Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Potential Probiotic Properties of Lactobacillus johnsonii IDCC 9203 Isolated from Infant Feces

유아 분변에서 분리한 Lactobacillus johnsonii IDCC 9203의 잠재적 프로바이오틱 특성

  • Lee, Seung-Hun (ILDONG Research Laboratories, ILDONG Pharmaceutical Co., Ltd.) ;
  • Yang, Eun-Hee (ILDONG Research Laboratories, ILDONG Pharmaceutical Co., Ltd.) ;
  • Kwon, Hyuk-Sang (ILDONG Research Laboratories, ILDONG Pharmaceutical Co., Ltd.) ;
  • Kang, Jae-Hoon (ILDONG Research Laboratories, ILDONG Pharmaceutical Co., Ltd.) ;
  • Kang, Byung-Hwa (ILDONG Research Laboratories, ILDONG Pharmaceutical Co., Ltd.)
  • Published : 2008.06.28
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Abstract

A strain IDCC 9203 isolated from infant feces was identified as Lactobacillus johnsonii on the basis of 16S rDNA sequence analysis. L. johnsonii IDCC 9203 was highly resistant to acid (MRS broth at pH 2.3) and bile (MRS broth with 0.3% oxgall). The antibacterial activities of L. johnsonii IDCC 9203 was examined against Salmonella typhimurium KCTC 2054. The growth of S. typhimurium KCTC 2054 was inhibited by the cell-free culture supernatant (at pH 4.0) of L. johnsonii IDCC 9203 as well as by the respective control (MRS broth at pH 4.0). Antimicrobial effect against S. typhimurium KCTC 2054 of L. johnsonii IDCC 9203 was probably due to the lactic acid. By an in vitro cell adhesion model, L. johnsonii IDCC 9203 preincubated or coincubated with Caco-2 cells reduced the adhesion of S. typhimurium KCTC 2054 to Caco-2 cells by 74% or 47.1%, respectively. Also in an in vivo model, L. johnsonii IDCC 9203 was colonized in mice intestines which were disrupted by ampicillin treatment. Its proliferation in the mice intestines reduced abnormal salmonella growth from $10^9CFU/g$ feces to $10^5CFU/g$ feces as an indigenous level. The results obtained in this study suggest that L. johnsonii IDCC 9203 may be a potential probiotic strain.

IDCC 9203은 모유 영양아의 분변으로부터 분리하였으며 분자생물학적 동정 결과 Lactobacillus johnsonii로 밝혀졌다. L. johnsonii IDCC 9203은 pH 2.3으로 조정된 MRS 액체배지에서 2시간 반응시 81%의 생존율을 보였으며, 0.3% bile이 함유된 MRS 액체배지에서는 5시간 반응시 57%의 생존율을 보여 산과 담즙에 대한 내성이 있는 것으로 나타났다. L. johnsonii IDCC 9203은 in vitro에서 Caco-2 cells에 대한 부착능이 우수하여 S. typhimurium KCTC 2054의 부착을 경쟁적으로 50%이상 감소시켰다 또한 in vivo에서 L. johnsonii IDCC 9203은 항생제로 교란된 mice의 장내에 정착하여 증식하였으며 이상 증식된 salmonella ($10^9CFU/g$ feces)를 초기 수준 ($(10^5CFU/g$ feces)로 감소시켰다. 이상의 결과에서 L. johnsonii IDCC 9203은 사람 및 동물에 유용한 프로바이오틱 특성을 가지고 있어 정장용 유산균으로 개발될 수 있을 것으로 기대된다.

Keywords

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