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DRAG EFFECT OF KOMPSAT-1 DURING STRONG SOLAR AND GEOMAGNETIC ACTIVITY (강한 태양 및 지자기 활동 기간 중에 아리랑 위성 1호(KOMPSAT-1)의 궤도 변화)

  • Park, J.;Moon, Y.J.;Kim, K.H.;Cho, K.S.;Kim, H.D.;Kim, Y.H.;Park, Y.D.;Yi, Y.
    • Journal of Astronomy and Space Sciences
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    • v.24 no.2
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    • pp.125-134
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    • 2007
  • In this paper, we analyze the orbital variation of the Korea Multi-Purpose SATellite-1(KOMPSAT-1) in a strong space environment due to satellite drag by solar and geomagnetic activities. The satellite drag usually occurs slowly, but becomes serious satellite drag when the space environment suddenly changes via strong solar activity like a big flare eruption or coronal mass ejections(CMEs). Especially, KOMPSAT-1 as a low earth orbit satellite has a distinct increase of the drag acceleration by the variations of atmospheric friction. We consider factors of solar activity to have serious effects on the satellite drag from two points of view. One is an effect of high energy radiation when the flare occurs in the Sun. This radiation heats and expands the upper atmosphere of the Earth as the number of neutral particles is suddenly increased. The other is an effect of Joule and precipitating particle heating caused by current of plasma and precipitation of particles during geomagnetic storms by CMEs. It also affects the density of neutral particles by heating the upper atmo-sphere. We investigate the satellite drag acceleration associated with the two factors for five events selected based on solar and geomagnetic data from 2001 to 2002. The major results can be summarized as follows. First, the drag acceleration started to increase with solar EUV radiation with the best cross-correlation (r = 0.92) for 1 day delayed F10.7. Second, the drag acceleration and Dst index have similar patterns when the geomagnetic storm is dominant and the drag acceleration abruptly increases during the strong geomagnetic storm. Third, the background variation of the drag accelerations is governed by the solar radiation, while their short term (less than a day) variations is governed by geomagnetic storms.

Identification of Quantitative Trait Loci Associated with Anthracnose Resistance in Chili Pepper (Capsicum spp.) (고추 탄저병 저항성 관련 양적형질 유전자좌 분석)

  • Kim, Su;Kim, Ki-Taek;Kim, Dong-Hwi;Yang, Eun-Young;Cho, Myeong-Cheoul;Jamal, Arshad;Chae, Young;Pae, Do-Ham;Oh, Dae-Geun;Hwang, Ju-Kwang
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.1014-1024
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    • 2010
  • Pepper ($Capsicum$ spp.) anthracnose caused by $Colletotrichum$ $acutatum$ is a destructive disease susceptible to areas where chili peppers are grown. $Capsicum$ $baccatum$ var. $pendulum$ (Cbp) is resistant to anthracnose and has actively been used for interspecific hybridization for the introgression of resistance gene(s) into cultivated chili peppers. The goals of this study were to determine the inheritance of resistance to anthracnose within $Capsicum$ $baccatum$ and to map quantitative trait loci (QTLs) for the anthracnose resistance. A genetic mapping population consisting of 126 $F_2$ plants derived from a cross between $Capsicum$ $baccatum$ var. $pendulum$ (resistant) and $Capsicum$ $baccatum$ 'Golden-aji' (susceptible) was used for linkage mapping. The linkage map was constructed with 52 SSRs, 175 AFLPs, and 100 SRAPs covering 1,896cM, with an average interval marker distance of 4.0cM. Based on this map, the number, location, and effect of QTLs for anthracnose resistance were studied using plants inoculated in the laboratory and field. A total of 19 quantitative trait loci (2 major QTLs and 16 minor QTLs) were detected. Two QTLs ($An8.1$, $An9.1$) showed 16.4% phenotypic variations for anthracnose resistance after wounding inoculation. In addition, five minor QTL loci ($An7.3$, $An7.4$, $An4.1$, $An3.1$, $An3.2$) showed a total of 60.73% phenotypic variations of anthracnose resistance in the field test. Several significant QTLs were also detected and their reproducibility was confirmed under different inoculation conditions. These QTLs are now being confirmed with different breeding populations. Markers tightly linked to the QTLs that are reliable under different environmental conditions will help to determine the success of marker-assisted selection for anthracnose -resistant breeding programs in chili pepper.

Investigating The Potential of Human Hair Produced from The Beauty Parlor and Barbershop as a Raw Material of Wood Adhesives (미·이용업 폐기물 인모의 목재접착제 원료화 가능성 탐색)

  • Yang, In;Ahn, Sye Hee
    • Journal of the Korean Wood Science and Technology
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    • v.45 no.5
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    • pp.599-612
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    • 2017
  • Human hair (HH) is produced as a waste from beauty parlor and barbershop. HH-based adhesives were formulated with NaOH-hydrolyzed HH, $H_2SO_4$-hydrolyzed chicken blood (CB) and PF as a crosslinking agent. Physicochemical properties and retention rate against hot water of the adhesives were measured to investigate the potential of HH as a raw material of wood adhesives. HH was composed of keratin-type protein of 80% and over. Ash of less than 0.1% was contained in HH. Among the amino acids included in HH, glutamic acid showed the highest content, followed by cysteine, serine, arginine and threonine. Solid content of the adhesives ranged from 33.2% to 41.8% depending on hydrolysis conditions of HH and PF type. Viscosity at $25^{\circ}C$ ranged from 300 to $600mPa{\cdot}s$ resulting in a sprayable adhesive. Retention rate against hot water measured to evaluate the water resistance of adhesives was the highest in the cured resin formulated with 5% NaOH-hydrolyzed HH and 5% $H_2SO_4$-hydrolyzed CB. Meanwhile, the molar ratio of formaldehyde to phenol in PF did not have a significant impact on the retention rate of HH-based adhesives. When the retention rates of HH-based adhesives were compared to those of conventional wood adhesive resins used for the production of wood-based panels extensively, HH-based adhesives formulated with 30 wt% PF showed lower retention rate than commercial urea-formaldehyde resin. However, when PF content was increased to 35 wt%, the retention rate greatly increased and approached to that of commercial melamine-urea-formaldehyde resin. Except for the results mentioned above, the analysis of economic feasibility suggests that HH-based adhesives can be used for the production of wood-based panels if HH is hydrolyzed in proper conditions and then the HH-based adhesives are formulated by the HH hydrolyzates with 35 wt% PF.

Characteristics of Groundwater Quality in Sasang Industrial Area, Busan Metropolitan City (부산시 사상공단지역의 지하수 수질 특성)

  • Hamm, Se-Yeong;Kim, Kwang-Sung;Lee, Jeong-Hwan;Cheong, Jae-Yeol;Sung, Ig-Hwan;Jang, Seong
    • Economic and Environmental Geology
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    • v.39 no.6 s.181
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    • pp.753-770
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    • 2006
  • In urban areas, groundwater pollution is heavily affected by urbanization with land use types. This study aims to characterize groundwater quality and contamination in Sasang industrial area of Busan Metropolitan City where metalworking, machinery and footwear factories are located. Busan Metropolitan City is the highest in the utilization of groundwater resources among the metropolitan cities in Korea. $K^+,\;Na^+,\;Ca^{2+},\;Mg^{2+},\;Cl^-,\;SO_4^{2-}\;and\;HCO_3^-$ concentrations, and electrical conductivity (EC), total dissolved solids (TDS) and salinity are high in the areas near the Nakdong River. The results are attributed to the influence of salt water which intruded into the coastal sediments during sedimentation. In addition, the dominant chemical type of Ca-Cl indicates the influence of salt water in the geological formations as well as anthropogenic pollution. $SiO_2$ ion is interpreted to originate from both water-silicate mineral reactions and the decomposition of cement concretes. Trichloroethylene (TCE) was detected at 12 sites of total 18 sites. However, tetrachloroethylene (PCE) was detected at f sites and 1.1.1-trichloroethane (TCA) at 3 sites. According to the factor analysis, factor 1 was explained by 49.8%, factor 2 19.8%, and factor 3 11.0% with total 80.6% explanation. pH, TDS, salinity, $Ca^{2+},\;K^+,\;Mg^{2+},\;Na^+,\;Al^{3+},\;As^{3+},\;Cl^-\;and\;Fe^{2+}$ were positively highly loaded to factor 1. The chemical components loaded to factor 1 represent the chemical characteristics of both industrial pollution and influence by salt water. Based on the cluster analysis and distribution pattern of chemical components, the concentration of $Na^+,\;Ca^{2+},\;Cl^-,\;SO_4^{2-}\;K^+,\;and\;Mg^{2+}$ is high in the riverside area of the Nakdong River composed of coastal sediments that is influenced by salt water. The downstream area of the Hakjang Stream is judged to be affected by both salt water and artificial pollution. The other part of the study area is interpreted by anthropogenic pollution.

A study on some high school boys' practice of oral health control(around toothbrushing) and knowledge of decay and preferred taste (일부 남고생의 간식 선호맛과 구강관리실천(잇솔질 중심) 및 우식지식도 조사)

  • Choi, Yun-Hwa
    • Journal of Korean society of Dental Hygiene
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    • v.10 no.2
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    • pp.361-374
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    • 2010
  • Objectives : The purpose of this study was to provide some basic data for effective prevention and oral health education for oral health. For that purpose, a self-administered questionnaire was distributed to the boys of three high schools in Gyeonggi Province. Methods : The questionnaire consisted of items about the practice of oral health control and knowledge of decay according to grade, number of snacking, preferred taste, and toothbrushing. Total 487 questionnaires were put to analysis using the SPSS WIN 13.0 program. Results : 1. The biggest number of the boys brushed their teeth twice a day. The 10th graders brushed teeth three times a day, and the 11th and 12th graders tended to brush teeth twice a day(${\chi}^2$=18.21, p<.05). 2. As for the way of toothbrushing, 39.0% of the boys said they brushed teeth the way they felt like, being followed by those who said they brushed teeth turning the toothbrush up and down and brushing both the teeth and gums(32.6%). As for the number of snacking, those who had snacks three times a day or more brushed teeth turning the toothbrush up and down and brushing both the teeth and gums. Those who hardly had snacks more tended to brush teeth the way they felt like than those who had snacks(${\chi}^2$=21.28, p<.05). And as for preferred tastes, those who preferred a sweet and salty taste more tended to brush teeth the way they felt like than others. And those who preferred a hot taste brushed teeth turning the toothbrush up and down and brushing both the teeth and gums(${\chi}^2$=26.85, p<.01). 3. The respondents' percentage of correct answers for 'Decay is caused by bacteria' was 81.5%. Those who preferred a hot taste recorded the highest percentage of correct answers(${\chi}^2$=21.95, p<.00). 4. Their percentage of correct answers for 'Decay is accompanied by pain from the beginning' was 78.2%. The 12th graders had the highest rate of correct answers, and the 11th graders had the highest rate of wrong answers(${\chi}^2$=11.56, p<.00). 5. Their percentage of correct answers for 'Fluorine is helpful to the prevention of decay' was 75.4%. Those who hardly had snacks recorded the highest rate of correct answers(${\chi}^2$=10.05, p<.02). 6. Their percentage of correct answers for 'There is a way to supply fluorine into the waterworks' was 29.2%, which means the percentage of wrong answers was much higher. The percentage of wrong answers was the highest in the 11th grade(${\chi}^2$=7.06, p<.03). 7. The respondents' mean scores of knowledge of decay were 13.15 of total 18. While the 12th graders showed the highest level of knowledge of decay, the 11th graders were lower in the level of knowledge of decay than the other graders. And there were significant differences(F=5.41, p<.01) according to grade with no such differences according to the number of snacking and preferred taste. Conclusions : Oral health education should be applied to the boys all through their life to have a great effect, and it is important that they should always put the oral health education into practice to be a successful education.

Effect of Seeding Dates on the Growth Characteristics, Yield and Feed Value of Whole Crop Azuki bean in Jeju Island (제주지역에서 파종기에 따른 청예팥의 생육반응.수량 및 사료가치 변화)

  • Cho, Nam-Ki;Kang, Young-Kil;Song, Chang-Ki;Kang, Yong-Chul;Cho, Young-Il;Go, Mi-Ra
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.23 no.1
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    • pp.7-12
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    • 2003
  • This study was conducted to determine the influence of seeding date (May 3, 13, 23, June 2, 12) on the growth characteristics yield and feed value Azuki bean(Vigna angularis W.F. Wight) from May 3, 2002 to August 3, 2002 in Jeju province. Plant height was the longest (84cm) when seeded on 13 May and was the shortest (48.4cm) when seeded on 12 June. Numbers of branches and leaves per plant, stem diameter and weight of leaves and stems per plant were the same trend with plant height. Fresh forage, fry matter, crude protein and TDN(total digestible nutrient) yield per ha at were increased to 54.6MT, 7.7MT, 1.4MT and 4.5MT, respectively, whereas decreased as seeding date was delayed and then at seeding on 12 June were decreased to 11.5MT/ha 2.12MT/ha 0.4MT/ha and 1.3MT/ha, respectively The content of crude protein, crude fat, NFE(nitrogen free extract) and TDN were incresed 17.6%∼21.3%, 3.3%∼4.5%, 34.3%∼41.4% and 56%∼64.8%, respectively, as seeding was delayed from 3 May to 13 June. Whereas the content of crude fiber and crede ash were decreased 33.8%∼23.5% and 11%∼9.3%, respectively.

Bioequivalence of SCD Zaltoprofen Tablet to Soleton® Tablet (Zaltoprofen 80 mg) (솔레톤 정(잘토프로펜 80 mg)에 대한 삼천당잘토프로펜 정의 생물학적동등성)

  • Kang, Hyun-Ah;Park, Sun-Ae;Kim, Dong-Ho;Kim, Hwan-Ho;Yun, Hwa;Kim, Kyng-Ran;Yoo, Hee-Doo;Park, Eun-Ja;Cho, Hye-Young;Lee, Yong-Bok
    • Journal of Pharmaceutical Investigation
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    • v.36 no.3
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    • pp.209-215
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    • 2006
  • Zaltoprofen, (2-(10,11-dihydro-10-oxodibenzo[b,f]thiepin-2-yl)propionic acid) is an NSAID with powerful anti-inflammatory effects as well as an analgesic action on inflammatory pain. The purpose of the present study was to evaluate the bioequivalence of two zaltoprofen tablets, $Soleton^{\circledR}$ (CJ Corp.) and SCD Zaltoprofen (Samchundang Pharmaceutical Co., Ltd.), according to the guidelines of the Korea Food and Drug Administration (KFDA). The release of zaltoprofen from the two zatoprofen formulations in vitro was tested using KP Vlll Apparatus ll method with various dissolution media. Twenty six healthy male subjects, $23.2{\pm}2.26$ years in age and$64.7{\pm}8.08$ kg in body weight, were divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After a single tablet containing 80 mg as zaltoprofen was orally administered, blood samples were taken at predetermined time intervals and the concentrations of zaltoprofen in serum were determined using HPLC with UV detector. The dissolution profiles of two formulations were similar in all tested dissolution media. The pharmacokinetic parameters such as $AUC_t$, $C_{max}$ and $T_{max}$ were calculated, and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed $AUC_t$, $C_{max}$ and untransformed $T_{max}$. The results showed that the differences between two formulations based on the reference drug, $Soleton^{\circledR}$ were 6.33, 5.91 and 17.7% for $AUC_t$, $C_{max}$ and untransformed $T_{max}$, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log 0.8 to log 1.25 (e.g.,log $1.01{\sim}1og\;1.11$ and log $0.928{\sim}1og\;1.18$ for $AUC_t$ and $C_{max}$, respectively). Thus, the criteria of the KFDA bioequivalence guideline were satisfied, indicating SCD Zaltoprofen tablet was bioequivalent to $Soleton^{\circledR}$ tablet.

Role of Catecholamines in Ventricular Fibrillation (Catecholamines에 관(關)하여 -제4편(第四編) : 심실전동발생(心室顫動發生)에 있어서의 catecholamines의 의의(意義)-)

  • Lee, Woo-Choo
    • The Korean Journal of Pharmacology
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    • v.19 no.1
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    • pp.15-35
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    • 1983
  • Although it has been well known that ventricular fibrillation is the most important complication during hypothermia, much investigation has failed to show the exact nature of the etiology of ventricular fibrillation. Recently, there has been considerable research on the relationship between sympathetic activity and ventricular fibrillation under hypothermia. Cardiac muscle normally contains a certain amount of norepinephrine and the dramatic effect of this catecholamines on the cardiac muscle is well documented. It is, therefore, conceivable that cardiac catecholamines might exert an influence on the susceptibility of heart muscle to tachycardia, ventricular fibrillation and arrhythmia, under hypothermia. Hypothermia itself is stress enough to increase tonus of sympatheticoadrenal system. The normal heart is supplied by an autonomic innervation and is subjected to action of circulating catecholamines which may be released from the heart. If the reaction of the heart associated with a variable amount of cardiac catecholamines is. permitted to occur in the induction of hypothermia, the action of this agent on the heart has not to be differentiated from the direct effects of cooling. The studies presented in this paper were designed to provide further information about the cardio-physiological effects of reduced body temperature, with special reference to the role of catecholamines in ventricular fibrillation. Healthy cats, weighing about 3 kg, were anesthetized with pentobarbital(30 mg/kg) intraperitoneally. The trachea was intubated and the endotracheal tube was connected to a C.F. Palmer type A.C. respirator. Hypothermia was induced by immersing the cat into a ice water tub and the rate of body temperature lowering was $1^{\circ}C$ per 5 to 8 min. Esophageal temperature and ECG (Lead II) were simultaneously monitored. In some cases the blood pH and serum sodium and potassium were estimated before the experiment. After the experiment the animals were killed and the hearts were excised. The catecholamines content of the cardiac muscle was measured by the method of Shore and Olin (1958). The results obtained are summarized as follows. 1) In control animal the heart rate was slowed as the temperature fell and the average pulse rates of eight animals were read 94/min at $31^{\circ}C$, 70/min at $27^{\circ}C$ and 43/min at $23^{\circ}C$ if esophageal temperature. Ventricular fibrillation was occurred with no exception at a mean temperature of $20.3^{\circ}C(21-l9^{\circ}C)$. The electrocardiogram revealed abnormal P waves in each progressive cooling of the heart. there was, ultimately, a marked delay in the P-R interval, QRS complex and Q-T interval. Inversion of the T waves was characteristic of all animals. The catecholamines content of the heart muscle excised immediately after the occurrence of ventricular fibrillation was about thirty percent lower than that of the pre-hypothermic heart, that is, $1.0\;{\mu}g/g$ wet weight compared to the prehypothermic value of $1.41\;{\mu}g/g$ wet weight. The changes of blood pH, serum sodium and potassium concentration were not remarkable. 2) By the adrenergic receptor blocking agent, DCI(2-3 mg/kg), given intramuscularly thirty minutes before hypothermia, ventricular fibrillation did not occur in one of five animals when their body temperature was reduced even to $16^{\circ}C$. These animals succumbed at that low temperature, and the changes of heart rate and loss of myocardial catecholamines after hypothermia were similar to those of normal animals. The actual effect of DCI preventing the ventricular fibrillation is not predictable. 3) Administration of reserpine(1 mg/kg, i.m.) 24 hours Prior to hypothermia disclosed reduced incidence of ventricular fibrillation, that is, six of the nine animals went into fibrillation at an average temperature of $19.6^{\circ}C$. By reserpine myocardial catecholamines content dropped to $0.045\;{\mu}g/g$ wet weight. 4) Bretylium pretreatment(20 mg/kg, i.m.), which blocks the release of catecholamines, Prevented the ventricular fibrillation under hypothermia in four of the eight cats. The pulse rate, however, was approximately the same as control and in some cases was rather slower. 5) Six cats treated with norepinephrine(2 mg/kg, i.m.) or DOPA(50 mg/kg) and tranylcypromine(10 mg/kg), which tab teen proved to cause significant increase in the catecholamines content of the heart muscle, showed ventricular fibrillation in all animals under hypothermia at average temperature of $21.6^{\circ}C$ and the pulse rate increased remarkably as compared with that of normal. Catecholamines content of cardiac muscle of these animals markedly decreased after hypothermia but higher than control animals. 6) The functional refractory periods of isolated rabbit atria, determined by the paired stimulus technique, was markedly shortened by administration of epinephrine, norepinephrine and isoproterenol. 7) Adrenergic beta-blocking agents, such as pronethalol, propranolol and sotalol(MJ-1999), inhibited completely the shortening of refractory period induced by norepinephrine. 8) Pretreatment with either phenoxftenbamine or phentolamine, an adrenergic alphatlocking agent, did not modify the decrease in refractory period induced by norepinephrine. From the above experiment it is possible to conclude that catecholamines play an important role in producing ventricular fibrillation under hypothermia. The shortening of the refractorf period of cardiac muscle induced by catecholamines mar be considered as a partial factor in producing ventriculr fibrillaton and to be mediated by beta-adrenergic receptor.

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Studies on the Quantitative Changes of Riboflavin during Takju Brewing (탁주양조중 Riboflavin의 소장에 관한 연구)

  • Kim, Chan-Jo;Choi, Woo-Young
    • Applied Biological Chemistry
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    • v.13 no.3
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    • pp.219-222
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    • 1970
  • Riboflavin in the various materials which used for Takju brewing was determined and its quantitative changes in the Takju mashes which were mashed with polished rice and wheat flour respectively using Nuruk only for enzyme source, and with wheat flour and corn powder respectively using Nuruk and Koji, were checked at 24 hours intervals during the whole brewing period. The results obtained were as follows. a) The riboflavin contents in 100 grams of each materials were: Polished rice $46.9{\gamma}$ Wheat flour $67.3{\gamma}$ Corn powder $73.9{\gamma}$ Nuruk $169.0{\gamma}$ Koji of wheat flour $87.1{\gamma}$ Koji of corn powder $84.4{\gamma}$ b) About 30 to 40 percent of riboflavin in the polished rice, wheat flour and corn powder were losed by steaming at 10 pounds for 50 minutes. c) Riboflavin content in the plots used Nuruk only was decreased during 3 days after mashing and then increased markedly after 5 days and, more or less, decreased after 6 days. Quantitative changes of riboflavin in the plots used Nuruk and Koji showed a rugged curve without decreasing in early stages, and its content was also increased markedly 5 days after mashing and then decreased a little after 6 days. d) Approximately $82{\gamma}$ of riboflavin was determined in 100ml. of the ripened mash of the rice plot and $72{\gamma}$ in the wheat flour plot in the case of using Nuruk only. $88.6{\gamma}$ of riboflavin were determined in 100ml. of the ripened mash of the wheat flour plot and $83.6{\gamma}$ in the corn powder plot in the case of using Nuruk and Koji. e) It was assumed that $28.8{\gamma}$ to $41{\gamma}$ of riboflavin were contained in 100 ml. of finished Takju of the two plots used Nuruk only and 33.6 to 44.5${\gamma}$ in the both two plots used Nuruk and Koji. f) About $47.3{\gamma}$ of riboflavin were determined in 100 grams of Takju cake of the rice plot and $66.2{\gamma}$ in the wheat flour plot used Nuruk only, and $62.9{\gamma}$ in the corn powder plot and $64.5{\gamma}$ in the wheat flour plot used Nuruk and Koji.

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Studies on the Yeasts for the Brewing of Soy Sauce(2) -Isolation, identification and classification of the yeasts in the soy sauce mash- (간장발효에 관여하는 효모에 관한 연구 (제2보) -간장 덧 중에 생육하는 효모에 대하여-)

  • Lee, Taik-Soo;Lee, Suk-Kun;Shin, Bo-Kyu
    • Applied Biological Chemistry
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    • v.13 no.2
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    • pp.171-180
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    • 1970
  • The yeasts in the soy sauce mash were isolated and identified, and they were classified by coloring with the treatment of TTC(2, 3, 5, triphenyltetrazolium chloride) agar and counted in process of time. The results obtained were as follows: a) The number of ordinary and osmophilic yeasts in 1 ml. of the soy sauce mash showed a tendency to be increased from the mashing to the mature stages and to decrease in the aging stages: $127{\times}10^3$ immediately after mashing, $83{\times}10^3$ 1 month after, $356{\times}10^3$ 3 months after, $1250{\times}10^3$ 6 months after and $65{\times}10^3$ 2 years after mashing in the case of ordinary yeasts, and 0 after mashing, $40{\times}10^3$ 1 month after, $81{\times}10^3$ 3 months after, $358{\times}10^3$ 6 month after and $23{\times}10^3$ 2 years after mashing in the case of osmophilic yeasts. b) 50 strains of yeasts were isolated from the soy sauce mash optionally in process of fermentation period, and they were identified as 7 genera and 18 species: 10 strains of Saccharomyces rouxii, 1 strain of Saccharomyces marxianus, 3 strains of Saccharomyces rosei, 1 strain of Saccharomyces fermentati, 6 strains of Saccharomyces mellis, 1 strain of Saccharomyces acidifaciens, 1 strain of Saccharomyces pastori, 3 strains of Pichia polymorpha, 2 strains of Hansenula anomala, 1 strain of Hansenula saturnus, 2 strains of Hansenula suaveolens, 5 strains of Nadsonia fulvescens, 8 strains of Debaryomyces hasenii, 1 strain of Debaryomyces nicotianae, 1 strain of Debaryomyces kloeckeri, 2 strains of Torulopsis sake, 1 strain of Torulopsis holmii and 1 strain of Candida pelliculasa. c) Distribution of yeasts according to the fermentation period was as follows: i) Saccharomyces rouxii, Saccharomyces marxianus, Saccharoymces rosei, Pichia polymorpha, Debaryomyces hansenii, Torulopsis sake, Candida pelliculosa, Debaryomyces nicotianae, Nadsonia fulvescens, Hansenula suaveolens and Hansenula saturnus were found in the early stages of fermentation. ii) Saccharomyces rouxii, Saccharomyces rosei, Saccharomyces fermentati, Saccharomyces mellis, Saccharomyces pastori, Hansenula anomala, Saccharomyces acidifaciens and Debaryomyces hansenii appeared in the mature stages. iii) Saccharomyces rouxii, Saccharomyces mellis, Nadsonia fulvescenes, Dedaryomyces hansenii, Debaryomyces kloeckeri, Torulopsis sake and Torulopsis holmii were distributed in the aging stages. d) TTC white yeasts were found in abundance in the early stages of fermentation and TTC red yeasts appeared more than 50 per cent in the mature and aging stages. e) The yeasts belonging to Saccharomyces mellis and Saccharomyces pastori were classified as TTC red yeasts, Saccharomyces acidifaciens were reel pink, Hansenula saturnus Debaryomyces kloeckeri, and Torulopsis holmii were pink, Saccharomyces marxianus and Nadsonia fulvescens were white and the others were the same as the description in the previous report. Saccharomyces rouxii ware classified for the most part as TTC red yeasts, and while some of them were red pink. f) Species of yeasts in the soy sauce mash were similar to those in the soy sauce koji, but the latter were not osmophilic and in the former case, the osmophilic yeasts were increased in process of fermentation period.

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