• Title/Summary/Keyword: fatty acid.

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Studies on the Contents of Special Components and Estimation of Purity of Sesame Oil II (참기름의 특이 성분 함량과 순도 결정에 관한 연구(제2보))

  • 노일협;임미애
    • YAKHAK HOEJI
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    • v.27 no.2
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    • pp.169-176
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    • 1983
  • The verification of genuine sesame oil can be examined by determination of the ratio of fatty acid. Fatty acids were extracted from the saponifiable substance of sesame oils. Fatty acids were methylated with the 14% boron trifluoride methanol solution and injected into a gas chromatograph with Unisole 3000 column and finally determined the molecular weight by mass spectrometry. The fatty acids in laboratory prepared sesame oils were composed mainly of oleic acid 36.7-42.8% and linoleic acid 39.0-46.6%, including palmitic acid 7.9-9.l%, stearic acid 4.1-5.6%, linoleic acid 0.1-3.0%, arachidic acid 0.5-1.0% and eicosenoic acid 0.1-0.5%. The above results allow the estimation of genuine sesame oil, mixed with rape seed oil, soybean oil, perilla oil, etc. In 53 samples, 14 samples were estimated as genuine and it was found that erucic acid was contained in 31 samples, linoeic acid was highly contained in 14, high quantity of linolenic acid was in 7 and palmitic and oleic acid were highly involved in 3.

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Effect of Dietary n-3 Fatty Acids and Fat Unsaturation on Plasma Lipids and Lipoproteins in Rats (식이의 n-3 지방산과 지방의 불포화도가 혈장지질 조성에 미치는 영향)

  • 박현서
    • Journal of Nutrition and Health
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    • v.25 no.7
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    • pp.555-568
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    • 1992
  • This study was to compare the effects of dietary n-6 and n-3 fatty acids and fat unsaturation on plasma lipids and chemical composition of VLDL and LDL fraction and lipogenic enzymes activity in rat liver under the conditions providing 1) a similar amount of n-6, n-3 fatty acids(LA, ALA, EPA+DHA) in diets and 2) the various degree of fat unsaturation. Male Sprague-Dawley rats weighing 420g were treated for 6-n with six experimental diets providing 25% of energy as fat and which were different only in fatty acid composition. The fats used for a source of each fatty acid were beet tallow for saturated fatty acid corn oil for n-6 linoleic acid(LA) perilla oil for n-3 $\alpha$-linolenic acid(ALA) and fish oil n-3 eicosapentaenoic acid (EPA) and n-3 docosahexaenoic acid(DHA). Plasma cholesterol level was increased by corn oil to compare with beef tallow but was decreased by perilla oil or fish oil. Plasma TG level was significantly decreased by perilla oil or fish oil. Fish oil significantly reduced the level of HDL-Chol and the proportion of Chol in LDL fraction and that of TG in vVLDL fraction. Overall there was a singificant negative correlation between the level of each plasma lipid(Chol TG, VLDL-TG, LDL-C) and the degree of fat unsaturation. However this rerlationship is not always true when compared the hypolipidemic effect of each fatty acid at a similar level of fat unsaturation. There was a trend such taht glucose 6-P dehydrogenase 6-phosphogluconate dehydrogenase and malic enzyme activites were reduced by n-3 fatty acids. Perilla oil significantly increased the incorporation of c20:5 and c22:5 into liver tissue and fish oil suignificantly increased the incorporation of c20:5, c22:6 into liver tissue and the effect of long chain n-3 fatty acid incorporation was greater by fish oil. therefore the hypotriglyceridemic effect of n-3 fatty acid could be resulted from the interference of hepatic lipogenesis by long-chain n-3 fatty acids and the reduced proportion of TG in VLDL fraction and its effect was greater by n-3 EPA+DHA than n-3 ALA even though plasma Chol and TG levels were also influenced by the degree of dietary fat unsaturation.

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Study on the fatty acid profile of phospholipid and neutral lipid in Hanwoo beef and their relationship to genetic variation

  • Beak, Seok-Hyeon;Lee, Yoonseok;Lee, Eun Bi;Kim, Kyoung Hoon;Kim, Jong Geun;Bok, Jin Duck;Kang, Sang-Kee
    • Journal of Animal Science and Technology
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    • v.61 no.2
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    • pp.69-76
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    • 2019
  • Maize which has very high omega-6 fatty acid content has been used as a main feed grain for Hanwoo beef production to increase marbling, and thus omega-6 to omega-3 fatty acids ratio in Hanwoo beef is expected to be biased. To elucidate the current status of omega fatty acids ratio in Hanwoo beef, fatty acid profiles of neutral lipid and phospholipid fraction were analyzed separately using 55 Hanwoo steers' longissimus dorsi muscle slaughtered at Pyeongchang, Korea from Oct. to Nov. 2015. In addition, an association study was conducted to evaluate associations between single nucleotide polymorphism (SNP) markers from references and omega fatty acid profiles in phospholipid of Hanwoo beef samples using analysis of variance (ANOVA). In neutral lipid fraction, composition of saturated and monounsaturated fatty acids was higher and polyunsaturated fatty acids was lower compared to those in phospholipid fraction. The mean n-6/n-3 ratios of Hanwoo were $56.059{\pm}16.180$ and $26.811{\pm}6.668$ in phospholipid and neutral lipid, respectively. There were three SNPs showing statistically significant associations with omega fatty acid content. GA type of rs41919985 in fatty acid synthase (FASN) was significantly associated with the highest amount of C20:5 n-3 (p = 0.031). CC type of rs41729173 in fatty acid-binding protein 4 (FABP4) was significantly associated with the lowest amount of C22:2n-6 (p = 0.047). AG type of rs42187261 in FADS1 was significantly linked to the lowest concentration of C20:4 n-6 (p = 0.044). The total n-6/n-3 ratio of the steer which has all four SNP types in above loci (27.905) was much lower than the mean value of the total n-6/n-3 ratio in phospholipid of the 55 Hanwoo steers ($56.059{\pm}16.180$). It was found that phospholipid and neutral lipid of Hanwoo have very high n-6/n-3 ratios compared to the reported data from different cow breeds. Four SNPs in genes related with fatty acid metabolism showed significant associations with the fatty acid profile of phospholipid and may have potential as SNP markers to select Hanwoo steers in terms of n-6/n-3 balance in the future.

Effects of Cooking and Drying Methods on the Lipid Content and Neutral Lipid Composition of Shrimp (가열 및 건조방법이 새우의 지방질 함량과 중성지방질 조성에 미치는 영향)

  • Kim, Hyun-Ku;Hawer, Woo-Derck S.;Shin, Hyo-Sun
    • Korean Journal of Food Science and Technology
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    • v.21 no.1
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    • pp.17-24
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    • 1989
  • Effects of cooking and drying methods on the lipids content and neutral lipid component of shrimp, Metapenaeus joyneri, were investigated. Total lipid content of the fresh shrimp was 6.0%(dry basis), which was not changed significantly depending on the cooking and drying methods. Lipid of the fresh shrimp was composed of 36.8% of neutral lipids, 21.5% of glycolipids, and 41.7% of phospholipids. Freeze dried shrimp was substantially higher in neutral lipid contents but lower in glycolipid and phospholipid contents than hot air dried one. Main components of the neutral lipids were triglycerides, free sterols, free fatty acids and esterified sterols. Freeze dried shrimp contained higher triglycerides contents than hot air dried shrimp, whereas free fatty acids content in hot air dried shrimp was greater than that of freeze dried one due to the decomposition of triglycerides to free fatty acids during hot air drying. Major fatty acid composition of the total lipid were palmitoleic acid, eicosapentaenoic acid and docosahexaenoic acid, and that of neutral lipid were palmitic acid, palmitic acid, oleic acid, eicosapentaenoic acid and docosahexaenoic acid in fresh shrimp.

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The Composition of Useful Medium Chain Fatty Acids in Eight Plant Species (특이지방산의 탐색과 붓꽃류에서 중쇄지방산(myristic acid)의 확인)

  • Kim, Jong-Bum;Kim, Kyung-Hwan;Hwang, Sun-Kap;Kim, Yong-Hwan;Cho, Kang-Jin;Hwang, Young-Soo;Park, Ro-Dong;Kim, Jung-Bong
    • Applied Biological Chemistry
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    • v.44 no.1
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    • pp.20-23
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    • 2001
  • Several unusual fatty acids including myristic acid (14 : 0) and lauric acid (12 : 0) were investigated in the Iris family and other related plants. Especially the roots of Iris tecorum contained 75.9% myristic acid in total fatty acid contents and that of Iris germanica contained 57% myristic and 15.5% lauric acid (12 : 0) whereas 10.7% lauric acid and 9.5% capric acid (10 : 0) were detected in the roots of iris ensata as compared to the total fatty acid contents. The total fatty acid contents in the seeds of Foeniculi fructus and Torilis japonica was relatively higher 193.3 mg/g dry wt and 128.2 mg/g dry wt, respectively. 64.5% linoleic acid (18 : 2) and 48.9% ${\alpha}-linolenic$ acid (18 : 3) were observed in the seeds and leaves of iris tectorum whereas its lateral roots contained 9.5% caprylic acid (8 : 0) and 8.6% capric acid. The percentage of myristic acid of the total fatty acid in the immature seeds of iris tectorum and Iris germanica was 10.8% and 15.6%, respectively.

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Seamustard (Undaria pinnatifida) Improves Growth, Immunity, Fatty Acid Profile and Reduces Cholesterol in Hanwoo Steers

  • Hwang, J.A.;Islam, M.M.;Ahmed, S.T.;Mun, H.S.;Kim, G.M.;Kim, Y.J.;Yang, C.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.8
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    • pp.1114-1123
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    • 2014
  • The study was designed to evaluate the effect of 2% seamustard (Undaria pinnatifida) by-product (SW) on growth performance, immunity, carcass characteristics, cholesterol content and fatty acid profile in Hanwoo steers. A total of 20 Hanwoo steers (ave. 22 months old; 619 kg body weight) were randomly assigned to control (basal diet) and 2% SW supplemented diet. Dietary SW supplementation significantly (p<0.05) improved average daily gain and gain:feed ratio as well as serum immunoglobulin G concentration. Chemical composition and quality grade of meat and carcass yield grades evaluated at the end of the trial were found to be unaffected by SW supplementation. Dietary SW significantly reduced meat cholesterol concentration (p<0.05). Dietary SW supplementation significantly reduced the myristic acid (C14:0) and palmitoleic acid (C16:ln-7) concentration, while SW increased the concentration of stearic acid (C18:0) and linolenic acid (C18:3n-3) compared to control (p<0.05). Dietary SW supplementation had no effect on saturated fatty acids (SFA), unsaturated fatty acids, poly unsaturated fatty acid (PUFA) or mono unsaturated fatty acid content in muscles. A reduced ratio of PUFA/SFA and n-6/n-3 were found in SW supplemented group (p<0.05). In conclusion, 2% SW supplementation was found to improve growth, immunity and fatty acid profile with significantly reduced cholesterol of beef.

Isolation and Identification of Antifungal Fatty Acids from the Extract of Common Purslane(Portulaca oleracea L.) (쇠비름 즙액에서 얻은 항균성 지방산의 분리 및 동정)

  • Park Jong Seong;Nishimura Shoyo;Marumo Shingo;Katayama Masato
    • Korean Journal Plant Pathology
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    • v.2 no.2
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    • pp.82-88
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    • 1986
  • Five antifungal substances were isolated from the long-term storaged extract of common purslane, and identified as isobutyric, butyric, isovaleric, valerie and caproic acids belonging to short-chain fatty acids (C4­C6). Each of these fatty acids showed more or less antifungal potency against spore germination and mycelial growth of Alternaria alternata Japanese pear pathotype in vitro. Antifungal potency of each fatty acid against spore germination was greater than that against the mycelial growth. No one of these fatty acids completely inhibited the mycelial growth at concentration lower than 200 ppm, while 50 ppm of caproic acid and 200 ppm of valerie acid completely inhibited the spore germination. The results of bioassay also suggested that chain-length of the fatty acids might be related with the antifungal potency, since fatty acids with longer chain showed higher antifungal potency.

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Effect of Supplementing the Diet of Male Chickens With Oils Rich in n-6 Polyunsaturated Fatty Acids on the Fatty Acid Profiles of the Testis and Liver

  • Surai, Peter F.;Cerolini, Silvia;Speake, Brian K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.11
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    • pp.1518-1522
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    • 2000
  • Since the n-6 polyunsaturated fatty acid, docosatetraenoic acid (22:4n-6), is a major functional constituent of avian spermatozoa, the effects of two dietary oils rich in fatty acids which are metabolic precursors of 22:4n-6 on the fatty acid profiles of testicular lipids were investigated during a 39 week period of supplementation from 21 to 60 weeks of age. The effects on liver lipids were determined for comparison. Dietary supplementation of male chickens with Arasco Oil, which provides a large amount of arachidonic acid (20:4n-6), increased the proportion of 20:4n-6 in liver phospholipid by almost 2.5-fold. Although liver phospholipid normally contains very little 22:4n-6, this proportion was significantly increased as a result of Arasco feeding, indicating that the conversion of 20:4n-6 to 22:4n-6 was occurring. The phospholipid of the testis contains much higher proportions of 20:4n-6 and particularly of 22:4n-6 than the liver; supplementation with Arasco Oil significantly increased the proportions of both these polyunsaturates in testis phospholipid but the magnitude of this effect was much lower than that which occurred in the liver. Dietary supplementation with Evening Primrose Oil which contains ${\gamma}-linolenic $ acid (18:3n-6) resulted in significant increases in the proportions of 20:4n-6 and 22:4n-6 in liver phospholipid, although the extent of this increase was less than that produced by the Arasco Oil. By contrast, the feeding of Evening Primrose Oil did not alter the fatty acid composition of phospholipid in the testis. The findings raise the possibility that dietary supplementation with Arasco Oil may modulate the fatty acid profile of avian spermatozoa in a way which could potentially be beneficial for fertility. Moreover, the weights of the testes were almost doubled as a result of supplementation with Arasco Oil or Evening Primrose Oil.

Antifungal Activity of Medium-chain Saturated Fatty Acids and Their Inhibitory Activity to the Plasma Membrane H+-ATPase of Fungi (중급 지방산 항진균 활성과 진균의 Plasma membrane H+-ATPase에 대한 저해작용)

  • 이상화;김창진
    • Microbiology and Biotechnology Letters
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    • v.27 no.5
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    • pp.354-358
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    • 1999
  • In order to know the antifungal characteristics of saturated fatty acids having 6 to 12 carbons, their minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) were estimated against Saccharomyces cerevisiae. Fatty acids from C6 to C11 exhibited increasing activity with chain length, but C12 fatty acid did not show activity at all. In relation to antifungal modes of actions, fatty acids investigated showed on inhibitory activity toward the plasma membrane H+-ATPase of Saccharomyces cerevisiae. Their inhibitions to the glucose-induced acidification and ATP hydrolysis caused by the proton pump were found to be in common wiht antifungal activities. At the test concentration of 1mM, hexanoic acid (C6) showed the lowest inhibition of about 30%, while undecanoic acid(C11) showed the strongest inhibition of over 90%. In addition, as seen with antifungal activity, the inhibitory activity of dodecanoic acid (C12) was suddenly reduced to less than 50%.

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Influence of Kinds of Fatty Acids and Poly(ethylene glycol)s on Properties of Silica-Filled Natural Rubber Compounds

  • Park, Sung-Seen;Park, Sumgsoo
    • Macromolecular Research
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    • v.9 no.2
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    • pp.92-99
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    • 2001
  • Silica-filled rubber compounds have slower cure characteristics than carbon black-filled ones due to the adsorption of curatives on the silica surface. Fatty acid was used as a cure activator along with zinc oxide in a sulfur cure system. Poly(ethylene glycol), PEG, was used in silica-filled rubber compounds to prevent adsorption of the curatives on the silica surface. In this study, influence of the size of fatty acid and PEG on properties of silica-filled NR compounds was investigated. It was found that the size of fatty acid and PEG affected the curt: characteristics and physical properties. The cure rate becomes faster as the PEG size increases. By increasing the size of fatty acid or PEG, the delta torque of the compound decreases while the Mooney viscosity increases. The modulus of the vulcanizate decreases with increasing the molecular weight of fatty acid or PEG. The experimental results were explained by the filler dispersion and by the prevention of the curative-adsorption on the silica surface.

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