• Title/Summary/Keyword: fad

Search Result 224, Processing Time 0.025 seconds

Effect of Nutrient Limitation on Lipid Content and Fatty Acid Composition of Mutant Chlamydomonas reinhardtii (돌연변이 Chlamydomonas reinhardtii의 영양분 제한에 따른 지질 생산 및 지방산 조성 변화 연구)

  • Baek, Jaewon;Choi, Jong-il
    • KSBB Journal
    • /
    • v.30 no.2
    • /
    • pp.91-95
    • /
    • 2015
  • Production of biodiesel from microalgae is dependent on the microalgal lipid content and free fatty acid composition. Both lipid and free fatty acid are regulated by nutrient sources. In this study, newly developed mutant Chlamydomonas reinhardtii with higher lipid content was investigated for the effect of nutrient limitation. Nitrogen $NO_3{^{-}}$ and phosphate $PO_4{^{3-}}$ were limited for nutrient starvation during the cultivation. Under nutrient starvation, total lipid content level was increased to 27~33% and C16:0 fatty acid content constituted over 31~43% of total fatty acid. Interestingly, we also found that the expression of fatty acid desaturase (FAD7) was decreased when nutrients were starved.

Network Optimization in the Inhomogeneous Distribution Using Genetic Algorithm Traffic (유전자 알고리즘을 이용한 비균일 트래픽 환경에서의 셀 최적화 알고리즘)

  • 박병성;한진규;최용석;조민경;박한규
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.27 no.2B
    • /
    • pp.137-144
    • /
    • 2002
  • In this paper, we optimize the base station placement and transmission power using genetic approach. A new representation describing base station placement and transmit power with real number is proposed, and new genetic operators are introduced. This new representation can describe the locations, powers, and number of base stations, Considering coverage, power and economy efficiency, we also suggest a weighted objective function. Our algorithm is applied to an obvious optimization problem, and then it is verified. Moreover, our approach is tried in inhomogeneous traffic distribution. Simulation result proves that the algorithm enables to fad near optimal solution according to the weighted objective function.

A Study on the Family Functioning in the Family with the Cancer Patient (암환자가족의 가족기능에 대한 조사연구)

  • Han, Suk-Jung
    • Asian Oncology Nursing
    • /
    • v.3 no.2
    • /
    • pp.155-163
    • /
    • 2003
  • This study was designed to assess the level of family function between cancer patient's families and normal families, and to seek better quality family-Mcentered nursing care. The subjects for this study were 95 ca patient's families who were selected from th university hospitals and 95 normal families who selected from 1 university hospital. The instrument used for this study was McMaster Family Assessment Device (FAD) developed by Epstein, Baldwin and Bishop (1983). The scale consisted of 53 items covering 7 areas: 'problem solving', 'communication', 'roles', 'affective responsiveness', 'affective involvement', 'behavior control', and 'general functioning'. As for data analyses, descriptive statistics, ${\chi}^2-test$, t-test, ANOVA were adopted using SAS program. The result of this study was as follows: There there was no significant difference between cancer patient's family function and normal family function. Also there was no significant difference on cancer patient's family function with regard to family characteristics, disease's characteristics, and clinical stage of cancer.

  • PDF

Presenilin-2 mutation perturbs ryanodine receptor-mediated calcium homeostasis, caspase-3 activation and increases vulnerability of PC12 cells

  • Hwang, In-Young;Shin, Im-Chul;Hwang, Dae-Youn;Kim, Young-Kyu;Yang, Ki-Hwa;Ha, Tae-Yeol;Hong, Jin-Tae
    • Proceedings of the Korean Society of Toxicology Conference
    • /
    • 2003.05a
    • /
    • pp.73-74
    • /
    • 2003
  • Familial form of Alzheimer's disease (FAD) is caused by mutations in presenilin-1 and presenilin-2 (PS2). PS1 and PS2 mutation are known to similar effects on the production of amyloid $\beta$ peptide (A$\beta$) and cause of cell death in the Alzheimer's brain. The importance of the alternation of calcium homeostasis in the neuronal cell death by PS1 mutation in a variety of experimental system has been demonstrated. (omitted)

  • PDF

A Comprehensive Review on r-Learning: Authentic r-Learning Beyond the Fad of New Educational Technology

  • Jung, Sung Eun;Han, Jeonghye
    • International journal of advanced smart convergence
    • /
    • v.9 no.2
    • /
    • pp.28-37
    • /
    • 2020
  • We conducted a comprehensive review on the previous research on r-Learning. By reviewing 843 previous studies about r-Learning published from 2004 to 2015, this study investigated 1) the trend of research on r-Learning over time, 2) the characteristics of targeted students in r-Learning, 3) the educational activities implemented for r-Learning, and 4) the types of educational robots used for r-Learning. The study found that the research on r-Learning has rapidly and steadily increased and the types of educational activities and educational robots has been diversified. Relying on the findings of this review, this study suggests 1) ensuring growth in both the quality and the quantity of research on r-Learning, 2) broadening the target student population of r-Learning beyond the age-limited boundaries, 3) enhancing educational activities of r-Learning, and 4) recognizing the necessity for systematic and clear concepts of types of educational robots.

Flavobacterium meningosepticum이 생산하는 Nucleoside Oxidase의 효소학적 특성

  • 최양문;조홍연;양한철
    • Microbiology and Biotechnology Letters
    • /
    • v.24 no.5
    • /
    • pp.579-584
    • /
    • 1996
  • The molecular weight of the purified nucleoside oxidase estimated by gel filtration column chromatography was 480,000 and the enzyme protein was composed of four nonidentical subunits (81,000, 69,000, 32,000 and 16,000). On the basis of the visible absorption spectra and the enzymatic determination of the purified enzyme, the enzyme was supposed as a hemoprotein and also a flavoprotein containing 3 moles of FAD per I mole of enzyme. The isoelectric point of the enzyme was pH 5.1. Addition of metal salts such as 1 mM SnCl$_{2}$ and PbCl$_{2}$ into an enzyme reaction solution inhibited the enzyme activity by 94 and 90%, respectively. The enzyme activity was also lost significantly by hemoenzyme inhibitors such as NaCN and NaN$_{3}$ and flavoenzyme inhibitor, acriflavine and quinacrine. The maximal nucleoside oxidase activity was observed at pH 7.0 and 55$\circ$C. The nucleoside oxidase was relatively stable in the range of pH 5.5-9.0 and below 55$\circ$C.

  • PDF

Nonlinear Analysis of Seismic Responses of Large Tilting fad Journal Bearings (대형 틸팅패드 저어널 베어링의 비선형 지진응답 해석)

  • Kim Sung-Gi;Kim Kyung-Woong
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
    • /
    • 2003.11a
    • /
    • pp.281-288
    • /
    • 2003
  • In this paper, seismic responses of large tilting pad journal bearings which have $3\~5$ tilting pads were numerically analyzed. The turbulent lubrication equation, the energy equation and motion equation were solved at each time step. The regime of operation of this bearing is laminar, turbulent and transitional. Also viscosity of working fluid was considered as function of only temperature and inlet pressure build-up was considered. Numerical results for a large tilting pad journal bearing showed journal center, maximum temperature, maximum pressure, friction torque. The relationship of bearing response and seismic intensity are discussed.

  • PDF

Enzymatic Properties of Intracellular Adenosine Deaminase from Nocardioides sp. J-326TK

  • Hong-Ki Jun;Tae-Sook Kim
    • Journal of Life Science
    • /
    • v.9 no.1
    • /
    • pp.64-68
    • /
    • 1999
  • The properties of purified intracellular adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) of Nocardioides sp. J-326TK isolated from soil have been studied. The enzyme deaminated adenosine and 2`-deoxyadenosine and the respective {TEX}$K_{M}${/TEX} values were 4.0×{TEX}$10^{-4}${/TEX} M and 5.0× {TEX}$10^{-4}${/TEX} M, but the enzyme was not active on 8-bromoadenosine, 6-methylaminopurine riboside, ATP, ADP, 2`-AMP, 3`-AMP, 5`-AMP, dAMP, cAMP, NAD, FAD, NADP and adenine. The enzyme activity was strongly inhibited by the addition of {TEX}$Hg^{2+}${/TEX} and {TEX}$Ag^{+}${/TEX}, {TEX}$Cu^{2+}${/TEX}, {TEX}$Co^{2+}${/TEX} and {TEX}$Mn^{2+}${/TEX} also inhibited the activity but much less extent. The effect of alkyl reagents, metal chelating reagents and certain other compounds on the enzyme activity were also examined. No reagent activated the enzyme. On the contrary, the enzyme reaction was slightly inhibited by o-phenanthroline and 6-benzyladenosine.

  • PDF

A Study on the 60 GHz Band Radial Line Slot Array Antenna Fed by a Rectangular Waveguide (60 GHz 대역용 도파관 급전 Radial Line Slot Array 안테나에 관한 연구)

  • 김용훈;채희덕;이중원;박종국;김성철;김성철
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
    • /
    • v.13 no.3
    • /
    • pp.257-262
    • /
    • 2002
  • A single-layered radial line slot array (SL-RLSA) antenna etched on a substrate and fad by a rectangular waveguide is presented in the 60 ㎓ band. The design curves are obtained by an efficient electromagnetic coupling analysis using Ewald Sum technique and Shanks transformation. The antenna has rectangular waveguide feed structure using a rectangular waveguide-to-radial line transition. The prototype antenna of 10 cm-diameter was tested and the gain of 31 ㏈i and the efficiency of 38% were measured at 60 ㎓.

The Studies of Copolymer, Poly (3-hydroxybutyrate-co-3-hydroxy valerate) Synthesis in Recombinant pha+ Escherichia coli

  • Lee, Ha-Young;Rhie, Ho-Gun
    • Proceedings of the Zoological Society Korea Conference
    • /
    • 1996.10a
    • /
    • pp.195.2-195
    • /
    • 1996
  • acs gene cloning was constructed by subcloning the 2.2-kb MunI-MunI restriction fragment of 638 and 639 which include acs gene from the kohara phage into the unique EcoRI site of pUC18 and pJM9131 containing the PHA biosynthesis genes. Then recombinant E. coli fadRatoC(Con) mutants containing the polyhydroxyalkanoate(PHA) biosynthesis genes are able to incoporate s significant levels of 3-hydroxyvalerate (3HV) into the copolymer [P(3HB-co-3HV)]. Quantitative determination of PHB and P(3HB-co-3HV) was performed by gas-chromatographic analysis of extracts obtained from methanolysis of lyophilized cells.

  • PDF