• Title/Summary/Keyword: fatty acid analysis

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Analysis of Dietary Fiber, Mineral Content and Fatty Acid Composition in Cheonggak (Codium fragile) (청각의 식이섬유, 미네랄 함량 및 지방산 조성 분석)

  • Seo, Uk-Hyeon;Kang, Hyo-Jeong;Yoon, Ki-Bok;An, Yang-Joon;Kim, Jung-Beom
    • The Korean Journal of Food And Nutrition
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    • v.32 no.4
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    • pp.328-334
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    • 2019
  • The objective of this study was to analyze proximate composition, dietary fiber, mineral content, fatty acid composition in Cheonggak (Codium fragile) in order to encourage the consumption of Cheonggak. The proximate composition of Cheonggak was found to be 5.51% moisture, 24.09% crude ash, 15.79% crude protein, 2.47% crude lipid and 45.31% dietary fiber. The major mineral content of Cheonggak was Na 8,950 mg/100 g, Mg 1,252 mg/100 g, Ca 807 mg/100 g, K 457 mg/100 g, and trace mineral content was Fe 26 mg/100 g, Mn 8 mg/100 g, Zn 0.4 mg/100 g. Palmitic acid 36.86% and ${\alpha}$-linolenic acid 20.14% were the most contained fatty acids in Cheonggak. The ratio of saturated fatty acids to unsaturated fatty acids was 85.49% while ${\omega}-6$ fatty acids to ${\omega}-3$ fatty acids were 59.10%. Based on the proximate composition, dietary fiber, mineral content, and fatty acid composition, Cheonggak was judged to be a major source of dietary fiber and Mg and Ca, and it was deemed to be helpful in promoting health, including the prevention of hyperlipidemia. Cheonggak contained valuable nutrients similar to seaweed, which is produced and consumed the most in Korea. Thus, it is necessary to include Cheonggak in the food items.

Comparative studies on the fatty acids in the green and roasted coffee beans (커피의 원두와 배전두의 지방산조성에 관한 비교연구)

  • 고영수
    • Journal of the Korean Home Economics Association
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    • v.24 no.3
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    • pp.119-127
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    • 1986
  • To investigate differences in fatty acid composition in green coffee beans and roasted coffee beans, the extracts of two beans were subjected to analysis of fatty acid composition by GC and HPLC. The results showed that palmitic and liolneic acid were the main fatty acids of coffee oil and their contents were about 40%. Minor components, such as capric acid, lauric acid and myristic acid were detected in both samples by GC, but nut HPLC.

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A Study on the Composition of Fatty Acids of Hempseed (대마(Carnnabis sativa L.)씨의 지방산 조성에 관한 연구)

  • 고대희
    • The Korean Journal of Food And Nutrition
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    • v.3 no.2
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    • pp.201-206
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    • 1990
  • This investigation on the composition of fatty acids of hempseed through gas -chromatography analysis found the follwing results. Myristic acid and other ten materials were detected. And there was mainly composed of myristic acid 29.4%, Palmitoleic acid 16.2%, linoleic acid 14.9%, oleic acid 12.4%. It also showed that heptadecanoic acid 10.8%, erucic acid 0.5%, docosahexaenoic acid 0.3% and essential fatty acid were contained 11.9% between them. As stearic acid, docosahexaenoic acid, linoleic acid and arachidonic acid made lower cholesterol level in body, they will help prevention of senile disease with the oil d hemp seed.

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Chemical Synthesis and Antibacterial Activity of Glycerine Fatty Acid Esters Using Medium-Chain Fatty Acid (중쇄지방산을 활용한 글리세린 지방산 에스테르의 화학 합성 및 항균 활성)

  • Kyung-Haeng Lee;Eun-Hyun Lee
    • The Korean Journal of Food And Nutrition
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    • v.36 no.5
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    • pp.354-359
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    • 2023
  • A mono-type glycerine fatty acid ester compound was chemically synthesized using medium-chain fatty acids with antibacterial activity, and the physicochemical quality and antibacterial activity of the synthesized glycerine medium-chain fatty acid ester compound were measured. At a reaction molar ratio of MCT(medium chain triglyceride) to glycerine of 1:2.5, 48.15% mono ester was produced. The physicochemical analysis of the synthesized glycerine fatty acid ester compound showed an acid content of 0.38~0.60%, which tended to increase slightly as the glycerine molar ratio increased. The saponification value decreased as the synthesized molar ratio of glycerine increased from 218 to 284. The number of bacteria was measured to confirm the degree of antibacterial activity of glycerine medium-chain fatty acid esters against food poisoning bacteria, Bacillus cereus and Salmonella typhi. The number of bacteria significantly decreased as the MCT:glycerine molar ratio increased. In particular, the antibacterial effect between the treatment groups was the highest when at an MCT:glycerine molar ratio of 1:2.5.

Evaluation of Fatty Acid Composition in Korean Native Chicken Breast Meat

  • Jean Pierre Munyaneza;Eunjin Cho;Minjun Kim;Aera Jang;Hyo Jun Choo;Jun Heon Lee
    • Korean Journal of Poultry Science
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    • v.51 no.1
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    • pp.21-26
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    • 2024
  • This study was conducted to investigate the composition of the fatty acids in the breast meat of Red-brown Korean native chickens (KNC-R). This study used a total sample of three hundred eighty-two KNC-R (males: 190, females: 192). We used the fatty acid methyl ester (FAME) method to extract the fatty acids. A 2-way ANOVA of the R program was used to assess the effects of batch and sex on each fatty acid trait. Analysis of the fatty acid in the sampled population showed that the predominant fatty acid was oleic acid (C18:1; 28.252%) which is monounsaturated fatty acid (MUFA), followed by palmitic acid (C16:0; 20.895%), saturated fatty acid (SFA), and two omega-6 polyunsaturated fatty acid (PUFAs): linoleic (C18:2; 15.975%), and arachidonic (C20:4; 10.541%). Indices used to evaluate the nutritional quality of fat in the diet: ratio between PUFAs and SFAs (P/S), thrombogenicity index (TI), and atherogenicity index (AI) were calculated and were 0.959, 0.814, and 0.355, respectively. Currently, meat consumers need healthier fatty acids. Therefore, information on the content of fatty acid in chicken meat is very important for meat consumers in choosing the type of the meat to be consumed.

Effects of Procedures for Preparing Fatty Acid Methyl Esters on Fatty Acid Composition of Korean Vegetable Oils (Sesame Seed Oils) (지방산 메틸에스테르 조제방법이 한국산 식물성 기름(참깨 기름)의 지방산 조성에 미치는 영향)

  • Yoon, Tai-Heon
    • Journal of the Korean Applied Science and Technology
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    • v.4 no.1
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    • pp.9-18
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    • 1987
  • The effects of procedures for preparation of fatty acid methyl esters for gas chromatography were investigated. A quantitative comparison of four procedures for the preparation of the fatty acid methyl esters from Korean sesame seed lipids which can be representative of fatty acid ranges of Korean vegetable oils has been made. The procedures employed were $BF_3$-methanol, HCI-methanol, sodium methoxide-methanol, and tetramethylammonium hydroxide-methanol. Twelve fatty acids ranged from 14:0 to 24:0 were identified in the lipids from Korean white and black sesame seeds. All four procedures gave similar results for the fatty acids, 16:0, 18:0, 18:1, 18:2, and 18:3 present in the range of $1{\sim}44%$ but only in the HCI-methanol procedure, the fatty acids, 16:1, 20:0, 22:0, 24:0 present in the range of $0.02{\sim}1%$ showed the lowest values. When using tetramethylammonium hydroxide-methanol procedure for determination of total fatty acid composition from white and black sesame seed lipids, unsaponifiable matters including sesamol, sesamolin and sesamin present in the seed lipids are not removed from the resulting reaction mixture. Thus the transesterification mixture is used without further treatment for injection into the gas chromatography. However, the gas chromatographic analysis of the transesterification mixture showed that the unsaponifiable matters had no effect on the fatty acid composition of the seed lipids. From the results, it appears that the $BF_3$-methanol, sodium methoxide-methanol and tetramethylammonium hydroxide-methanol procedures can be used to prepare fatty acid methyl esters from Korean vegetable oils. Among the methods, the tetramethylammonium hydroxide-methanol procedure, which give total fatty acid composition, glyceride fatty acid composition and composition of free fatty acids present, appears to be a simple, convenient and quantitative procedure and applicable to samples containing broad ranges of fatty acids.

Analysis of Fatty Acid in Rice Bran Oil by Gas Chromatography (Gas Chromatography에 의(依)한 미강유(米糠油)의 지방산분석(脂肪酸分析))

  • Chung, T.M.;Shin, J.S.
    • Applied Biological Chemistry
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    • v.9
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    • pp.29-33
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    • 1968
  • Through an experiment with gas chromatography carried out using diethylene glycol succinate(DEGS) as the packing material of the column, we have obtained the correction factor between the weight ratio and the peak dimension of the saturated fatty acid methyl esters of C10, C12, C14, C16, and C18 and unsaturated fatty acid methyl esters of oleic acid, linoloic acid, and linolenic acid, employing the detector of thermal conductivity type. Quantitative analysis of the fatty acids contained in rice Bran oil was performed with the above correction factor and the results are as follows; 1. Main components were found to be palmitic acid, oleic acid sand linolenic acid. No trases of capric acid (C10) lauric acid (C10) were found. 2. It was confirmed that there were straight line relation between the logarism retention time of each fatty acid and the number of carbon of saturated fatty acid or the number of double bond of other fatty acids having the same number of carbon. 3. The correction factor became larger as to the number of carbon increased up to C18 in case of saturated fatty acids, end as for other fatty acids, and as for other fatty acids of the same carbon number, it became larger according as the number of double bond increased.

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Analysis of the Fatty Acid Composition of Cow's Milk Fat by Gas Liquid Chromatogrohy with Temperature Programming (Gas Liguid Chromatography에 의한 우유의 지방산 조성에 관한 연구)

  • Shin, Jong-Choul;Lee, Jung-Keun;Yoo, Young-Jin;Park, Ke-In
    • Korean Journal of Food Science and Technology
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    • v.4 no.3
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    • pp.213-223
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    • 1972
  • This paper chose the methods of methylesterification of the use of methoxide, the mixture solution of methanol-benzen-sulfuric acid in transesterification of the fat in cow's milk and modified powder milk and separated by gas liquid chromatography with F.F.A.P., D.E.G.A. as liquid phase. Quantitative analysis of the fatty acid of milk fat in cow's milk and modified powder milk was determined by gas liquid chromatography using the method of temperature programming which should be used to obtain satisfactory separation of short chain fatty acid on the chromatogram. It was found that the fatty acid composition of cow's milk and modified powder milk are all the major fatty acid of milk fat obtained by GLC analysis. Main components was found to be from butyric acid to arachidonic acid showing Fig. 3, 4, 5 and Table 4, 5, 6, 7, 8, 9.

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Analysis of cellular fatty acid methyl esters (FAMEs) for the identification of leuconostoc strains isolated from kimchi

  • Lee, Jung-Sook;Chun, Chang-Ouk;Kim, Hong-Joong;Joo, Yun-Jung;Lee, Hun-Joo;Park, Chan-Sum;Park, Yong-Ha;Mheen, Tae-Ick
    • Journal of Microbiology
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    • v.34 no.3
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    • pp.225-228
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    • 1996
  • The cellular fatty acid methyl esters (FAMEs) analysis data obtained for clusters defined at a Euclidian distance of 17.5, in the classification of lactic acid bacteria isolated from kimchi, described by Lee et al. (4), was used for the identification of 79 Leuconostoc isolates. The test strains were isolated using a selective isolation medium specific for the genus Leuconostoc. These strains were then characterized according to their fatty acid profiles. The results show that all seventy nine test strains were identified to the known Leuconostoc clusters B, C, and D. Cluster B had the highest relative amount of the saturated fatty acid 16 : 0. The saturated fatty acid 16 : 0 and summed feature 9 were found as a major components in cluster C, which had a higher level of summed feature 9 than cluster B. Cluster D is characterized by the highest relative amount of the unsaturated fatty acid 18 : 1 w9c. It is suggested that FAMEs analysis can be successfully applied in the identification of lactic acid bacteria isolated from kimchi.

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Analysis Method Development for Bound-MCPD (3-MCPD 지방산에스테르 분석법)

  • Woo, Sung-Min;Oh, Jae-Ho;Jang, Young-Mi;Kim, Mee-Hye
    • Journal of Food Hygiene and Safety
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    • v.25 no.4
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    • pp.294-302
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    • 2010
  • Toxicity of 3-MCPD that comes from food manufacture and processing is well-known. Recent studies reported that 3-MCPD fatty acid ester which is formed by metabolic material was 10~2000 times as much as 3-MCPD in food. This study made analysis method of 3-MCPD fatty acid esters by recent research and laboratory work, and determined the content of 3-MCPD and 3-MCPD fatty acid esters in sources and meat processing products. 3-MCPD fatty acid esters were analysed by GC/MS, which were hydrolyzed from fatty acid and then transferred 3-MCPD was extracted and reacted with derivative subject. As a result of analysis method validation, LOD was 5.4ppb, LOQ was 9.0ppb.