• Clinical and Experimental Reproductive Medicine
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• 제29권2호
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• pp.149-157
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• 2002

Objectives: To assess the scavenging effect of carnitine derivatives on oxidative damage to sperm during sperm processing, cryopreservation and thawing. Materials and Methods: Fresh semen samples from 20 normal healthy volunteers were collected by masturbation after at least 48 hours abstinence. After liquefaction of semen samples at room temperature, the specimens were diluted with sperm wash media (Ham's F-10, Life technologics) to a uniform density of $20{\times}10^6/ml$. L-carnitine or acetylcarnitine were added with various concentration of $0{\mu}M$, $10{\mu}M$, $30{\mu}M$ in semen sample or cryoprotectant. All specimens were cryopreservated at $-196^{circ}C$ $LN_2$ for 3 days. Sperm motility, vitality, fertilizing capacity, reactive oxygen species formation and the level of lipid peroxidation were analyzed by computer assisted semen analyzer, eosin-nigrosin stain, hypoosmotic swelling test, chemiluminescence and thiobarbituric acid method, respectively, during sperm processing, cryopreservation and thawing. Results: The sperm motility was only increased in proportion to the concentration of acetylcarnitine with no statistical significance (p>0.05). The sperm vitality was also significantly improved in proportion to the concentration of acetylcarnitine with statistical significance (p<0.05). The sperm fertilizing capacity was significantly increased in proportion to the concentration of L-carnitine and acetylcarnitine and reactive oxygen species generation and lipid peroxidation were significantly decreased with same fashion (p<0.05). On comparison of effects between L-carnitine and acetylcarnitine, acetylcarnitine was superior to L-carnitine on the improvement of sperm motility and vitality as well as the suppression of reactive oxygen species generation and lipid peroxidation. Conclusions: These results suggest that carnitine derivatives have a scavenging effect against oxidative damages during sperm processing, cryopreservation and thawing. Therefore, carnitine derivatives may be useful as an oral antioxidant in patients with male infertility due to increased ROS generation.

• Journal of Ginseng Research
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• 제40권2호
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• pp.141-150
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• 2016

Background: Adipocyte-macrophage communication plays a critical role regulating white adipose tissue (WAT) inflammatory gene expression. Because WAT inflammation contributes to the development of metabolic diseases, there is significant interest in understanding how exogenous compounds regulate the adipocyte-macrophage crosstalk. An aqueous (AQ) extract of North American (NA) ginseng (Panax quinquefolius) was previously shown to have strong inflammo-regulatory properties in adipocytes. This study examined whether different ginseng extracts influence adipocyte-macrophage crosstalk, as well as WAT inflammatory gene expression. Methods: The effects of AQ and ethanol (EtOH) ginseng extracts ($5{\mu}g/mL$) on adipocyte and macrophage inflammatory gene expression were studied in 3T3-L1 and RAW264.7 cells, respectively, using real-time reverse transcription polymerase chain reaction. Adipose tissue organ culture was also used to examine the effects of ginseng extracts on epididymal WAT (EWAT) and inguinal subcutaneous WAT (SWAT) inflammatory gene expression. Results: The AQ extract caused significant increases in the expression of common inflammatory genes (e.g., Mcp1, Ccl5, Tnf-${\alpha}$, Nos2) in both cell types. Culturing adipocytes in media from macrophages treated with the AQ extract, and vice versa, also induced inflammatory gene expression. Adipocyte Ppar-${\gamma}$ expression was reduced with the AQ extract. The AQ extract strongly induced inflammatory gene expression in EWAT, but not in SWAT. The EtOH extract had no effect on inflammatory gene expression in either both cell types or WAT. Conclusion: These findings provide important new insights into the inflammo-regulatory role of NA ginseng in WAT.

• 한국작물학회지
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• 제29권3호
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• pp.232-241
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• 1984

수도 약배양의 효율을 높이기 위하여 callus 유기와 녹색체 출현율에 영향하는 화분의 발육단계, 화분의 저온처리와 기관 및 배지의 영향에 대한 일련의 실험을 수행하였으며 이에서 얻어진 결과를 요약하면 다음과 같다. 1. 단핵기의 화분에서 callus 유기율이 높았으며 이때는 대체로 엽신간장이 5~8cm에 해당되나 품종에 따라 차이가 있다. 2. 이삭을 상, 중, 하로 나누어 조사한 결과 엽이간장이 5~8cm의 것은 상부와 중간부분에 단핵기에 해당되는 것으로 나타났고 9~10cm 정도의 것은 중부와 하부 부분이 단핵기에 해당하는 것으로 나타났다. 3. 일반적으로 저온처리는 8$^{\circ}C$에서 12$^{\circ}C$가 callus 유기에 알맞는 것으로 나타났다. 그러나 온도에 대한 반응은 품종에 따라 차이가 있어 4$^{\circ}C$에서 callus 유기가 잘된 것도 있었다. 4. 저온 처리기간은 일반적으로 8~12일 정도에서 callus 유기가 잘 되었으나 품종간 차이가 있다. 5. 본 실험에서는 N6D 배지보다 N6 배지에서 callus 유기율이 높았다. 6. 대체로 녹색체 출현율은 4$^{\circ}C$와 8$^{\circ}C$로 처리한 것이 12$^{\circ}C$ 처리보다 높았다. 그러나 품종간 차이가 있었다. 7. 녹색체 출현에 좋은 결과를 주었던 온도 처리기간은 대체로 8~12일로 나타났다. 8. 본 실험에서 녹색체 출현율은 N6 배지보다 개량 MS 배지에서 2배 정도 높았다.

• 한국양식학회지
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• 제12권1호
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• pp.7-14
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• 1999

갈래곰보의 원혈질체 분리와 배양 조건을 확립하여 생명공학기술에 의한 유전적 형질개량의 기초조건을 마련코자 하였다. Abalone acetone powder,소라의 내장조효소, Pseudomanas와 Vibrio의 조효소, Cellulas R-10, Macerozyme R-10, Hemicellulase, Pectinase, Driselase, Protease 등을 0.6M mannitol과 0.5% potassium dextran sulfate를 함유한 50mM MES 해수완충액(pH 6.0)에 단독 또는 조합하여 조제한 효소액을 갈래곰보의엽체에 처리하였을 때 Abalone acetone powder 4.0% + Macerozyme R-10 4.0% + Hemicellulase 4.0% 효소조합액의 처리로서 생체조직 1g당 $107.6{\times}10^4$개의 원혈질체를 분리시킬 수 있었다. 갓 분리한 원형질체는 투명한 타원형으로 $7{\mu} m$~ $24{\mu} m$의 범위에 분포하였다. 0.2M mannitol을 첨가한 $ASP_{12}$배지에 배양한 세포는 9일 후 분열되고 25일 후 발아하였다. $ASP_{12}$배지는 f/2배지보다 갈래 곰보의 원혈질체 배양에 효과적이었으며 Guillard 항생물질조합액의 처리는 분화에 장애가 되었다.

• Wind and Structures
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• 제2권3호
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• pp.133-150
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• 1999

Significant costs to the public and private sectors due to recent extreme wind events have motivated the need for systematic post-hurricane damage data collection and analysis. Current post disaster data are collected by many different interested groups such as government agencies, voluntary disaster relief agencies, representatives of media companies, academicians and companies in the private sector. Each group has an interest in a particular type of data. However, members of each group collect data using different techniques. This disparity in data is not conducive to quantifying damage data and, therefore, inhibits the statistical and spatial description of damage and comparisons of damage among different extreme wind events. The data collection does not allow comparisons of data or results of analyses within a group and also prohibits comparison of damage data and information among different groups. Typically, analyses of data from a given event lead to different conclusion depending upon the definition of damage used by individual investigators and the type of data collected making it difficult for members of groups to compare the results of their analyses with a common language and basis. A formal method of data collection and analysis-within any single group-would allow comparisons to be made among different individuals, hazardous events and eventually among different groups, thus facilitating the management and reduction of damage due to future disaster. This research introduces a definition of damage to single family dwellings, and a common method of data collection and analysis suited for groups interested in regional characterization of damage. The current state-of-data is presented and a method for data collection is recommended based on these existing data collection methods. A fixed-scale damage index is proposed to consider the damage to a dwelling's feature. Finally, the damage index is applied to three dwellings damaged by Hurricane Iniki (1992). The damage index reflects the reduced functionality of a structure as a single family detached dwelling and provides a means to evaluate regional damage due to a single event or to compare damage due to events of different severity. Evaluation of the damage index and the data available support recommendation for future data collection efforts.

• 한국식생활문화학회지
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• 제28권4호
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• pp.339-347
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• 2013

Yaksun, a medicinal diet, has been traditionally prepared and applied (based on theories in oriental medicine) for the modulation of disease symptoms and signs. However, restaurants that serve and claim Yaksun mainly focus on stamina foods. A consistent definition of Yaksun has not been provided, which can confuse the public interpretation of Yaksun. The purpose of this study was to investigate the perception of Yaksun in Korean adults living in Seoul, Gyeonggi, and Chungbuk regions. Among the participants (M=55, F=168, 25y), only 10.4% understood the definition of Yaksun (mainly through the broadcast media). The frequency of Yaksun consumed when eating out was 2~3 times per month in 50.2% of participants. The main reason for choosing a Yaksun menu (46.3% of participants) when eating out was for health. The mean satisfaction score of Yaksun was $3.5{\pm}0.8$ on the five point Likert scale. Participants highly agreed ($3.8{\pm}0.8$) that Yaksun is composed of nutritious foods combined with oriental medicinal herbs for the treatment of disease, which was significantly higher in groups with learning experience on Yaksun (p<0.05). Interestingly, participants showed neutral to the description, that a diet without oriental medicinal herbs is not Yaksun ($3.1{\pm}1.0$), which was significantly different between genders (p<0.05). Men recognized more than women that Yaksun should be based on oriental medical theory (p<0.05) and should be prepared for the prevention or treatment of diseases (p<0.05). In conclusion, the concept and terminology of Yaksun need to be defined and publicized in modern diet.

• Journal of Preventive Medicine and Public Health
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• 제51권6호
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• pp.320-325
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• 2018

Objectives: This pilot study tested the effectiveness of a brief alcohol-related intervention delivered by the social media app WeChat to teach about ethanol-induced facial flushing and increase the willingness of students who see another student flushing to suggest that he or she should reduce or stop drinking. In the context of Chinese drinking culture, it is sometimes socially difficult to refuse a drink, even when experiencing physical discomfort, such as flushing. Methods: Classrooms of students in a medical university in China were randomly assigned to the intervention or control group. Students in the intervention group were invited to view 3 alcohol education lessons on WeChat during a 2-week period. A pretest and posttest before and after the 2-week period assessed changes in students' willingness to intervene if they saw someone flush while drinking. Data were collected about students' alcohol use and their ratings of the lessons. Results: Mixed-design analysis of variance yielded a significant time-by-treatment interaction effect on the variable of willingness to suggest that a flushing person stop or slow down their drinking, and the change was significant between the intervention and control groups. One-way analysis of covariance yielded a significant treatment effect at the posttest, after controlling for the pretest score. Students rated the lessons above the midpoint of the scale for being informative, interesting, and useful. Conclusions: The pilot study showed that a brief alcohol-related intervention delivered by WeChat could produce a measurable positive change in the willingness of university students to suggest that a student who flushes should stop drinking. This pilot study also suggested improvements for future lessons and evaluation design.

• 방송공학회논문지
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• 제28권2호
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• pp.194-212
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• 2023

다수의 색상 및 거리 순서쌍으로 구성된 몰입형 영상 압축을 위한 MPEG immersive video (MIV) 표준은 시점 간 중복 영역 제거 후 잔여 영상을 병합하여 높은 압축률을 확보하였다. 비슷한 영역을 표현하는 시점 간 그룹화를 통해 품질 향상 및 선택적 스트리밍 구현이 가능하나, 최근 그룹 기반 MIV 부호화 기술은 활발히 논의되고 있지 않다. 본 논문은 최신 MIV 참조 소프트웨어에서 그룹 기반 부호화 기술을 이식하고, 최적의 그룹 별 시점 및 영상 개수 산출을 위한 실험을 진행하였으며, 출력 영상 내 잔여 영상의 비율을 기반으로 전역적 영상 표현을 위한 최적의 출력 영상 수를 결정하는 기법을 제안한다.

• 한국가정과교육학회지
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• 제33권2호
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• pp.81-93
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• 2021

본 연구의 목적은 고등학생의 한복 이미지, 한복 선호 유형, 전통한복 인식을 알아보고 성별 및 한복수업 참여 여부에 따른 차이를 확인하는 것이다. 이를 위해 부산 지역 고등학생 438명의 자료를 수집한 후 SPSS를 사용하여 빈도 분석, 교차 분석, t-검정, F-검정으로 분석하였다. 본 연구 결과는 다음과 같다. 첫째, 대부분의 고등학생은 한복을 우아한 이미지로 인식하고 있었으나 대중매체 및 온라인상에서 화려한 한복 디자인에 노출되어 한복에 대한 화려한 이미지도 가지고 있는 것으로 나타났다. 한복 이미지는 성별, 한복수업 참여 여부 및 성별을 고려한 집단에서 통계적 유의미성이 확인되었다. 촌스러운 이미지는 남학생이 여학생에 비해 높은 비율을 보였고, 한복수업 미참여 남학생 집단이 수업 참여 남학생 집단에 비해 3배 이상 높은 비율로 나타났다. 둘째, 고등학생이 선호하고 있는 한복 유형은 전통한복이었으며 그 다음으로는 퓨전한복, 생활한복, 패션한복 순으로 나타났다. 한복 선호 유형은 성별, 한복수업 참여 여부 및 성별을 고려한 집단에 따라 차이를 보였다. 특히 패션한복에 대한 선호는 한복수업참여 집단이 미참여 집단에 비해 높은 비율로 나타났다. 셋째, 고등학생의 전통한복에 대한 인식도는 보통 이상이었고 하위요소별로 살펴보면 심미성, 지속가능성, 실용성 순으로 나타났다. 전통한복 인식은 한복수업 참여 여부, 성별, 한복수업 참여 여부 및 성별을 고려한 집단에서 유의미한 차이를 보였다. 여학생이 남학생에 비해 그리고 한복수업 참여 학생이 미참여 학생에 비해 전통한복을 긍정적으로 평가한 것으로 나타났다.

• 한국버섯학회지
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• 제21권4호
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• pp.241-246
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• 2023

본 연구는 농촌진흥청에서 육성한 백승과 국외 수집자원(KMCC02260)을 모본으로 하여 단핵균주를 분리 후 mon-mon교잡을 통해 '백운' 품종을 육성하였다. 균사배양 특성은 25℃에서 두 품종의 균사생장이 가장 좋았으며 15, 25℃에서 백운의 균사생장 속도가 대조품종보다 더 빨랐으며 배지별 시험에서도 모든 배지에서 백운의 균사생장이 우수하였다. 톱밥 병에 접종 후 균사 배양기간은 백운이 2일, 초발이일수도 1일 더 빨라 전체적인 생육기간을 단축시켰으며 수량은 228.0±10.9 g/병(850 ml)으로 대조품종 대비 8.3% 증수하였다. 백운의 형태적인 특성은 갓이 크고 대가 굵으며 갓이 순백색이었다. 대치배양과 유전적 다형성 검정 결과, 백운이 두 모본과 대조품종과 유전적으로 다르다는 것을 확인하였다.