• 한국식품영양과학회지
• /
• 제34권7호
• /
• pp.1071-1076
• /
• 2005

방사선 조사식품의 지용성 비타민에 대한 안정성을 확인하기 위하여 지용성 비타민 중 $\beta-carotene$, 비타민 $D_3$, $\alpha-tocopherol$을 택하여 한국인의 상용 식품이면서 각각의 급원식품인 귤, 멸치, 쇠고기에 대해 방사선조사 전후의 해당 비타민 함량을 분석하였다. 귤의 $\beta-carotene$의 함량은 조사구가 비조사구에 비해 약간 증가하는 경향을 보였으나 유의적이지는 않았다. 멸치의 비타민 $D_3$ 함량은 조사선량에 비례하여 5kGy 조사 시 $109.30\%$, 10kGy 조사 시 $125.25\%$로 증가하였다. (p<0.05). 소고기의 $\alpha-tocopherol$ 함량은 고지방 부위인 등심육의 경우 조사선량에 따라 큰 폭으로 감소되어 비조사구에 비해 10kGy 조사구에서 $84\%$의 감소율을 보였으나, 지방함량이 비교적 적은 사태육의 경우 조사구 모두에서 비조사구 대비 약 $13\%$의 감소율을 보였다. 순수 용액의 비타민 A, 비타민 $D_3,\;\alpha-tocopherol$은 조사선량에 비례하여 함량이 감소하는 것으로 나타났으며 특히, 비타민 $D_3$는 지용성 비타민 중 조사에 가장 안정한 비타민으로 나타났고, $\alpha-tocopherol$의 경우는 10kGy 처리구에서 전량 감소를 보여 지용성 비타민 중 조사에 가장 취약한 비타민으로 나타났다. 마이크로웨이브 처리구나 가열 처리구에서는 이들 지용성 비타민들의 손실이 거의 없었다. 이사의 결과로부터 10kGy이내의 방사선 조사 시, 귤의 $\beta-carotene$ 함량에는 영향을 미치는 않는 것으로 보이며, 비타민 $D_3$의 경우는 적어도 10kGy까지는 매우 안정함을 보여주었고 지방함량이 높은 등심육의 경우 $\alpha-tocopherol$의 상당한 분해가 일어남을 알 수 있었다.

• Journal of Nutrition and Health
• /
• 제27권10호
• /
• pp.967-978
• /
• 1994

This study was done to investigate the effect of age and dietary linolenic acid content and the linolenic acid/linoleic acid(LNA/LA) ratio on the lipid metabolism and formation of PGI2 and TXA2. The male Sprague-Dawley rats were fed 6 different with 0.2, 0.4, 0.6 of LNA/LA ratio within either 8% LNA(high LNA) or 4% LNA(low LNA) of fatty acid content for different feeding period(1, 4, 12 month). The dietary fat used were sesame oil, perilla oil, soybean oil and beef tallow. The concentration of serum total lipid, total cholesterol and HDL-C were increased with aging. Triglyceride concentration was decreased in 0.2 ratio of LNA/LA. The lipid content of liver showed similar tendency to that of serum. The ratio of PGI2/TXA2 was increased in 1 month rats and antithrombotic effect was reduced significantly with increasing age. The PGI2/TXA2 ratio was tended to be higher in diet of 0.2 and 0.4 LNA/LA ratio at high LNA level and in diet of 0.6 LNA/LA ratio at low LNA level. Especially PGI2/TXA2 ratio was increased linearly with rising LNA/LA ratio at low LNA level. It seemed that the LNA content and LNA/LA ratio had interaction to increase the antithrombotic effect bychanging TXA2 synthesis. And the dietary fatty acid related effect lowering the serum and liver lipid content, excepting triglyceride, was increased when dietary n3/n6 ratio was high(0.6) at both high and low n3 level. Therefore, it could not be recommended to consume large amount of n3 fatty acid or high ratio of n3/n6 to prevent cardiovascular diseases. These results suggested that the dietary fatty acid ratio of n3/n6 could be determined based on the n-3 content of dietary fat to reduce the risk of cardiovascular disease.

• Asian-Australasian Journal of Animal Sciences
• /
• 제27권11호
• /
• pp.1652-1662
• /
• 2014

The present study investigated the optimum blending condition of protected fat, choline and yeast culture for lowering of rumen temperature. The Box Benken experimental design, a fractional factorial arrangement, and response surface methodology were employed. The optimum blending condition was determined using the rumen simulated in vitro fermentation. An additive formulated on the optimum condition contained 50% of protected fat, 25% of yeast culture, 5% of choline, 7% of organic zinc, 6.5% of cinnamon, and 6.5% of stevioside. The feed additive was supplemented at a rate of 0.1% of diet (orchard grass:concentrate, 3:7) and compared with a control which had no additive. The treatment resulted in lower volatile fatty acid (VFA) concentration and biogas than the control. To investigate the effect of the optimized additive and feed energy levels on rumen and rectal temperatures, four rumen cannulated Hanwoo (Korean native beef breed) steers were in a $4{\times}4$ Latin square design. Energy levels were varied to low and high by altering the ratio of forage to concentrate in diet: low energy (6:4) and high energy (4:6). The additive was added at a rate of 0.1% of the diet. The following parameters were measured; feed intake, rumen and rectal temperatures, ruminal pH and VFA concentration. This study was conducted in an environmentally controlled house with temperature set at $30^{\circ}C$ and relative humidity levels of 70%. Steers were housed individually in raised crates to facilitate collection of urine and feces. The adaptation period was for 14 days, 2 days for sampling and 7 days for resting the animals. The additive significantly reduced both rumen (p<0.01) and rectal temperatures (p<0.001) without depressed feed intake. There were interactions (p<0.01) between energy level and additive on ruminal temperature. Neither additive nor energy level had an effect on total VFA concentration. The additive however, significantly increased (p<0.01) propionate and subsequently had lower acetate:propionate (A/P) ratios than non-additive supplementation. High concentrate diets had significantly lower pH. Interactions between energy and additive were observed (p<0.01) in ammonia nitrogen production. Supplementation of diets with the additive resulted in lower rumen and rectal temperatures, hence the additive showed promise in alleviating undesirable effects of heat stress in cattle.

• Journal of Nutrition and Health
• /
• 제17권4호
• /
• pp.297-304
• /
• 1984

다섯 종류의 실험유지로 고등어유, 장어유, 콩기름, 채종유 및 쇠기름을 사용하여 먹이의 10%(w/w)되는 조제식이를 2주간 휜쥐에게 시행하여, 혈장, 심장, 지방조직의 lipoprotein lipase의 활성도, mitochondrial respiration측정하고, mitochondrial membrane matrix 지질의 지방산구성을 조사하였다. Lipoprotein lipase의 활성은 혈장에서는 쇠기름군이 식물유 및 어유군에 비해 약 60%정도 높고, 지방조직에서는 거의 3배가량 높았으나, 식물유와 어유군 간에는 차이가 없었다. 각 군간에 mitochondrial respiration율은 심장조직에서는 차이가 별로 없었고, 채종유군이 P/O ratio가 심장, 간장조직에서 모두 낮았고 어유(魚油)군의 간장조직mitochondrial respiration이 타조직에 비해 낮았다. Mitochondrial inner membrane matrix분획의 지질 지방산조성은 콩기름군에서 $C_{18:2}$, $C_{20:4}$ 함량이 높고 고등어유군은$C_{22:1}$가 간장조직에서 발견되는 반면 채종유군은 동일 지방산이 심장 mitochondria에서 발견되었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권2호
• /
• pp.293-300
• /
• 2018

Objective: This study observed the effects of cooking method and final core temperature on cooking loss, lipid oxidation, aroma volatiles, nucleotide-related compounds and aroma volatiles of Hanwoo brisket (deep pectoralis). Methods: Deep pectoralis muscles (8.65% of crude fat) were obtained from three Hanwoo steer carcasses with $1^+$ quality grade. Samples were either oven-roasted at $180^{\circ}C$ (dry heat) or cooked in boiling water (moist heat) to final core temperature of $70^{\circ}C$ (medium) or $77^{\circ}C$ (well-done). Results: Boiling method reduced more fat but retained more moisture than did the oven roasting method (p<0.001), thus no significant differences were found on cooking loss. However, samples lost more weight as final core temperature increased (p<0.01). Further, total saturated fatty acid increased (p = 0.02) while total monounsaturated fatty acid decreased (p = 0.03) as final core temperature increased. Regardless the method used for cooking, malondialdehyde (p<0.01) and free iron contents (p<0.001) were observed higher in samples cooked to $77^{\circ}C$. Oven roasting retained more inosinic acid, inosine and hypoxanthine in samples than did the boiling method (p<0.001), of which the concentration decreased as final core temperature increased except for hypoxanthine. Samples cooked to $77^{\circ}C$ using oven roasting method released more intense aroma than did the others and the aroma pattern was discriminated based on the intensity. Most of aldehydes and pyrazines were more abundant in oven-roasted samples than in boiled samples. Among identified volatiles, hexanal had the highest area unit in both boiled and oven-roasted samples, of which the abundance increased as the final core temperature increased. Conclusion: The boiling method extracted inosinic acid and rendered fat from beef brisket, whereas oven roasting intensified aroma derived from aldehydes and pyrazines and prevented the extreme loss of inosinic acid.

• 한국지역사회생활과학회지
• /
• 제28권3호
• /
• pp.403-413
• /
• 2017

This study was performed to investigate the quality characteristics, including sensory and physical properties, of hamburger patties added with spinach as a source of fiber. Hamburger patties were prepared with spinach at five different levels (5, 10, 15, 20, and 25%. Moisture contents of the groups with spinach were significantly higher than that of the control group. Crude fat and crude protein contents of the control group were higher than those of the group with spinach. Crude ash content of the control group was the lowest among the groups. No significant difference in pH was observed among the groups. The cooking loss rates of groups with spinach were significantly reduced compared to that of the control group. No significant difference in L value was observed among the groups. However, a value of the control group was higher than those of the groups with spinach. Otherwise, b value of the control group was lower than those of the groups with spinach. The hardness levels of the control groups were significantly higher than those of groups with spinach. In the quantitative descriptive analysis, green color and bitter taste of the groups with spinach were stronger than that of the control group. The preference levels for appearance of the groups with 10 to 15% spinach were higher than those of the other groups, including the control groups. Overall acceptabilities of the groups with 5 to 15% spinach were significantly higher than those of the other groups. In conclusion, spinach can increase the acceptability of hamburger patties, and 10 to 15% would appear to be the proper added amount.

• Journal of Nutrition and Health
• /
• 제39권1호
• /
• pp.3-10
• /
• 2006

Effects of garlic powder supplementation on blood lipid profile and antioxidant system were investigated in rats with and without swimming exercise. Sprague-Dawley rats of four experimental groups were fed for 4 weeks diets containing $15\%$ beef tallow and $1\%$ cholesterol; control without garlic and exercise, Go with $2\%$ garlic alone, Ex with exercise alone, GoEx with $2\%$ garlic and exercise. Rats were trained 40 min a days a days a week. Group Ex and GoEx showed significant lowering in body weight gain and fat accumulation. In Go, Ex and GoEx, plasm TG and LDL-C were lower and HDL-C was higher, although not significantly, compared to levels in control. Total cholesterol was significantly reduced in group Go, and Ex and GoEx were lower than control. The total/HDL cholesterol ratio was also found to be significantly different, decreasing the ratios in Go, Ex and GoEx. The hepatic TBARS increased significantly in group Ex $(51.7{\pm}3.43nM/g\;liver)$, while TBARS in Go and GoEx were low $(35.68{\pm}3.61,\;39.30{\pm}5.55nM/g\;liver)$ and similar to control's one. The activity of hepatic SOD in Go and GoEx tended higher than control and Ex without garlic. The hepatic catalase showed significantly the highest activity in Go. Activity of GSH-px was significantly low in Ex with $0.14{\pm}0.03$ unit/mg protein, and control, Go and GoEx had higher activities of $0.23{\pm}0.08,\;0.20{\pm}0.07,\;0.22{\pm}0.01\;unit/mg$ protein, respectively. Lower activities of antioxidant enzymes in Ex are likely to associated with the highest level of TBARS. It seems that a decrease in TBARS in GoEx relative to Ex was related to the increase in GSHpx and SOD with garlic supplemented, which led to compensate the oxidative stress from exercise. The results suggests that exercise or garlic supplement exerts blood lipid attenuating effect. In adition, garlic supplementation could strengthen the antioxidant potential against exercise-induced oxidants, partly by modulating oxidant enzyme activity. These effects of garlic may make it a beneficial agent on CVD.

• Journal of Nutrition and Health
• /
• 제17권4호
• /
• pp.290-296
• /
• 1984

어유식이(魚油食餌)가 간장조직내 lipogenic enzyme의 활성도에 미치는 영향을 조사하고자 고등어유를 식이(食餌) 10%(w/w) 되게 먹이를 조제하여 13일동안 사육한 흰쥐의 간장조직의 glucose-6-phosphate dehydrogenase와 malic enzyme의 활성도는 lard를 섭취한 군(群)보다 $3{\sim}6$일의 섭취로 훨씬 저하되었으며 콩기름에 비하여는 같거나 또는 약간 낮은 값을 보였다. 고등어 또는 장어유를 어유(魚油)로 사용하여 먹이의 $0{\sim}10%$로 함량을 변화시키며 총지방량을 10%로 일정히 하고자 콩기름 또는 쇠기름을 배합유로 사용하였을 때 glucose-6-phosphate dehydrogenase의 활성은 어유(魚油)가 2 % 포함하였을 때부터 malic enzyme의 활성은 5% 포함하였을 때, 어유(魚油)를 포함하지 않은 군(群)에 비하여 감소를 보였다.

• Journal of Animal Science and Technology
• /
• 제51권3호
• /
• pp.231-240
• /
• 2009

본 연구는 반추위 캐뉼라가 장착된 거세한우 16두 ($626.2\pm47.72$ kg)를 이용하여 한우 복강 및 피하지방 감소 항체를 생체 주사 처리 시 사료섭취량, 반추위 발효패턴 및 혈액 대사물질 등 영양생리대사에 미치는 영향을 조사하고자 실시하였다. Choi 등(2008)에서 개발된 한우복강(AAb) 및 피하지방 감소 항체(SAb)을 피하 주사하였을 때, 주사 후 2주차에서 수치적으로 체중의 감소가 발생했으나, 무처리구 및 비면역항체처리구에서도 체중의 감소가 발생해 항체 주사에 의한 처리의 영향이라기 보다는 다른 요인(혈액채취 등)에 의한 스트레스에서 기인하는 것으로 판단된다. 항체 주사 처리 시 반추위 pH 패턴, 휘발성 지방산 및 암모니아 농도에는 유의적 변화(P > 0.05)가 나타나지 않았고, 그 수준 또한 일반적인 수준 범위에서 벗어나지 않았다. 혈액 대사물질의 변화에서는 항체 주사 처리 전 BUN 농도에서 무처리구 기준, AAb 처리구는 낮게, 반면에 SAb 처리구는 높게 나타났으나(P < 0.05), 오히려 항체 주사 처리 후에서 유의적 차이가 나타나지 않았다(P > 0.05). 혈액 내 glucose 농도 역시 항체 주사 처리 전 비면역혈청처리구에서만 유의적 차이를 보였고(P < 0.05), 항체 주사 처리 후에서는 처리구별 유의적 영향은 없었다. 혈액 내 중성지방의 농도는 항체 주사 처리에 의한 유의적 차이는 나타나지 않았고, 그 농도도 11.4~19.9 mg/$d{\ell}$로 일반적인 농후사료 위주의 사료 급여시 거세한우의 혈액 내 중성지방의 수준으로 판단되었다. 따라서, 이상의 결과를 볼 때, 본 연구에서 이용된 AAb 및 SAb는 in vivo 영양생리대사에 부정적 영향을 미치지 않는 안전한 항체로 사료된다.

• Journal of Nutrition and Health
• /
• 제20권2호
• /
• pp.111-121
• /
• 1987

어유를 섭취하였을 때, 체내 과산화물 생성과 항산화 기능을 조사하며, 그 결과를 다른 식이지방과 비교하기 위하여, 고등어유, 대두유, 쇠기름, 들기름, 채종유의 5 종의 지방을 먹이의 10% 되게 식이를 조제하여 70g 내외의 암, 수컷의 흰쥐에게 섭취시켰다. 출생후 초기 성장시에 적응도를 관찰하기 위하여, 위의 쥐들을 34일간 조제식이로 사육한후, 교배시켜 출생한 2 대째 쥐들을 수유시기(17일, 26일)과 이유시기(39일)에 희생시키고, 출산, 수유를 마친 어미쥐(나이 123일, 총 식이일수 81일)를 희생하여 간과 뇌조직의 지질과산화물, $\alpha$-tocopherol, glutathione 양을 정량하고, glutathione peroxidase, superoxide dismutase의 활성을 측정하였다. 간조직의 지질과산화물 값은 고등어유를 섭취한 어미쥐와 수유기(17, 26일)의 새끼쥐들에게서 타군에 비해 높았으나 39일의 새끼쥐에서는 채종유보다는 높았으나 대두유, 들기름군과 같은 수준이었다. 뇌조직에서는 간에 차이가 거의없었다. 간조직의 $\alpha$-tocopherol 농도와 환원형의 glutathione(GSH) 의 농도가 어미쥐에서 타군에 비해 현저히 낮았고, 새끼쥐에서도 $\alpha$-tocopherol 경우는 같은경향이나 그 정도가 덜 현저하며, GSH의 경우는 타군들과 차이가 없었다. 산화형 glutathioe (GSSG)의 농도는 어미쥐, 새끼쥐모두에서 식이지방에 따른 차이가 일관성 있게 나타나지 않았다. Glutathione peroxidase 활성은 간장과 뇌조직에서 성장과정중의 새끼쥐들에서 모두 약간씩 증가하였고, 채종유군에서 타군에 비해 증가정도가 낮아 활성이 39일에 현저히 낮았다. Superoxide dismutase 의 활성은 성장기간에 따른 변화는 적었고, 고등어유군이 타군에 비해 비교적 낮은값을 유지하였다. 어미쥐와 17일째 새끼쥐에서는 달리 26일과 39일째 새끼쥐의 간조직 glutathioe peroxidase 의활성이 지질과산화물 농도와 정의 상관관계가 보여져, 출생후 초기 성장과정에서 체내 적응성이 존재함을 나타내주었다.