Browse > Article

The effect of LH Receptor in the Pregnancy of Poor Responders  

Lee, Jung-Bok (Department of Medical Research Center, MizMedi Hospital)
Do, Byung-Rok (Department of Medical Research Center, MizMedi Hospital)
Kim, Eun-Soo (Department of Medical Research Center, MizMedi Hospital)
Kim, Myung-Hee (Department of Medical Research Center, MizMedi Hospital)
Chun, Eun-Kyung (Department of Medical Research Center, MizMedi Hospital)
Jeong, Hyeon-Jeong (Department of Medical Research Center, MizMedi Hospital)
Roh, Sung-Il (Department of Medical Research Center, MizMedi Hospital)
Kim, Moon-Kyoo (Department of Life Science, College of Natural Sciences, Hanyang University)
Yoon, Hyun-Soo (Department of Medical Research Center, MizMedi Hospital)
Publication Information
Clinical and Experimental Reproductive Medicine / v.31, no.2, 2004 , pp. 111-117 More about this Journal
Abstract
Objectives: To investigate the effect of LH receptor in folliculogenesis, we confirm the expression level of LH receptor (LH-R) mRNA in human granulosa cells (GCs) and its expression levels were analyzed by comparison to embryo developmental rate and pregnancy rate. Materials and Methods: GCs were obtained at the time of oocyte retrieval from the patients undergoing IVF-ET program. The patients were divided into two groups: Group I (n=20) is poor responder (retrieved oocyte(s)$\leq$3ea), Group II (n=80) is normal responder (retrieved oocytes>3ea). After the extraction of total RNA, semiquantitative RT-PCR was performed and the expression level of LH-R mRNA was normalized by $\beta$-actin. Statistical analysis was performed by using $X^2$ test, Student's t-test and Pearson correlation. Results: In Group II, the relative values of LH-R mRNA (0.680 vs. 0.463, p<0.005) and pregnancy rate (54.7% vs. 23.1%, p<0.05) were significantly higher than in Group I. Number of retrieved oocyte(s) was gradually increased when the expression of LH-R mRNA was increased (p<0.05). But the quality of retrieved oocyte and transferred embryo were not related with the expression of LH-R mRNA. When the pregnancy rate was compared with FSH only group and FSH combined with hMG group in the ovarian stimulation protocol, FSH combined with hMG group was significantly higher than FSH only group in Group I (37.5% vs. 0%), and the expression of LH-R mRNA was significantly higher in hMG combined group than FSH only group (p<0.05). Conclusion: Expression level of LH-R mRNA has important role in ovarian function related with the response to gonadotrophin in human folliculogenesis. Furthermore these data might provide the evidence that additional use of hMG is helpful to poor responders.
Keywords
Folliculogenesis; LH-R; Poor responder; Pregnancy rate;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Fevold HL. Synergism of follicle stimulating and luteiniznig hormone in producing oestrogen secretion. J Clin Endocrinol Metab 1941; 21
2 Levy D, Navarro J, Schattman G, Davis OK, Rosenwaks Z. The role of LH in ovarian stimulation: exogenous LH: let's design the future. Hum Reprod 2000; 15: 2258-65   DOI   ScienceOn
3 Erickson GF, Hsueh AJW. Stimulation of aromatase activity by follicle stimulatin hormone in rat granulose cells in vivo and in vitro. Endocrinology 1978; 102: 1275-83   DOI   ScienceOn
4 Beckers NG, Macklon NS, Eijkemans MJ, Fauser BC. Women with regular menstrual cycles and a poor response to ovarian hyperstimulation for in vitro fertilization exhibit follicular phase characteristics suggestive of ovarian aging. Fertil Steril 2002; 78: 291-7   DOI   ScienceOn
5 De Placido G, Mollo A, Alviggi C, Strina I, Varricchio MT, Ranieri A et al. Rescue of IVF cycles by HMG in pituitary down-regulated normogonadotrophic young women characterized by a poor initial response to recombinant FSH. Hum Reprod 2001; 16 : 1875-9   DOI   ScienceOn
6 Kligman I, Rosenwaks Z. Differentiating clinical profiles: predicting good responders, poor responders, and hyperresponders. Fertil Steril 2001; 76: 1185-90   DOI   ScienceOn
7 Thiruppathi P, Shatavi S, Dias JA, Radwanska E, Luborsky JL. Gonadotrophin receptor expression on human granulosa cells of low and normal responders to FSH. Mol Hum Reprod 2001; 7: 697-704   DOI   ScienceOn
8 Benadiva C, Ben-Rafael Z, Strauss JF 3rd, Mastroianni L Jr, Flickinger GL. Ovarian response of individuals to different doses of human menopausal gonadotropin. Fertil Steril 1988; 49: 997-1001   DOI   PUBMED
9 Schoot DC, Coelingh Bennink HJ, Mannaerts BM, Lamberts SW, Bouchard P, Fauser BC. Human recombinant follicle-stimulating hormone induces growth of preovulatory follicles without concomitant increase in androgen and estrogen biosynthesis in a woman with isolated gonadotropin deficiency. J Clin Endocrinol Metab 1992; 74: 1471-3   DOI
10 Scott RJ. Evaluation and treatment of low responders. Semin Reprod Endocrinology 1996; 14: 317-37   DOI
11 Neulen J, Wenzel D, Hornig C, Wunsch E, Weissenborn U, Grunwald K et al. Poor responder-high responder: the importance of soluble vascular endothelial growth factor receptor 1 in ovarian stimulation protocols. Hum Reprod 2001; 16: 621-6   DOI   ScienceOn
12 Garcia JE, Jones GS, Acosta AA, Wright G. HMG/HCG follicular maturation for oocytes aspiration: phase II. Fertil Steril 1983; 39: 174-9   PUBMED
13 Land JA, Yarmolinskaya MI, Dumoulin JC, Evers JL. High-dose human menopausal gonadotropin stimulation in poor responders does not improve in vitro fertilization outcome. Fertil Steril 1996; 65: 961-5   PUBMED
14 de Boer EJ, den Tonkelaar I, te Velde ER, Burger CW, Klip H, van Leeuwen FE. A low number of retrieved oocytes at in vitro fertilization treatment is predictive of early menopause. Fertil Steril 2002; 77 : 978-85   DOI   ScienceOn
15 Forti G, Krausz C. Evaluation and treatment of the infertile couple. Fertil Steril 1998; 83: 4177-88
16 Karande V, Morris R, Reinehart J, Miller C, Rao R, Gleicher N. Limited success using the "flare" protocol in poor responders in cycles with low basal follicle-stimulating hormone levels during in vitro fertilization. Fertil Steril 1997; 67: 900-3   DOI   ScienceOn
17 Vargyas J, Morente C, Shangold G. The effect of different methods of ovarian stimulation for human in vitro fertilization and embryo replacement. Fertil Steril 1984; 42: 745-7   DOI
18 Short RV. Steroids in the follicular fluid and the corpus luteum of the mare. A 'two-cell type' theory of ovarian steroid synthesis. J Endocrinology 1962; 24: 59-63   DOI   ScienceOn
19 Saith RR, Srinivasan A, Michie D, Sargent IL. Relationship between the developmental poteitial of human in-vitro fertilization embryos and features describing the embryo, oocyte and follicle. Hum Reprod Update 1998; 4: 121-34   DOI   ScienceOn
20 Nikolaou D, Lavery S, Turner C, Margara R, Trew G. Is there a link between an extremely poor response to ovarian hyperstimulation and early ovarian failure?Hum Reprod. 2002; 17: 1106-11
21 Rao CV. Multiple novel roles of luteinizing hormone. Fertil Steril 2001; 76: 1097-100   DOI   ScienceOn
22 Quintana R, Kopcow L, Marconi G, Sueldo C, Speranza G, Baranao RI. Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis. Hum Reprod 2001; 16: 1814-8   DOI   ScienceOn
23 Filicori M. The role of lutenizing hormone in folliculogenesis and ovulation induction. Fertil Steril 1999; 71: 405-14   DOI   ScienceOn
24 Tarlatzis BC, Zepiridis L, Grimbizis G, Bontis J. Clinical management of low ovarian response to stimulation for IVF: a systematic review. Clinical management of low ovarian response to stimulation for IVF: a systematic review. Hum Reprod Update 2003; 9: 61-76   DOI   ScienceOn
25 Keay SD, Liversedge NH, Mathur RS, Jenkins JM. Assisted conception following poor ovarian response to gonadotrophin stimulation. Br J Obstet Gynaecol 1997; 104: 521-7   DOI   PUBMED