• Journal of Nutrition and Health
• /
• 제47권6호
• /
• pp.435-442
• /
• 2014

본 연구에서는 국가적 수준에서 지방산 섭취량을 추정할 수 있도록 국민건강영양조사에서 사용하는 식품목록에 수록된 5,144개의 식품에 대해 지방산 DB를 구축하고, 그 과정을 단계별로 정리하여 향후 개정에 활용할 수 있도록 하였다. 지방산 DB는 국내 국립기관에서 발행한 식품의 지방산 함량 분석 자료를 기본으로 하여, 국외의 권위 있는 기관에서 발간한 자료와 학술지에 보고된 함량 분석 자료를 수집한 후 자료의 질을 검토하고 대체하거나 계산하는 방법으로 구축되었다. 구축된 DB를 2011년 국민건강영양조사 자료에 적용하여 지방산 섭취량을 추정한 결과, 총 지방산 섭취량은 지방섭취량의 84% 수준으로 학술지에 보고되고 있는 섭취량 추정수준보다는 높은 수준이나 DB 구축과정에서 확인된 문제점들을 보완하여 자료의 질을 향상시키는 것이 필요할 것으로 사료된다. 본 연구는 관련 연구 분야와 국가 영양정책의 중요한 기본 자료를 확보하였다는데 의의가 있다.

• Journal of Nutrition and Health
• /
• 제30권5호
• /
• pp.529-539
• /
• 1997

The present study was to formulate a computer program to provide a menu based on a food preference survey of families in urban areas. A food preference survey was conducted by individual direct interviews of infants, children and old people in this study. In case other's food preferences were available, food menus considering all age groups could be made. Database III PLUS package was utilized for this study. The dBASE was loaded with a food compostion table of 339 different food items, along with data for amino acids and fatty acids for 171 and 43 food items respectively. Food preference, unit of food, and recommended dietary allowances were also incloded. This meun program system calculates nutrients in selected foods, and menu assessment was composed of several factors inculding energy ratio of carbohydrate : protein : fat, amino acid score, limiting amino acid, polyunsaturated fatty acid/saturated fatty acid(JP/S) ratio and Ca/P ratio.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
• /
• pp.315.3-316
• /
• 2002

A universal set of genes encodes the components of dissociated. type II. fa11y acid synthase system that is responsible for producing the multitude of fa11y acid structures found in bacterial membranes. We examined the biochemical basis for the production of fatty acids by bacteria. Several genes from HaemophHus influenzae Rd and three genes from Enterococcus faecalis V583 were predicted to encode homologs of the ${\beta}$-ketoacyl-acyl carrier protein synthases I or II or III of Escherichia coli(FabB or BabF, or FabH)were identified in the genomic database. The protein products were expressed. purified, and biochemically characterized. efFabH and hF abH carried out the initial condensation reaction of fatty acid biosynthesis with acetyl-Coenzyme A as a primer. and hFabB and efFabF1 carried out the elongation condensation reaction of fatty acid biosynthesis with myrixtoyl-ACP.

• 한국식품영양과학회지
• /
• 제28권6호
• /
• pp.1191-1200
• /
• 1999

This study was designed to determine total lipid and trans fatty acids(tFAs) content of 157 food items commonly consumed in the Korean diet and to prepare the database for the estimation of tFAs intake in Koreans. Total lipid and tFAs content was determined by Bligh and Dyer method and attenuated total reflection infrared spectroscopy, respectively. The content of tFAs in margarines ranged from 0.8% to 25.2%, depending on the manufacturers. In bakery products, cakes contained higher levels of tFAs(0.8~16.9%) than hamburgers(0.8~8.4%) and doughnuts(4.9~10%). The distribution of tFAs in crackers and cookies was wide(0.8~25%), whereas meat and fish products contained 0% to 8.9% of tFAs. Fried chickens had 0% to 14.6% of tFAs and French fries had 5.2% to 18.8% of tFAs. In noodles, nuts, chocolates and fermented fish sauces, tfAs were not detected.

• 한국식품영양학회지
• /
• 제27권3호
• /
• pp.339-347
• /
• 2014

This study investigated the classification of olive oils that are mainly distributed in Korea via imports. The fatty acid contents, degree of color, pigments, anti-oxidants, and sterol contents are analyzed on the different types of olive oil as follows: 10 kinds of extra virgin olive oil, 5 kinds of pure olive oil, and 5 kinds of refined olive-pomace oil. As a result of fatty acid analysis, the majority of oleic acid ($C_{18:1}$) and palmitic acid ($C_{16:0}$), and minority of linoleic acid ($C_{18:2}$) and stearic acid ($C_{18:0}$) were detected without any significant differences between the grades of olive oils. The UV spectrum is related to the ${\Delta}K$, and it is a part of the analysis factor for the purity and degree of degradation of the oil. Extra virgin olive oil had ${\Delta}K$ of almost 0, pure olive oil had 0.07~0.12, and refined olive-pomace oil had 0.1~0.13. These differed from extra virgin oil, and the pure or pomace oil ${\Delta}K$ had a confirmed distinct difference. The color degrees of chlorophyll with a low $L^*$ value and $(-)a^*$ (green) and carotenoid with $(+)b^*$ (yellow) were confirmed to have correlation between extra virgin and other olive oils. To compare chlorophyll and carotenoid as natural pigment in olive oils, 417 nm and the ratio of the absorbance at 480 nm (417/480) was calculated at 1.62 of extra virgin, 1.85 of pure olive oil, and 3.32 of refined olive-pomace oil. Therefore, it will be possible to distinguish when the extra virgin or pure olive oil are mixed with olive-pomace oil. The total amount of tocopherol, an anti-oxidant, were 19.06 in extra virgin, 10.91 in pure olive oil, and 27.88 in refined olive-pomace oil. The high content of tocopherol in pomace oil caused recovery of solvent extraction from olive pulp. Thus, extra virgin oil and pure olive oil were distinguished by olive-pomace oil. Polyphenol compounds in extra virgin olive oil measured high only in ferulic acid with 0.543 mg/kg, caffeic acid with 0.393 mg/kg, and other vanillic acid, vanillin, and p-coumaric acid had similar amount of 0.3 mg/kg. All grade of olive oils had the highest ${\beta}$-sitosterol content. Af (Authenticity factor) value were estimated with campesterol and stigmasterol content ratio (%). Af value was 19.2 in extra virgin olive oil, 17.1 in pure olive oil, 16.9 in refined olive-pomace oil, which were distinctive from sunflower oil with 3.7, corn oil with 2.4, and soybean oil with 2.0. It can provide important indicator of olive oil adulteration with other cheap vegetable oils. The results of this study can be used as a database for the classification of olive oil grade and distinguishing between the different types of oils.

• 한국식품과학회지
• /
• 제32권5호
• /
• pp.1002-1008
• /
• 2000

한국인 트란스 지방산 섭취량을 산출하는데 필요한 데이타 베이스 구축을 위한 기초자료를 제공할 목적으로 트란스 지방산의 주요 급원 식품인 마가린 25종, 쇼트닝 21종, 식물성 식용유지 19종, 제과류 53종, 제빵류 18종, 낙농제품 19종, 동물성 유지 및 육류 9종 등 총 164종에 대하여 트란스 지방산의 함량을 GLC에 의하여 정량하였다. 또한 이들 식품의 1회 분량당 트란스 지방산의 평균 함량을 산출하였고, 한국인 1인당 1일 트란스 지방산 섭취량을 추정하였다. 지방 100g당 트란스 지방산의 함량은 마가린 $2.11{\sim}33.83%$(평균 14.66%), 쇼트닝 $1.47{\sim}44.48%$(평균 14.21%), 식물성 식용유지 $0.18{\sim}3.82%$(평균 1.54%), 제과류 $0{\sim}45.81%$(평균 10.92%), 제빵류 $0{\sim}18.32%$(평균 7.87%), 낙농제품 $0.90{\sim}4.54%$(평균 2.27%), 동물성 유지 및 육류 $0.61{\sim}6.07%$(평균 2.24%)였다. 이들 시료 중 트란스 지방산 이성질체는 대부분이 $C_{18:1}$과 $C_{18:2}$였다. 1회분량당 트란스 지방산의 평균 함량은 마가린 0.74g, 쇼트닝 0.71g, 식물성 식용유지 0.08g, 제과류 0.57g, 제빵류 0.66g, 낙농제품 0.15g, 동물성 유지 및 육류 0.13 g이었다. 한국인의 1인당 1일 총 트란스 지방산 평균 섭취량은 약 2.3g였으며, 마가린에서 0.35g, 쇼트닝에서 0.57g, 식물성 식용유지에서 0.11g, 제과류에서 0.65g, 제빵류에서, 0.07g, 낙농제품에서 0.14g, 동물성 유지 및 육류에서 0.21g을 각각 섭취하는 것으로 추정되었다.왔다.시판 프로타민은 $470\;{\mu}g/mL$였다.는 않았다.정성을 나타내었으며, pH 9.0 및 $25^{\circ}C$의 분산매내에서 7일간 저장후에도 99% 이상의 미세캡슐이 안정한 상태로 존재함을 알 수 있었다.값을 나타내었으며, 24시간 경과 후 겔을 형성하여 겔화제로서의 이용 가능성을 보여주었다. 항산화 효과는 뚜렷한 상관관계가 발견되지 않았다.CaSO_4$, GDL, $MgCl_2$, $Ca-gluconate+MgCl_2$, $GDL+CaSO_4$ 첨가구는 0.3%에서, 그리고 $Ca-gluconate+CaSO_4$ 첨가구는 0.4%에서 높게 나타났으며 그 중 0.3% $GDL+CaSO_4$ 첨가구가 적당한 경도를 가지면서도 부드럽고 고소한 맛이 강하여 가장 높은 기호도를 나타내었다.cids은 $190^{\circ}C$까지는 $2.51{\sim}4.41$ ppm으로 거의 변화를 보이지 않다가 $210^{\circ}C$에서 18.92 ppm으로 급증하였고, 이후 $220^{\circ}C$에서 7.20ppm, $230^{\circ}C$에서 5.56 ppm으로 점진적으로 감소하였다. 이는 nonanoic acid, palmitic acid, stearic acid등이 고온가열에 의해 생성되었다가 이들이 열분해로 소실되었기 때문으로 생각된다.평균치는 34점이었으며 여윔에서는 너무 살찜으로 갈수록 사회적 체형불안도가 상승하는 경향을 나타내었다. 후쿠오카 지역의 체형 불안 도가 대구지역보다 유의적으로 높았다. 한 일 여대생의 자기 체형인식 및 체형 불안도에 관한 비교연구에서 한국여대생이 일본여대생보다 평균 신장은 크고 평균체중은 적으며 이상적으로 생각하는 신장은 한국이

• 한국식품영양학회지
• /
• 제32권6호
• /
• pp.589-597
• /
• 2019

In this study, 70 kinds of nutritional composition for five bokkeumbap products(spicy chicken, shrimp(saeu), haemul, nakji, and barbecue bokkeumbap) in high-frequency processed foods were analyzed. Raw data of intake amounts were collected from the 7th Korea National Health and Nutrition Examination Survey. Regarding proximate composition, spicy chicken bokkeumbap had lower water content and higher carbohydrate content than other bokkeumbaps, resulting in the highest total calories. The proximate composition of other bokkeumbaps were all distributed within a similar range. Vitamin content differed according to ingredients added to the bokkeumbaps. Haemul bokkeumbap had high content of vitamins B₁ and B₂, nakji bokkeumbap had high content of vitamin C and β-carotene, and barbecue bokkeumbap had high content of total niacin, folic acid, and vitamin A. For mineral content, barbecue bokkeumbap showed the highest content distribution, while nakji bokkeumbap showed the lowest content distribution. Regarding fatty acid and fat content, the highest content of fatty acids, saturated fats, and trans fats was detected in shrimp(saeu) bokkeumbap. For amino acid content, barbecue bokkeumbap showed highest content distribution. These results can be used as salient basic data for a food nutrient composition database and to significantly contribute to consumers' health.

• Asian-Australasian Journal of Animal Sciences
• /
• 제22권6호
• /
• pp.871-884
• /
• 2009

Gene expression profiling is a useful tool for identifying critical genes and pathways in metabolism. The objective of this study was to determine the major differences in the expression of genes associated with metabolism and metabolic regulation in liver and mammary tissues of lactating cows. We used the Michigan State University bovine metabolism (BMET) microarray; previously, we have designed a bovine metabolism-focused microarray containing known genes of metabolic interest using publicly available genomic internet database resources. This is a high-density array of 70mer oligonucleotides representing 2,349 bovine genes. The expression of 922 genes was different at p<0.05, and 398 genes (17%) were differentially expressed by two-fold or more with 222 higher in liver and 176 higher in mammary tissue. Gene ontology categories with a high percentage of genes more highly expressed in liver than mammary tissues included carbohydrate metabolism (glycolysis, glucoenogenesis, propanoate metabolism, butanoate metabolism, electron carrier and donor activity), lipid metabolism (fatty acid oxidation, chylomicron/lipid transport, bile acid metabolism, cholesterol metabolism, steroid metabolism, ketone body formation), and amino acid/nitrogen metabolism (amino acid biosynthetic process, amino acid catabolic process, urea cycle, and glutathione metabolic process). Categories with more genes highly expressed in mammary than liver tissue included amino acid and sugar transporters and MAPK, Wnt, and JAK-STAT signaling pathways. Real-time PCR analysis showed consistent results with those of microarray analysis for all 12 genes tested. In conclusion, microarray analyses clearly identified differential gene expression profiles between hepatic and mammary tissues that are consistent with the differences in metabolism of these two tissues. This study enables understanding of the molecular basis of metabolic adaptation of the liver and mammary gland during lactation in bovine species.

• 한국식품영양과학회지
• /
• 제46권4호
• /
• pp.529-536
• /
• 2017

본 연구에서는 산림자원에서 추출한 유지자원의 이용에 대한 잠재력을 확인하고자 지용성 영양성분에 대한 연구와 이를 토대로 한 이화학적 특성을 확인해 보았다. 영양성분의 경우 높은 불포화 지방의 비율(>80%)을 보였고, 지용성 비타민인 tocopherols과 식물성 스테롤인 phytosterol의 다양한 함량 분포를 확인하였다. 이화학적인 특성을 볼 때 1차 산화생성물인 산가와 과산화물가의 값은 안정적인 값이었으며, 2차 산화생성물의 지표인 p-anisidine value의 값을 확인하였다. 갈변도와 색도의 경우 유지 자체의 색도를 포함한 값으로 인해 신선도를 판정하기에는 무리가 있으나 색도에 대한 정보를 통해 적용 분야를 적용할 수 있는 근거로써 이용이 가능할 것으로 보인다. 산화안정성의 지표인 산소유도기간에서도 불포화지방산과 지용성 항산화제인 ${\alpha}$-T의 함량에 따른 다양한 결과를 보였다. 본 연구는 영양성분 및 이화학적인 결과를 산림유지 자원의 이용을 위한 기초자료로서 활용이 가능다고 판단된다.

• Journal of Animal Science and Technology
• /
• 제58권4호
• /
• pp.14.1-14.9
• /
• 2016

Background: Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs) in a local crossbred population (Angus - Hereford - Limousin) and evaluate their effects on meat quality traits (backfat thickness, intramuscular fat content and fatty acid composition), supporting the association tests with bioinformatic predictive studies. Results: Globally, nine SNPs were detected in the PPARG and CEBPA genes within our mixed panel, including a novel SNP in the latter. Three of these nine, along with seven other SNPs selected from the Single Nucleotide Polymorphism database (SNPdb), including SNPs in the RXRA gene, were validated in the crossbred population (N = 260). After validation, five of these SNPs were evaluated for genotype effects on fatty acid content and composition. Significant effects were observed on backfat thickness and different fatty acid contents (P < 0.05). Some of these SNPs caused slight differences in mRNA structure stability and/or putative binding sites for proteins. Conclusions: PPARG and CEBPA showed low to moderate variability in our sample panel. Variations in these genes, along with RXRA, may explain part of the genetic variation in fat content and composition. Our results may contribute to knowledge about genetic variation in meat quality traits in cattle and should be evaluated in larger independent populations.