Browse > Article

Identification of Quantitative Trait Loci Associated with Anthracnose Resistance in Chili Pepper (Capsicum spp.)  

Kim, Su (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science)
Kim, Ki-Taek (The Foundation of Agri. Technology Commercialization and Transfer)
Kim, Dong-Hwi (Horticultural & Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science)
Yang, Eun-Young (Vegetable Research Division, National Institute of Horticultural & Herbal Science)
Cho, Myeong-Cheoul (Vegetable Research Division, National Institute of Horticultural & Herbal Science)
Jamal, Arshad (College of Science & Arts Rabaigh Campus, King Abdullaziz University)
Chae, Young (Technology Services Division, National Institute of Horticultural & Herbal Science)
Pae, Do-Ham (The Foundation of Agri. Technology Commercialization and Transfer)
Oh, Dae-Geun (Korea National College of Agriculutre and Fisheries)
Hwang, Ju-Kwang (Department of Horticultural Science, Chungbuk National University)
Publication Information
Horticultural Science & Technology / v.28, no.6, 2010 , pp. 1014-1024 More about this Journal
Abstract
Pepper ($Capsicum$ spp.) anthracnose caused by $Colletotrichum$ $acutatum$ is a destructive disease susceptible to areas where chili peppers are grown. $Capsicum$ $baccatum$ var. $pendulum$ (Cbp) is resistant to anthracnose and has actively been used for interspecific hybridization for the introgression of resistance gene(s) into cultivated chili peppers. The goals of this study were to determine the inheritance of resistance to anthracnose within $Capsicum$ $baccatum$ and to map quantitative trait loci (QTLs) for the anthracnose resistance. A genetic mapping population consisting of 126 $F_2$ plants derived from a cross between $Capsicum$ $baccatum$ var. $pendulum$ (resistant) and $Capsicum$ $baccatum$ 'Golden-aji' (susceptible) was used for linkage mapping. The linkage map was constructed with 52 SSRs, 175 AFLPs, and 100 SRAPs covering 1,896cM, with an average interval marker distance of 4.0cM. Based on this map, the number, location, and effect of QTLs for anthracnose resistance were studied using plants inoculated in the laboratory and field. A total of 19 quantitative trait loci (2 major QTLs and 16 minor QTLs) were detected. Two QTLs ($An8.1$, $An9.1$) showed 16.4% phenotypic variations for anthracnose resistance after wounding inoculation. In addition, five minor QTL loci ($An7.3$, $An7.4$, $An4.1$, $An3.1$, $An3.2$) showed a total of 60.73% phenotypic variations of anthracnose resistance in the field test. Several significant QTLs were also detected and their reproducibility was confirmed under different inoculation conditions. These QTLs are now being confirmed with different breeding populations. Markers tightly linked to the QTLs that are reliable under different environmental conditions will help to determine the success of marker-assisted selection for anthracnose -resistant breeding programs in chili pepper.
Keywords
AFLP; Colletotrichum acutatum; linkage map; SRAP;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Park, H.K., B.S. Kim, and W.S. Lee. 1990b. Inheritance of resistance to anthracnose (Colletotrichum spp.) in pepper (Capsicum annuum L.) II. Genetic analysis of resistance to Colletotrichum dematium. J. Kor. Soc. Hort. Sci. 31:207-212.
2 Shin, H.J. 1997. Screening for resistance anthracnose (Colletotrichum gloeosporioides) and its inheritance in pepper (Capsicum annuum L.). M.S. thesis Seoul Natl. Univ. Seoul, Korea.
3 Stuber, C.W., M.D. Edwards, and J.F. Wendel. 1987. Molecular marker-facilitated investigation of quantitative trait loci in maize. II: Factors influencing yield and its component traits. Crop Sci. 27:639-648.   DOI
4 Li, G. and C.F. Quiros, 2001. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica Theor. Appl. Genet. 103:455-461.   DOI   ScienceOn
5 Ohm, H.W. and G.E. Shaner. 1976. Three components of slow-leaf rusting at different growth stages in wheat. Phytopathology 66:1356-1360.   DOI
6 Pae, D.H., W.M. Lee, and J.Y. Yoon. 1995. Breeding for resistance in chili pepper. NHRI Annual Report. p. 19-28.
7 Parlevliet, J.E. 1975. Components of resistance that reduce the rate of epidemic development. Annu. Rev. Phytopathol. 17: 203-222.
8 Kim, S.H., J.B. Yoon, J.W. Do, and H.G. Park. 2008a. Resistance to anthracnose caused by Colletotrichum acutatum in chili pepper. J. Crop Sci. Biotech. 10:277-280.
9 Landder, E.S. and D. Bostein. 1986. Strategies for studying heterogeneous genetic traits in humans by using a linkage map of restriction fragment length polymorphisms. Proc. Natl. Acad. Sci. USA 83:7353-7357.   DOI   ScienceOn
10 Kim, S.H., J.B. Yoon, and H.G. Park. 2008b. Inheritance of anthracnose resistance in a new genetic resource, Capsicum baccatum PI594137. J. Crop Sci. Biotech. 11:13-16.
11 Hadden, J.F. and L.L. Black. 1989. Anthracnose of Pepper Caused by Colletotrichum spp. Proceeding of the International Symposium on Integrated Management Practices: Tomato and Pepper Production in the Tropics; Taiwan: Asian Vegetable Research and Development Centre. p. 189-199.
12 Caldwell, R.M. 1968. Breeding for general and/or specific plant disease resistance. In: Finlay, K.W. and K.W. Shepherd. Proceedings of Third International Wheat Genetics Symposium. Australian Academy of Science, Canberra, p. 263-272.
13 Johnson, R. 1988. Durable resistance to yellow (stripe) rust in wheat and its implications in plant breeding. In: Simmonds, N.W. and S. Rajaram (eds). Breeding strategies for resistance to the rusts of wheat. CIMMYT, Mexico D.F., Mexico, p. 63-75.
14 Kim, J.T., S. Park, W. Choi, Y. Lee, and H.T. Kim. 2008. Characterization of Colletotrichum isolates causing anthracnose of pepper in Korea. Plant Pathol. J. 24:17-23   과학기술학회마을   DOI
15 Do, J.W. 2005. QTL analysis for anthracnose resistance in an introgressed $BC_{1}F_{2}$ population from the Capsicum annuum ${\times}$ Capsicum baccatum. M.S. Thesis, Seoul Natl. Univ., Seoul, Korea.
16 Dolye, J.J. and J.L. Dolye. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13-15.
17 AVRDC. 1999. Off-season tomato, pepper and eggplant. p. 20-30 in: AVRDC 1998. Progress Report. Taiwan.
18 Berke, T., L.L. Black, and C.A. Liu. 1999. Breeding for anthracnose and Phytophthora resistance in hot pepper (Capsicum annuum). J. Kor. Capsicum Res. Coop. 5:1-15.
19 Chae, Y. 2006. Study on the characteristics of interspecific hybrids and their progenies for the breeding of anthracnose-resistant pepper. Ph.D. thesis, Seoul Univ., Seoul, Korea.
20 Yoon, J.B. and H.G. Park. 2001. Screening method for resistance to pepper fruit anthracnose: Pathogen sporulation, inoculation methods related to inoculum concentrations, and postinoculation environments. J. Kor. Soc. Hort. Sci. 42:389-393.
21 Voorrips, R.E., R. Finkers, L. Sanjaya, and R. Groenwold. 2004. QTL mapping of anthracnose (Colletotrichum spp.) resistance in a cross between Capsicum annuum and C. chinense. Theor. Appl. Genet. 109:1275-1282.   DOI   ScienceOn
22 Yoon, J.B. 2003. Identification of genetic resources, interspecific hybridization and inheritance analysis for breeding pepper (Capsicum annuum) resistant to anthracnose. Ph.D. Thesis, Seoul Natl. Univ., Seoul, Korea.
23 Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper, and M. Zabeau. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23:4407-4414.   DOI   ScienceOn
24 Yang, D.C. 2006, Interspecific introgression breeding and identification of QTLs for anthracnose resistance in Capsicum spp. Ph.D. Thesis, Seoul Natl. Univ., Seoul, Korea.
25 Yi, G.B., J.M. Lee, S.H. Lee, D.I. Choi, and B.D. Kim. 2006. Exploitation of pepper EST-SSRs and an SSR-based linkage map. Theor. Appl. Genet. 114:113-130.   DOI   ScienceOn
26 Van der Plank, J.E. 1963. Plant diseases; epidemics and control. Academic Press, New York.
27 Van Ooijen, J.W. and R.E. Voorrips. 2001. JOINMAP 3.0, software for the calculation of genetic linkage maps. Plant Research International, Wageningen.
28 Voorrips, R.E. 2002. Mapchart: software for the graphical presentation of linkage maps and QTLs. J. Hered. 93:77-78.   DOI   ScienceOn
29 Park, H.K., B.S. Kim, and W.S. Lee. 1990a. Inheritance of resistance to anthracnose (Colletotrichum spp.) in pepper (Capsicum annuum L.) I. Genetic analysis of anthracnose resistance by diallel crosses. J. Kor. Soc. Hort. Sci. 31:91-105.