• Analytical Science and Technology
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• v.6 no.3
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• pp.255-259
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• 1993

${\beta}$-Carboline alkaloids of Polygala tenuifolia (Polygalaceae), an expectorant, tonic and sedative drude drug, were determined by HPLC. The alkaloids were separated by RP-18 column with gradient elution of 0.01M potassium phosphate buffer(pH=3.5) and acetonitrile and detected at UV 254nm. Two methods for the preparation of alkaloid fraction were compared. One was solvent extraction method using acid and base, and the other was solid phase extraction with ion exchange resin (Amberlyst 15). A more refined HPLC chromatogram was obtained using the solid phase extraction method. The alkaloidal contents in P. tenuifolia determined by this method were : harman $2.8{\times}10^{-3}%$, norharman $1.7{\times}10^{-3}%$, 1-carbomethoxy-${\beta}$-carboline $1.3{\times}10^{-3}%$, 1-carboethoxy-${\beta}$-carboline $1.4{\times}10^{-3}%$ and perlolyrine $3.3{\times}10^{-3}%$, respectively.

• Food Science and Preservation
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• v.8 no.3
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• pp.296-301
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• 2001

Physiological functionalities in various extracts of Heugjinju rice bran were determined and its optimal extraction condition were also investigated Angiotensin-converting enzyme(ACE) inhibitory activity, fibrinolytic activity and tyrosinase inhibitory activity were higher in water extracts than those of 80% ethanol and methanol, hexane. Electron donating abilities were 97.8% in hexane extract and 83% in water extracts. ACE inhibitor was maximally extracted from Heugjinju rice bran when it was treated with 20 times of distilled water for 12 h at 20 $\^{C}$. Fibrinolytic compound was also maximally extracted by treatment of 10 times of distilled water for 18 h at 20 $\^{C}$. Electron donating compound and tyrosinase inhibitor were maximally extracted by treatment of 20 times of hexane and 10 times of distilled water at 20 $\^{C}$ for 18 h, respectively

• The Transactions of the Korea Information Processing Society
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• v.6 no.5
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• pp.1393-1402
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• 1999

This paper proposes an efficient principal feature extraction of the image data using neural networks of a new learning algorithm. The proposed learning algorithm is a backpropagation(BP) algorithm based on the steepest descent and dynamic tunneling. The BP algorithm based on the steepest descent is applied for high-speed optimization, and the BP algorithm based on the dynamic tunneling is also applied for global optimization. Converging to the local minimum by the BP algorithm of steepest descent, the new initial weights for escaping the local minimum is estimated by the BP algorithm of dynamic tunneling. The proposed algorithm has been applied to the 3 image data of 12${\times}$12pixels and the Lenna image of 128${\times}$128 pixels respectively. The simulation results shows that the proposed algorithm has better performances of the convergence and the feature extraction, in comparison with those using the Sanger method and the Foldiak method for single-layer neural networks and the BP algorithm for multilayer neural network.

• Journal of Soil and Groundwater Environment
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• v.18 no.6
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• pp.38-47
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• 2013

This study was performed to identify the optimal operating conditions and to evaluate the xylene removal efficiency, applying in-situ soil flushing with the low concentrated solution of 'Tween 80' at the xylene contaminated site. The pilot scale test site ($5m{\times}5m{\times}3m$), was mainly composed of 'sandy loam', with the average hydraulic conductivity of $9.1{\times}10^{-4}cm\;s^{-1}$. The average xylene concentration of the site was 42.1 mg $kg^{-1}$, which was more than 2.5 times higher than Korea soil pollution warning limit (15 mg $kg^{-1}$). For the soil flushing, 7,800 L of 0.1~0.2% surfactant solution was injected into three injection wells at the average injection time of 9 hr $d^{-1}$ for 10 days, followed by the additional only groundwater injection of 6,000 L. The same amount of the effluent solution was extracted from three extraction wells. From the analysis for xylene concentration of all effluent at 3 extraction wells, total 166 g of xylene was removed by in-situ surfactant flushing. Even though the residual xylene concentrations of 7 soil sampling locations in the test site were different due to the soil heterogeneity, from the comparison of xylene concentration at 7 locations before/after the feasibility test, 53.9% of the initial xylene in the site was removed from three extraction wells (mainly Ext-N and Ext-M well). The results showed that the in-situ soil flushing by using low concentrated 'Tween 80' solution had a great potential to remediate the xylene contaminated site.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.26 no.3
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• pp.307-316
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• 2016

Objectives: In general, NIOSH method 5506 is most widely used for the occupational exposure measurement of PAHs, but 2-4 ring PAHs have poor desorption efficiency, especially for a filter. The purpose of this study was to determine a method to increase the desorption efficiency of 16-PAHs using an ultrasonic extraction procedure. Methods: Test samples prepared spiked XAD-2 tubes and PTFE filters in the range of $0.01-1.0{\mu}g/mL$ for desorption efficiency study. Four different extraction solvents, acetonitrile, acetone, tetrahydrofuran and dichloromethane, were tested in order to select the most suitable solvent for the extraction of the 16 PAHs. The addition of dimethyl sulfoxide and sonication time were considered in order to determine the method with the highest extraction efficiency. All samples were made in three sets and analysis was replicated seven times by HPLC. Results: Acetonitrile and acetone were the optimized as an extraction solvent and desorption efficiency of 2-ring PAHs such as naphthalene, acenaphthylene were increased 3~19% with dimethyl sulfoxide for XAD-2. Acetone was the best extraction solvent for PTFE filter and the desorption efficiency was increased 3~13% for 2- to 4-ring PAHs. The optimum sonication time was 60 minutes and desorption efficiency increased with extraction time. Conclusions: As a result, the best extraction solvent was acetone with dimethyl sulfoxide for ultrasonic extraction procedure and the desorption efficiency of this method was better than NIOSH 5506's. This study could be applied as a method for occupational exposure measurement of PAHs.

• Journal of Ginseng Research
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• v.10 no.1
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• pp.80-93
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• 1986

The tail portion of dried 6-year old white ginseng was extracted and sugars and nitrogen compounds were also evaluated for chemical properties depending on varying conditions of extractions. The factors studied were extraction temperature in the range of 70-$100^{\circ}C$, ethanol concentration of 0-90% and the times of extractions which was taken 8 hours per each extraction in water at $80^{\circ}C$. For the effect of ethanol concentration in the extraction solvent, it was found that the amounts of free, reducing and total sugars and starch recovered in extract were almost linearly decreased along with the increase of concentration and the nonprotein nitrogen accounted over 84% of total nitrogen in extract. As ethanol concentration became increased, extractions of total nitrogen and water souluble nonprotein nitrogen were decreased especially in 90% ethanol. For the extraction temperature, all the sugar fractions with water and 70% ethanol except free sugar have tended to increase along with the temperature raised from 70 to $100^{\circ}C$ and it was found there is little changes of nitrogen compounds in the temperature range except a rapidly increase in water soulble protein at $100^{\circ}C$. For the times of extractions, showed that most of extractable compounds were extracted in 3 times of extractions with water at $80^{\circ}C$. It was shown that more than 95f) of sugars and 80% of nitrogen compounds were yielded with water extraction. Accordingly it was efficient to extract with water or 70% ethanol in 3 times in terms of !actor and energy consumption.

• Korean Journal of Medicinal Crop Science
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• v.17 no.5
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• pp.311-320
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• 2009

The low quality fresh ginseng was fermented by Phelinus linteus or Hericium erinaceum mycelium. This fermented ginseng was extracted by water at $100^{\circ}C$ or water with ultrasonification at $60^{\circ}C$. Total phenolic compounds was improved by ultrasonification extraction process, compare to conventional water extraction. All extracts enhanced the growth of human B and T cells, showing 2.68 times and 3.43 times higher, respectively, than the control. The secretion of TNF-$\alpha$ and IL-6 from human immune cells was enhanced as $3.53{\times}10^{-4}\;pg/cell$, $3.40{\times}10^{-4}\;pg/cell$ by adding H. erinaceum mycelium fermented ginseng. H. erinaceum mycelium fermented ginseng yielded higher nitric oxide production from macrophage than Lipopolysaccharides (LPS). The cytotoxicity on human normal kidney cell (HEK293) was as low as 20.5% in adding the maximum concentration of $1.0\;mg/m{\ell}$ of fermented ginseng. Generally, the extracts from ultrasonification extraction process showed 10% lower toxicity than that by conventional process. H. erinaceum mycelium fermented ginseng had the highest anticancer activity on human lung cancer and stomach cancer cells as 69.33% and 75.32%, respectively at $1.0\;mg/m{\ell}$. It can be concluded that, in general, H. erinaceum mycelium fermented ginseng has relatively better immune and anticancer activities than P. linteus fermented ginseng. Expecially, the extracts treated with ultrasonification had higher activities than that from conventional extraction process.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.1
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• pp.40-46
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• 1984

The extraction ratio of oil using solvent from the muscle of sardine, Sardinops melanosticta, was studied. The results were critically evaluated in the light of the theory of oil diffusion in a porous solid model. In addition the effect of temperature and moisture on the extraction rate was examined. Sardine muscle was prepared in a manner to meet the conditions required by the diffusion theory from Fick's law. The results of the model were well coincidenced to the theory derived from Fick's law. Diffusion constants at the direction to muscle fiber($D_1$) and at direction perpendicular to fiber($D_2=D_3$) when extracted at $45^{\circ}C$ were $8.16{\times}10^{-8}cm^2/sec\;and\;4.12{\times}10^{-8}cm^2/sec$, respectivly. The extraction rate was linearly propotional to absolute temperature(T) by eleventh power under the constant condition of moisture contents and muscle size. A comparison of the experiments with the highest($74.22\%$) and the lowest ($32.48\%$) moisture indicated that difference of $1\%$ in moisture contents caused to change the slope(K) of the extraction curve $0.53{\times}10^{-6}sec^{-1}$ approximately.

• Korean Journal of Medicinal Crop Science
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• v.16 no.5
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• pp.294-300
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• 2008

This study was performed to investigate the increasing of immune activities from Centella asiatica L. Urban by several solvent extraction with ultrasonification process. Ethanol extracts at $60^{\circ}C$ and associated with ultrasofication showed more 15% yield than normal $100^{\circ}C$ water extracts. And ethanol extracts showed higher yield than water extraction at each process. It can be concluded that yield due to polarity of compound in C. asiatica from HPLC peak. All of extracts from C. asiatica revealed lower cytotoxicity than 32%. The ethanol extracts with ultrasofication showed higher promotion on human B and T cell growth, about 10% compared to the control. The secretion of IL-6 and TNF-a was also enhanced as $4.86\;{\times}\;10^{-4}\;pg/cell$ and $5.73\;{\times}\;10^{-4}\;pg/cell$ each, by the addition ethanol extracts with ultrasonification to T cell. NK cell activation was improved up to 10% higher than the control, through adding the samples. It can be concluded that the ethanol extracts from C. asiatica has higher immune activities followed by ultrasonification process, possibly, by higher yield than conventional extraction process.

• KSBB Journal
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• v.23 no.3
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• pp.193-198
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• 2008

In this study, the supercritical $CO_2$ extraction was applied for the enrichment of coffee flavors. The extraction efficiency of coffee flavors was dependent on the pressure and temperature and optimized as 350 bar and 80$^\circ$C. Five flavors of high aroma values were analyzed. The flavors extracted by the supercritical $CO_2$ extraction were 76.6 times higher than those by the traditional method, espresso extraction. The modified headspace system proved the enhanced efficiency of supercritical $CO_2$ extraction. The coffee beverage containing the extract of supercritical $CO_2$ resulted 2.3 times of flavor enrichment when it was compared to that without the supercritical extract.