• Title/Summary/Keyword: fatty acid ester

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Effects of High Polyunsaturated Fat Diet on Human Plasma Cholesterol Esterification and Transfer (고 불포화 지방산 식이가 인체 혈청 Cholesterol의 Esterification과 Transfer에 미치는 영향)

  • Lee Myoung Sook
    • Journal of Nutrition and Health
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    • v.26 no.7
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    • pp.819-828
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    • 1993
  • The reaction of lecithin cholesterol acyltransferase(LCAT) and cholesteryl ester transfer protein (CETP) are important in cholesterol esterification and transfer for the reverse cholesterol transport(RCT) system. The purpose of this study were to assess the effects of fatty acid unsaturation on RCT system. After 12 female human subjects consumed a prescribed high saturated fat diet prior to the period, two groups of six subjects were provided with a high PUFA(corn oil) or a high SFA(butter) as major fats in a 40 en % fat diet. Butter feeding increased plasma total-(34%), esterified-(96%), HDL_3$-(23%), LDL-(20%), and VLDL plus LDL(35%) cholesterol while corn oil feeding decreased esterified-(25%), LDL(15%) cholesterol and TG(27%). There were significant differences of fats on total-(p=0.0001), esterified-(p=0.0001), total HDL-(p=0.005), $HDL_2$-(p=0.01)and LDL-(p=0.0001) cholesterol. LCAT activity did not change during the period but highly correlated to apo A-I in HDL which was increased in the corn oil group. The 2.5 fold increased CETP activity in the butter group during the period might be related to changes in plasma VLDL plus LDL cholesterol level which were increased in the butter group.

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Effect of Fire on Microbial Community Structure and Enzyme Activities in Forest Soil (산불이 토양 미생물 군집과 효소 활성 변화에 미치는 영향)

  • Oh, Ju-Hwan;Lee, Seul-Bi;Park, Sung-Eun;Lee, Yong-Bok;Kim, Pil-Joo
    • Korean Journal of Environmental Agriculture
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    • v.27 no.2
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    • pp.133-138
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    • 2008
  • Fire can affect microbial community structure of soil through altered environmental conditions, nutrient availability, and biotic source for microbial re-colonization. We examined the influence of fire on chemical properties and soil enzyme activities of soil for 10 months. We also characterized the soil microbial community structure through ester-linked fatty acid analysis(EL-FAME). For this study, we established five burned plots(1*1 m) and 5 unburned plots outside the margin of fire. Soil was sampled three soil cores in a each plots and composited for analysis at 1, 3, 5, 8, and 10 month after fire. The fire caused an increase in soil pH, exchangeable Ca, and Mg, organic matter, available $P_2O_5$ compared to unburned sites. The content of $NH_4-N$ in burned site was significantly higher than that of unburned site and this effect continued for 8 months after fire. There was no difference of $NO_3-N$ content in soil between burned and unburned site. Fire caused no change in acid phosphatase and arylsulfatase activities but $\beta$-glucosidase and alkaline phosphatase activities in burned site were increased compared to unburned site. Microbial biomass as estimated by total concentration of EL-FAMEs in burned sites was significantly higher than that of unburned sites at one month after fire. Burned site decreased the EL-FAMEs indicative of gram-positive bacteria and tended to increase the fatty acid associated with gram-negative bacteria at one and three months after fire. The sum of EL-FAME compound $18:2{\omega}6,9c$ and $18:1{\omega}9c$ as served fungal biomarkers was decreased in burned site compared to unburned site.

Changes in Chemical Component and Lipid in Tomato Fruits Under the Treatment of Sub-Atmospheric Pressure (감압처리(減壓處理)에 따른 토마토과실(果實)의 주요성분(主要成分) 및 함유지질(含有脂質)의 변화(變化))

  • Kim, Sung-Dal
    • Applied Biological Chemistry
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    • v.23 no.1
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    • pp.31-44
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    • 1980
  • Climacteric phenomon in fruits was delayed ten days on the sub-atmospheric treatment than the normal atmospheric one. Average lipid content in tomatoes sampled was composed of neutral lipid (49%), phospholipid(31%) and glycolipid (13%). Seven constituents each from neutral and phospholipid were seperated through thin-lyaer chromatography. Changes on lipid content during treatments were associated with the climacteric pattern and the advance of maturity; At the climacteric on-set, the fruit color was light pink, and the content of neutral lipid was reduced to minimum. After this stage, this content was reversely increased. The maturity was related with diglyceride and sterol ester in neutral lipid, and phosphatidyl choline and phosphatidyl ethanolamine in phospholipid. The changes of two constituents each on both neutral and phospholipid were reversed each other. It was concluded that changes of lipid content in tomato fruits were closely associated with the advance of fruit maturity and climacteric pattern.

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Volatile Components of Traditional Gochujang Produced from Small Farms according to Each Cultivation Region (지역별 소규모 농가 생산 전통 고추장의 휘발성 성분에 관한 연구)

  • Hong, Yeo Joo;Son, Seong Hye;Kim, Ha Youn;Hwang, In Guk;Yoo, Seung Seok
    • Journal of the East Asian Society of Dietary Life
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    • v.23 no.4
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    • pp.451-460
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    • 2013
  • The purpose of this study is to investigate the volatile compounds of Korean traditional gochujang from various districts. The volatiles from each traditional gochujang are being extracted by simultaneous steam distillation extraction (SDE), and analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Twenty compounds are identified as major volatile components which include 8 esters, 4 alcohols and 4 acids. The most traditional gochujang possesses more volatile components rather than commercial gochujang products. Most acids come from fatty acids and the alcohols derive from the oxidative degradation of linolenic acid. The most abundant volatile compounds for both traditional and commercial gochujang include 10 compounds such as 2-methyl-1-propanol, hexanal, 2-methyl-1-butanol, octanoic acid ethyl ester, as well as the various type of acids and esters. They represent most of the total GC peak areas, respectively. From the results, the characteristics of the flavors for traditional gochujang from each district are not clear but have shown various components than the commercial products.

Purification and Characterization of a Carotenoprotein from Penaeus orientalis (대하(P. orientalis)로부터 분리정제한 Carotenoprotein의 특성에 관한 연구)

  • Lee, Seo Gu;Kim, Jae Ung
    • Journal of the Korean Chemical Society
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    • v.38 no.8
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    • pp.608-615
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    • 1994
  • The isolation, purification and characterization of a carotenoprotein from the carapace of Pnaeus orientalis were investigated. The carotenoprotein was purple with broad λmax between 480, 409, 318 and 280 nm. Apparent structures were estimated by using X-ray diffractometry and scanning electron microscope, respectively. The molecular weight of the carotenoprotein complex had been determined by GPC and PAGE. The heavier complex, designated the $\alpha$-form (M.W = 170 KDa), was dissociated to a major subunit, $\beta$-form (M.W = 42 KDa). SDS-PAGE of $\alpha$-form showed apparently oligomeric pattern, and also $\beta$-form gave two polypeptides corresponding to 22 KDa and 19 KDa, respectively. The amino acid of the two proteins $({\alpha}-and\;{\beta})$-form, lipid and free fatty acid compositions were described. The prosthetic groups of the carotenoprotein were confirmed by TLC, IR, $^1H$-NMR, MS and various organic reactions as astaxanthin, astaxanthin monoester and astaxnathin diester.

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Effects of Turmeric (Curcuma longa L.) on Blood Glucose and Lipid Metabolism Functional Improvement in STZ-induced Diabetic rats (울금(Curcuma longa L.) 섭취가 당뇨성 흰쥐의 혈당 및 지질대사 기능 개선에 미치는 영향)

  • Oh, Da-Young;Kang, Dong-Soo;Lee, Young-Geun;Kim, Han-Soo
    • Journal of Environmental Science International
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    • v.28 no.5
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    • pp.485-494
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    • 2019
  • The purpose of this study was to investigate the improvement effect of 5% turmeric (Curcuma longa L.) on the blood glucose and lipid metabolism function of streptozotocin (STZ, 45 mg/kg body weight)-induced diabetic rats. Seven-week-old male rats were divided into four groups (n=6), and fed experimental diets containing turmeric meal [basal diet+5% turmeric (BT), basal diet+STZ+5% turmeric (ST)], and control (BD), BS groups (basal diet+STZ). The results of this study, turmeric diet groups (BT, ST) in lipid component as evidenced from the significantly reduction of serum total cholesterol, low density lipoprotein-cholesterol (LDL-cholesterol), atherosclerotic index (AI), cardiac risk factor (CRF), triglyceride (TG), phospholipid (PL), free cholesterol, cholesteryl ester, uric acid, blood glucose, non esterified fatty acid (NEFA), and elevation of high density lipoprotein-cholesterol (HDL-cholesterol). The serum albumin and globulin contents were increased in turmeric supplementation diet than STZ-induced diabetic rats (p<0.05). Concentrations of sodium (Na) and chlorine (Cl) in sera were lower in the turmeric diet than diabetic group. Total calcium (T-Ca), phosphorus (Pi) and potassium (K) concentrations in sera were higher in the BT, ST and BD groups than BS group. In vivo experiments with Sprague-Dawley rats showed that ingestion of turmeric (Curcuma longa L.) were effective in the blood glucose and lipid metabolism functional improvement.

Production of Lard Based Biodiesel Using Ultrasound Assisted Trans-Esterification (초음파가 도입된 전이에스테르화 반응을 이용한 돈지원료 바이오디젤의 제조)

  • Cho, Hae-Jin;Lee, Seung-Bum;Lee, Jae-Dong
    • Applied Chemistry for Engineering
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    • v.22 no.2
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    • pp.155-160
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    • 2011
  • An animal fat is an attractive biodiesel energy source for its high stability against oxidation and low incomplete combustion ratio due to the high heating value and cetane value. However, it requires a refinery process because of the high content of saturated acid and impurity which increas the boiling point. In this study, the optimum biodiesel synthetic process of lard is suggested. Indeed, we demonstrate new biodiesel production processes to alter conventional process of heating and mixing by applying ultrasonic energy. While the optimum reaction temperature and mole ratio of methanol and lard, when using conventional mixing and heating process, were $55^{\circ}C$ and 12, respectively, the reaction time were reduced to 30 minutes by applying ultrasonic irradiation power of 500 W. The new process applying ultrasonic irradiation yielded synthetic biodiesel properties as followings: 3.34 cP of the viscosity, 37.0 MJ/kg of the caloric value and below 0.25 mgKOH/g of the acidic value, which satisfy biodiesel quality criteria.

Food Components of Wild and Cultured Fresh Water Fishes (천연 및 양식산 담수어의 식품성분)

  • KIM Kyung-Sam;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.3
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    • pp.195-211
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    • 1986
  • The object of this study is to obtain fundamental data on cultured fishes produced in Korea to improve their food components. For this purpose, the food components of cultured fresh water fishes such as eel, Anguilla japonica, snakehead, Channa argus, and common carp, Cyprinus carpio, were investigated and compared with those of the wild ones. The results obtained are summarized as follows: 1. Common characteristics in the proximate composition were that wild fish was higher in crude protein content and lower in crude lipid content than those of cultured one. 2. Among the 9 kinds of minerals analyzed in all the samples, sodium, potassium, calcium and magnesium contents were absolutely predominant being more than $99.52\%$. These four elements in feedstuff also occupied $99.68{\sim}99.92%$ of total minerals. 3. The neutral lipids of wild and cultured eel, snakehead and common carp occupied $55.7{\sim}95.8%$ of lipid fractions, while the content of the phospholipids in snakehead was particularly higher than those of others. 4. The neutral lipids of wild and cultured eel, snakehead and common carp mainly consisted of triglycerides ($85{\sim}95%$), and a little quantity of diglycerides, monoglycerides, free sterol ester and hydrocarbon were also identified in the neutral lipid. 5. The phospolipids of eel and common carp were mainly occupied by phosphatidyl choline ($71.3{\sim}83.9%$), followed by phosphatidyl ethanolamine ($12.1{\sim}23.5%$) and phosphatidyl serine ($7.5{\sim}13.8%$). The phospholipids of snakhead consisted of phosphatidyl choline ($50.7{\sim}64.5%$), phosphatidyl ethanolamine ($28.0{\sim}35.5%$) and phosphatidyl serine ($7.5{\sim}13.8%$). Generally, phosphatidyl choline content was higher in wild fish than in cultured one, while phosphatidyl ethanolamine and phosphatidyl serine contents were higher in cultured one. 6. The major fatty acids in total lipid of wild eel, snakehead and common carp were $C_{16:0}\;and\;C_{20:5}$, while those in cultured ones were $C_{18:1},\;C_{18:2}\;and\;C_{22:6}$. The fatty acid composition of neutral lipids showed similar tendency to that of total lipid, and the main fatty acids in phospholipids of cultured fishes were $C_{18:1}\;and\;C_{18:2}$. In glycolipids, $C_{20:5}\;and\;C_{22:6}$ were higher in wild fishes, while $C_{18:2}$ were higher in cultured ones. 7. Total amino acids contents of wild and cultured eel were nearly the same, being $16.65\%$ ana $15.99\%$ respectively. The major amino acids of wild and cultured fish were glutamic acid, leucine, aspartic acid and lysine in order. In snakehead, the contents of aspartic acid and proline in cultured fish were higher than those in wild one, while the contents of glutamic acid, alanine, glycine were higher in the wild one. Total amino acid content of cultured common carp was $21.7\%$ compared with $17.08\%$ in wild one. The contents of glutamic acid, aspartic acid, glycine, proline and alanine occupied higher quantities in cultured common carp compared with those in wild one while the other amino acids revealed no significant difference. 8. Aspartic acid in free amino acids of cultured eel held $1.0\%$ of total free amino acids, while that in wild eel held $2.9\%$. Histidine, arginine and tyrosine content of cultured fish were two times higher than those of wild one. But free amino acid composition of samples seemed to be no marked differences according to cultured places. The contents of arginine, aspartic acid, glutamic acid, methionine and phenylalanine of snakehead ware higher in wild one than in cultured one, while the contents of lysine, histidine, glycine, and alanine ware higher in cultured one. In free amino acids content of wild common carp, histidine, glycine and lysine occupied $76.9\%$ of total free amino acids. Lysine, histidine, aspartic acid, alanine, valine and leucine were higher in wild one compared with those of cultured one, while glycine and tyrosine contents were higher in cultured fish.

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Effects of Feeding the Mixed Oils of Butter, Sardine and Safflower on the Lipid Components in Serum and Activities of Hepatic Functional Enzymes in Rats (버터, 정어리유 및 홍화유의 혼합 급이가 흰쥐의 혈청 지질성분 및 간기능 효소 활성에 미치는 영향)

  • Kim, Han-Soo;Chung, Seung-Yong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.6
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    • pp.608-616
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    • 1992
  • This study was designed to observe the effects of feeding the mixed oils of the sardine oil containing n-3 EPA, DHA and the safflower oil which is rich in n-6 linoleic acid on the improvement of the lipids and enzyme activities of serum in rats. Experimental oils mixed with 16% butter (control group) and 8% butter + 8% olive oil, 8% butter and various level of sardine and safflower oils were administered to the male rats of the Sprague Dawley for 4 weeks. The activities of aspartate aminotransferase (AST, EC 2.6.1.1), alanine aminotransferase (ALT, EC 2.6.1.2), lactate dehydrogenase (LDH, EC 1.1.1.27) and alkaline phosphatase (ALP, EC 3.1.3.1) in serum were significantly decreased in the all experimental groups than in the control groups, and activities of ALT and LDH were remarkably lower in the group 5 (4% sardine 0il + 4% safflower oil). Concentrations of total cholesterol and HDL-cholesterol in serum were lower in the other groups than in the dontrol groups, and particularly, lowest in the group 5. Concentrations of LDL, LDL-cholesterol, phospholipid and triglyceride in serum were lower in the all experimental groups than in the control group. Concentrations to total cholesterol and cholesteryl ester in serum were lowest in the group 5. The ratio of cholesteryl ester to total cholesterol was remarkably high in the control group, while group 2 (8% olive oil) was the lowest. From this results, the feeding equal quantity mixed oil with n-3 PUFA rich sardine oil and n-6 PUFA rich safflower oil were effective on the improvement of the lipid composition in the serum. It might be due to the effects of appropriate ratios of P/S, 0.85 and n-6/n-3P, 2.85 in the test lipids.

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Effect of Additive, Storage Temperature and Time on the Texture Properties of Baikseolgi (첨가물, 저장온도 및 저장시간에 따른 백설기의 텍스쳐 특성)

  • Kim, Myung-Hwan
    • Applied Biological Chemistry
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    • v.41 no.6
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    • pp.437-441
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    • 1998
  • The effects of sucrose fatty acid ester (SE, 1% w/w) and glycerin (GL, 1% v/w) additions, storage temperature$(0,\;20\;and\;70^{\circ}C)$, and time $(0{\sim}6\;day)$ on texture properties, hardness(H), cohesiveness(O), chewiness(C) and rheological property(R) of Baikseolgi were studied. The H of Baikseolgi increased sharply in the early stage of storage at 0 and $20^{\circ}C$, while increased gently at $70^{\circ}C$ with increasing storage time. After 6 days of storage, the H of Baikseolgi at $20^{\circ}C$ had a little lower than that at $0^{\circ}C$. However, the H of Baikseolgi at $70^{\circ}C$ was 10.7% of that at $0^{\circ}C$. The addition of GL had greater effect on the reduction of H than that of SE. The H of control, SE and GL additions were 336, 216 and $$174\;g_f, respectively, after 6 days at $70^{\circ}C$. The O of Baikseolgi at $70^{\circ}C$ were higher than those at $0^{\circ}C$. The O of GL added Baikseolgi had the highest value and the second and the third were SE added and control, respectively. The O of Baikseolgi decreased with increasing storage time. The C of Baikseolgi of increased with increasing storage time, which had similar curve patterns to the H of Baikseolgi. Instantaneous stress and equilibrium stress of Baikseolgi decreased with increasing storage temperature. The affection of viscous element increased and that of elastic element decreased with increasing storage temperature.

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