• Journal of Ginseng Research
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• 제46권2호
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• pp.235-247
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• 2022

Background: Ginsenoside Rg3 is one of the main active ingredients in ginseng. Here, we aimed to confirm its protective effect on the heart function in transverse aortic coarctation (TAC)-induced heart failure mice and explore the potential molecular mechanisms involved. Methods: The effects of ginsenoside Rg3 on heart and mitochondrial function were investigated by treating TAC-induced heart failure in mice. The mechanism of ginsenoside Rg3 for improving heart and mitochondrial function in mice with heart failure was predicted through integrative analysis of the proteome and plasma metabolome. Glucose uptake and myocardial insulin sensitivity were evaluated using micro-positron emission tomography. The effect of ginsenoside Rg3 on myocardial insulin sensitivity was clarified by combining in vivo animal experiments and in vitro cell experiments. Results: Treatment of TAC-induced mouse models with ginsenoside Rg3 significantly improved heart function and protected mitochondrial structure and function. Fusion of metabolomics, proteomics, and targeted metabolomics data showed that Rg3 regulated the glycolysis process, and Rg3 not only regulated glucose uptake but also improve myocardial insulin resistance. The molecular mechanism of ginsenoside Rg3 regulation of glucose metabolism was determined by exploring the interaction pathways of AMPK, insulin resistance, and glucose metabolism. The effect of ginsenoside Rg3 on the promotion of glucose uptake in IR-H9c2 cells by AMPK activation was dependent on the insulin signaling pathway. Conclusions: Ginsenoside Rg3 modulates glucose metabolism and significantly ameliorates insulin resistance through activation of the AMPK pathway.

• Parasites, Hosts and Diseases
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• 제59권6호
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• pp.625-634
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• 2021

Based on the field investigations in 91 investigation sites (counties) in southwest China between 2001 and 2019, the present paper reported the chigger mites on A. agrarius mice in southwest China for the first time by using a series of statistical methods. From 715 striped field mice captured in 28 of 91 investigated sites, only 255 chiggers were collected, and they were identified as 14 species, 6 genera in 3 subfamilies under 2 families. Of 715 A. agrarius mice, only 24 of them were infested with chigger mites with low overall prevalence (P_M=3.4%), overall mean abundance (M_A=0.36 mites/host) and overall mean intensity (M_I=10.63 mites/host). The species diversity and infestation of chiggers on A. agrarius were much lower than those previously reported on some other rodents in southwest China. On a certain species of rodent, A. agrarius mouse in southwest China seems to have a very low susceptibility to chigger infestations than in other geographical regions. Of 14 chigger species, there were 3 dominant species, Leptotrombidium sialkotense, L. rupestre and Schoengastiella novoconfuciana, which were of aggregated distribution among different individuals of A. agrarius hosts. L. sialkotense, one of 6 main vectors of scrub typhus in China, was the first dominant on A. agrarius. The species similarity of chigger mites on male and female hosts was low with C_SS=0.25, and this reflects the sex-bias of different genders of A. agrarius mice in harboring different chigger species.

• Parasites, Hosts and Diseases
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• 제61권3호
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• pp.272-281
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• 2023

Chigger mites are the vector of scrub typhus. This study estimates the infestation status and ecological characteristics of chiggers on the chestnut white-bellied rat Niviventer fulvescens in Southwest China between 2001 and 2019. Chiggers were identified under the microscope, and infestation indices were calculated. The Preston's log-normal model was used to fit the curve of species abundance distribution. A total of 6,557 chiggers were collected in 136 of 342 N. fulvescens rats, showing high overall infestation indices (prevalence=39.8%, mean abundance=19.2, mean intensity=48.2) and high species diversity (S=100, H'=3.0). Leptotrombidium cangjiangense, Neotrombicula japonica, and Ascoschoengastia sifanga were the three dominant chigger species (constituent ratio=42.9%; 2,736/6,384) and exhibited an aggregated distribution among different rat individuals. We identified 100 chigger species, with 3 of them (Leptotrombidium scutellare, Leptotrombidium wenense, and Leptotrombidium deliense) as the main vectors of scrub typhus in China and nine species as potential vectors of this disease. Disease vector occurrence on N. fulvescens may increase the risk of spreading scrub typhus from rats to humans. Chigger infestation on N. fulvescens varied significantly in different environments. The species abundance distribution showed a log-normal distribution pattern. The estimated number of chigger species on N. fulvescens was 126 species.

• 한국엔터테인먼트산업학회논문지
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• 제13권3호
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• pp.13-25
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• 2019

방탄소년단은 2018년 5월 LOVE YOURSELF 전(轉)Tear로 빌보드 메인 앨범 차트 '빌보드 200' 정상을 처음 밟은 후 3개월 만에 다시 Repackage 앨범 LOVE YOURSELF 결(結) ANSWER(2018년 8월 24일 발매)로 '빌보드 200' 1위(2018년 9월 3일)에 올랐다. 영어가 아닌 한국어 가사로 빌보드 메인 차트를 두번씩이나 정복하며 '한류 4.0시대'를 열었다. 방탄소년단은 '빌보드 200' 정상에 두 번이나 오름으로써 그들의 음악적 재능(노래, 가무, 프로모션 등)을 전 세계적으로 공인받고 글로벌 주류 음악 시장에 안착하게 되었다. 방탄소년단은 신비주의, 이상성, 파격 등 직관적 상호작용을 탈피하고 공감, 공유, 공생 등 양방향 소통을 통해 지구촌 팬들과 하나의 계관(BTS Universe)을 구축하고 있다. 단지 몇 개의 히트곡을 발표한 인기 그룹에 그치지 않고 대중음악을 예술적 가치로 승화시킨 아티스트로서의 권위를 인정받고 있다. 그 결과 충성도 높은 글로벌 팬덤(A.R.M.Y.)을 갖게 되었고 그들과 함께 선한 영향력을 지속적으로 만들어내고 있다. 본 논문은 다음과 같이 방탄소년단의 성공 요인을 S-M-C-R-E 모델을 통해 분석했다. ① Sender(송신자) : 방탄소년단의 7인 7색 판타지와 방시혁 프로듀서의 '올인원(All in one) 스토리텔링' 전략 ② Message(메시지) : '나'가 아닌 '너'를 통해 지구촌 공감대 형성 ③ Channel(채널) : 유튜브, 페이스북, 인스타그램 등 소셜미디어를 활용해 지구촌 팬들과 실시간 접점 형성 ④ Receiver(수용자) : 한국과 아시아를 넘어 충성도 높은 글로벌 팬덤(A.R.M.Y.) 형성 ⑤ Effect(효과) : 방탄소년단의 경제적 부가가치 창출과 선한 영향력 확산

• 한국지형학회지
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• 제17권1호
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• pp.39-48
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• 2010

충남 온양천 유역의 후빙기 환경변화를 밝히기 위한 사례연구로서 온양천의 지류인 금곡천의 상류부(이하 조사지역)에 분포하는 선상지 퇴적층을 대상으로 화분분석을 실시하였다. 시료채취지점은 해발고도 약 67.5m로 현재 냉온대 중부 식생대에 속한다. 분석결과는 다음과 같다. 조사지역 일대는 약 3,000yrB.P. 이후 크게 SC-I기(期)(Pinus가 우점하는 침엽수림시대) → SC-II기(期)(Quercus·Castanea가 우점하는 낙엽활엽수림시대) → ③ SC-III기(期)(Pinus-Quercus-Ulmus/Zelkova가 우점하는 침활혼효림시대)로 변천했다. 각 시대의 연대는 SC-I기(期)와 SC-II기(期)가 약 3,000~2,000yrB.P., SC-III기(期)가 약 2,000yrB.P.~현재로 수렴된다. 전국적 후빙기 화분대와 대비하면, SC-I기(期)와 SC-II기(期)는 RIIIa 그리고 SC-III기(期)는 RIIIb에 대비된다. 그리고 약 2,000yrB.P. 이후 조사지역 일대는 삼림파괴에 따른 소나무림의 분포역이 확대되었으며, 또한 메밀화분의 출현율이 높은 점 등을 고려할 때, SC-III기(期)는 인간간섭의 시대로서 이 시대에 들어서면서부터 삼림파괴가 본격화되었고, 당시 거주민들은 농경생활을 했었을 것으로 추정된다.

• Animal Bioscience
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• 제36권4호
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• pp.555-569
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• 2023

Objective: The objective of this study was to investigate the effects of N⁶-Methyladenosine modification-circRNA-zinc finger protein 638 (m⁶A-circRNA-ZNF638) on the induced activation of secondary hair follicle (SHF) stem cells with its potential mechanisms in cashmere goats. Methods: The m⁶A modification of ZNF638 was analyzed using methylation immunoprecipitation with real-time quantitative polymerase chain reaction technique in SHF stem cells. The effects of circRNA-ZNF638 on the induced activation of SHF stem cells in m⁶A dependence were evaluated through the overexpression of circRNA-ZNF638/its m⁶A-deficient mutants in circRNA-ZNF638 knockdown SHF stem cells. The competitive binding of miR-361-5p to circRNA-ZNF638/Wnt5a 3'- untranslated region was analyzed through Dual-luciferase reporter assay. Results: The m⁶A-circRNA-ZNF638 had significantly higher transcription at anagen SHF bulge of cashmere goats compared with that at telogen, as well as it positively regulated the induced activation of SHF-stem cells in cashmere goats. Mechanismly, m⁶A-circRNA-ZNF638 sponged miR-361-5p to heighten the transcriptional expression of Wnt5a gene in SHF-stem cells. We further demonstrated that the internal m⁶A modification within circRNA-ZNF638 is required for mediating the miR-361-5p/Wnt5a pathway to regulate the induced activation of SHF stem cells through an introducing of m⁶A-deficient mutant of circRNA-ZNF638. Conclusion: The circRNA-ZNF638 contributes the proper induced activation of SHF-stem cells in cashmere goats in m⁶A-dependent manner through miR-361-5p/Wnt5a axis.

• 예술인문사회 융합 멀티미디어 논문지
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• 제8권7호
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• pp.485-496
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• 2018

본 논문에서는 스크린도어가 설치되어 있는 지하역사 승강장에서 화재가 발생시 승강장 환기실에 설치된 송풍기의 작동에 따른 연기거동을 실험적인 방법으로 측정하였다. 스크린도어가 닫힌 승강장에서 모의화재 발생시 승강장의 제연설비가 작동하지 않은 경우와 작동하는 경우에 제연 특성을 비교하였다. 화재연기를 모사하기 위하여 연기발생기에서 발생한 연기를 열풍기를 이용하여 가열하였다. 화재연기를 정량적으로 측정하기 위하여 연기농도계를 이용한 광투과도를 측정하였다. 스크린도어가 닫혀져있고 지하역사의 제연설비가 작동되지 않는 경우 승강장에 화재가 발생하면 연기가 승강장 내부에 축적되어 승객의 피난이 매우 어려우며, 매우 위험한 상황으로 연결될 수 있음을 확인할 수 있었다. 반면에 역사의 제연설비를 가동한 조건에서는 승강장 화재에 의해 발생한 연기가 역사의 제연설비를 통하여 외부로 빠져나가며, 대합실에서 승강장 방향으로 기류가 형성되어 승강장 승객이 대합실 방향으로 대피하는 것이 가능함을 확인할 수 있었다. 후속 논문에서는 승강장 인접부에 위치한 터널환기구를 통한 터널 제연의 병행효과에 대하여 다룰 예정이다.

• 디자인융복합연구
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• 제14권4호
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• pp.209-221
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• 2015

이 연구는 상품을 알리는 마케팅 측면에서 유용한 수단인 소셜미디어와 소비자를 설득하는 커뮤니케이션 측면에서 유용한 수단인 게이미피케이션 기법을 결합한 새로운 마케팅 트렌드를 심도 있게 살펴보고자 한다. 게이미피케이션은 오래전부터 다양한 분야에서 활용된 기법이나 고전적 게이미피케이션 기법과 소셜미디어 상에서의 게이미피케이션 기법에는 차이가 존재한다. 따라서 소셜미디어와 게이미피케이션에 대한 이론적 고찰을 바탕으로 소셜미디어와 게이미피케이션의 결합 현상의 배경과 특징을 살펴보았다. 그리고 게이미피케이션을 활용한 소셜미디어 마케팅 사례분석을 통해 사용자의 마케팅 참여 정도와 방법에 따라 일반참여형, 주도적 참여형, 창조행위형, 관계형, 체험형으로 기업의 마케팅을 유형화했다. 이를 통해 소셜미디어와 게이미피케이션이 결합할 때 나타나는 변화양상을 고전적 게임기법과 비교하였는데, 경쟁-성취-관계가 오늘날의 소비자에게 좀 더 유의미한 영향을 주는 요인으로 파악되었다. 이 연구는 게이미피케이션을 활용한 소셜미디어 마케팅의 변화된 게임기법을 연구하여 마케팅 전략에 도움이 되는 기반을 마련했다는 점에 의의가 있다.

• Journal of Microbiology and Biotechnology
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• 제33권6호
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• pp.831-839
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• 2023

Tylosin is a potent veterinary macrolide antibiotic produced by the fermentation of Streptomyces fradiae; however, it is necessary to modify S. fradiae strains to improve tylosin production. In this study, we established a high-throughput, 24-well plate screening method for identifying S. fradiae strains that produce increased yields of tylosin. Additionally, we constructed mutant libraries of S. fradiae via ultraviolet (UV) irradiation and/or sodium nitrite mutagenesis. A primary screening of the libraries in 24-well plates and UV spectrophotometry identified S. fradiae mutants producing increased yields of tylosin. Mutants with tylosin yield 10% higher than the wild-type strain were inoculated into shake flasks, and the tylosin concentrations produced were determined by high-performance liquid chromatography (HPLC). Joint (UV irradiation and sodium nitrite) mutagenesis resulted in higher yields of mutants with enhanced tylosin production. Finally, 10 mutants showing higher tylosin yield were re-screened in shake flasks. The yield of tylosin A by strains UN-C183 (6767.64 ± 82.43 ㎍/ml) and UN-C137 (6889.72 ± 70.25 ㎍/ml) was significantly higher than that of the wild-type strain (6617.99 ± 22.67 ㎍/ml). These mutant strains will form the basis for further strain breeding in tylosin production.

• Nuclear Engineering and Technology
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• 제55권5호
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• pp.1802-1813
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• 2023

Conjugate heat transfer between liquid metal and solid is a common phenomenon in a liquid-metal-cooled fast reactor's fuel assembly and heat exchanger, dramatically affecting the reactor's safety and economy. Therefore, comprehensively studying the sophisticated conjugate heat transfer in a liquid-metal-cooled fast reactor is profound. However, it has been evidenced that the traditional Simple Gradient Diffusion Hypothesis (SGDH), assuming a constant turbulent Prandtl number (Pr_t,, usually 0.85 - 1.0), is inappropriate in the Computational Fluid Dynamics (CFD) simulations of liquid metal. In recent decades, numerous studies have been performed on the four-equation model, which is expected to improve the precision of liquid metal's CFD simulations but has not been introduced into the conjugate heat transfer calculation between liquid metal and solid. Consequently, a four-equation model, consisting of the Abe k - ε turbulence model and the Manservisi k_𝜃 - ε_𝜃 heat transfer model, is applied to study the conjugate heat transfer concerning liquid metal in the present work. To verify the numerical validity of the four-equation model used in the conjugate heat transfer simulations, we reproduce Johnson's experiments of the liquid lead-bismuth-cooled turbulent pipe flow using the four-equation model and the traditional SGDH model. The simulation results obtained with different models are compared with the available experimental data, revealing that the relative errors of the local Nusselt number and mean heat transfer coefficient obtained with the four-equation model are considerably reduced compared with the SGDH model. Then, the thermal-hydraulic characteristics of liquid metal turbulent pipe flow obtained with the four-equation model are analyzed. Moreover, the impact of the turbulence model used in the four-equation model on overall simulation performance is investigated. At last, the effectiveness of the four-equation model in the CFD simulations of liquid sodium conjugate heat transfer is assessed. This paper mainly proves that it is feasible to use the four-equation model in the study of liquid metal conjugate heat transfer and provides a reference for the research of conjugate heat transfer in a liquid-metal-cooled fast reactor.