• Korean Journal of Social Welfare
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• v.40
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• pp.5-37
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• 2000

The purpose of this study is to find out some characteristics of the family functions of single mothers to provide some useful data for the development of programs which may help single mothers to improve their family functions. The McMaster Family Assessment Device (FAD) which is composed of problem solving function (PS), communication function (CF), role function (RC), emotional reaction function (ER), emotional involvement (EI), behavioral control function (BC), and general function (GC) and McCubbins' FSI were administered to single mothers and their adolescent children and mothers and their adolescent children of ordinary families (control family). The single mothers' perception of their family function was lower than that of control family mothers in an the 7 categories of FAD. The perceptions of the family function of the single mothers' children were lower than those of control family mothers' children. There was on significant difference between the perceptions of single mothers and their children in PS, ER, EI, GC of their family. However, single mothers' perception in RC and BC was lower than that of their children, and the children's perception in CF were lower than that of their mothers. Single mothers who fall on the period of single motherhood was shorter than 7 years perceived their families' function as lower than that of single mothers' who fall on the periods of single motherhood longer than 7 years in all categories. Generally speaking, the single mothers' family functions were lower than that of control family. The different characteristics of each single mother's family functions should be considered in developing programs for single mothers.

• Journal of the Korean Society of Industry Convergence
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• v.9 no.2
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• pp.117-122
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• 2006

This paper is to provide some of the analysis procedures of the FAD(Failure Assessment Diagram), which is applied th the safety test of the atomic pressure vessels and other structures. Harrison proposed R-6 Diagram, and Bloom developed the method of DPFAD(Deformation Plasticty Failure Assessment Diagram). Analysis of J-integration of the plastic materials give the failure test curve for the materials with serious work hardening effect. J value was obtained form both FEM(Finite Element Mothod) and the experiment using compact type specimen for J=JIC, and the results were compared. In this study, the results of analysis using DPFAD and the factors affecting DPFAD in Static fatigue and fracture were reviewed. It was shown that the concept of DPFAD can be applied to test the safety of the structures.

• Journal of Life Science
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• v.28 no.6
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• pp.656-662
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• 2018

Flavin-containing monooxygenases from Corynebacterium (cFMOs) were mutagenized based on homology modeling to develop variants with an enhanced indigoid production capability. The four mutants, F170Y, A210G, A210S, and T326S, which fused to a maltose-binding protein (MBP), were constructed, and their biochemical properties were characterized. Of these, purified MBP-T326S required a higher concentration of exogenous FAD (100 mM) than the wild-type MBP-cFMO for optimal activity and showed a 3.8-fold increase in the $k_{cat}/K_m$ value at $100{\mu}M$ FAD compared to that of MBP-cFMO at $2{\mu}M$ FAD. The indole oxygenase activities of MBP-T326S decreased to 63-77% compared to that of the MBP-cFMO In addition, MBP-T326S displayed a very low level of futile NADPH oxidase activities (21-24%) in the absence of a substrate. Mutant proteins except for T326S displayed similar $K_m$ and increased $k_{cat}/K_m$ values compared to the wild-type. MBP-F170Y and -A210S mutants showed elevated indole oxygenase activity higher than 3.1- and 2.9-fold, respectively, in comparison with MBP-cFMO. When indigoid production was carried out in LB broth with 2.5 g/l of tryptophan, Escherichia coli expressing cFMO produced 684 mg/l of indigo and 104 mg/l of indirubin, while cells harboring T326S produced 1,040 mg/l of indigo and 112 mg/l of indirubin. The results indicate that the production of indigo was 13% higher when compared to a previous report in which an E. coli expressing FMO from Methylophaga produced 920 mg/l of indigo. The protein engineering of cFMO based on homology modeling provided a more rational strategy for developing indigoid-producing strains.

• Korean Journal of Poultry Science
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• v.38 no.3
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• pp.181-189
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• 2011

A 5-month (May to November in 2009) monitoring program for five viral pathogens in litter, such as avian influenza virus A (AIV), infectious bronchitis virus (IBV), infectious bursal disease virus (IBDV), fowl adenovirus (FAdV), and chicken infectious anemia virus (CIAV) was conducted in 62 flocks at 31 broiler farms (two flocks in each farm) in Korea in 2009. Viral pathogens were examined twice (before and at the end of the rearing period) at 31 broiler farms, and included fresh litter (n = 16) and recycled litter (n = 15) farms. Thirty-seven viruses (14 IBVs, 2 IBDVs, 9 FAdVs, and 12 CIAVs) were isolated from 75% (12/16) and 73% (11/15) of fresh litter and reused litter farms during the period, respectively, indicating no difference in viral contamination rate between farms using new and reused litter. Of these isolates, three (two CIAVs and one IBDV) were isolated from recycled litter samples collected before the rearing period at three broiler farms, whereas the others (n=34) were isolated from fresh and recycled litter samples collected at the end of the rearing period. When the performances, involving viability, body weight, and feed conversion ratio, were compared, no significant differences were found between farms using fresh and recycled litter during the period.

• Transactions of the Korean hydrogen and new energy society
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• v.26 no.2
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• pp.179-183
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• 2015

In this study, we propose a catalyst structure including enzyme and metal nano rod for glucose sensing. In the catalyst structure, glucose oxidase (GOx) and gold nano rod (GNR) are alternatingly immobilized on the surface of carbon nanotube (CNT), while poly(ethyleneimine) (PEI) is inserted in between the GOx and GNR to fortify their bonding and give them opposite polarization ($[GOx/GNR]_nPEI/CNT$). To investigate the impact of $[GOx/GNR]_nPEI/CNT$ on glucose sensing, some electrochemical measurements are carried out. Initially, their optimal layer is determined by using cyclic voltammogram and as a result of that, it is proved that $[GOx/GNR/PEI]_2/CNT$ is the best layer. Its glucose sensitivity is $13.315{\mu}AmM^{-1}cm^{-2}$. When it comes to the redox reaction mechanism of flavin adenine dinucleotide (FAD) within $[GOx/GNR/PEI]_2/CNT$, (i) oxygen plays a mediator role in moving electrons and protons generated by glucose oxidation reaction to those for the reduction reaction of FAD and (ii) glucose does not affect the redox reaction of FAD. It is also recognized that the $[GOx/GNR/PEI]_3/CNT$ is limited to the surface reaction and the reaction is quasi-reversible.

• Applied Biological Chemistry
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• v.40 no.6
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• pp.501-507
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• 1997

Chloroplasts isolated from Stevia rebaudiana Bertoni leaves contained an enzyme activity which catalyzed hydroxylation of ent-kaurenoic acid (ent-kaur-16-en-19-oic acid; ent-KA) to steviol (ent-13-hydroxy kaur-16-en-19-oic acid), the diterpenoid carboxylic alcohol which is the aglycone of sweet stevioside-related glycosides. $[^(14)C]-methylated$ ent-KA was used to localize ent-KA hydroxylase. $[^(14)C]-methyl-KA$ was most actively was transformed into methyl-steviol in chloroplast. The enzymatic activity was found in stroma fraction but not in thylakoid membrane in Stevia rebaudiana Bertoni. However, ent-KA 13-hydroxylase activity was not detected in stroma fraction of either Spinacia oleracea and Solidago altissima. The reaction products using $[^(14)C]-methyl-KA$ were purified and identified on TLC autoradiogram. The hydroxylation of ent-KA from stromal protein to form steviol required NADPH and oxygen. FAD and riboflavin stimulated the enzyme activity 1.5-and 1.7-fold, respectively. It also turned out that the activity of this enzyme using methyl-KA as a substrate was 16.7% that of ent-KA. The purified ent-KA 13-hydroxylase did not act on t-cinnamic acid, 4-hydroxyphenyl acetic acid, choline and resorcinol, known as monooxygenase and hydroxylase substrates.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.270-273
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• 2001

The fluorescence sensor is utilized to monitor the complex fluorescence patterns of intra- and extracellular components in cultivation processes. Especially biogenic fluorophores such as proteins and peptides (tryptophan, phenylalanine), coenzymes (FAD, NAD(P)H) and vitamins (riboflavin, pyridoxine) within cells are detected by a fluorescence sensor. In this work a 2-dimensional fluorescence sensor has been used to monitor a production process of itaconic acid by Aspergillus terreus and the on-line monitored spectra data can be con-elated to off-line data measured by a few methods.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.586-586
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• 2005

• Journal of Korean Society for Quality Management
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• v.26 no.3
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• pp.1-16
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• 1998

Even though advocates of TQM emphasize the role of TQM more than before, the interest of the public at large have decreased in recent years. In order to explain the perceptual gap between the advocates and general public, three questions were considered: 1) Is TQM a dying fad\ulcorner 2) Does TQM focus on a particular facet of management\ulcorner 3) Does TQM pursue incremental improvement, while reengineering pursue breakthrough innovation\ulcorner It is also discussed how can we extend the horizon of TQM so as to integrate new management theories into the framework of TQM.