• 한국해양생명과학회지
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• 제2권2호
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• pp.56-61
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• 2017

본 연구에서는 신안 장산도 남부해역 육상오염원의 위생상태와 영향 반경을 분석하였다. 배수유역에 대한 해안선 조사를 실시해 오염원 종류를 구분하고, 해역으로 방출되는 오염원 유량과 위생실태를 분석하였으며 오염원의 영향 반경을 산정하였다. 육상오염원은 생활하수 21개소, 농업용수 1개소, 육상양식장 11개소로 총 33개소(St. 65~97)이었고 이 중 농업용수 1개소, 육상양식장 8개소의 오염원이 해역으로 배출되었다. 방출오염원의 유량은 72,857~281,250 l/min이었고, 분변계대장균은 St. 72(농업용수)에서 490 MPN/100 ml, St. 74(육상양식장)에서 49 MPN/100 ml으로 비교적 높았다. 이들 영향 반경은 각각 4,389 m와 1.900 m로 나타났고, 해역의 안전한 위생을 확보하기 위해서는 이들 오염원들에 대한 관리 및 해수 위생조사가 필요할 것으로 판단된다.

• Fisheries and Aquatic Sciences
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• 제19권4호
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• pp.17.1-17.9
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• 2016

The seasonal variation of fecal coliforms (FCs) and physicochemical factors was determined in seawaters of the Hansan-Geojeman area, including a designated area for oyster, and in inland pollution sources of its drainage basin. The mean daily loads of FCs in inland pollution sources ranged from $1.2{\times}10^9$ to $3.1{\times}10^{11}$ most probable number (MPN)/day; however, the pollutants could not be reached at the designated area. FC concentrations of seawaters were closely related to season, rainfall, and inland contaminants, however, within the regulation limit of various countries for shellfish. The highest concentrations for chemical oxygen demand (COD) and $chlorophyll-{\alpha}$ in seawaters were shown in the surface layer during August with high rainfall, whereas the lowest for dissolved oxygen (DO) in the bottom layer of the same month. Therefore, it indicates that the concentrations of FC, COD, DO, and $chlorophyll-{\alpha}$ of seawaters were closely related to season and rainfall.

• 한국해양학회지
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• 제5권2호
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• pp.65-72
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• 1970

지상의 각 하천수계는 적지않은 세균류에 의한 오염을 받고 있어, 이것은 우리의 환경위생 또는 보건위생면에서 큰 관심사가 될 뿐 아니라 이것들은 결국 해역으로 유입되어 이곳에 서식하는 각종 연안수족을 오염하여 우리의 식품위생면에서도 많은 주의를 환기시키고 있다. 환경수의 세균오염에는 여러 가지가 있으나 여기서는 분변성 오염세균에만 한정한다. 환경수에 혼입되는 분변성 오염세균에는 대장균군, 대장균 및 장구균 등이 있으며, 이것은 우리나라의 하천수에도 적지않은 량이 출현하고 있으며 (최,김 1970) 특히 도시폐수의 주입이 많은 곳에서는 이것들에 의한 오염이 심대할 것이 예상된다. 그러나 이러한 세균들은 담수 및 해수에서 유래되는 것이 아니므로, 담수역에 있어서 또는 해역으로 유입된 후의 동태가 매우 흥미롭다.

• 한국환경보건학회지
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• 제36권2호
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• pp.141-147
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• 2010

Indicator bacteria of fecal pollution were enumerated and compared by various detection methods for influent and final effluent of a sewage treatment plant. Total coliforms were enumerated by four methods including most probable numbers, chromogenic enzyme substrate test, membrane filtration, and plate counts and were about $10^4$ for influent and $10^2{\sim}10^3\;CFU/ml$ for final effluent. Fecal coliforms ranged between $10^3$ and $10^4$ for influent and $10^2\;CFU/ml$ for effluent by chromogenic enzyme substrate test and membrane filtration. Fecal streptococci counts were 1-log less than fecal coliforms counts, $10^2{\sim}10^3$ for influent and $10^1\;CFU/ml$ for effluent. Total coliforms numbers by plate count both in influent and in effluent showed 1-log higher than by the other three methods. Statistical analysis revealed that numbers of total coliforms by plate count in final effluent had the highest average of correlation (r=0.778, p<0.01) compared with those by the other three methods. In addition, total coliforms numbers by plate count showed most significant correlation (r=0.835, p<0.01) with those by chromogenic test which is well-known as its highest recovery efficiency. These results suggest that the plate count would be the optimum detection method for total coliforms in wastewater treatment plants which are the only microbiological standard of final effluent from wastewater treatment plants in the Republic of Korea, considering economic aspects and difficulties in laboratories.

• 보존과학연구
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• 통권26호
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• pp.103-126
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• 2005

Coprostanol is a metabolic product of cholesterol, formed by microbial action in the mammalian gut. This chemical compound is the major sterol in human and has been routinely studied as a biomarker of sewage pollution in marine and lacustrinesediments. This has led to the search for coprostanol as a biomarker in archaeologicalsoils, in order to detect the presence of fecal material. In this study, five samples of archaeological soils excavated at Wanggung ri, Iksancity, were used to assess the possibility of using coprostanol as indicators of ancient human activity in archaeological areas. The sampled soils were analyzed MXRD,EDXRF for their physical and chemical properties. And coprostanol was analysed byGC/MSD, using SIM method to detect and quantify specific compound. The results showed the soils were composed of quartz and feldspars, inorganicelement such as $SiO_2$, $Al_2O_3$, $Fe_2O_3$ etc. Moreover, the result from the analysis wasindicated that the specific compound is coprostanol. The coprostanol was determined at $0.16~1.01\mug$/g in the range of concentrations. This finding indicate that clear promise exists for the exploitation of coprostanol as biomarker of ancient human activity inarchaeological survey. Therefore such studies can serve to increase the confidence we place on biomarker-based methodologies for assessing fecal pollution. The application of this methodology has proved a simple and effective way of searching for that pattern in successively more aged deposits either known or suspected to contain fecal material. And the more scientific analysis of the soils will be showed to utility of the area ancient dietary life style, ancient environment.

• Journal of Animal Science and Technology
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• 제66권1호
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• pp.125-134
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• 2024

In this research, the growth efficiency, nutritional utilization, fecal microbial levels, and fecal score of weaned pigs were evaluated using therapeutic zinc oxide (ZnO) and zinc aspartic acid chelate (Zn-Asp). In a 42-day feeding trial, 60 weaned pigs ([Yorkshire × Landrace] × Duroc) were arbitrarily allotted (age: 21 days; 7.01 ± 0.65 kg preliminary body weight) to 3 different treatment groups with 5 repetitions (2 male and 2 female piglets) in each pen. The trial had 2 different phases, including 1-21 days as phase 1, and 22-42 days as phase 2. The nutritional treatments were: basal diet as control (CON), basal diet incorporated with 3,000 ppm ZnO as TRT1, and basal diet incorporated with 750 ppm Zn-Asp as TRT2. In comparison to the CON group, the pigs in the TRT1 and TRT2 groups had greater (p < 0.05) body weight on day 42; an average daily gain, and an average daily feed intake on days 22-42. Furthermore, during days 1-42, the average daily gain in the treatment groups trended higher (p < 0.05) than in the CON group. Additionally, the fecal score decreased (p < 0.05) at week 6, the lactic acid bacteria count tended to increase (p < 0.05), and coliform bacteria presented a trend in reduction (p < 0.05) in the TRT1 and TRT2 groups compared to the CON group. However, there was no difference in nutrient utilization (p > 0.05) among the dietary treatments. Briefly, the therapeutic ZnO and Zn-Asp nutritional approaches could decrease fecal score and coliform bacteria, increase lactic acid bacteria, and improve growth efficiency; moreover, Zn-Asp (750 ppm) can perform a comparable role to therapeutic ZnO (3,000 ppm). So we can use Zn-Asp (750 ppm) instead of therapeutic ZnO (3,000 ppm) for the better performance of weaning pigs and the reduction of environmental pollution, as therapeutic ZnO is responsible for environmental pollution.

• 한국환경보건학회지
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• 제39권6호
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• pp.513-521
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• 2013

Objectives: This study was performed in order to detect indicator bacteria in drinking spring water (DSW) samples in Seoul Metropolitan City, and to identify their genus through 16S rRNA sequencing and then assessing the genetic relation of their strains. Methods: For indicator bacteria detection and identification of total coliforms, we analyzed DSW between the spring and summer seasons. In particular, DSW samples were chosen from sites repeatedly found unsatisfactory in recent years. Results: Heterotrophic plate counts of DSW in the spring and summer season were investigated in the range of 0-550 and 0-800 CFU/mL, respectively. Total coliforms of these were 0-1,900 and 0-2,100 CFU/100mL, fecal coliforms were 0-600 and 0-550 CFU/100mL, and Escherichia coli were 0-7 and 0-326 MPN/100mL. The detection ratio of fecal pollution indicators and that of fecal coliforms increased to 58.6% in the summer from 12.5% in the spring and Escherichia coli increased to 51.4% from 4.7%. As a result of genetic analysis on the isolated bacteria, the genus of total coliforms was classified in the order of Enterobacter spp. 12.7%, Serratia spp. 7.3%, E. hermanii 6.4%, Rahnella spp. 5.5%, Hafnia spp. 4.5%, Escherichia coli 3.6%, Klebsiella spp. 3.6% in the spring season. In the summer season, it was classified in order of Klebsiella spp. 16.6%, Enterobacter spp. 13.0%, Escherichia coli 11.0%, Serratia spp. 8.6%, Raoultella spp. 7.0%, Kluyvera spp. 5.6% and Citrobacter spp. 3.0%. Conclusions: The increase of fecal pollution in summer indicates that special attention to drinking DSW is required.

• Journal of Preventive Medicine and Public Health
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• 제7권1호
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• pp.175-176
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• 1974

The purpose of this study was to survey bacteria which was known as a fecal bacteria living in the human and animal gastro and intestinal tract. The colony density of the fecal coliform and fecal streptococci was 280 and 130 in 100ml sample amounts reapectively. Staphylococcus aureus and Pseudomonas aeruginosa which is usually exist in swimming pool and was known as indicator of the pool water contamination was detected.

• 한국수산과학회지
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• 제45권1호
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• pp.11-16
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• 2012

In coastal areas that are affected by continuous, seasonal or occasional pollution sources, bivalves accumulate biological contaminants such as pathogenic bacteria. We investigated the effect of natural seawater relaying and electrolyzed seawater depuration on the bacteriological quality of artificially contaminated oysters Crassostrea gigas and short-necked clams Ruditapes philippinarum to suggest an alternative method of shellfish sanitation control.When artificially contaminated oysters and short-necked clams (fecal coliform level 1,700 MPN/100 g) were relayed into a sea area of safe bacteriological water quality, the fecal coliform level dropped to below 110 MPN/100 g after 1 day. The bacteriological quality of oysters and short-necked clams that are contaminated at a fecal coliform level of 1,700 MPN/100 g could be improved, and become appropriate for raw consumption by a single day relay under proper environmental conditions. When artificially contaminated oysters (fecal coliform level 330 MPN/100 g) were depurated with electrolyzed seawater, 94% of fecal coliform was eliminated after 12 h and fecal coliform was undetectable after 24 h. After 24 h depuration with electrolyzed seawater, the fecal coliform level of short-necked clams with initial fecal coliform of 2,400 MPN/100 g was below 20 MPN/100 g. However, the fecal coliform level of short-necked clams with initial fecal coliform of 17,000 MPN/100 g was relatively high, at 790 MPN/100 g, even after 24 h of depuration with electrolyzed seawater, because of the repeated cycle of excretion and accumulation of fecal coliform in shellfish tissue under the closed depuration environment. Such natural seawater relaying and electrolyzed seawater depuration can be restrictively applied to improve or secure the bacteriological quality of oysters and short-necked clams in accordance with safety levels for bivalves for raw consumption.

• 한국수산과학회지
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• 제56권5호
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• pp.615-625
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• 2023

This study evaluated the bacteriological safety of seawater and abalone produced in Uisin-myeon, Jindo-gun from September 2020 to December 2022 and calculated the effect of inland pollution sources on seawater and abalone. We surveyed 33 stations for seawater, 4 stations for abalone, and 7 stations for discharge water of major inland pollutants. We analyzed the coliform group, fecal coliform and Escherichia coli (E. coli) as bacteria indicators. In 924 seawater samples, the geometric mean of the coliform group and fecal coliform were <1.8-5.6 MPN/100 mL and <1.8-2.7 MPN/100 mL, respectively. The estimated 90^th percentile range for the coliform group and fecal coliform were <1.8-42 MPN/100 mL and <1.8-8.4 MPN/100 mL, respectively. The fecal coliform and E. coli levels for 107 abalone were <18-460 MPN/100 g and <18-78 MPN/100 g respectively. Moreover, the analyzed standard plate count was <30-7,700 CFU/g. The fecal coliform level and diffusion area in 7 inland pollutants were <1.8-3,300,000 MPN/100 mL and 2-2,500 m, respectively. The bacteriological safety of seawater and abalone in Uisin-myeon, Jindo-gun, was demonstrated to be a designated area according to Korean standards, a conditionally approved area per US standards, and Class A according to EU standards.