Proceedings of the Zoological Society Korea Conference (한국동물학회:학술대회논문집)
- 1996.10a
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- Pages.195.2-195
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- 1996
The Studies of Copolymer, Poly (3-hydroxybutyrate-co-3-hydroxy valerate) Synthesis in Recombinant pha+ Escherichia coli
- Lee, Ha-Young (Department of Biology, Kyunghee University) ;
- Rhie, Ho-Gun (Department of Biology, Kyunghee University)
- Published : 1996.10.01
Abstract
acs gene cloning was constructed by subcloning the 2.2-kb MunI-MunI restriction fragment of 638 and 639 which include acs gene from the kohara phage into the unique EcoRI site of pUC18 and pJM9131 containing the PHA biosynthesis genes. Then recombinant E. coli fadRatoC(Con) mutants containing the polyhydroxyalkanoate(PHA) biosynthesis genes are able to incoporate s significant levels of 3-hydroxyvalerate (3HV) into the copolymer [P(3HB-co-3HV)]. Quantitative determination of PHB and P(3HB-co-3HV) was performed by gas-chromatographic analysis of extracts obtained from methanolysis of lyophilized cells.
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