• Journal of Life Science
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• v.17 no.5 s.85
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• pp.723-727
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• 2007

Resistance to metronidazole in Helicobacter pylori results from inactivation of rdxA and frxA, the chromosomal genes for a nitroreductase that normally converts metronidazole from prodrug to bactericidal agent. Two types of metronidazole susceptible strains had been found distinguishable by their apparent levels of frxA expression. Most common in the populations we had studied were strains that required only rdxA inactivation to become resistant to moderate levels of metronidazole(type I strains). The second strain type required inactivation of both frxA and rdxA to become resistance to metronidazole(type II strains): this was linked to a relatively high level of frxA gene transcription in the type II strains. The fdxA gene regulated fdxA as well as rdxA gene. Thus, to study the function of fdxA as a regulatory gene we constructed a null mutant of fdxA in H. pylori genome and identified over-and under-expressed proteins by fdxA using two-dimensional(2-D) electrophoresis and MALDI-TOP-MS. There were four over-expressed proteins in fdxA mutant; nifU-like protein(HP0221), frxA(HP0642), nonheme ferritin(HP0653), and hypothetical protein(HP0902). Three under-expressed proteins were also identified in fdxA mutant, including 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (HP0089), (3R)-hydroxymyristoyl ACP dehydratase(HP1376), and thioredoxin(HP1458).

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2020.07a
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• pp.579-581
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• 2020

최근 DOCSIS 3.1 시스템에서 주파수 이용효율향상을 위해 상향과 하향 신호를 동일대역으로 동시전송하는 DOCSIS3.1 FDX 시스템이 연구되고 있다. 동일대역을 사용할 경우 DOCSIS 3.1 FDX시스템의 하향 송신신호는 높은 전력을 가진 신호로 feedback되어 상향송신기로부터 전송된 낮은 전력의 상향 수신신호와 결합하여 상향수신기에 수신되어진다. 이러한 결합신호는 상향신호와 하향신호간의 심볼타이밍 옵셋이 정확하게 일치하지 않으면 상향신호 성능을 열화시키는 원인이 된다. 본 논문에서는 이러한 문제를 해결하기 위해 주파수영역에서 DOCSIS 3.1 FDX시스템의 상하향신호간 심볼 타이밍 옵셋으로 인한 성능 열화 개선 알고리즘을 제시하고 모의실험을 통해 성능열화를 개선할 수 있음을 보인다.

• Journal of Microbiology and Biotechnology
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• v.29 no.1
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• pp.44-54
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• 2019

Geldanamycin and its derivatives, inhibitors of heat shock protein 90, are considered potent anticancer drugs, although their biosynthetic pathways have not yet been fully elucidated. The key step of conversion of 4,5-dihydrogeldanamycin to geldanamycin was expected to catalyze by a P450 monooxygenase, Gel16. The adequate bioconversions by cytochrome P450 mostly rely upon its interaction with redox partners. Several ferredoxin and ferredoxin reductases are available in the genome of certain organisms, but only a few suitable partners can operate in full efficiency. In this study, we have expressed cytochrome P450 gel16 in Escherichia coli and performed an in vitro assay using 4,5-dihydrogeldanamycin as a substrate. We demonstrated that the in silico method can be applicable for the efficient mining of convenient endogenous redox partners (9 ferredoxins and 6 ferredoxin reductases) against CYP Gel16 from Streptomyces hygroscopicus. The distances for ligand FDX4-FDR6 were found to be $9.384{\AA}$. Similarly, the binding energy between Gel16-FDX4 and FDX4-FDR6 were -611.88 kcal/mol and -834.48 kcal/mol, respectively, suggesting the lowest distance and binding energy rather than other redox partners. These findings suggest that the best redox partners of Gel16 could be NADPH ${\rightarrow}$ FDR6 ${\rightarrow}$ FDX4 ${\rightarrow}$ Gel16.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2018.06a
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• pp.31-34
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• 2018

최근 유무선 트래픽의 지속적인 증가에 대응하기 위해선 가입자 네트워크의 양적 확충은 물론, 새로운 인터넷 기술을 개발하고 그 구조를 재설계하는 등의 투자가 이루어지고 있다. 특히 차세대 미디어 서비스 등의 인터넷 수요 및 대역폭에 대한 요구가 증가로 인하여 가입자 망 진화의 필요성이 계속해서 이야기되고 있다. 정부에서는 10기가 인터넷 상용화를 목표로 '10기가 인터넷 상용화 촉진 선도 시범 사업'을 추진하고 있으며, KT와 SK브로드밴드 등 주요 통신사에서도 10Gbps 인터넷 상용화를 추진하고 있다. 이에 케이블 사업자도 인터넷 경쟁력 확보를 위해 HFC망 진화 방안에 대한 연구가 필요하다. 우선 상하향 10Gbps 전송이 가능한 DOCSIS 3.1 도입을 위해 망 장비 교체나 업그레이드 없이 주파수 확보가 이루어 져야 하며, 상향 전송 속도 확대를 위해 북미에서 표준화 진행중인 FDX(Full-Duplex) DOCSIS 도입을 위해 망 구조를 개선해야 한다.

• Journal of the Korean Solar Energy Society
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• v.32 no.spc3
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• pp.282-288
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• 2012

Proton exchange membrane fuel cell (PEMFC) is the most promising energy source for the robot applications because it has unique advantages such as high energy density, no power drop during operating, and easy to make compact size. However, PEMFC has intrinsic disadvantages which are delay to start up and difficulty to correspond drastic load changes. These disadvantages can be compensated by hybrid operating with a Li-poly battery. This study is focus to build and understand the hybrid system for the robot system. In this study, we build the PEMFC hybrid system using EOS-320 PEMFC stack, Li-poly battery and G-Philos FDX1-250BU dc-dc converter. The hybrid system is accurately monitored by CAN and RS485. The system was studied under two conditions such as non-loaded and loaded operating conditions. The results show that the system has delay to start up without hybrid operating and it can be compensated with the hybrid operating.

• 한국태양에너지학회:학술대회논문집
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• 2012.03a
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• pp.229-233
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• 2012

Proton exchange membrane fuel cell (PEMFC) is the most promising energy source for the robot applications because it has unique advantages such as high energy density, no power drop during operating, and easy to make compact size. However, PEMFC has intrinsic disadvantages which are delay to start up and difficulty to correspond drastic load changes. These disadvantages can be compensated by hybrid operating with a Li-poly battery. This study is focus to build and understand the hybrid system for the robot system. In this study, we build the PEMFC hybrid system using EOS-320 PEMFC stack, Li-poly battery and G-Philos FDX1-250BU dc-dc converter. The hybrid system is accurately monitored by CAN and RS485. The system was studied under two conditions such as non-loaded and loaded operating conditions. The results show that the system has delay to start up without hybrid operating and it can be compensated with the hybrid operating.

• Journal of Microbiology and Biotechnology
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• v.32 no.9
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• pp.1110-1119
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• 2022

Fe-S clusters are versatile and essential cofactors that participate in multiple and fundamental biological processes. In Escherichia coli, the biogenesis of these cofactors requires either the housekeeping Isc pathway, or the stress-induced Suf pathway which plays a general role under conditions of oxidative stress or iron limitation. In the present work, the Fe-S cluster assembly Isc and Suf systems of acidophilic Bacteria and Archaea, which thrive in highly oxidative environments, were studied. This analysis revealed that acidophilic microorganisms have a complete set of genes encoding for a single system (either Suf or Isc). In acidophilic Proteobacteria and Nitrospirae, a complete set of isc genes (iscRSUAX-hscBA-fdx), but not genes coding for the Suf system, was detected. The activity of the Isc system was studied in Leptospirillum sp. CF-1 (Nitrospirae). RT-PCR experiments showed that eight candidate genes were co-transcribed and conform the isc operon in this strain. Additionally, RT-qPCR assays showed that the expression of the iscS gene was significantly up-regulated in cells exposed to oxidative stress imposed by 260 mM Fe₂(SO₄)₃ for 1 h or iron starvation for 3 h. The activity of cysteine desulfurase (IscS) in CF-1 cell extracts was also upregulated under such conditions. Thus, the Isc system from Leptospirillum sp. CF-1 seems to play an active role in stressful environments. These results contribute to a better understanding of the distribution and role of Fe-S cluster protein biogenesis systems in organisms that thrive in extreme environmental conditions.

• Journal of Aerospace System Engineering
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• v.16 no.1
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• pp.1-11
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• 2022

Avionics Full-DupleX Ethernet (AFDX) is a next-generation avionics network interface technology that is widely applied in the latest aircraft to replace ARINC429 and MIL-STD-1553B. However, the criteria for authenticating an avionics network consisting of AFDX are very scarce. Using AFDX Protocol Analyzer developed by the Korea Electronics Technology Research Institute and AFDX Tap developed by the Korea Aerospace University, we proposed a technology of certification practicality that can verify the normal functioning of avionics equipment with AFDX network interface. Our proposed technology provided the ability to collect precision packets, to verify AFDX specification compliance, and perform automatic tests to reduce the time and cost of authentication of AFDX avionics devices.