cells/g of wet feces during the oral administration period, and $10^{7.50}$ and $10^{7.46}$ at 8 and 10 d after the end of oral administration, respectively. These results indicate that L. brevis FSB-1 was able to survive in the gastrointestinal tract of rats, and that it had a high adhesive property in rat colons.

Design and implementation of Mobile U-health Service Platform - Feces and Urine Sensing U-Care Scheme -

  • Min, Byoung-Won;Oh, Yong-Sun
    • International Journal of Contents
    • /
    • v.5 no.4
    • /
    • pp.62-68
    • /
    • 2009
  • This paper presents a novel method to design and implement mobile u-health system by defining the essential elements of mobile healthcare services. We choose common service elements for the proposed u-healthcare scheme and design the service platform. Especially we focus on automatic feces or urine sensing u-care scheme to prove the effectiveness of our platform. We construct the system with sensing part with a manikin and a diaper wireless communication part with feces or urine sensing data, and coordinator system based on the u-health platform defined in this paper. Experimental results show that our scheme is useful in the area of u-care service for the handicapped, the elderly, and patients who can hardly move by themselves. In addition the designed scheme offers a realized u-care scheme with the purpose of advanced developing tools for application or service developers.

Complete genome sequence of Streptococcus hyointestinalis B19, a strain producing bacteriocin, isolated from chicken feces

  • Lee, Ju-Eun;Heo, Sunhak;Kim, Geun-Bae
    • Journal of Animal Science and Technology
    • /
    • v.62 no.3
    • /
    • pp.420-422
    • /
    • 2020
  • Streptococcus hyointestinalis B19 was isolated from chicken feces collected from local farm in Anseong, Korea. S. hyointestinalis B19 was shown to produce bacteriocin-like compounds exhibiting inhibitory activities against several pathogens including strains of Clostridium perfringens and Listeria monocytogenes. The whole genome of S. hyointestinalis B19 strain was sequenced using PacBio RS II platform. The genome comprised four contigs with a size of 2,217,061 bp. The DNA G + C content was found to be 42.95 mol%. Annotation results revealed 2,266 coding sequences (CDSs), 18 rRNAs, and 61 tRNA genes. Based on genome analysis, we found that the strain B19 possessed various genes associated with bacteriocin synthesis, modification, and transport.

Probiotic Characteristics of Lactobacillus plantarum FH185 Isolated from Human Feces

  • Park, Sun-Young;Lim, Sang-Dong
    • Food Science of Animal Resources
    • /
    • v.35 no.5
    • /
    • pp.615-621
    • /
    • 2015
  • Lactobacillus plantarum FH185 was isolated from the feces of healthy adults. In our previous study, L. plantarum FH185 was demonstrated that it has anti-obesity effect in the in vitro and in vivo test. In order to determine its potential for use as a probiotic, we investigated the physiological characteristics of L. plantarum FH185. The optimum growth temperature of L. plantarum FH185 was 40℃. L. plantarum FH185 showed higher sensitivity to novobiocin in a comparison of fifteen different antibiotics and showed higher resistance to polymyxin B and vancomycin. It also showed higher β-galactosidase and N-acetyl-β-glucosaminidase activities. Moreover, it was comparatively tolerant to bile juice and acid, and inhibited the growths of Salmonella Typhimurium and Staphylococcus aureus with rates of 44.76% and 53.88%, respectively. It also showed high adhesion activity to HT-29 cells compared to L. rhamnosus GG.

Dehydrating and bacterial elimination effects of fecal dehydrating system for reducing bovine mastitis derived from environmental contamination (환경유래 젖소유방염 저감을 위한 우분뇨 탈수 시스템의 탈수 및 유방염 원인체 제균 효과 규명)

  • Kim, Dong Hyeok;Lim, Jung Ju;Lee, Jin Ju;Kim, Dae Geun;Chang, Hong Hee;Lee, Seung Joo;Lee, Yun Beom;Chang, Dong Il;Lee, Hu Jang;Min, Won-Gi;Kim, Sang Hun;Oh, Kwon Young;Kim, Suk
    • Korean Journal of Veterinary Research
    • /
    • v.49 no.3
    • /
    • pp.257-263
    • /
    • 2009
  • Bovine mastitis is an important disease causing serious economic loss in dairy production and food poison in public health. Escherichia coli and Staphylococcus aureus are the major causative agents of bovine mastitis. These bacteria were found in milk and environmental condition such as feces, water, soil and so on. Bovine mastitis causative micro-organisms can survive in 1-2 weeks in feces and bed complexes. Low level of percentage of water content (PWC) of feces and bed complexes can reduce the spreading of bovine mastitis incidence from environmental contamination. In this study, we developed the fecal dehydrating system and determined the elimination rates of bovine mastitis causative agent from feces and bed complexes. To develop the fecal dehydrating system, the screw pressurized dehydrating system was used and the maximum rate of dehydrating was reached to 52% PWC using 90% PWC (wet base) of fecal and bed complexes. The elimination rates of the dehydrating system for E. coli and S. aureus were reached at 41.19 $\pm$ 7.84% to 62.55 $\pm$ 8.71% in various percentages of PWC of feces and bed complexes (80, 85 and 90%). These results suggested that the application of fecal dehydrating system would reduce the exposure of dairy cattle to bovine mastitis causing agents contaminated feces and bed complexes, and can be used for environmental bovine mastitis control avoiding misuse or abuse of chemical disinfectants and antibiotics in dairy farm.

Potential Probiotic Properties of Lactobacillus johnsonii IDCC 9203 Isolated from Infant Feces (유아 분변에서 분리한 Lactobacillus johnsonii IDCC 9203의 잠재적 프로바이오틱 특성)

  • Lee, Seung-Hun;Yang, Eun-Hee;Kwon, Hyuk-Sang;Kang, Jae-Hoon;Kang, Byung-Hwa
    • Microbiology and Biotechnology Letters
    • /
    • v.36 no.2
    • /
    • pp.121-127
    • /
    • 2008
  • A strain IDCC 9203 isolated from infant feces was identified as Lactobacillus johnsonii on the basis of 16S rDNA sequence analysis. L. johnsonii IDCC 9203 was highly resistant to acid (MRS broth at pH 2.3) and bile (MRS broth with 0.3% oxgall). The antibacterial activities of L. johnsonii IDCC 9203 was examined against Salmonella typhimurium KCTC 2054. The growth of S. typhimurium KCTC 2054 was inhibited by the cell-free culture supernatant (at pH 4.0) of L. johnsonii IDCC 9203 as well as by the respective control (MRS broth at pH 4.0). Antimicrobial effect against S. typhimurium KCTC 2054 of L. johnsonii IDCC 9203 was probably due to the lactic acid. By an in vitro cell adhesion model, L. johnsonii IDCC 9203 preincubated or coincubated with Caco-2 cells reduced the adhesion of S. typhimurium KCTC 2054 to Caco-2 cells by 74% or 47.1%, respectively. Also in an in vivo model, L. johnsonii IDCC 9203 was colonized in mice intestines which were disrupted by ampicillin treatment. Its proliferation in the mice intestines reduced abnormal salmonella growth from $10^9CFU/g$ feces to $10^5CFU/g$ feces as an indigenous level. The results obtained in this study suggest that L. johnsonii IDCC 9203 may be a potential probiotic strain.

Differences in microbiome and virome between cattle and horses in the same farm

  • Park, Jongbin;Kim, Eun Bae
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.33 no.6
    • /
    • pp.1042-1055
    • /
    • 2020
  • Objective: The ecosystem of an animal farm is composed of various elements, such as animals, farmers, plants, feed, soil, and microorganisms. A domesticated animal's health is largely connected with the reservoir of bacteria and viruses in animal farms. Although a few studies have focused on exploring the gut microbiome of animals, communities of microbiota and viruses in feedlots have not been thoroughly investigated. Methods: Here, we collected feces and dust samples (4 groups: cattle feces, C_F; horse feces, H_F; cattle dust, C_D; and horse dust, H_D) from cattle and horse farms sharing the same housing and investigated their microbiome/virome communities by Illumina sequencing. Results: Dust groups (C_D and H_D) showed higher microbial diversity than feces groups (C_F and H_F) regardless of animal species. From the microbial community analysis, all the samples from the four groups have major phyla such as Proteobacteria (min 37.1% to max 42.8%), Firmicutes (19.1% to 24.9%), Bacteroidetes (10.6% to 22.1%), and Actinobacteria (6.1% to 20.5%). The abundance of Streptococcus, which commonly recognized as equine pathogens, was significantly higher in the horse group (H_D and H_F). Over 99% among the classified virome reads were classified as Caudovirales, a group of tailed bacteriophages, in all four groups. Foot-and-mouth disease virus and equine adenovirus, which cause deadly diseases in cattle and horse, respectively, were not detected. Conclusion: Our results will provide baseline information to understand different gut and environmental microbial ecology between two livestock species.

Studies on Physico-chemical Properties an d Pathogenicity of Porcine Enterovirus Isolated from Feces of Pigherds (돼지 분변 유래 PORCINE ENTEROVIRUS의 물리화학적 특성 및 병원성에 관한 연구)

  • 박정우;이종인;신용호;조우영;최윤식
    • Korean Journal of Veterinary Service
    • /
    • v.14 no.2
    • /
    • pp.110-120
    • /
    • 1991
  • 28 porcine enteroviruses were isolated from 86 pig-feces of 9 swine farms located in south region, Chung-buk, from March to September 1990. Physico-chemical properties and pathogenicity of isolates were investigated. Results obtained throughout experiments are summarized as follows. According to the age, weanlings(40-90 days), sucklings(10-30 days) and adult pigs(6 months over) showed the isolation rate of 67%. 8% and 4%, respectively. By physico-chemical tests, YD-90/22, YD-90/43 and YD-90/64 strains were found to be ether, chloroform and PH stable. Nucleic acid test suggests the virus to have a DNA genome. Most of the Isolates were not evident of hemagglutinin using erythrocytes from various mammalian & avian. 22 strains among the isolates were shown CPE type I and the remainders were CPE type II. 3 strains among isolates of CPE type I strains were neutralized with high titers to serotype 2 antiserum. In the study on virus growth curve in PK-l5 cells, YD-90/22, YD-90/43 and YD-90/64 strains showed the maximum infectivity titers($10^{6.0}-l0^{6.5} TCID({50}ml$) at 4days post inoculation(PI). When 30 day-old commercial piglets were inoculated only intraoral route with the YD-90/22 strain at $10^{6.0} TCID_{50}ml,$ piglets not showed the symptoms. But piglets inoculated by intramuscle route, intraoral and intramuscle route after pretreat with dexamethasone(2.5mg /kg) for 5 days were shown the symptoms of anorexia, diarrhea, pyrexia and ataxia at 4th-6th days PI. The viral reisolation in the virus-inoculated piglets was examined from feces. The viruses were recovered intermittently from 2nd to 16th day PI and at 4th-6th day PI, all piglets excreted viruses.

  • PDF

Effects of Xylooligosaccharide Intake on Fecal Bifidobacteria, Lactic acid and Lipid Metabolism in Korean Young Women (자일로올리고당의 섭취가 변 내 비피더스 균수, Lactic Acid 농도와 지질대사에 미치는 영향)

  • Na, Mi-Hee;Kim, Woo-Kyoung
    • Journal of Nutrition and Health
    • /
    • v.40 no.2
    • /
    • pp.154-161
    • /
    • 2007
  • This study investigated the effects of xylooligosaccharide on feces bifidobacteria proliferation, lactic acid concentration and lipid metabolism in healthy woman. Fourteen volunteers were randomly assigned to 2 groups : 1.4 g/day xylooligosaccharide intake group, 2.8 g/day xylooligosaccharide intake group. The duration of the study was 28 days. The amount of feces and excretion time were not affected by xylooligosaccharide intake. The color of feces changed to yellow brown, and hardness of stool and effort to evacuation were reduced by xylooligosaccharide intake. Xylooligosaccharide intake reduced the fecal pH significantly after 14 days in 2.8 g/day intake group (p < 0.05). The number of fecal bifidobacteria were significantly increased after 28 days in 1.4 g/day intake group (p < 0.05), and in 2.8 g/day intake group, the number of fecal bifidobacteria significantly increased after 14 days (p < 0.05). Water contents of feces were not affected by xyloolgosacchride intake. The fecal triglyceride and cholesterol concentrations were increased in 2.8 g/day intake group (p < 0.05), and in 1.4 g/day intake group, fecal cholesterol concentration only was increased (p < 0.05). The fecal lactic acid concentration was significantly increased in 2.8 g/day intake group (p < 0.05). Serum trigly-ceride, cholesterol and glucose concentration were significantly decreased in 2.8 g/day intake group (p < 0.05). In conclusion, xylooligosaccharide dietary supplementation may be beneficial to gastrointestinal health and lipid metabolism, and 2.8 g/day intake was more effective than 1.4 g/day intake.