• Korean Journal of Agricultural Science
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• 제9권2호
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• pp.461-466
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• 1982

Lower and upper petiole segments of strawberry plants (Fragaria${\times}$ananassa) were cultured on Murashige and Skoog (MS) medium containing 2mg/liter Benzylamino purine (BA), 0.02mg/liter 2,4-dichlorophenoxyacetic acid (2,4-D), or their coinbination. Of the lower petiole segments, 55% or more were differentiated on the media containing $2mg/{\ell}\;BA$ or $2mg/{\ell}\;BA+0.02mg/{\ell}\;2,4-D$. There was no significant difference in results be tween these treatments. BA did not influence root formation. No shoots we re initiated from the upper petiole segments. Root formation was stimulated by the $0.02mg/{\ell}\;2,4-D$ medium, whereas it inhibited shoot formation. Upper petiole segments produced more roots than did lower petiole segments.

• Microbiology and Biotechnology Letters
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• 제29권2호
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• pp.72-77
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• 2001

Expression of f3 ~agarase Gene and Catabolite Repression in Escherichia coli by the Promoter of Alginate Lyase Gene Isolated from Marine Pseudomonas sp. Jin, Cheal~Ho, J~Hyeon Park, Jeong-Hyun Han, YoonM Hyeok Chae, Jong~Hee Lee, Jung-Kee Lee!, and In-800 Kong*. Faculty of Food Science and Biotechnology, Pukyong National UniversitYt Pusan 608-737, Korea, llnBioNet Co. 1690-3 Taejon 306-230, Korea - Promoter is a key factor for expression of the recombinant protein. There are many promoters for overexpression of protein in various organisms. The aly promoter of Pseudomonas sp. W7 isolated from marine environment was known to be a constitutive expression promoter of the alginate lyase gene, and it's promoter activity is repressed by glucose in Escherichia coli. To investigate the catabolite repression of the aly promoter ~md association between the promoter mutants, f3 agarase gene, which was also cloned from Pseudomonas sp. W7 was connected to the aly promoter with the sequence the coding 46 N-terminal amino acids ofthe alginate lyase gene. The constructed plasmid was introduced into E. coli and the agarase activity was measured. Fourty six amino acids of the alginate lyase gene was serially deleted using peR to the direction of 5' upstream region and subcloned. The agarase was overexpressed by the aly promoter and the production of agarase was repressed by the addition of glucose into culture media. Fourty six amino acids of alginate lyase did not affect the production of agarase at all. The deletion of a putative stem-loop structure in the aly promoter induced the decrease of f3 -agarase productivity.

• The Journal of the Korea Contents Association
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• 제14권7호
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• pp.45-57
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• 2014

This study examines subjects' eye movement pattern and surveys their attitudes to the exposed advertisements on the Facebook. Different from the F-shaped pattern of the typical Web pages, users' eye movements on the Facebook have shown a rough H-shape. Even though a large number of users have shown F-shaped pattern on the ordinary Web pages in order to skip the contents of a Web, subjects' eye-movement pattern on the Facebook has H -shaped pattern due to the unique User Interface (UI) of the Facebook. With the right side and vertical arrangement of ads on the Facebook, users skip the page with having a large H-shaped pattern. In addition, this study set four AOIs(Area of Interest) that are advertising sections comprised on the Facebook Web page and measured fixation length within the AOIs then surveyed subjects' attitudes about the exposed ads. Through the experiment and survey, this study offers the optimum advertising position that can attract Facebook users' attention. As the result of experiment and survey, the second ad has the subjects' highest attitude to advertising and fourth ad is the next effectiveness and first and third ad followed. This study highlights the key implications to provide better user experiences(UX) and marketing strategies to users who are the consumers of companies and organizations which have a plan to put their advertising on the Facebook.

• Microbiology and Biotechnology Letters
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• 제34권3호
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• pp.228-234
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• 2006

The culture conditions were investigated to maximize the production of laccase from Fomitella fraxinea mycelia. Among the tested media, mushroom complete medium (MCM) showed the highest production of the enzyme. The optimum culture medium was 2% dextrose, 0.4% $(NH_4)_{2}HPO_4$, 0.05% $Na_{2}HPO_{4}{\cdot}7H_{2}O$, and 0.05% KCl as carbon, nitrogen, phosphorus, and inorganic salt sources respectively. SDS-PAGE followed by laccase activity staining using 2,6-djmethoxyphenol as the substrate was performed to identify the laccase activity under culture conditions studied. Zymogram analysis of the culture supernatant showed a laccase band with a molecular mass of 50 kDa. The enzyme production from F. fraxinea was reached to the highest level after the cultivation for 10 days at $25^{\circ}C$ and initial pH 8. The enzyme activity of the culture supernatant was most active at $50^{\circ}C$ and pH 5.

• Journal of Korea Multimedia Society
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• 제19권12호
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• pp.1919-1926
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• 2016

The people who complain of pain due to voice causes such as vocal cord nodules is increasing year by year. If the voice is changed, it is possible to give to colleagues discomfort or inconvenience during conversation. In this paper, we propose a way to reduce discomfort by improving the husky voice during the conversation. A VBF (voice boosting filter) is firstly designed to improve the husky voices. This filter may further emphasize the formant frequency components than the frequency components around the formant frequency, because the value is relatively greater than the other frequency. And a fixed-point type DSP chipset, TMS320F2812 is applied to the system, the operating frequency is 150MHz. The system was implemented as a compact for use as a portable, its size is $2.5cm{\times}10cm$. Through the test using three husky voices with some type of statement, it was satisfactory in processing speed and sound quality improvement.

• Journal of the Korean Institute of Landscape Architecture
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• 제28권6호
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• pp.127-132
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• 2001

The purpose of this research is to compare and analyze scientific names and Korean names of interior plants, considering that consumes' purchasing systems consist of internet and communication media in the era of informationalization, to help the consumers purchasing plants, and to propose some directions for the unification of Korean name in practical circulation. This survey was performed during 4 months from May to September in 2000. The survey places for research were wholesale flower market of YangJje Dong and Nam Seoul. The used plants for research were 37 family, 76 genus and 120 species. The main methods of data collection were interview and reference f plants picture books. The main reference for identifying plant name was accomplished by Yoon Ypung-Sub's HORTUS KOREANA. The research results are as follows; 1) Fifty one plants were identified as using generic or specific name. 2) Thirteen plants were identified as using varietal name. 3) Twenty four plants were identified as using common name recoded in a plant dictionary. 4)Thirteen plants were identified as using incorrect name. Consequently, it was identified that the research for the unification of Korean name of interior landscape plants should be performed thorough continuous cooperation between academic field and business field for solving various problems which is occurred in plants circulation process.

• Korean Journal Plant Pathology
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• 제10권3호
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• pp.197-210
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• 1994

This experiment was carried out to study the cause of degeneration and rot of cultivated mushroom. Among 597 bacterial isolates derived from the rots of Button mushroom (Agaricus bisporus), Oyster mushroom (Pleurotus ostreatus) and Oak mushroom (Lentinus edodes) collected from markets of 5 cities (Seoul, Suwon, Taegu, Pohang and Pusan) in Korea (1991~1993), 111 bacterial isolates (18.5%) were proved as pathogenic bacteria. These pathogenic bacteria causing bacterial rots of cultivated mushrooms were identified as Pseudomonas tolasii, P. agarici, and Eriwinia sp., and the main causal bacteria were P. tolaasii. P. fluorescens and Klebsiella plenticola were confirmed as saprophytic non-pathogenic bacteria. One hundred fifty nine isolates (Group No. 39) of the 486 saprophytic bacterial isolates were classified as P. fluorescens, and this species was most often found rot area of cultivated mushrooms. P. tolaasii, the causal organism of bacterial blotch, was classified into two groups; One group can be differentiated from the other by the formation of white precipitation band by white line reacting organisms of Pseudomonas Agar F media. P. tolaasii attacked the cultivated mushrooms relatively well at lower incubation temperature such as 5$^{\circ}C$, but P. agarici rarely attack at below 1$0^{\circ}C$. The temperature for the infection commercial cultivated mushrooms by P. agarici was higher than that of P. tolaasii. Optimum temperature for the infection of mushrooms by P. tolaasii and P. agarici were 2$0^{\circ}C$ and $25^{\circ}C$, respectively.

• Journal of Broadcast Engineering
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• 제3권1호
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• pp.50-60
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• 1998

This paper addresses a problem of extraction of parameteric motion estimation and structural motion segmentation for compact image sequence representation and object-based generic video coding. In order to extract meaningful motion structure from image sequences, a direct parameteric motion estimation based on a pre-segmentation is proposed. The pre-segmentation which considers the motion of the moving objects is canied out based on probabilistic clustering with mixture models using optical flow and image intensities. Parametric motion segmentation can be obtained by iterated estimation of motion model parameters and region reassignment according to a criterion using Gauss-Newton iterative optimization algorithm. The efficiency of the proposed methoo is verified with computer simulation using elF real image sequences.

• Journal of the Korean Institute of Telematics and Electronics D
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• 제34D권4호
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• pp.1-7
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• 1997

This paper presents an analysis of the wave propagation in cross-type tunnels using the finite volume time domain (FVTD) method. Because the FVTD method is based on the volume intergrations of themaxwell's equations with respected to arbitrary shaped small polyhedron cells and the fields at every center point of the cells ar eassigned in a average fashion, the method can handle arbitrary boundary problems with inhomogeneous media. In this paper, the wave propagation in cross-type tunnels has been analyzed using the fVTD method with the PML (perfectly matched layer) absorbing bundary conditons, and the numerical results are verified with a set of experimental data.

• Journal of the Korean Society of Tobacco Science
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• 제14권1호
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• pp.3-11
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• 1992

The present study examined various environmental and cultural media conditions for in vitro "rescue" of cross-fertilized ovules formed through sexual crosses between Nicotiana rustica and N. tabacum cv. BY4. The response ovules to two cultural procedures was compared; ovules were cultured either separately or left attached to the placenta. Total yield of seedlings and percent of normal seedlings were increased by culturing individual ovules separately, rather than on excised placenta. Total yield of seedlings and number of normal seedlings were produced following in vitro culture of individual fertilized ovules of N. rustica X M tabacum cv. BY4 at four days post-pollination on NN medium containing 2% sucrose. In the in vitro culture of fertilized ovules, high sucrose concentration increased the frequency of seedlings of abnormal appearance. Therefore, sucrose should be supplied to developing ovules at gradually decreased concentrations. Culture of fertilized ovules from three to eight days after pollination gave increased number of seedlings, but with delayed cultral time the number of morphologically normal seedling were decreased. Hybrids were uniform in appearance and showed vegetative heterosis but flower characteristics were generally intermediate between those of the parents. All hvbrids evaluated were self-sterile.f-sterile.