• 제목/요약/키워드: fatty acid analysis

검색결과 1,202건 처리시간 0.027초

Effects of Polyunsaturated Fatty Acids on Intestinal Cell Proliferation

  • Wang, Soo-Gyoung
    • Preventive Nutrition and Food Science
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    • 제4권3호
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    • pp.203-208
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    • 1999
  • The effect of the polyunsaterated fatty acids, linoleic acid(LA), arachidonic acid(AA) and conjugated dienoic linoleic acid(CLA) on IEC-6 cells (rat intestinal cell)proliferation and cell transduction have been determined in vitro. IEC-6 cells proliferation was assessed by cell growth and [3H]-thymidine incroporation analysis. At 10 μM concentration , the proliferationof cells supplemented with AA or LA was significantly higher than that of CLA. [3H]-thymidine uptake showed the same results. LA and AA increased [3H]-thymidine uptake more than CLA. The stimulatory effect of LA or AA was even more pronounced in the presence of IGF. Both cell number analysis and [3H]-thymidine incorporation revealed that IEC-6 cell proliferation was influenced differently by exogenous free fatty acids, in which AA or LA stimulated IEC-6 cell proliferation and CLA inhibited it. Tyorosine phosphorylation provides a key switch to regulate celluar acitivity in response to extracellular stimuli. At 20 μM and 10μM, AA with IGF-1 stimulated protein tyrosine phophorylation in IEC-6 cells, but LA's impact was less than that of AA. CLA and CLA with IGF-1 inhibited protein tyrosine phosphorylation in IEC-6 cells. These results suggest there is a possible correlation between cell proliferation and IGF receptor tyrosine knase activity driven by AA.

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황색종 잎담배의 건조과정중 비휘발성 유기산과 지방산의 변화에 관한 연구 (Studies on the Change of Nonvolatile Organic acids and Higher Fatty acids during the curing of Flue-cured tobaccos)

  • 이문수;이운철
    • 한국연초학회지
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    • 제4권2호
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    • pp.75-80
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    • 1982
  • During the process of curing of the tobacco (Nicotiana tabacum L. ) leaves the concentration change of Nonvolatile organic acids and Higher fatty acids of the flue-cured tobacco leaves was observed. The contents of Nonvolatile organic acids in flue-cured tobacco leaves were as follows, malic> oxalic>malonic>citric>succinic acid. Higher fatty acids was linolenic>Palmitic>oleic >stearic$\geqq$linoleic acid, Citric acid content of last curing time was increased about 4~5 times in compare to before curing tobacco. It was concluded that significant changes of three variety flue-cured tobacco but a significant difference was observed in the composition of Daegu gold variety.

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나전칠기함(螺鈿漆器函) 모서리 보강재료(補强材料)의 잔존지방산(殘存脂肪酸) 분석(分析) (Analysis of Residual Fatty acid of the material for Reinforcement used on Edges of Lacquer ware inlaid box with Mother-of-pearl in Chŏson Dynasty)

  • 유혜선
    • 박물관보존과학
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    • 제1권
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    • pp.53-59
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    • 1999
  • 조선시대 나전칠기함(유물번호 : 덕4182) 상태조사 중에 발견한 백골(白骨)의 모서리 보강재(補强材)에 대한 잔존지방산(殘存脂肪酸) 분석(分析)을 실시하였다 그 결과 콜레스테롤의 함량이 높아 동물성 재료임을 확인할 수 있었고, 가스크로마토그라프 분석을 통해 그 지방산 조성(組成)이 상어가죽[교피(鮫皮)]과 유사한 패턴을 보임을 알 수 있었다. 이 분석결과에 따라 나전칠기함의 손상된 보강재를 새로운 상어가죽으로 대체(代替) 복원(復元)하였다.

Differences in Health-related Fatty Acids, Intramuscular Fat and the Physico-chemical Quality in Mutton as Affected by Season, Place of Purchase and Meat Portion

  • Rani, Zikhona T.;Nantapo, Carlos W.T.;Hugo, Arnold;Muchenje, Voster
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권11호
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    • pp.1630-1637
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    • 2014
  • The objective of the study was to determine the quality and fatty acid profiles of mutton cuts purchased from rural and urban localities in South Africa. Five hundred and ten samples were collected in four seasons from both rural and urban shops and butcheries. Samples were immediately transported to the laboratory in cooler boxes with ice where the following physico-chemical characteristics of mutton were determined; meat pH, color ($L^*$, $a^*$, and $b^*$), cooking losses and Warner Braztler shear force and replicates stored at $-20^{\circ}C$ pending fatty acid analysis. Meat $L^*$ values were lowest ($24.7{\pm}0.49$) in winter and highest ($32.2{\pm}0.49$) in spring. The loin and sirloin cuts recorded the highest intramuscular fat whilst rib and leg cuts recorded the lowest intramuscular fat. In conclusion intramuscular fat, fatty acid profiles and physico-chemical quality of mutton were significantly affected by season and meat portion and not necessarily by the locality and class of shop.

고콜레스테롤혈증 여성 환자의 지방산 섭취량 비교 (Comparisons of Fatty Acid intake of Hypercholesterolemia in Women)

  • 이승림;김상연;장유경
    • 대한영양사협회학술지
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    • 제7권4호
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    • pp.373-384
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    • 2001
  • The purpose of this study was to compare with in fatty acid intakes between hypercholesterolemia group and normocholesterolemia group, aged between 30 to 65 years old in women. The subjects were classified as hypercholesterolemia group and normocholesterolemia group based on The Guideline for Korean Hyperlipidemia. Dietary intakes of fatty acids were by means of a 24-hr recall method with food models and measuring tools. We analyzed both data sets together using analysis of variance the chi-square test and student's t-test(SPSS for WINDOWS, version 7.5). Significance was defied as a P value <0.05. The results obtained are summarized as follows. Mean age, BMI and percentage of postmenopausal women in hypercholesterolemia group were significantly higher than those of normocholesterolemia group. Percent of total energy from fat in hypercholesterolemia group tended to be higher than that in normocholesterolemia group. C16:0(Palmitic acid) and saturated fatty acids(SFA) intake of hypercholesterolemia group was significantly higher than that of normocholesterolemia group. Therefore, women who suffered from hypercholesterolemia were recommended to control body weight and consume foods containing low SFA and cholesterol.

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오염중의 유이지방산이 세척에 미치는 영향(제일보 팔미트산의 세척 특성) (Studies on the Detergency Characteristics of Free Fatty Acid in Oily Soil. Part 1. Detergency of Palmitic Acid.)

  • 정혜원;김성련
    • 한국의류학회지
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    • 제1권1호
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    • pp.31-37
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    • 1977
  • The effects of surfactants and concentration of NaOH in surfactant solution on the removal of free fatty acid soil from cotton fabrics were investigated. Cotton fabrics were soiled with palm itic acid which is the most common fatty acid found in natural oily soil and washed in Lauder-ometer with various types of surfactant with or without NaOH. The rate of soil removal was estimated by analyzing palmitic acid contents in fabric before ar d after washing. Analysis of palmitic contents was made by extracting palmitic acid with azeotropic mixture of alcohol-benzene and the extracts were titrated with standard NaOH solution. It was shown that the types of surfactant are important factor in free fatty acid removal and the efficiency increases in the following order: SLS$90\%$ of initial sorption. In relation to the mechanism of detergency, the suspending and emulsifying power of surfactants were also examined. From the results of this experiments, it could be concluded that the soap formation with alkali and the suspending power of surfactant arc significant factors in free fatty acid soil removal, but the emulsifying power of it is neglizible.

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동백나무와 차나무 기름의 지방산 조성 및 메틸에스테르 특성 분석 (Analysis of Fatty Acid Composition and Methyl-ester Properties of Camellia and Tea Oil)

  • 김광수;이영화;장영석;최인후
    • 신재생에너지
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    • 제9권3호
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    • pp.36-42
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    • 2013
  • To secure raw materials of biodiesel production, the possibility of camellia (C. japonica L.) and tea (C. sinensis L.) seed oil was studied to produce biodiesel. In this research, crude oil contents and fatty acid compositions of seeds were analyzed by Solxlet and Gas chromatography (GC). The oil contents in the seeds of camellia were 69.8%~73.8%, and tea were 26.3%~29.4%. Among the fatty acids of camellia and tea oil, oleic acid was dominant. The unsaturated fatty acids accounted for 88.4% and 80.2% of the whole fatty acids of camellia and tea seed oil. Total seed oil content and fatty acid composition of tea seed were influenced by collecting date. Across maturation period, oil content of tea seed averaged 18.3% on $6^{th}$ September increasing to 27.9% by $11^{th}$ October. For largest seed yield and oil content, the optimum time to harvest tea is in middle october, and camellia is late september and thereafter. The extraction efficiency of oil from seeds by extraction methods was determined. Biodiesel were synthesized in 92.1~92.8% yields from camellia and tea oils by transesterification. The biodiesel was characterized by its physical and fuel properties including oxidation stability, iodine value and cold filter plugging point (CFPP). Oxidation stability of camellia was 8.6~8.8 hours and tea was 2.9~3.6 at $110^{\circ}C$. Camellia oil had considerably better oxidation stability and CFPP than tea oil.

Muscle Fiber Characteristics and Fatty Acid Compositions of the Four Major Muscles in Korean Native Black Goat

  • Hwang, Young-Hwa;Joo, Sung-Hyun;Bakhsh, Allah;Ismail, Ishamri;Joo, Seon-Tea
    • 한국축산식품학회지
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    • 제37권6호
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    • pp.948-954
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    • 2017
  • The objective of this study was to investigate the relationship between muscle fiber characteristics and fatty acid composition of four major muscles in Korean native black goat (KNBG). Longissimus lumborum (LL), psoas major (PM), semimembranosus (SM), and gluteus medius (GM) were obtained from five male KNBGs of 36 mon of age and subjected to histochemical analysis and to determine fatty acid composition and meat quality traits. There were significant (p<0.05) differences in fiber number percentage (FNP) and fiber area percentage (FAP) of fiber types among these four muscles. PM had the highest FNP of type I and the lowest FNP of type IIB, while SM had the highest FNP of type IIB. The highest fat content was observed in LL while SM had the lowest fat content. The proportions of SFA and MUFA were significantly (p<0.05) different among four muscles due to differences in the majority of fatty acids such as oleic (C18:1) and palmitic (C16:0) acids. The PUFA/SFA ratio was significantly (p<0.05) different among four muscles, and the highest PUFA/SFA ratio was observed in PM. Results suggested that LL and PM might be healthful because of higher desirable fatty acid value and PUFA/SFA ratio, respectively. Also, data showed that correlations between muscle fiber types and fatty acids proportion of goat muscles were reversed with those of cattle muscles.

배양 조건을 달리한 Dunaliela tertiolecta의 조체내 지방산 분석 (Evaluation of Fatty Acids in Dunaliela tertiolecta, in Various Culture Conditions)

  • 윤덕현;전중균;박철원
    • 한국양식학회지
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    • 제2권1호
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    • pp.43-51
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    • 1989
  • 녹조 편모류인 Dunaliella tertiolecta Butter를 광도, 광주기 및 온도 조건을 달리하여 배양한 후 조체내의 지방산 조성을 분석하였다. 광주기와 온도 조건은 D. tertiolecta의 성장에 뚜렷한 영향을 주었고, 성장기에 최대 세포수는 $2.32{\times}10^6$, 세포 분열율은 1.97 division/day였다. 여러조건에서 배양한 조체의 지방산을 분석한 결과Cl6(Palmitic acid), C18 : 3$\omega$3 (cis-Linolenic acid)가 주성분이었으며 EPA나 DHA와 같은 고도의 불포화 지방산은 소량 검출되었다. 불포화 지방산(Polyene)은 배양 온도가 낮을수록 증가되었으며, 광도는 불포화 지방산 함량에 별로 영향을 미치지 못했다. 광주기 조건에서, 명주기를 오래할수록 D. tertiolecta의 성장은 증가했지만, 반면에 조체내의 불포화 지방산(Polyene)은 감소되는 것으로 나타났다.

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Biotransformation of Intestinal Bacterial Metabolites of Ginseng Saponin to Biologically Active Fatty-acid Conjugates

  • Hasegawa Hideo;Saiki Ikuo
    • 고려인삼학회:학술대회논문집
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    • 고려인삼학회 2002년도 학술대회지
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    • pp.317-334
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    • 2002
  • Ginsenosides are metabolized (deglycosylated) by intestinal bacteria to active forms after oral administration. 20(S)-Protopanaxadiol $20-O-{\beta}-D-glucopyranoside$ (M1) and 20(S)-protopanaxatriol (M4) are the main intestinal bacterial metabolites (IBMs) of protopanaxadiol- and protopanaxatriol-type glycosides. M1 was selectively accumulated into the liver soon after its intravenous (i.v.) administration to mice, and mostly excreted as bile; however, some M1 was transformed to fatty acid ester (EMl) in the liver. EM1 was isolated from rats in a recovery dose of approximately $24mol\%.$ Structural analysis indicated that EM1 comprised a family of fatty acid mono-esters of M1. Because EM1 was not excreted as bile as Ml was, it was accumulated in the liver longer than M1. The in vitro cytotoxicity of M1 was attenuated by fatty acid esterification, implying that esterification is a detoxification reaction. However, esterified M1 (EM1) inhibited the growth of B16 melanoma more than Ml in vivo. The in vivo antitumor activity paralleled with the pharmacokinetic behavior. In the case of M4, orally administered M4 was absorbed from the small intestine into the mesenteric lymphatics followed by the rapid esterification of M4 with fatty acids and its spreading to other organs in the body and excretion as bile. The administration of M4 prior to tumor injection abrogated the enhanced lung metastasis in the mice pretreated with 2-chloroadenosine more effectively than in those pretreated with anti-asialo GMl. Both EM1 and EM4 did not directly affect tumor growth in vitro, whereas EM1 promoted tumor cell lysis by lymphocytes, particularly non-adherent splenocytes, and EM4 stimulated splenic NK cells to become cytotoxic to tumor cells. Thus, the esterification of IBM with fatty acids potentiated the antitumor activity of parental IBM through delay of the clearance and through immunostimulation. These results suggest that the fatty acid conjugates of IBMs may be the real active principles of ginsenosides in the body.

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