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Rapid Gas Chromatographic Screening of Vegetable Oils for Free Fatty Acids (기체크로마토그래피법에 의한 식물성 유지내 유리 지방산의 신속한 스크리닝)

  • Kim, Jung-Han;Kim, Kyoung-Rae;Chai, Jeong-Young;Oh, Chang-Hwan;Park, Hyung-Kook
    • Korean Journal of Food Science and Technology
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    • v.25 no.4
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    • pp.373-378
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    • 1993
  • A rapid gas chromatographic profiling method for the simultaneous analysis of free fatty and other acids was applied to vegetable oils. Oil samples were dissolved in dichloromethane and the free acids were extracted with saturated $NaHCO_3$ solution. The aqueous extract was acidified and then loaded onto the Chromosorb P column for the extraction. The acids were eluted with diethyl ether selectively from Chromosorb P column and were treated with triethylamine to prevent the losses of volatile acids. Several long chain fatty acids were detected from soybean oil, rice-bran oil, sesame oil and perilla oil. Various organic acids including odd number fatty acids were detected in crude oil, especially sesame oil. Arachidic acid from perilla oil and vanillic acid from sesame oil, which were not reported before were detected. The content ratio of free linoleic acid to oleic acid was $1.02{\sim}1.18$, which was similar to the reported data. When the GC profile of organic acids were simplified to their corresponding retention index spectra of bar graphical forms, they presented characteristic pattern of each vegetable oil that can be quickly recognized.

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Antioxidative Effect of us javanica Linne Extract by Various Solvents (붉나무 순차 용매 추출물의 항산화 효과 비교)

  • Lee, Yun-Jae;Shin, Dong-Hwa;Chang, Young-Sang;Kang, Woo-Suk
    • Korean Journal of Food Science and Technology
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    • v.25 no.6
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    • pp.677-682
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    • 1993
  • Hexane(Hx), ethyl acetate(EtoAc) methanol(MeOH) and 99% ethanol(EtOH) extract of Rhus javanicus Lines with synergists e.g. ascorbic acid(AA), citric acid(CA) and ${\delta}-tocopherol$(TO) were tested their antioxidative effect on palm oil and lard by Rancimat. The methanol showed the highest extraction yield as 14.53%(w/w). When each 600 ppm of Hx, EtOAc, MeOH and EtOH extract with 200 ppm of AA was added to palm oil, the antioxidative index(AI: induction time of oil containing of each extract/induction time of test oil) were 1.83, 2.25, 2.81 and 2.85 respectively which were higher than other treatments and 600 ppm of each extract with 200 ppm of TO to lard, the AI were 3.64, 7.83, 7.34 and 9.30 respectively. Each solvent fractionate of EtOH and EtOAc extracts resulted no higher antioxidative effect than crude whole extract. Palm oil and lard containing 600 ppm of methanol extract were very stable comparing with the control by POV and TBA at oven test($60^{\circ}C$).

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Antioxidative Activity of Heat Treated Licorice (Glycyrrhiza uralensis Fisch) Extracts (열처리한 감초추출물의 항산화활성)

  • Woo, Koan-Sik;Jang, Keum-Il;Kim, Kwang-Yup;Lee, Hee-Bong;Jeong, Heon-Sang
    • Korean Journal of Food Science and Technology
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    • v.38 no.3
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    • pp.355-360
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    • 2006
  • Antioxidative activity and physicochemical characteristics of heat-treated licorice extracted by ethyl-acetate (EtOAc) and ethyl-alcohol (EtOH) were evaluated at various treatment temperatures (110, 120, 130, 140, and $150^{\circ}C$), times (1, 2, 3, 4, and 5 hr), and moisture contents (10, 20, 30, 40, and 50%). Maximum extraction yields of EtOAc treated at $140^{\circ}C$, 2 hr, and 20% moisture content and EtOH extracts treated at $120^{\circ}C$, 2 hr, and 40% moisture content were 9.48 and 32.90%, whereas those of control were 3.74 and 14.60%, respectively. Highest total polyphenol content was obtained from 13.95 mg/g EtOH extract treated at $150^{\circ}C$, 3 hr, and 30% moisture content (control: 6.92 mg/g). Highest antioxidative activity $(IC_{50})$ was obtained from 0.32 g/L EtOAc treated at $140^{\circ}C$, 2 hr, and 20% moisture content (control: 0.57 g/L). Highest ascorbic acid equivalent antioxidant activity value of 2,112.61 mg ascorbic acid (AA) eq was obtained from EtOAc extract treated at $120^{\circ}C$, 2 hr, and 40% moisture content (control: 1,920.27 mg AA eq). Optimum heat treatment conditions were $130-140^{\circ}C$, 3 hr, and 30% moisture content.

A Study on the Proteolysis of Mussel Protein by a Commercial Enzyme Preparation (단백질 분해효소에 의한 홍합 단백질의 분해에 관한 연구)

  • Choi, In-Jae;Nam, Hee-Sop;Shin, Zae-Ik;Lee, Byong-Hoon
    • Korean Journal of Food Science and Technology
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    • v.24 no.6
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    • pp.519-523
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    • 1992
  • The patterns on the proteolysis of mussel protein using a commercial enzyme preparation were investigated. The best one among six commercial enzyme preparations for the manufacture of mussel extract was Corolase PP, based on the degree of hydrolysis (DH). When the raw mussel paste, without water addition, was adjusted to pH 6.5, added 0.1% (w/w dry basis) of Corolase PP. and reacted at $50^{\circ}C$ for four hours, it reached the maximum value of DH (79%). The precooking of raw mussel decreased the efficiency of extraction and hydrolysis of the protein, due to the inactivation of the autolytic enzymes contained in the mussel. During the course of proteolysis, major free amino acids such as glycine, alanine, glutamic acid and lysine, representing a characteristic brothy taste of mussel were replaced with free hydrophobic amino acids including valine, methionine, isoleucine, and leucine. The electrophoretic pattern and HPLC-GPC pattern of mussel protein hydrolysates during the hydrolysis were observed and also discussed.

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The effect of LH Receptor in the Pregnancy of Poor Responders (황체호르몬 수용체의 발현이 저반응 환자군의 임신에 미치는 영향)

  • Lee, Jung-Bok;Do, Byung-Rok;Kim, Eun-Soo;Kim, Myung-Hee;Chun, Eun-Kyung;Jeong, Hyeon-Jeong;Roh, Sung-Il;Kim, Moon-Kyoo;Yoon, Hyun-Soo
    • Clinical and Experimental Reproductive Medicine
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    • v.31 no.2
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    • pp.111-117
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    • 2004
  • Objectives: To investigate the effect of LH receptor in folliculogenesis, we confirm the expression level of LH receptor (LH-R) mRNA in human granulosa cells (GCs) and its expression levels were analyzed by comparison to embryo developmental rate and pregnancy rate. Materials and Methods: GCs were obtained at the time of oocyte retrieval from the patients undergoing IVF-ET program. The patients were divided into two groups: Group I (n=20) is poor responder (retrieved oocyte(s)$\leq$3ea), Group II (n=80) is normal responder (retrieved oocytes>3ea). After the extraction of total RNA, semiquantitative RT-PCR was performed and the expression level of LH-R mRNA was normalized by $\beta$-actin. Statistical analysis was performed by using $X^2$ test, Student's t-test and Pearson correlation. Results: In Group II, the relative values of LH-R mRNA (0.680 vs. 0.463, p<0.005) and pregnancy rate (54.7% vs. 23.1%, p<0.05) were significantly higher than in Group I. Number of retrieved oocyte(s) was gradually increased when the expression of LH-R mRNA was increased (p<0.05). But the quality of retrieved oocyte and transferred embryo were not related with the expression of LH-R mRNA. When the pregnancy rate was compared with FSH only group and FSH combined with hMG group in the ovarian stimulation protocol, FSH combined with hMG group was significantly higher than FSH only group in Group I (37.5% vs. 0%), and the expression of LH-R mRNA was significantly higher in hMG combined group than FSH only group (p<0.05). Conclusion: Expression level of LH-R mRNA has important role in ovarian function related with the response to gonadotrophin in human folliculogenesis. Furthermore these data might provide the evidence that additional use of hMG is helpful to poor responders.

Corpus-based Korean Text-to-speech Conversion System (콜퍼스에 기반한 한국어 문장/음성변환 시스템)

  • Kim, Sang-hun; Park, Jun;Lee, Young-jik
    • The Journal of the Acoustical Society of Korea
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    • v.20 no.3
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    • pp.24-33
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    • 2001
  • this paper describes a baseline for an implementation of a corpus-based Korean TTS system. The conventional TTS systems using small-sized speech still generate machine-like synthetic speech. To overcome this problem we introduce the corpus-based TTS system which enables to generate natural synthetic speech without prosodic modifications. The corpus should be composed of a natural prosody of source speech and multiple instances of synthesis units. To make a phone level synthesis unit, we train a speech recognizer with the target speech, and then perform an automatic phoneme segmentation. We also detect the fine pitch period using Laryngo graph signals, which is used for prosodic feature extraction. For break strength allocation, 4 levels of break indices are decided as pause length and also attached to phones to reflect prosodic variations in phrase boundaries. To predict the break strength on texts, we utilize the statistical information of POS (Part-of-Speech) sequences. The best triphone sequences are selected by Viterbi search considering the minimization of accumulative Euclidean distance of concatenating distortion. To get high quality synthesis speech applicable to commercial purpose, we introduce a domain specific database. By adding domain specific database to general domain database, we can greatly improve the quality of synthetic speech on specific domain. From the subjective evaluation, the new Korean corpus-based TTS system shows better naturalness than the conventional demisyllable-based one.

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Web Site Keyword Selection Method by Considering Semantic Similarity Based on Word2Vec (Word2Vec 기반의 의미적 유사도를 고려한 웹사이트 키워드 선택 기법)

  • Lee, Donghun;Kim, Kwanho
    • The Journal of Society for e-Business Studies
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    • v.23 no.2
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    • pp.83-96
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    • 2018
  • Extracting keywords representing documents is very important because it can be used for automated services such as document search, classification, recommendation system as well as quickly transmitting document information. However, when extracting keywords based on the frequency of words appearing in a web site documents and graph algorithms based on the co-occurrence of words, the problem of containing various words that are not related to the topic potentially in the web page structure, There is a difficulty in extracting the semantic keyword due to the limit of the performance of the Korean tokenizer. In this paper, we propose a method to select candidate keywords based on semantic similarity, and solve the problem that semantic keyword can not be extracted and the accuracy of Korean tokenizer analysis is poor. Finally, we use the technique of extracting final semantic keywords through filtering process to remove inconsistent keywords. Experimental results through real web pages of small business show that the performance of the proposed method is improved by 34.52% over the statistical similarity based keyword selection technique. Therefore, it is confirmed that the performance of extracting keywords from documents is improved by considering semantic similarity between words and removing inconsistent keywords.

Normalized Cross Correlation-based Multiview background Subtraction for 3D Object Reconstruction (3차원 객체 복원을 위한 정규 상관도 기반 다중 시점 배경 차분 기법)

  • Paeng, Kyunghyun;Hwang, Sung Soo;Kim, Hee-Dong;Kim, Sujung;Yoo, Jisung;Kim, Seong Dae
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.6
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    • pp.228-237
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    • 2013
  • In this paper, we propose a normalized cross correlation(NCC)-based multiview background subtraction method which is robust when an object and background have similar color. When the background of the capturing environment is not artificially composed, the regions in the background images which would be occluded by an object tends to have difference colors. The colors of those regions, however, becomes similar when an object enters the capturing environment. Based on this assumption, this paper proposes a concept of GoNCC(Graph of Normalized Cross Correlation). GoNCC is the distribution of NCC between a pixel in an image and pixels related by epipolar constraints with the pixel. The proposed multiview background subtraction method is performed by comparing GoNCC of the current images with the background images. To reduce computational complexity, we perform multiview background subtraction only to the pixels undetermined by single view background subtraction. Experimental results show that the proposed method is more robust to color similarity between an object and background than a single-view background subtraction method and a previous multiview background subtraction method.

High-resolution 3D Object Reconstruction using Multiple Cameras (다수의 카메라를 활용한 고해상도 3차원 객체 복원 시스템)

  • Hwang, Sung Soo;Yoo, Jisung;Kim, Hee-Dong;Kim, Sujung;Paeng, Kyunghyun;Kim, Seong Dae
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.10
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    • pp.150-161
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    • 2013
  • This paper presents a new system which produces high resolution 3D contents by capturing multiview images of an object using multiple cameras, and estimating geometric and texture information of the object from the captured images. Even though a variety of multiview image-based 3D reconstruction systems have been proposed, it was difficult to generate high resolution 3D contents because multiview image-based 3D reconstruction requires a large amount of memory and computation. In order to reduce computational complexity and memory size for 3D reconstruction, the proposed system predetermines the regions in input images where an object can exist to extract object boundaries fast. And for fast computation of a visual hull, the system represents silhouettes and 3D-2D projection/back-projection relations by chain codes and 1D homographies, respectively. The geometric data of the reconstructed object is compactly represented by a 3D segment-based data format which is called DoCube, and the 3D object is finally reconstructed after 3D mesh generation and texture mapping are performed. Experimental results show that the proposed system produces 3D object contents of $800{\times}800{\times}800$ resolution with a rate of 2.2 seconds per frame.

Application of CSP Filter to Differentiate EEG Output with Variation of Muscle Activity in the Left and Right Arms (좌우 양팔의 근육 활성도 변화에 따른 EEG 출력 구분을 위한 CSP 필터의 적용)

  • Kang, Byung-Jun;Jeon, Bu-Il;Cho, Hyun-Chan
    • Journal of IKEEE
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    • v.24 no.2
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    • pp.654-660
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    • 2020
  • Through the output of brain waves during muscle operation, this paper checks whether it is possible to find characteristic vectors of brain waves that are capable of dividing left and right movements by extracting brain waves in specific areas of muscle signal output that include the motion of the left and right muscles or the will of the user within EEG signals, where uncertainties exist considerably. A typical surface EMG and noninvasive brain wave extraction method does not exist to distinguish whether the signal is a motion through the degree of ionization by internal neurotransmitter and the magnitude of electrical conductivity. In the case of joint and motor control through normal robot control systems or electrical signals, signals that can be controlled by the transmission and feedback control of specific signals can be identified. However, the human body lacks evidence to find the exact protocols between the brain and the muscles. Therefore, in this paper, efficiency is verified by utilizing the results of application of CSP (Common Spatial Pattern) filter to verify that the left-hand and right-hand signals can be extracted through brainwave analysis when the subject's behavior is performed. In addition, we propose ways to obtain data through experimental design for verification, to verify the change in results with or without filter application, and to increase the accuracy of the classification.