• 생명과학회지
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• 제31권10호
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• pp.913-921
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• 2021

고관절과 어깨의 점진적인 근육 약화를 특징으로 하는 지대형 근이영양증(limb-girdle muscular dystrophy: LGMD)은 우성 및 열성 유전을 모두 보여주며, TTN을 비롯한 많은 유전자가 발병과 관련된 것으로 알려져 있다. 본 연구는 40대 중반의 늦은 발병을 나타낸 상염색체 열성 LGMD 및 심방 조동의 증상을 가진 한 남성 환자의 유전적 원인을 규명하기 위해 수행되었다. 전장 엑솜 서열분석을 수행하여 환자로부터 TTN 유전자의 복합 이형 접합성 변이의 대립유전자를 동정하였다. 한 대립유전자는 [c.24124G>T (p.V8042F)]의 단일 변이를 보였지만, 다른 대립유전자는 [c.29222G>C (p.R9741P) + c.67490A>G (p.H22497R) + c.75376C>T (p.R25126C)]의 세 변이로 구성된 단상형이었다. 대립유전자 중 p.V8042F는 어머니로부터 유전된 반면, 다른 단상형 대립유전자는 아버지로부터 유전된 것으로 추정되었다. 본 연구에서 분리된 TTN 변이들은 공공 인간 유전체 데이터베이스(1,000 Genomes, gnomAD 및 KRGDB)에서 보고되지 않았거나 매우 낮은 빈도로 보고되었다. 대부분의 변이들은 고도로 보존된 면역글로불린 또는 피브로넥틴 도메인에 위치했으며, 일부 in silico 분석에 의해 병원성인 것으로 예측되었다. TTN 거대 단백질은 근육 조립, Z-라인에서 힘 전달, I-밴드에서 안정 장력 유지에 중요한 역할을 한다. 결론적으로, 우리는 이러한 이형접합성 복합 돌연변이의 이중 대립유전자가 LGMD 표현형의 유전적 원인으로서 작용할 수 있을 것으로 제시한다.

• 한국식품영양과학회지
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• 제40권2호
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• pp.183-195
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• 2011

서울 시내와 경기도 파주시에 소재하는 남녀 공학 고등학교의 식생활 실태 중 식사 규칙성과 식사횟수와 관련된 사항에 대하여 조사하였다. 남녀 고등학생의 식사의 규칙성에서는 남학생에 비해 여학생의 경우 상대적으로 하루 식사가 비교적 규칙적인 것으로 나타났다. 1일 동안의 식사횟수는 남녀학생 모두 대체로 '1일 3식'을 하는 것으로 나타났다. 식사섭취횟수의 형성 시기에 대한 결과에서는 남녀학생은 모두 '중학교'로 나타났으며, 특히 여학생에 있어서는 '학령기 이전'이라고 답한 경우도 28.7%로 높게 나타났다. 활동에 가장 영향을 주는 식사에 대한 답변은 남학생은 점심(41.0%)이라고 답한 결과가 가장 높은 반면 여학생은 '아침'(39.8%)이라는 결과가 높게 나타났다. 학년별 식사섭취 빈도와 규칙성의 항목에 대한 분석 결과에서는 전반적으로 규칙적인 식사를 하고 있는 것으로 나타났지만 유의차가 없었다(p<0.05). 1일 식사횟수에서는 1~3학년 모두 대체적으로 '1일 3식'을 하는 것으로 나타났다. 학년별 식사섭취횟수 형성시기에서는 모든 학년에서 '중학교'와 '학령기 전'의 순의 결과를 보여 주었다. 식사의 규칙성과 일반사항의 분석 결과, 가족 수 '5명'과 '용돈이 적을수록'의 항목에서 규칙적인 식사가 이루어지고 있었다. 용돈 중 '3만원 이하'의 경우 '5만원 이상'의 식비 지출의 경우 비교적 규칙적인 것으로 나타났다. 식사를 준비하는 사람에 따라서는 '어머니'가 식사를 준비하는 경우 상대적으로 하루 식사가 비교적 규칙적인 것으로 나타났다. 부모의 연령과 교육정도가 높고 월 가구 소득이 많을수록 하루 식사가 비교적 규칙적으로 나타났다. 부모의 직업에 따라서는 아버지 '사무직'(149명(56.9%))과 어머니 '전업주부'(279명(56.9%))인 경우 하루 식사가 비교적 규칙적인 것으로 나타났다. 1일 동안의 식사횟수와 일반사항과의 차이분석 결과에서는 대체적으로 '1일 3식'을 하는 것으로 볼 수 있었다. 한 달 용돈 '5만원 이하'의 경우 54.3%가 '1일 3식'을 하는 것으로 나타났고 식사를 준비하는 사람의 경우, '스스로' 이외에는 대체적으로 1일 3식을 하는 것으로 나타났다. 부모의 연령, 부모 학력, 월 가족소득에 따라 식사횟수와 차이를 보여주었다. 식사 섭취횟수의 형성 시기와 일반사항을 분석한 결과, '용돈'과 ' 식사를 준비하는 사람'의 결과는 '중학교'>'학령기전' 순으로 나타났다. 수면 시간과의 분석결과에서는 '5~8시간'이라고 응답한 학생들의 경우 '중학교 때 형성되었다'에서 높은 결과를 보였다. 또한 부모 연령이 낮을수록 '중학교 때 형성되었다'는 결과가 높았고 연령이 높아질수록 '학령기 전'의 결과가 높게 나타났다. 월가구소득과 식사섭취횟수와의 분석 결과에서는 소득이 높을수록 '학령기 전'이라고 답변한 결과가 높았고 소득이 낮을수록 '중학교 때 형성되었다'에서 높은 결과를 보여 주었다. 전체적으로 볼 때, '중학교 때 형성되었다'가 34.6%으로 가장 높게 나타났고, '학령 전'(23.8%), '초등학교 4~6학년 전'(13.7%), '초등학교 2학년 전'(11.8%) 순으로 나타났다. 한 끼 식사 중 자신활동에 가장 큰 영향을 미치는 식사를 분석한 결과에서는 '점심'이 37.6%으로 가장 높게 나타났고, '아침'(35.8%), '저녁'(22.8%) 순으로 나타나 전반적 '점심'이 자신의 활동에 가장 큰 영향을 미친다고 생각하는 것을 볼 수 있었다. 한 달 용돈 '9만원 이하'의 경우 '점심'이, '10~20만원'의 경우 '아침'이, '21만원 이상'의 경우 '저녁'이 가장 큰 영향을 미친다고 하여 차이를 보였다. 수면시간과의 분석 결과에서는 4, 5, 6시간의 경우 '아침'이, '7시간 이상' 수면을 하는 경우 '점심'이 가장 큰 영향을 미친다는 결과였다. 아버지의 연령은 낮을수록 어머니의 연령은 높을수록 '아침'의 영향력이 크다는 상반된 결과가 나타났다. 또한 월가구소득이 증가할수록 '아침'의 영향력을 높게 느끼고 있는 결과였다. 이상의 결과를 종합해 보면, 고등학생의 식생활태도는 성별과 학년별로 차이를 나타내어 차별화된 영양교육과 식생활 태도에 관련한 기초지식을 강화해야 함을 알 수 있었다. 식사의 규칙성과 식사횟수에 있어서는 부모의 특성과 월 가족 소득에서 차이가 나타나 고등학생 개인의 영양교육은 물론이고 가정을 연계한 영양지도에 대한 대책이 필요한 것을 확인할 수 있었다. 식사횟수의 형성은 이미 고등학생 이전에 만들어져 고정되어 있는 결과가 나타나기에 특히 다양한 교육방법을 활용하여 그 중요성을 인지하도록 만들고 올바른 식습관으로 개선된 후 유지되도록 지속적인 모니터링이 요구되었다. 아침식사보다 점심식사에 대한 영향력이 나타난 결과를 보면, 아침식사에 대한 결식과 영양불균형을 예측해 볼 수 있었고 영양 섭취가 세 끼니에 골고루 분배될 수 있는 영양지도의 중요성과 아침식사와 결식에 고등학생의 식생활태도의 후속연구를 진행해야 할 필요성을 확신할 수 있었다. 본 조사의 결과는 남녀 고등학생이 식생활태도와 영향을 미치는 요인들과 관련하여 분석해서, 실천적인 식습관 형성 및 개선에 대한 개별적이면서 세부적인 영양교육 프로그램을 구축하는데 있어 기초 자료가 활용되어지길 바란다. 또한 지속적이면서 다각적인 식사관련 후속 연구들이 진행되어 영양교육 시간이 정규 수업시간으로 정착하는 데 있어 도움이 되리라 생각된다.

• 보건교육건강증진학회지
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• 제18권1호
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• pp.79-91
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• 2001

본 연구는 초등학교 6학년 아동을 대상으로 비만상태에 따른 체형만족도와 체형존중감과의 관계를 알아보기 위하여 1999년 5월 1일부터 5월31일까지 제천시 5개 초등학교 6학년 아동 669명을 대상으로 신체계측 자료와 설문지 조사를 이용하여 자료수집을 하였으며 그 결과는 다음과 같다. 1. 비만상태는 전체의 14.0%, 남자의 21.4%, 여자의 6.6%가 비만이었으며, 평균체중은 남자가 41.09kg(표준편차, 9.102), 여자가 39.99kg(표준편차, 7.743)이었고 평균신장은 남자가 147.51cm(표준편차, 7.122), 여자가 149.13cm (표준편차, 6.852)이었다. 2. 비만상태에 따른 체형만족도는 전체($chi^2$=110.31, p= .000), 남($chi^2$=99.97, p= .000), 여 ($chi^2$=62.23, p= .000) 모두 유의한 차이가 있었다. 전체에서는 저체중군의 38.8%, 정상체중군의 32.0%, 과 체중군의 24.29%, 비만군의 10.6%가 체형에 대해 만족하였고, 남자에서는 저체중군의 25.7%, 정상체중군의 28.8%, 과체중군의 31.7%, 비만군의 12.5%가 체형에 대해 만족하였으며, 여자에서는 저체중군의 42.3%, 정상체중군의 23.7%, 과체중군의 12.0%, 비만군의 4.5%가 체형에 대해 만족하였다. 3. 비만상태에 따른 체형존중감은 전체(F=8.634, p= .000), 남(F=11.738, p= .000), 여 (F=4.502, p= .004) 모두 유의한 차이가 있었다. 평균 체형존중감 점수는 전체에서 정상체중군이 12.764점(표준편차 5.344)으로 가장 높았고 비만군이 9.840점(표준편차 4.638)로 가장 낮았으며, 남자에서는 과체중군이 14.780점(표준편차 4.601), 여자에서는 저체중군이 12.284점(표준편차 4.604)으로 체형존중감이 가장 높았고, 남(평균 10.166점, 표준편차 4.246), 여(평균 8.772점, 표준편차 5.723) 모두 비만군에서 체형존중감이 가장 낮았다. 4. 비만도와 체형만족도 및 체형존중감과의 상관관계는 비만도와 체형만족도(r= -.2081, p= .000), 비만도와 체형존중감(r= -.2250, p= .000)은 유의한 역상관관계를 나타내어 비만도가 높을수록 체형만족도와 체형존중감이 낮았다. 5. 일반적 특성에 따른 비만상태는 전체에서는 성별($chi^2$=88.136, p=.000), 체형에 대한 인식($chi^2$=242.371, p= .000)에서, 남자에서는 부의 체형($chi^2$=15.11, p= .019), 체형에 대한 인식($chi^2$=138.95, p= .000)에서, 여자에서는 모의 학력($chi^2$=19.46, p= .022), 체형에 대한 인식($chi^2$=143.35, p= .000)에서 유의한 차이가 있었다. 전체에서 성별은 남자에서 비만은 21.4%, 여자에서 비만은 6.6%로 남자가 비만인 경우가 더 많았고, 체형에 대한 인식은 자신의 체형이 말랐다고 인식하는 경우 비만은 0.7%, 보통이라고 인식하는 경우 비만은 9.3%, 비만하다고 인식하는 경우 비만은 64.6%로 나타났다. 남자에서 부의 체형이 마른 경우 실제 비만인 학생은 21.20%, 보통인 경우 19.1%, 비만인 경우 42.9%로 나타났고, 자신의 체형에 대한 인식은 체형이 말랐다고 인식하는 경우 비만은 1.3%, 보통이라고 인식하는 경우 비만은 16.6%, 비만이라고 인식하는 경우 비만은 83.4% 이었다. 여자에서 모의 학력이 초등졸에서는 실제 비만은 없었고 중졸에서는 비만이 11.1%, 고졸에서는 4.9%, 대졸이상에서는 13.2%로 나타났고, 체형에 대한 인식에서는 말랐다고 인식하는 경우 실제 비만은 없었고, 보통이라고 인식한 경우 비만은 2.6%, 비만하다고 인식한 경우 비만은 43.3% 이었다.

• 대한한의학원전학회지
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• 제20권4호
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• pp.211-250
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• 2007

1. The 'Kao Zheng Pai(考證派) comes from the 'Zhe Zhong Pai' and is a school that is influenced by the confucianism of the Qing dynasty. In Japan Inoue Kinga(井上金娥), Yoshida Koton(吉田篁墩) became central members, and the rise of the methodology of historical research(考證學) influenced the members of the 'Zhe Zhong Pai', and the trend of historical research changed from confucianism to medicine, making a school of medicine based on the study of texts and proving that the classics were right. 2. Based on the function of 'Nei Qu Li '(內驅力) the 'Kao Zheng Pai', in the spirit of 'use confucianism as the base', researched letters, meanings and historical origins. Because they were influenced by the methodology of historical research(考證學) of the Qing era, they valued the evidential research of classic texts, and there was even one branch that did only historical research, the 'Rue Xue Kao Zheng Pai'(儒學考證派). Also, the 'Yi Xue Kao Zheng Pai'(醫學考證派) appeared by the influence of Yoshida Kouton and Kariya Ekisai(狩谷掖齋). 3. In the 'Kao Zheng Pai(考證派)'s theories and views the 'Yi Xue Kao Zheng Pai' did not look at medical scriptures like the "Huang Di Nei Jing"("黃帝內經") and did not do research on 'medical' related areas like acupuncture, the meridian and medicinal herbs. Since they were doctors that used medicine, they naturally were based on 'formulas'(方劑) and since their thoughts were based on the historical ideologies, they valued the "Shang Han Ja Bing Lun" which was revered as the 'ancestor of all formulas'(衆方之祖). 4. The lives of the important doctors of the 'Kao Zheng Pai' Meguro Dotaku(目黑道琢) Yamada Seichin(山田正珍), Yamada Kyoko(山田業廣), Mori Ritsi(森立之) Kitamura Naohara(喜多村直寬) are as follows. 1) Meguro Dotaku(目黑道琢 1739${\sim}$1798) was born of lowly descent but, using his intelligence and knowledge, became a professor as a Shi Jing Yi(市井醫) and as a professor for 34 years at Ji Shou Guan mastered the "Huang Di Nei Jing" after giving over 300 lectures. Since his pupil, Isawara Ken taught the Lan Men Wu Zhe(蘭門五哲) and Shibue Chusai, Mori Ritsi(森立之), Okanishi Gentei(岡西玄亭), Kiyokawa Gendoh(淸川玄道) and Yamada Kyoko(山田業廣), Meguro Dotaku is considered the founder of the 'Yi Xue Kao Zheng Pai'. 2) The family of Yamada Seichin(山田正珍 1749${\sim}$1787) had been medical officials in the Makufu(幕府) and the many books that his ancestors had left were the base of his art. Seichin learned from Shan Ben Bei Shan(山本北山), a 'Zhe Zhong Pai' scholar, and put his efforts into learning, teaching and researching the "Shang Han Lun"("傷寒論"). Living in a time between 'Gu Fang Pai'(古方派) member Nakanishi Goretada(中西惟忠) and 'Kao Zheng Pai' member Taki Motohiro(多紀元簡), he wrote 11 books, 2 of which express his thoughts and research clearly, the "Shang Han Lun Ji Cheng"("傷寒論集成") and "Shang Han Kao"("傷寒考"). His comparison of the 'six meridians'(3 yin, 3 yang) between the "Shang Han Lun" and the "Su Wen Re Lun"("素問 熱論) and his acknowledgement of the need and rationality of the concept of Yin-Yang and Deficient-Replete distinguishes him from the other 'Gu Fang Pai'. Also, his dissertation of the need for the concept doesn't use the theories of latter schools but uses the theory of the "Shang Han Lun" itself. He even researched the historical parts, such as terms like 'Shen Nong Chang Bai Cao'(神農嘗百草) and 'Cheng Qi Tang'(承氣湯) 3) The ancestor of Yamada Kyoko(山田業廣) was a court physician, and learned confucianism from Kao Zheng Pai 's Ashikawa Genan(朝川善庵) and medicine from Isawa Ranken and Taki Motokata(多紀元堅), and the secret to smallpox from Ikeda Keisui(池田京水). He later became a lecturer at the Edo Yi Xue Guan(醫學館) and was invited as the director to the Ji Zhong(濟衆) hospital. He also became the first owner of the Wen Zhi She(溫知社), whose main purpose was the revival of kampo, and launched the monthly magazine Wen Zi Yi Tan(溫知醫談). He also diagnosed and prescribed for the prince Ming Gong(明宮). His works include the "Jing Fang Bian"("經方辨"), "Shang Han Lun Si Ci"("傷寒論釋司"), "Huang Zhao Zhu Jia Zhi Yan Ji Yao"("皇朝諸家治驗集要") and "Shang Han Ja Bing Lun Lei Juan"("傷寒雜病論類纂"). of these, the "Jing Fang Bian"("經方辨") states that the Shi Gao(石膏) used in the "Shang Han Lun" had three meanings-Fa Biao(發表), Qing Re(淸熱), Zi Yin(滋陰)-which were from 'symptoms', and first deducted the effects and then told of the reason. Another book, the "Jiu Zhe Tang Du Shu Ji"("九折堂讀書記") researched and translated the difficult parts of the "Shang Han Lun", "Jin Qui Yao Lue", "Qian Jin Fang"("千金方"), and "Wai Tai Mi Yao"("外臺秘要"). He usually analyzed the 'symptoms' of diseases but the composition, measurement, processing and application of medicine were all in the spectrum of 'analystic research' and 'researching analysis'. 4) The ancestors of Mori Rits(森立之 1807${\sim}$ 1885) were warriors but he became a doctor by the will of his mother, and he learned from Shibue Chosai(澁江抽齋) and Isawaran Ken and later became a pupil of Shou Gu Yi Zhai, a historical research scholar. He then became a lecturer of medical herbs at the Yi Xue Guan, and later participated in the proofreading of "Yi Xin Fang"("醫心方") and with Chosai compiled the "Jing Ji Fang Gu Zhi"("神農本草經"). He visited the Chinese scholar Yang Shou Jing(楊守敬) in 1881 and exchanged books and ideas. Of his works, there are the collections(輯複本) of "Shen Nong Ben Cao Jing"(神農本草經) and "You Xiang Yi Hwa"("遊相醫話") and the records, notes, poems, and diaries such as "Zhi Yuan Man Lu"("枳園漫錄") and "Zhi Yuan Sui Bi"("枳園隨筆") that were not published. His thoughts were that in restoring the "Shen Nong Ben Cao Jing", "the herb to the doctor is like the "Shuo Wen Jie Zi"("說文解字") to the scholar", and he tried to restore the ancient herbal text using knowledge of medicine and investigation(考據). Also with Chosai he compiled the "Jing Ji Fang Gu Zhi"("經籍訪古志") using knowledge of ancient text. Ritzi left works on pure investigation, paid much attention to social problems, and through 12 years of poverty treated all people and animals in all branches of medicine, so he is called a 'half confucianist half doctor'(半儒半醫). 5) Kitamurana Ohira(喜多村直寬 1804${\sim}$1876) learned scriptures and ancient texts from confucian scholar Asaka Gonsai, and learned medicine from his father Huai Yaun(槐園). He became a teacher in the Yi Xue Guan in his middle ages, and to repay his country, he printed 266 volumes of "Yi Fang Lei Ju("醫方類聚") and 1000 volumes of "Tai Ping Yu Lan"("太平禦覽") and devoted it to his country to be spread. His works are about 40 volumes including "Jin Qui Yao Lue Shu Yi" and "Lao Yi Zhi Yan" but most of them are researches on the "Shang Han Za Bing Lun". In his "Shang Han Lun Shu Yi"("傷寒論疏義") he shows the concept of the six meridians through the Yin-Yang, Superficial or internal, cold or hot, deficient or replete state of diseases, but did not match the names with the six meridians of the meridian theory, and this has something in common with the research based on the confucianism of Song(宋儒). In clinical treatment he was positive toward old and new methods and also the experience of civilians, but was negative toward western medicine. 6) The ancestor of the Taki family Tanbano Yasuyori(丹波康賴 912-955) became a Yi Bo Shi(醫博士) by his medical skills and compiled the "Yi Xin Fang"("醫心方"). His first son Tanbano Shigeaki(丹波重明) inherited the Shi Yao Yuan(施藥院) and the third son Tanbano Masatada(丹波雅忠) inherited the Dian You Tou(典藥頭). Masatada's descendents succeeded him for 25 generations until the family name was changed to Jin Bao(金保) and five generations later it was changed again to Duo Ji(多紀). The research scholar Taki Motohiro was in the third generation after the last name was changed to Taki, and his family kept an important part in the line of medical officers in Japan. Taki Motohiro(多紀元簡 1755-1810) was a teacher in the Yi Xue Guan where his father was residing, and became the physician for the general Jia Qi(家齊). He had a short temper and was not good at getting on in the world, and went against the will of the king and was banished from Ao Yi Shi(奧醫師). His most famous works, the "Shang Han Lun Ji Yi" and "Jin Qui Yao Lue Ji Yi" are the work of 20 years of collecting the theories of many schools and discussing, and is one of the most famous books on the "Shang Han Lun" in Japan. "Yi Sheng" is a collection of essays on research. Also there are the "Su Wen Shi"("素問識"), "Ling Shu Shi"("靈樞識"), and the "Guan lu Fang Yao Bu"("觀聚方要補"). Taki Motohiro(多紀元簡)'s position was succeeded by his third son Yuan Yin(元胤 1789-1827), and his works include works of research such as "Nan Jing Shu Jeng"("難經疏證"), "Ti Ya"("體雅"), "Yao Ya"("藥雅"), "Ji Ya"("疾雅"), "Ming Yi Gong An"("名醫公案"), and "Yi Ji Kao"("醫籍考"). The "Yi Ji Kao" is 80 volumes in length and lists about 3000 books on medicine in China before the Qing Dao Guang(道光), and under each title are the origin, number of volumes, state of existence, and, if possible, the preface, Ba Yu(跋語) and biography of the author. The younger sibling of Yuan Yin(元胤 1789-1827), Yuan Jian(元堅 1795-1857) expounded ancient writings at the Yi Xue Guan only after he reached middle age, was chosen for the Ao Yi Shi(奧醫師) and later became a Fa Yan(法眼), Fa Yin(法印) and Yu Chi(樂匙). He left about 15 texts, including "Su Wen Shao Shi"("素間紹識"), "Yi Xin Fang"("醫心方"), published in school, "Za Bing Guang Yao"("雜病廣要"), "Shang Han Guang Yao"(傷寒廣要), and "Zhen Fu Yao Jue"("該腹要訣"). On the Taki family's founding and working of the Yi Xue Guan Yasuka Doumei(失數道明) said they were "the people who took the initiative in Edo era kampo medicine" and evaluated their deeds in the fields of 'research of ancient text', 'the founding of Ji Shou Guan and medical education', 'publication business', 'writing of medical text'. 5. The doctors of the 'Kao Zheng Pai ' based their operations on the Edo Yi Xue Guan, and made groups with people with similar ideas to them, making a relationship 'net'. For example the three families of Duo Ji(多紀), Tang Chuan(湯川) and Xi Duo Cun(喜多村) married and adopted with and from each other and made prefaces and epitaphs for each other. Thus, the Taki family, the state science of the Makufu, the tendency of thinking, one's own interests and glory, one's own knowledge, the need of the society all played a role in the development of kampo medicine in the 18th and 19th century.

• 동국한의학연구소논문집
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• 제10권
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• pp.1-40
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• 2008

1.The 'Kao Zheng Pai'(考證派) comes from the 'Zhe Zhong Pai(折衷派)' and is a school that is influenced by the confucianism of the Qing dynasty. In Japan Inoue Kinga(井上金峨), Yoshida Koton(古田篁墩 $1745{\sim}1798$) became central members, and the rise of the methodology of historical research(考證學) influenced the members of the 'Zhe Zhong Pai', and the trend of historical research changed from confucianism to medicine, making a school of medicine based on the study of texts and proving that the classics were right. 2. Based on the function of 'Nei Qu Li'(內驅力) the 'Kao Zheng Pai', in the spirit of 'use confucianism as the base', researched letters, meanings and historical origins. Because they were influenced by the methodology of historical research(考證學) of the Qing era, they valued the evidential research of classic texts, and there was even one branch that did only historical research, the 'Rue Xue Kao Zheng Pai'(儒學考證派). Also, the 'Yi Xue Kao Zheng Pai'(醫學考證派) appeared by the influence of Yoshida Kouton and Kariya Ekisai(狩谷掖齋). 3. In the 'Kao Zheng Pai(考證派)'s theories and views the 'Yi Xue Kao Zheng Pai' did not look at medical scriptures like the "Huang Di Nei Jing"("黃帝內經") and did not do research on 'medical' related areas like acupuncture, the meridian and medicinal herbs. Since they were doctors that used medicine, they naturally were based on 'formulas'(方劑) and since their thoughts were based on the historical ideologies, they valued the "Shang Han Ja Bing Lun" which was revered as the 'ancestor of all formulas'(衆方之祖). 4. The lives of the important doctors of the 'Kao Zheng Pai' Meguro Dotaku(目黑道琢) Yamada Seichin(山田正珍), Yamada Kyoko(山田業廣), Mori Ritsi(森立之) Kitamura Naohara(喜多村直寬) are as follows. 1) Meguro Dotaku(目黑道琢 $1739{\sim}1798$) was born of lowly descent but, using his intelligence and knowledge, became a professor as a Shi Jing Yi(市井醫) and as a professor for 34 years at Ji Shou Guan(躋壽館) mastered the "Huang Di Nei Jing" after giving over 300 lectures. Since his pupil, Isawara Ken(伊澤蘭軒) taught the Lan Men Wu Zhe(蘭門五哲) and Shibue Chusai(澀江抽齋), Mori Ritsi(森立之), Okanishi Gentei(岡西玄亭), Kiyokawa Gendoh(淸川玄道) and Yamada Kyoko(山田業廣), Meguro Dotaku is considered the founder of the 'Yi Xue Kao Zheng Pai'. 2) The family of Yamada Seichin(山田正珍 $1749{\sim}1787$) had been medical officials in the Makufu(幕府) and the many books that his ancestors had left were the base of his art. Seichin learned from Shan Ben Bei Shan(山本北山), a 'Zhe Zhong Pai' scholar, and put his efforts into learning, teaching and researching the "Shang Han Lun"("傷寒論"). Living in a time between 'Gu Fang Pai'(古方派) member Nakanishi Goretada(中西惟忠) and 'Kao Zheng Pai' member Taki Motohiro(多紀元簡), he wrote 11 books, 2 of which express his thoughts and research clearly, the "Shang Han Lun Ji Cheng"("傷寒論集成") and "Shang Han Kao"("傷寒考"). His comparison of the 'six meridians'(3 yin, 3 yang) between the "Shang Han Lun" and the "Su Wen Re Lun"("素問 熱論") and his acknowledgement of the need and rationality of the concept of Yin-Yang and Deficient-Replete distinguishes him from the other 'Gu Fang Pai'. Also, his dissertation of the need for the concept doesn't use the theories of latter schools but uses the theory of the "Shang Han Lun" itself. He even researched the historical parts, such as terms like 'Shen Nong Chang Bai Cao'(神農嘗百草) and 'Cheng Qi Tang'(承氣湯). 3) The ancestor of Yamada Kyoko(山田業廣) was a court physician, and learned confucianism from Kao Zheng Pai's Ashikawa Genan(朝川善庵) and medicine from Isawa Ranken(伊澤蘭軒) and Taki Motokata(多紀元堅), and the secret to smallpox from Ikeda Keisui(池田京水). He later became a lecturer at the Edo Yi Xue Guan(醫學館) and was invited as the director to the Ji Zhong(濟衆) hospital. He also became the first owner of the Wen Zhi She(溫知社), whose main purpose was the revival of kampo, and launched the monthly magazine Wen Zi Yi Tan(溫知醫談). He also diagnosed and prescribed for the prince Ming Gong(明宮). His works include the "Jing Fang Bian"("經方辨"), "Shang Han Lun Si Ci"("傷寒論釋詞"), "Huang Zhao Zhu Jia Zhi Yan Ji Yao"("皇朝諸家治驗集要") and "Shang Han Ja Bing Lun Lei Juan"("傷寒雜病論類纂"). of these, the "Jing Fang Bian"("經方辨") states that the Shi Gao(石膏) used in the "Shang Han Lun" had three meanings-Fa Biao(發表), Qing Re(淸熱), Zi Yin(滋陰)-which were from 'symptoms', and first deducted the effects and then told of the reason. Another book, the "Jiu Zhe Tang Du Shu Ji"("九折堂讀書記") researched and translated the difficult parts of the "Shang Han Lun", "Jin Qui Yao Lue"("金匱要略"), "Qian Jin Fang"("千金方"), and "Wai Tai Mi Yao"("外臺秘要"). He usually analyzed the 'symptoms' of diseases but the composition, measurement, processing and application of medicine were all in the spectrum of 'analystic research' and 'researching analysis'. 4) The ancestors of Mori Ritsi(森立之 $1807{\sim}1885$) were warriors but he became a doctor by the will of his mother, and he learned from Shibue Chosai(澁江抽齋) and Isawaran Ken(伊澤蘭軒) and later became a pupil of Shou Gu Yi Zhai(狩谷掖齋), a historical research scholar. He then became a lecturer of medical herbs at the Yi Xue Guan, and later participated in the proofreading of "Yi Xin Fang"("醫心方") and with Chosai compiled the "Jing Ji Fang Gu Zhi"("經籍訪古志"). He visited the Chinese scholar Yang Shou Jing(楊守敬) in 1881 and exchanged books and ideas. Of his works, there are the collections(輯複本) of "Shen Nong Ben Cao Jing"("神農本草經") and "You Xiang Yi Hwa"("遊相醫話") and the records, notes, poems, and diaries such as "Zhi Yuan Man Lu"("枳園漫錄") and "Zhi Yuan Sui Bi"(枳園隨筆) that were not published. His thoughts were that in restoring the "Shen Nong Ben Cao Jing", "the herb to the doctor is like the "Shuo Wen Jie Zi"(說文解字) to the scholar", and he tried to restore the ancient herbal text using knowledge of medicine and investigation(考據), Also with Chosai he compiled the "Jing Ji Fang Gu Zhi"("經籍訪古志") using knowledge of ancient text. Ritzi left works on pure investigation, paid much attention to social problems, and through 12 years of poverty treated all people and animals in all branches of medicine, so he is called a 'half confucianist half doctor'(半儒半醫). 5) Kitamurana Ohira(喜多村直寬, $1804{\sim}1876$) learned scriptures and ancient texts from confucian scholar Asaka Gonsai(安積艮齋), and learned medicine from his father Huai Yaun(槐園), He became a teacher in the Yi Xue Guan in his middle ages, and to repay his country, he printed 266 volumes of "Yi Fang Lei Ju"("醫方類聚") and 1000 volumes of "Tai Ping Yu Lan"("太平禦覽") and devoted it to his country to be spread. His works are about 40 volumes including "Jin Qui Yao Lue Shu Yi"("金匱要略疏義") and "Lao Yi Zhi Yan"(老醫巵言) but most of them are researches on the "Shang Han Za Bing Lun". In his "Shang Han Lun Shu Yi"("傷寒論疏義") he shows the concept of the six meridians through the Yin-Yang, Superficial or internal, cold or hot, deficient or replete state of diseases, but did not match the names with the six meridians of the meridian theory, and this has something in common with the research based on the confucianism of Song(宋儒). In clinical treatment he was positive toward old and new methods and also the experience of civilians, but was negative toward western medicine. 6) The ancestor of the Taki family Tanbano Yasuyori(丹波康賴 $912{\sim}955$) became a Yi Bo Shi(醫博士) by his medical skills and compiled the "Yi Xin Fang"("醫心方"). His first son Tanbano Shigeaki(丹波重明) inherited the Shi Yao Yuan(施藥院) and the third son Tanbano Masatada(丹波雅忠) inherited the Dian You Tou(典藥頭). Masatada's descendents succeeded him for 25 generations until the family name was changed to Jin Bao(金保) and five generations later it was changed again to Duo Ji(多紀). The research scholar Taki Motohiro was in the third generation after the last name was changed to Taki, and his family kept an important part in the line of medical officers in Japan. Taki Motohiro(多紀元簡 $1755{\sim}1810$) was a teacher in the Yi Xue Guan where his father was residing, and became the physician for the general Jia Qi(家齊). He had a short temper and was not good at getting on in the world, and went against the will of the king and was banished from Ao Yi Shi(奧醫師). His most famous works, the "Shang Han Lun Ji Yi"("傷寒論輯義") and "Jin Qui Yao Lue Ji Yi"("金匱要略輯義") are the work of 20 years of collecting the theories of many schools and discussing, and is one of the most famous books on the "Shang Han Lun" in Japan. "Yi Sheng"("醫勝") is a collection of essays on research. Also there are the "Su Wen Shi"(素問識), "Ling Shu Shi"("靈樞識"), and the "Guan Ju Fang Yao Bu"("觀聚方要補"). Taki Motohiro(多紀元簡)'s position was succeeded by his third son Yuan Yin(元胤 $1789{\sim}1827$), and his works include works of research such as "Nan Jing Shu Jeng"(難經疏證), "Ti Ya"("體雅"), "Yao Ya"("藥雅"), "Ji Ya"(疾雅), "Ming Yi Gong An"(名醫公案), and "Yi Ji Kao"(醫籍考). The "Yi Ji Kao" is 80 volumes in length and lists about 3000 books on medicine in China before the Qing Dao Guang(道光), and under each title are the origin, number of volumes, state of existence, and, if possible, the preface, Ba Yu(跋語) and biography of the author. The younger sibling of Yuan Yin(元胤 $1789{\sim}1827$), Yuan Jian(元堅 $1795{\sim}1857$) expounded ancient writings at the Yi Xue Guan only after he reached middle age, was chosen for the Ao Yi Shi(奧醫師) and later became a Fa Yan(法眼), Fa Yin(法印) and Yu Chi(禦匙). He left about 15 texts, including "Su Wen Shao Shi"("素問紹識"), "Yi Xin Fang"("醫心方"), published in school, "Za Bing Guang Yao"("雜病廣要"), "Shang Han Guang Yao"("傷寒廣要"), and "Zhen Fu Yao Jue"("診腹要訣"). On the Taki family's founding and working of the Yi Xue Guan Yasuka Doumei(矢數道明) said they were "the people who took the initiative in Edo era kampo medicine" and evaluated their deeds in the fields of 'research of ancient text', the founding of Ji Shou Guan(躋壽館) and medical education', 'publication business', 'writing of medical text'. 5. The doctors of the 'Kao Zheng Pai' based their operations on the Edo Yi Xue Guan, and made groups with people with similar ideas to them, making a relationship 'net'. For example the three families of Duo Ji(多紀), Tang Chuan(湯川) and Xi Duo Cun(喜多村) married and adopted with and from each other and made prefaces and epitaphs for each other. Thus, the Taki family, the state science of the Makufu, the tendency of thinking, one's own interests and glory, one's own knowledge, the need of the society all played a role in the development of kampo medicine in the 18th and 19th century.