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http://dx.doi.org/10.7841/ksbbj.2015.30.2.91

Effect of Nutrient Limitation on Lipid Content and Fatty Acid Composition of Mutant Chlamydomonas reinhardtii  

Baek, Jaewon (Department of Biotechnology and Bioengineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University)
Choi, Jong-il (Department of Biotechnology and Bioengineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University)
Publication Information
KSBB Journal / v.30, no.2, 2015 , pp. 91-95 More about this Journal
Abstract
Production of biodiesel from microalgae is dependent on the microalgal lipid content and free fatty acid composition. Both lipid and free fatty acid are regulated by nutrient sources. In this study, newly developed mutant Chlamydomonas reinhardtii with higher lipid content was investigated for the effect of nutrient limitation. Nitrogen $NO_3{^{-}}$ and phosphate $PO_4{^{3-}}$ were limited for nutrient starvation during the cultivation. Under nutrient starvation, total lipid content level was increased to 27~33% and C16:0 fatty acid content constituted over 31~43% of total fatty acid. Interestingly, we also found that the expression of fatty acid desaturase (FAD7) was decreased when nutrients were starved.
Keywords
Chlamydomonas reinhardtii; Fatty acid; Lipid;
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