• Journal of Animal Science and Technology
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• v.54 no.5
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• pp.341-347
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• 2012

The purpose of this study was to evaluate the effects of dietary Flammulina velutipes mycelium (FVM) on broiler chick performance, pathogenic bacterial (E. coli, Salmonella) counts in caecal contents and amount of $NH_3$ in excreta. Ninety-six broiler chicks (HanHyup No. 3, Korea) were divided into four groups: 1) Control (basal diet), 2) T1 (supplemented with 1% FVM), 3) T2 (3% FVM), and T3 (5% FVM), and rose for 7 weeks. In results, there were no significant differences among treatments in weight gain, feed intake, feed efficiency and carcass yield. However, giblets were significantly increased in FVM treatments compared with control group (p<0.05). The number of Salmonella in caecum was significantly decreased in FVM treatments compared with control group (p<0.05). The number of E. coli was decreased in T2 but increased in T1 and T3 compared with control group. The emission of fecal $NH_3$ gas was significantly decreased in accordance with increasing the feeding level of FVM (p<0.05). In conclusion, our data indicated that the supplementation of high level of FVM could inhibit the Salmonella in caecum and reduce the emission of fecal $NH_3$ gas. Therefore, FVM at 5% level could be added in the diet of broiler chicks.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.9
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• pp.1309-1315
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• 2012

A total of 140 weaning pigs ((Landrace${\times}$Yorkshire)${\times}$Duroc, BW = $6.47{\pm}0.86$ kg) were used in a 5-wk growth trail to determine the effects of phytoncide supplementation on growth performance, nutrient apparent total tract digestibility (ATTD), blood profiles, diarrhea scores and fecal microflora shedding. Pigs were assigned randomly by BW into 5 treatments, dietary treatments were: i) NC, basal diet; ii) PC, NC+0.05% tylosin; iii) EO, NC+0.1% essential oil; iv) PP, NC+0.2% PP (phytoncide with 2% citric acid), and v) PA, NC+0.2% PA (phytoncide). Each treatment had 7 replicate pens with 4 pigs per pen. All pigs were housed in pens with a self-feeder and nipple drinker to allow ad libitum access to feed and water throughout the experimental period. During 0 to 2 wks, supplementation with essential oil and PA decreased (p<0.05) G/F compared with the other treatments. During 2 to 5 wks, supplementation with PA led to a higher (p<0.05) G/F than the other treatments. At 2 wk, ATTD of dry matter (DM) and gross energy (GE) in EO treatment were decreased (p<0.05) compared with NC treatment. Dietary PC treatment improved (p<0.05) ATTD of DM and E compared with the CON group, and PA and PP treatments showed a higher (p<0.05) ATTD of E than that in NC treatment. Pigs fed phytoncide (PA and PP) had a greater (p<0.05) ATTD of DM than those of NC and EO treatments at 5 wk. Moreover, supplementation with phytoncide elevated (p<0.05) the concentration of immunoglobulin (IgG) in blood at 2 wk. The inclusion of EO, PP and PA treatments showed a greater (p<0.05) amount of fecal Lactobacillus compared with CON group. However, no difference (p>0.05) was observed in diarrhea scores among treatments. In conclusion, phytoncide can elevate feed efficiency, nutrient digestibility, and improve the fecal Lactobacillus counts in weaning pigs. Our results indicated that the phytoncide could be used as a good antibiotics alternative in weaning pigs.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.2
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• pp.84-90
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• 2001

This study was performed to know the effect of depolymerized alginate obtained by hydrolysis of alginate through a heating process at $121^{\circ}C$ on intestinal environment, Rats were fed with diets containing $1\%$, $5\%$, and $10\%$ of each depolymerized alginate (HAG-10, HAG-50, HAG-100 and alginate) for 35 days, The changes of weight, moisture content, pH and volatile basic nitrogen (VBN) of fecal, and a short chain fatty acids (SCFA) were checked in the rats. The fecal weight and moisture content were the highest in rats fed with alginate diets (p<0.01), followed by HAG-100, HAG-50 and HAG-10 in order. The $5\%$ of HAG-50 diets induced a significant increase in contents of protein and lipid of feces, resulting in the decrease of apparent digestibility of protein and lipid (p<0.01). The pH and VBN content in feces of the rats decreased in $5\%$ and $10\%$ of HAG-50 diets, but $10\%$ of HAG-100 diets; $5\%$ and $10\%$ of alginate diets brought about an increase of fecal pH and VBN (p<0.01), The amount of n-butyric acid in feces was increased while propionic and acetic acid contents decreased significantly (p<0.01) in diets containing $5\%$ and $10\%$ HAG-50. However, the feces of rat fed diet containing $5\%$ and $10\%$ alginate showed a tendency to being opposite in results than that of HAG-50.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.3
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• pp.469-473
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• 2003

This study was conducted to determine the effect of a mixture of two water-soluble dietary fibers, psyllium husk and glucomannan, on serum lipids, fecal fat excretion and body fat. Twenty one female Sprague-Dawley rats were randomly assigned into three groups (control, pair-fed control and test group) and fed a high saturated fat (20% lard) diet (control diet) or a test diet (psyllium husk and glucomannan added to the control diet at the level of 0.9% and 0.68%, respectively) for 8 weeks. Pair-fed control rats were allowed to eat the amount of diet consumed by test group the preceding day. The body weight gain and food efficiency ratio of test group were significantly lower compared with these of control group. The mean values of body weight gain and food efficiency ratio were lower in test group than in pair-fed control group, but the difference was not significant. There were no differences in food intake. The dry weight and water content of feces and fecal fat excretion were markedly greater in test group than in control groups. Serum total cholesterol and triacylglycerol levels were significantly lower in test group than in two control groups.1'he rats of two control groups had higher body fat contents than that of test group. These results indicate that the combination of the two water-soluble dietary fibers, psyllium husk and glucomannan, can be used as a potent lipid-lowering agent in individuals consuming high saturated fat diet.

• Korean Journal of Pharmacognosy
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• v.50 no.1
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• pp.37-45
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• 2019

Portulaca oleracea L. (PL) has been used in traditional medicine herb for treatment of various diseases, such as diarrhea, dysentery, and skin inflammation. Previous studies have shown that the PL regulates the inflammation by inhibition of pro-inflammatory cytokines. Although PL might have improvement effects of intestinal function and bioactive effects, there are not enough studies to demonstrate. This study investigated the effects of KDC16-2 on the improvement of intestinal function and anti-inflammatory effects in vivo and in vitro. The improvement effect of intestinal function was measured fecal amount, water content and intestinal transit rate in KDC16-2 treated ICR mice. As results, compared with the control group, the KDC16-2 group showed a significant increase in wet fecal weight, dry fecal weight and fecal water content. The intestinal transit rate of KDC16-2 group was significantly increased. Based on the results, KDC16-2 is considered to have effects on improving intestinal function. The effect of anti-inflammatory demonstrated by using dextran sulfate sodium (DSS)-induced colitis mice. The mice were administered 3% DSS along with KDC16-2 (100, 300 mg/kg) for 14 days. DSS-induced colitis mice were significantly ameliorated in KDC16-2 treated group, including body weight loss, colon length shortening, tight junction protein of colon and histological colon injury. The levels of inflammatory mediators (IgG2a, IgA, C-reactive protein and Myeloperoxidase) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-${\alpha}$, Interleukin (IL)-6) which are involved in inflammatory responses were increased in the DSS-treated group as compared to those in the control group, and the levels were significantly decreased in the KDC16-2 groups. In addition, we investigated the impact of KDC16-2 on lipopolysaccharide (LPS)-induced inflammatory responses in J774A.1 cells. KDC16-2 inhibited production of prostaglandin E2 (PGE2) and reactive oxygen species (ROS). These results suggested that the KDC16-2 could effectively alleviate the dysfunction of intestinal and inflammatory mediators. Thus, these KDC16-2 can be potentially used as health functional food of intestinal.

• Preventive Nutrition and Food Science
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• v.6 no.1
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• pp.66-72
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• 2001

Ginseng may have antioxidant and pharmacologic effects similar to those of vitamin E. The interactive effect of ginseng and vitamin E was studied with respect to cholesterol metabolism and the antioxidant status. A ginseng supplement (0.1%, wt/wt) with comparable levels of vitamin E was provided with a high-cholesterol (1%, wt/wt) diet to rats for 5 weeks. The amount of vitamin E included in the ginseng-free and ginseng diets was either a low (low-E) or a normal (normal-E) level. The ginseng supplements significantly (p<0.05) altered the concentrations of plasma triglycerides in both the low-vitamin E group and normal-vitamin E group compared to the each ginseng-free group. The hepatic triglyceride and cholesterol content were not significantly (p>0.05) different between groups regardless of the vitamin E level in the diet. The hepatic HMG-CoA reductase activity was significantly (p<0.05) lowered by the ginseng supplement in both the low-vitamin E and the normal-vitamin E groups compared to the ginseng-free group. The HMG-CoA reductase activity was also significantly (p<0.05) lowered with in increase of the dietary vitamin E in the ginseng-free group. The excretion of fecal neutral sterol was significantly (p<0.05) lower in the normal-E ginseng group than th low-E ginseng-free group. Neither dietary ginseng nor vitamin E significantly changed the hepatic antioxidant enzymes activity. This data indicates that ginseng supplements lower the concentration of plasma triglyceride and hepatic HMG-CoA reductase activity regardless of eh dietary vitamin E level. This information may contribute to understanding the interactive effect of ginseng and vitamin E on cholesterol biosynthesis in high cholesterol-fed rats.

• Journal of Nutrition and Health
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• v.9 no.2
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• pp.24-39
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• 1976

The present study was undertaken to investigate the metabolic problems of high carbohydrate and cellulose diets of Korean. Forty males and same number of females of Albino rats, aged $45{\pm}5$days were divided into 95% high carbohydrate (H. CHO)group, 83.8% medium carbohydrate (M. CHO)group, 50% low carbohydrate (L. CHO) group and standard (Stand). group containing 72.2% sugar. Each group was divided into two again-1.55% cellulose group and non-cellulose group, 10 rats each of eight groups in both sexes. Cellulose was added to each of non-cellulose diets in the forms of spinach powder and rice bran. After 14 weeks the rats were sacrificed for chemical analysis and the results were elucidated as follows. (1) H. CHO+Cell. group showed the lowest value in body weight gained and shrinkage of almost all organs, in contrast with this group the L.CHO group showed higher body weight gained than Stand. group. M. CHO+Cell, group showed much the same body weight gained curve as Stand. group. (2) It was observed that cellulose group showed lower F.E.R and P.E.R value than non-cellulose group comparatively. (3) Total nitrogen retention and retention rate were decreaced in H. CHP groups compared with M. CHO or L. CHO groups. (4) The amount of feces was increased due to addition of cellulose to experimental diets and in accordance with the increasing total fecal excretion of nitrogen and glucose was also increased, especially noticeable in fecal glucose excretion. (5) It was noteworthy that serum cholesterol level was decreased due to addition of cellulose in H. CHO group and L. CHO group. (6) M. CHO+Cell. group was designed to reflect the average survey data of Korean diets and there was no significant differences on body weight gained, F.E.R, P.E.R, total nitrogen retention and hematology between M. CHO+Cell. and Stand. group. Total glucose excretion was increased due to dietary cellulose in M. CHO+Cell. group, but it seemed to be no metabolic problems in this group.

• Preventive Nutrition and Food Science
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• v.16 no.3
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• pp.202-209
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• 2011

This study was initiated to investigate the effects of lyophilized purslane (Portulaca oleracea) powder (5% and 10%) on lipid levels in rats fed a hypercholesterolemia-inducing diet (1% cholesterol). During the four week study, there were no significant differences in either weight change or the food efficiency ratio between the group fed the hypercholesterol diet alone (HC) and the groups fed the purslane powder (HCPO-I and HCPO-II, 5% and 10% purslane, respectively). In serum, the levels of total lipid, total cholesterol and LDL-C decreased significantly for the group fed the 10% purslane powder (HCPO-II) in comparison with the group fed the 5% purslane powder (HCPO-I). The atherogenic index (AI) was reduced by about 51% for the group fed the 10% purslane powder (1.47) in comparison with the HC group (3.03). The activities of GOT, GPT, ALP and LDH decreased significantly for the groups fed the purslane powder in comparison with the HC group. Regarding liver tissue, the levels of total lipid, total cholesterol and triglyceride decreased significantly for the purslane powder-fed rats compared to the HC group. The fecal lipid profiles increased significantly as the amount of purslane powder was increased. Compared to the HC group, the fecal total cholesterol and triglyceride levels were higher in the group fed the 10% purslane powder by about 2.8 times and 2.3 times, respectively. For the serum and liver tissue, the content of lipid peroxide decreased significantly in the groups fed purslane powder compared to the HC group. The data from this experiment show an increase in the lipid levels discharged in feces, suggesting that the supplementation of purslane powder to a hypercholesterolemia-inducing diet reduces lipid levels.

• Journal of Korean Society of Water and Wastewater
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• v.19 no.2
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• pp.149-154
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• 2005

Conventionally used flocculation tanks require large space and high energy requirement for mixing. Static flocculators using gravel bed filter operate at a lower flow rate ($5-10m^3/m^2{\cdot}h$). Further, the cleaning of this system is difficult. A novel high rate static flocculator/filter developed at UTS packed with buoyant media such as polystyrene, polypropylene has been found to operate at higher filtration rates (30-45 $5-10m^3/m^2{\cdot}h$). They can easily be cleaned with minimal energy. Detailed experiments conducted with an artificial kaolin clay solution show that buoyant media is an excellent static flocculator in producing uniform filterable microflocs (12-15 m) even when it is operated at a high rate of 30-40 m/h. Detailed filtration experiments were conducted in a wastewater treatment plant to treat the biologically treated effluent with a floating media of depth of 120 cm. This filter was able to remove majority of phosphorus and remaining solids. It reduced significantly the fecal coliforms and fecal streptoccoci, thus requiring less amount of chlorine for disinfection. The advantage of this system is the low energy and water requirement for cleaning of filter bed. The periodic backwash adopted 30 seconds air and water and 30 seconds water cleaning every 90 minutes filter operation. Thisis equivalent to 1-2% of filtered water production. Mechanical cleaning system on the other hand, requires very low energy requirement (<1% of filtered water production).

• Annals of Surgical Treatment and Research
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• v.95 no.6
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• pp.319-323
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• 2018

Purpose: Currently, many operations are performed using the single-incision laparoscopic method. Although there have been recent reports on single-incision laparoscopic ileostomy, none have compared this method to conventional laparoscopic ileostomy. This study aimed to assess the safety and feasibility of single-incision laparoscopic ileostomy for anastomotic leakage following laparoscopic low anterior resections. Methods: From April 2012 to April 2017, 38 patients underwent laparoscopic ileostomy (single-incision; 19 patients referred to as group A, conventional laparoscopy; 19 patients referred to as group B) for anastomotic leakage following laparoscopic low anterior resection. We analyzed surgical and clinical outcomes between the 2 groups. Patients in whom a protective ileostomy was carried out during the initial laparoscopic low anterior resection were excluded from this study. Results: No significant differences were observed between the 2 groups in terms of patient demographics and initial operation details. Incisional surgical site infections occurred less in group A than in group B (2 of 19 vs. 9 of 19, P = 0.029). The median ileostomy operation time, amount of intraoperative bleeding, parastomal hernia ratio, hospital stay duration after ileostomy, postoperative pain score were not significantly different between the 2 groups. Conclusion: Single-incision laparoscopic ileostomy is safe and feasible method of fecal diversion for anastomotic leakage following laparoscopic low anterior resection.