• 한국조리학회지
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• 제22권2호
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• pp.151-162
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• 2016

본 연구에서는 무우액종 천연발효 빵 선택동기가 소비자태도와 구매의도에 미치는 영향요인들과 그 요인을 이용한 앞으로의 활용방안을 제시하고자 한다. 총 300부의 설문지를 배포하여 불성실하게 응답한 21부의 설문지를 제외한 나머지 279부의 유효한 설문지를 실증연구에 사용되었다. 조사 연구의 목적을 달성하기 위하여 통계프로그램 SPSS 18.0을 활용하여 빈도분석, 요인분석 및 신뢰도분석, 상관관계분석, 회귀분석을 실시하였다. 분석결과를 보면 선택동기에 대한 측정항목의 탐색적 요인분석 결과, 3개 요인으로 KMO 값은 0.735, 총분산비율 65.759%, 소비자태도에 대한 요인분석은 총분산비율 72.421%, KMO 값은 0.684로 나타났다. 구매의도에 대한 요인분석은 총분산설명력 67.139%, KMO 값은 0.683으로 나타났다. 변수들 간의 상관관계가 다른 변수에 의해 설명되는 정도가 좋게 분석되었고, 유의확률이 0.000으로 나타나 전반적으로 변수들 간의 상관관계는 유의적이다. 따라서 선택동기에 따른 소비자태도는 구매의도에 미치는 영향에 관한 가설은 채택되었다.

• Journal of Applied Biological Chemistry
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• 제57권3호
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• pp.197-203
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• 2014

Various parameters such as solvent selection, concentration, solid/liquid ratio, soaking time, temperature, stationary, shaking conditions, and repeated extractions were investigated in order to determine the optimum extraction conditions of ${\beta}$-glucosidase from bagasse fermented by mixed culture of Aspergillus niger NRC 7A and Aspergillus oryzae NRRL 447. Among various solvents tested, non ionic detergents gave the best results than the inorganic or organic salt solutions and distilled water. The optimum conditions for extraction of ${\beta}$-glucosidase were 30 min soaking time at $40^{\circ}C$ under shaking condition at 150 rpm, with solid/liquid ratio 1:15 (w/v), which yielded $2882.74{\pm}95.52U/g$ fermented culture (g fc) of enzyme activity. With repeated washes under the above optimum conditions, the results showed that enzyme extracted in the $1^{st}$ and $2^{nd}$ washes represents about 90% of the total activity.

• 전자공학회논문지B
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• 제29B권11호
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• pp.22-30
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• 1992

Using Multi-Dimentional Scaling(MDS) method, this paper analyzes the differences of acoustic properties of Korean phonemes projected as distances on a plan space. The phonemes were extracted from mono-syllables frequently occurring in daily conversation. From the distances between vowels we found that the distances between vowels /∂/ and /w/, /o/ and /u/, and vowels /$\varepsilon$/ and /e/ were relatively too short separate automatically. From the analysis of consonants. we found short distances between 1) phonemes in each phoneme group, 2) nasals and liquid /r/ of work initial, 3) nasal /n,m/ and liquid /l/ of word finals. But nasals, liquids and plosives of word initials had enough distances to be separated from those of word finals in automatic recogniation.

• 동력기계공학회지
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• 제20권1호
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• pp.42-49
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• 2016

This study showed that on-site ferrate(VI) solution was synthesized by wet oxidation method and applied aqueous 2,4-dichlorophenol(DCP) solution to evaluate the degradation efficiency. On-site ferrate(VI) solution was synthesized by putting $FeCl_3{\cdot}6H_2O$ in the strong alkali solution with NaClO and NaOH and applied DCP solution directly. DCP solution was extracted by the liquid-liquid method and analyzed by GC-ECD. The factors such as pH, DCP initial concentration, injected ferrate(VI) dosage, temperature were investigated. The optimum pH and temperature conditions of DCP degradation were obtained in neutral condition and $35^{\circ}C$. And the experimental results showed that DCP removal efficiency also increased with the decrease of DCP initial condition and the injected ferrate(VI) dosage.

• 약학회지
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• 제40권2호
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• pp.149-154
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• 1996

A high-performance liquid chromatographic method for the determination of ${\beta}$-sitosterol in the unsaponifiable fraction of Zea mays L. and its related drug preparations using a cholesterol as an internal standard was investigated. They were saponified with 20% methanolic KOH solution. Phytosterols in the reaction mixture were extracted with diethyl ether and separated on silica gel TLC plate with n-hexane-diethyl ether(40:60) as the solvent and then were scraped off. They were separated by reversed phase high perfomance liquid chromatography on Inertsil ODS-2 column with detection at 205nm. Cholesterol and ${\beta}$-sitosterol were resolved from interferences by adjusting the acetonitrile content in the MeOH-tetrahydrofuran-$H_2O$ eluent. The detection limit of ${\beta$-sitosterol was 0.43${\mu}$g.

• 미생물학회지
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• 제24권4호
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• pp.370-376
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• 1986

From the outer membrane portion of Gram-negative Klebsiella pneumoniae, the activity of 2-furaldehyde dehydrogenase depending upon beta-nicotinamide adenine dinucleotide was detected. Cytoplasmic membrane was preferentially extracted from crude membrane with $Mg^{2+}$ and Triton X-100, and then outer membrane was collected by ultracentrifugation. The crude enzyme was obtained by solubilization of outer membrane with lysozyme, ethylene diamine tetraacetate and Triton X-100. Thereafter 2-furaldehyde dehydrogenase was partially purified through column chromatography on QAE-Sephadex Q-50 and Sephadex G-150 and the enzyme activity was analyzed by means of high performance liquid chromatography. The optimal pH for the activity of the enzyme was about 9.5 and the optimal temperature was about $85^{\circ}C$. The partially purified enzyme retained tis activity at $85^{\circ}C$ for 5 hours. The optimal concentration of Triton X-100 for the activity of the enzyme was about 1.5% in the reaction mixture.

• 한국의류산업학회지
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• 제1권4호
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• pp.363-369
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• 1999

Artemisia princeps was used for the natural dye stuff extraction and concentration to produce the condensed dye liquid. Using this liquid, the silk and the cotton fabrics were dyed. And then the K/S value, color difference were measured in order to determine the dyeability and the antimicrobial activity. The K/S value of silk were much higher than those of cotton. In terms of the silk fabric, the premordanting method was most efficient of the mordanting method. The various surface colors on the dyed fabric were resulted according to used mordants and mordanting methods. The dye fastness was significantly improved when mordants were added. Among them, Cr-mordant improved more than 1 level. The Cu-mordant showed the greatest antimicrobial activity on both of mordant treated silk and cotton.

• 한국동물위생학회지
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• 제21권3호
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• pp.261-266
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• 1998

A method for the determination of noboviocin in milk was presented by high performance liquid chromatography(HPLC). The novobiocin in the spiked sample was extracted with methanol and evaporated under vacuum. After evaporating, the residue was mixed with distilled water for 2$m\ell$, filtrates with 0.45$\mu\textrm{m}$ acrodisc was injected into HPLC. The results obtained were as follows ; 1. The calibration curve of novobiocin was showed constantly linear(r 0.999) in the range of 100~500ng/$m\ell$. 2. The mean recovery rate of novobiocin from the spiked milk sample were 88~98%. 3. The coefficients of variation were 2.6~5.8% 4. The lowest detectable limit of novobiocin was 25ppb.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.554-557
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• 2002

Antioxidative noudle products were developed with mixed material and antioxidative liquid extracted from natural products. Matrrials were mixed with wheat flour, rice flour, starch and green tea. 3% of green tea was suitable mixing ratio from the mastication data of texture meter, and proper water contents were 33% to prepare these noodles. The suitable mixing ratio of materials were wheat flour(85%), starch( 12%) and greentea(3%) from the physical data of texture meter. Since AUC value of antioxidative liquid was more higher than that of tap water, antioxidative noodle products were expected for good health and preservation of some diseases.

• Applied Biological Chemistry
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• 제23권2호
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• pp.106-114
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• 1980

제주산 밀감 juice의 향기성분을 질소 gas로 추출하여 gas liquid chromatography로 동정하였고 각 성분의 extraction coefficient를 사용하여 함량을 구하였다. 50여개의 peak중 26성분을 동정하였다. 향기성분중 ethanol이 140ppm이며 limonene이 120ppm으로 이 두 성분이 가장 중요한 성분으로 밝혀졌다.