• Proceedings of the IEEK Conference
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• 2005.11a
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• pp.367-370
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• 2005

This paper proposes a novel method for content-based watermarking based on feature points of an image. At each feature point, watermark is embedded after affine normalization according to the local characteristic scale and orientation. The characteristic scale is the scale at which the normalized scale-space representation of an image attains a maximum value, and the characteristic orientation is the angle of the principal axis of an image. By binding watermarking with the local characteristics of an image, resilience against affine transformations can be obtained. Experimental results show that the proposed method is robust against various image processing steps including affine transformations, cropping, filtering, and JPEG compression.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.11 no.3
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• pp.75-81
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• 2011

Protein molecules are combined with another ones which have similar shapes at pocket positions. The pocket positions can be good references to describe the shapes of protein molecules. Harris corner detector is commonly used to detect feature points of 2 or 3D objects. Feature points can be found on the pocket areas and the points which have high derivatives. Generally speaking, the densities of feature points are relatively high at pocket areas because the shapes of pockets are concave. The pocket areas can be decided by the subdivision of voxel cubes which include feature points. The Euclidean distances between feature points and the central coordinate of the decided pocket area are calculated and sorted. The graph of sorted distances describes the shape of a protein molecule and the distribution of feature points. Therefore, it can be used to classify protein molecules by their shapes. Even though the shapes of protein molecules have been distorted with noises, they can be recognized with the accuracy more than 95 %. The accurate shape recognition provides the information to predict the binding properties of protein molecules.

• Journal of the Korean Magnetic Resonance Society
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• v.21 no.1
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• pp.13-19
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• 2017

Human sterol ${\Delta}8-{\Delta}7$ isomerase, commonly known as emopamil binding protein (EBP), is an essential protein in the cholesterol-synthetic pathway, and mutations of this protein are critically associated with human diseases such as Conradi-Hunermann-Happle or male EBP disorder with neurological defects syndrome. Due to such a clinical importance, EBP has been intensively investigated and some important features have been reported. EBP is a tetra-spanning membrane protein, of which $2^{nd}$, $3^{rd}$, and $4^{th}$ membrane-spanning ${\alpha}$ helices play an important role in its enzymatic function. However, detailed structural feature at atomic resolution has not yet been elucidated, due to characteristic difficulties in dealing with membrane protein. Here, we over-expressed EBP using Escherichia coli and performed detergent screening to find suitable membrane mimetics for structural studies of the protein by NMR. As results, DPC and LMPG could be evaluated as the most favorable detergents to acquire promising NMR spectra for structural study of EBP.

• Bulletin of the Korean Chemical Society
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• v.31 no.3
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• pp.630-634
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• 2010

The synthesis and ion binding properties of calix[4]pyrrole bearing pyrene moieties appended to one side of the calix[4]pyrrole are reported. The key feature is the presence of flexible fluorescence arms attached to the calix[4]pyrrole ring in a cis-fashion. The preliminary solution phase anion and cation binding studies revealed that the systems can be in fact as viable sensors for anionic guest.

• Journal of Microbiology
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• v.33 no.2
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• pp.128-131
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• 1995

Using differential hybridization, we selected the prk gene fortuitously from Schizosaccharomyces pombe homologous to RACK1 of rat which encodes the receptor for activated protein kinase C. The cDNA sequence of prk was determined and its deduced amino acid sequence was 76% homologous to RACK1 and had the feature of trimeric G protein bata subunit. The specific amino acid sequences required for the protein kinase C binding were also present in Prk as in the case of RACK1 protein. From these similarities, we suggest that the Prk is protein kinase C binding protein of S. prombe. The involvement of Prk in signal transduction mediated by protein kinase C remained to be studied.

• Journal of the Korean Magnetic Resonance Society
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• v.5 no.1
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• pp.29-36
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• 2001

The structures of two VBS (viral binding site) RNAs, SL1 and SL2, of Yeast Saccharomyces cerevisiae vims have been studied by 2D and 3D NMR experiments. VBSs play a crucial role in viral particle binding to the plus strand and packaging of the RNA. The secondary structures of the two VBS RNAs share a common feature of the stem-internal loop-stem-hairpin loop structure although the size of the internal loops of SL1 and SL2 differs. 2D experiments were sufficient for fill assignments of SL1. However, isotope labeling of the sample and multidimensional experiments were required for 28-nucleotide-long SL2 due to the spectral overlap. Several 3D HCCH experiments have accomplished full assignment of SL2 RNA.

• Quantitative Bio-Science
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• v.37 no.2
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• pp.113-124
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• 2018

Pim kinases are important targets for cancer therapies because they are mainly responsible for cancer metastasis and overall therapeutic treatment responses. Because of their unusual structural feature in the hinge region of the ATP-binding site, new binding motifs have been discovered and used for the development of Pim kinases inhibitors. The results of a screening of 5-membered heteroaromatic compounds and the effects of structural modifications on the inhibition of Pim kinases' activities showed the potential scaffold for Pim inhibitors. 1,3,4-Oxadiazole-2(3H)-thione was found as a new scaffold for Pim kinase inhibitors.

• KIPS Transactions on Software and Data Engineering
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• v.2 no.8
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• pp.571-578
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• 2013

The IHO S-100 is a standard on the universal hydorgraphic data model for supporting information services that integrate various data in maritime and provide proper information for safety of vessels. The S-100 is used to develop S-10x product specifications which are standards on guideline for creation and delivery of specific data set in maritime. The product specification for feature-based data such as ENC(Electronic Navigational Chart) data includes a feature catalogue that describes characteristics of features in that feature-based data. The feature catalogue is developed by domain experts with knowledge on data of the target domain. However, it is not feasible to develop a feature catalogue according to the XML schema by manual. In the IHO TSMAD committee meeting, needs of developing technology on building feature catalogue has been discussed. Therefore, we present a feature catalogue builder that is a GUI(Graphic User Interface) system supporting domain experts to build feature catalogues in XML. The feature catalogue builder is developed to connect with the FCD(Feature Concept Dictionary) register in the IHO(International Hydrographic Organization) GI(Geographic Information) registry. Also, it supports domain experts to select proper feature items based on the relationships between register items.

• Korean Journal of Cognitive Science
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• v.27 no.3
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• pp.355-376
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• 2016

We used holes defined by color similarity (Experiment 1) and binocular disparity (Experiment 2) to study how the inner contour of an object (i.e., boundary of a hole in it) is encoded in visual working memory. Many studies in VWM have shown that an object's boundary properties can be integrated with its surface properties via their shared spatial location, yielding an object-based encoding benefit. However, encoding of the hole contours has rarely been tested. We presented objects (squares or circles) containing a bar under a change detection paradigm, and relevant features to be remembered were the color of objects and the orientation of bars (or holes). If the contour of a hole belongs to the surrounding object rather than to the hole itself, the object-based feature binding hypothesis predicts that the shape of it can be integrated with color of an outer object, via their shared spatial location. Thus, in the hole display, change detection performance was expected to better than in the conjunction display where orientation and color features to be remembered were assigned to different parts of a conjunction object, and comparable to that in a single bar display where both orientation and color were assigned into a single bar. However, the results revealed that performance in the hole display did not differ from that in the conjunction display. This suggests that the shape of holes is not automatically encoded together with the surface properties of the outer object via object-based feature binding, but encoded independently from the surrounding object.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.63.2-63.2
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• 2015

Controlling and sensing spin states of magnetic molecules such as metallo-porphyrins at the single molecule level is essential for spintronic molecular device applications. Axial coordinations of diatomic molecules to metallo-porphyrins also play key roles in dynamic processes of biological functions such as blood pressure control and immune response. However, probing such reactions at the single molecule level to understand their physical mechanisms has been rarely performed. Here we present on our single molecule association and dissociation experiments between diatomic and metallo-porphyrin molecules on Au(111) describing its adsorption structures, spin states, and dissociation mechanisms. We observed bright ring shapes in NO adsorbed metallo-porphyrin compelxes and explained them by considering tilted binding and precession motion of NO. Before NO exposure, Co-porphryin showed a clear zero-bias peak in scanning tunneling spectroscopy, a signature of Kondo effect in STS, whereas after NO exposures it formed a molecular complex, NO-Co-porphyrin, that did not show any zero-bias feature implying that the Kondo effect was switched off by binding of NO. Under tunneling junctions of scanning tunneling microscope, both positive and negative energy pulses. From the observed power law relations between dissociation rate and tunneling current, we argue that the dissociations were inelastically induced with molecular orbital resonances. Our study shows that single molecule association and dissociation can be used to probe spin states and reaction mechanisms in a variety of axial coordination between small molecules and metallo-porphyrins.