• Journal of Microbiology and Biotechnology
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• v.1 no.3
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• pp.188-196
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• 1991

The ${\beta}-glucosidase$ was purified to homogeneity from the culture filtrate of P. verruculosum by column chromatography. The enzyme was a glycoprotein with a relative size of approximately 220 kDa with an isoelectric point of 4.8, which was composed of dimeric protein of 105 kDa. The enzyme was stable up to $60^{\circ}C$ and the presence of glycerol significantly increased its thermostability. The enzyme was found to hydrolyze both ${\beta}-aryl$ and ${\beta}-alkyl-glucosides$ in addition to ${\beta}-glucosyl$ glucose and catalyzed glucosyl transfer to cellobiose. The enzyme attacked laminarin in an exotype-like fashion. The apparent Km's of the enzyme toward cellobiose, laminaribiose, laminarin were 0.53 mM, 0.35 mM and 1.11 mM, respectively. Glucose and glucono-${\delta}-lactone$ were competitive inhibitors for the enzyme. Copper ($Cu^{2+}$), mercury ($Hg^{2+}$) and p-chloromercuribenzoate were strong inhibitors of the enzyme. The immunoblotting result revealed that one form of ${\beta}-glucosidase$ was biosynthesized, irrespective of carbon sources used. Polyacrylamide gel electrophoresis analysis of the in vitro translated product of total RNA from avicel grown mycelium established that the P. verruculosum ${\beta}-glucosidase$ precursor was approximately 95 kDa in size. The amino acid composition and N-terminal amino acid sequence are given.

• Journal of KIISE:Databases
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• v.34 no.6
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• pp.473-482
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• 2007

Network-based IDS(Intrusion Detection System) gathers network packet data and analyzes them into attack or normal. They raise alarm when possible intrusion happens. But they often output a large amount of low-level of incomplete alert information. Consequently, a large amount of incomplete alert information that can be unmanageable and also be mixed with false alerts can prevent intrusion response systems and security administrator from adequately understanding and analyzing the state of network security, and initiating appropriate response in a timely fashion. So it is important for the security administrator to reduce the redundancy of alerts, integrate and correlate security alerts, construct attack scenarios and present high-level aggregated information. False alarm rate is the ratio between the number of normal connections that are incorrectly misclassified as attacks and the total number of normal connections. In this paper we propose a false alarm classification model to reduce the false alarm rate using classification analysis of data mining techniques. The proposed model can classify the alarms from the intrusion detection systems into false alert or true attack. Our approach is useful to reduce false alerts and to improve the detection rate of network-based intrusion detection systems.

• Journal of Korean Neurosurgical Society
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• v.48 no.3
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• pp.244-250
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• 2010

Objective : Adequate management of increased intracranial pressure (ICP) is critical in patients with traumatic brain injury (TBI), and decompressive craniectomy is widely used to treat refractory increased ICP. The authors reviewed and analyzed complications following decompressive craniectomy for the management of TBI. Methods : A total of 89 consecutive patients who underwent decompressive craniectomy for TBI between February 2004 and February 2009 were reviewed retrospectively. Incidence rates of complications secondary to decompressive craniectomy were determined, and analyses were performed to identify clinical factors associated with the development of complications and the poor outcome. Results : Complications secondary to decompressive craniectomy occurred in 48 of the 89 (53.9%) patients. Furthermore, these complications occurred in a sequential fashion at specific times after surgical intervention; cerebral contusion expansion ($2.2{\pm}1.2$ days), newly appearing subdural or epidural hematoma contralateral to the craniectomy defect ($1.5{\pm}0.9$ days), epilepsy ($2.7{\pm}1.5$ days), cerebrospinal fluid leakage through the scalp incision ($7.0{\pm}4.2$ days), and external cerebral herniation ($5.5{\pm}3.3$ days). Subdural effusion ($10.8{\pm}5.2$ days) and postoperative infection ($9.8{\pm}3.1$ days) developed between one and four weeks postoperatively. Trephined and post-traumatic hydrocephalus syndromes developed after one month postoperatively (at $79.5{\pm}23.6$ and $49.2{\pm}14.1$ days, respectively). Conclusion : A poor GCS score ($\leq$ 8) and an age of $\geq$ 65 were found to be related to the occurrence of one of the above-mentioned complications. These results should help neurosurgeons anticipate these complications, to adopt management strategies that reduce the risks of complications, and to improve clinical outcomes.

• YAKHAK HOEJI
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• v.52 no.1
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• pp.73-78
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• 2008

Immune system can protect host attacking from a variety of microorganism and virus through innate and adaptive immunities. The innate immune system can be activated by recognition of conserved carbohydrates on the cell surface of pathogen resulting in protection, immunity regulation and inflammation. Immunostimulating and anti-tumor ${\beta}$-glucan, major cell wall component of many fungi, could be recognized as pathogen associated molecular pattern (PAMP) by C-type lectin such as pathogen recognition receptor (PRR) of host innate immunity cells. In spite of many studies of basidiomycetes ${\beta}$-glucan on immunostimulation, little is known about the precise mechanism as molecular-level. Among C-type lectins, dectin-1 was cloned and reported as a ${\beta}$-glucan receptor. In this report, we demonstrated induction of cytokine gene transcription by Ganoderma lucidum ${\beta}$-glucan in the absence or presence of lipopolysaccharide (LPS) by RT-PCR analysis. The expression of murine dectin-1 (MD-1) on RAW264.7 macrophage by RT-PCR showing both the full length, 757 bp $(MD-1{\alpha})$ and alternative spliced form, 620 bp $(MD-1{\beta})$. Both $MD-1{\alpha}$ and $MD-1{\beta}$ mRNAs were induced by ${\beta $-glucan both in the absence and presence of LPS. To explore expression of inflammatory cytokines by ${\beta}$-glucan, RAW264.7 cells were treated with ${\beta}$-glucan for 12 hours. As a result, the expressions of IL-1 IL-6, IL-l0 and $TNF-{\alpha}$ were increased by ${\beta}$-glucan treatment in a dose-dependent fashion. From these results, ${\beta}$-glucan induced transcriptions of dectin-1 and immune activating cytokine genes, indicating induction of immune allertness by expressing dectin-1 and secreting inflammatory cytokines.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.19 no.2
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• pp.26-39
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• 2006

Objective : The effect of water extract of Chungjogupae-tang (CJGPT) was investigated on the growth of human lung carcinoma A549 cells. Methods : MTT assay and fluorescent microscope performed to compare and examine the efficacy of CJGPT treatment on the cytostaticity of lung cancer cells in proportion to time and doses, and DAPI staining and Western blot analysis were used to examine their effect on apoptosis. In addition the quantitative RT-PCR was used to examine to lung cancer cells growth and Progtaglandin E2 and Telomerase activity were measured Results : Exposure of A549 cells to CJGPT resulted in the growth inhibition and apoptosis in a dose-dependent manner as measured by MTT assay and fluorescent microscope. The antiuoliferative effect by CJGPT treatment in A549 cells was associated with morphological changes such as membrane shrinking and cell rounding up. CJGPT treatment resulted in an up-regulation of cyclin-dependent kinase inhibitor p21(WAF1/CIPl) in a p53-independent fashion. We found that CJGPT treatment decreased the levels of cyclooxygenase (COX)-2 and inducible nitric oxide synthease (iNOS) expression without significant changes in the expression of COX-1, which was correlated with a decrease in protaglandin E2 (PGE2) synthesis. CJGPT treatment also inhibited the levels of human telomerase reverse transcriptase (hTERT) and telomerase-associated protein (TEP)-1 mRNA expression, however the activity of telomerase was slightly increased by CJGPT treatment. Conclusion : These findings suggested that CJGPT-induced inhibition of human lung carcinoma A549 cell growth was connected with the induction of apoptotic cell death and the results provided important new insights into the possible molecular mechanisms of the anti-cancer activity of CJGPT.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.2939-2946
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• 2015

Many of the anti-cancer agents currently used have an origin in natural sources including plants. Aloe vera is one such plant being studied extensively for its diverse health benefits, including cancer prevention. In this study, the cytotoxic potential of Aloe vera crude extract (ACE) alone or in combination with cisplatin in human breast (MCF-7) and cervical (HeLa) cancer cells was studied by cell viability assay, nuclear morphological examination and cell cycle analysis. Effects were correlated with modulation of expression of genes involved in cell cycle regulation, apoptosis and drug metabolism by RT-PCR. Exposure of cells to ACE resulted in considerable loss of cell viability in a dose- and time-dependent fashion, which was found to be mediated by through the apoptotic pathway as evidenced by changes in the nuclear morphology and the distribution of cells in the different phases of the cell cycle. Interestingly, ACE did not have any significant cytotoxicity towards normal cells, thus placing it in the category of safe chemopreventive agent. Further, the effects were correlated with the downregulation of cyclin D1, CYP 1A1, CYP 1A2 and increased expression of bax and p21 in MCF-7 and HeLa cells. In addition, low dose combination of ACE and cisplatin showed a combination index less than 1, indicating synergistic growth inhibition compared to the agents applied individually. In conclusion, these results signify that Aloe vera may be an effective anti-neoplastic agent to inhibit cancer cell growth and increase the therapeutic efficacy of conventional drugs like cispolatin. Thus promoting the development of plant-derived therapeutic agents appears warranted for novel cancer treatment strategies.

• The International Journal of Costume Culture
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• v.3 no.1
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• pp.1-11
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• 2000

The purpose of this study is to compare the constitutions comparatively among Korean, Chinese and Chosunjok college women and thereby, provide for some basic data helpful to the development of export apparels befitting the Chinese consumers, For this purpose, Korean, Chinese and Chosunjok college women aged between 17 and 24 were sampled to be subject to body measurements. The measurement data obtained were indexed for each part of the body to set a total 29 constitutional indices, which were analyzed by factor dictating the major shapes of body, Thus, based on the constructed, the differences of constitution among three groups were comparatively analyzed. The results of this study of this study can be summarized as follows; It was found through this study that Korean college women had thickest neck compared with body size and had longer lower limbs compared with stature, Moreover, their lower body was absolutely longer than the other two groups, and thus, Korean college women were found slimmest. On the other hand, Chinese college women had most voluminous chest turned over with shorter and thicker body. they were obesest. Chosunjok college women had longest upper body but shortest breast length. Their lower body was obesest, and their body was longer compared with stature. Moreover, Chosunjok college women's lower limbs were shortest, while their arm scyes were small nearest body type.

• The International Journal of Costume Culture
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• v.13 no.2
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• pp.104-117
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• 2010

The purpose of this study is to find out the effect of on-the-job experiencing program on the formation of dynamics such as creativity, organizing, and clarity necessary for launching into the society through a cause-and-effect analysis to determine the priority of resources investments and improvements of educational courses. As a result, the selling experience and planned workshop were found to have effect on the creative dynamic that preparatory business starters must secure without fail. This means that e-Fashionn Lab(http://www.bc2d.com) which is an experiencing program, is fully accomplishing its initial objective set up at the time of the system design. In addition, the selling experience and the mini-home page experience were found to have effect on the formation of organizing dynamic of preparatory business starters. This shows that the system design to realize the database of personal friendships and personal information is being used fully as an educational practice instruments. Lastly, the selling experience and planned workshops were found to have effect on the formation of clarity dynamic of preparatory business starters as experiencing characteristic has effect on creativity. However, the community (work room) experience was found to have no effect on the formation of dynamics necessary for preparatory business starters to advance into the society at this study. This signifies that the system input resources should be pushed back in priority and that curriculums and system design should be concentrated on strengthening the selling experience and planned workshop in general.

• Journal of the Korea Society of Computer and Information
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• v.23 no.7
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• pp.63-72
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• 2018

The purpose of this study was to compare and analyze the pants pattern of the lower body of 70-85 aged women. I tried to present excellent pants pattern that is convenient and suitable for the activities of elderly women. Through this, I tried to provide basic data necessary for making elderly women 's pants. As a result of evaluating the appearance of the pants pattern according to the body type, the fit of the type 2 was evaluated as the highest in the item except the position of the front waist line. For the objective evaluation of the 3D simulation, the air gap of the pants by the body part analysis showed that there was not much difference in the air gap around the waist circumference, hip circumference, thigh circumference, and knee circumference by applying the same drawing method and body size. However, in type 2, the air gap of thigh and knee circumference parts was larger than that of type 1 and type 3. Because type 2's legs were thin but it used same size of hemline. It was thought that it is necessary to adjust the space of front and back crotch length to 1 inch (2.54 cm) instead of 1.9 cm (3/4 inch) so that the waistline position of all three types can be set up a little to cover the abdomen. This study was conducted to investigate changes in body shape of elderly women and to develop appropriate pants patterns.

• Journal of Korean Home Economics Education Association
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• v.12 no.1
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• pp.75-87
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• 2000

The purpose of this study is to find out how middle and high school girls show their clothing attitudes and their life-style. clothing behavior. use their ornaments according to demographic variables. The subject of this study consists o 345 middle and high school girls living in Chung-Nam Province and Taejon Metropolitan City. SPSS/PC+ program was utilized to calculate frequency(N) mean value(M) and standard deviation (SD) for statistical analysis. Differences among the populations are examined through t-test. F-test and X2-test. The results of this study are as follow : 1. Correlation between the life style and demographic variables. Especially. life-style has significant difference(p<. 05) according to type of school. middle school girls have high tendency in the type of modern and fashion. Life-style has significant difference(p< .01) according to level of achievement studying, high level girls has high tendency in the type of practical and secure. 2. Correlation between the clothing behavior and demographic variables. According to type of school. middle school girls showed higher than high school girls in clothing conformity and clothing aesthetics. According to level of achievement studying, high level girls have high tendency in clothing conformity and clothing modesty. 3. Correlation between the use their ornaments and demographic variables. Buying the ornaments, higher-level achievement studying girl’s get information in show window. but lower-level achievement studying girl’s get information in TV or radio. The use of ornaments according to the site of school, most of school girls who live in small city or town use the ornaments as usual school life but school girls who live in big city use the ornaments to look good on their clothes. Following suggestions can be made based on the discussions above: It is necessary for us to have correct understanding about the correlation between the ornaments and clothing of school girls in order to lead them to have appropriate clothing behavior. Furthermore. it is required to construct a bridge linking a course of study to rational habits guidance which will provide us more appropriate tools for clothing behavior guidance.