• 한국가정과학회지
• /
• 제8권4호
• /
• pp.5-12
• /
• 2005

The Purpose of this study is to investigate dyeing properties of fabrics with rhubarb colorants. Rhubarb colorants were extracted with water from sliced dry root of rhubarb. Using rhubarb extract, wool fabric specimens were dyed under various conditions(temperature, time, pH, mordants). Effects of dyeing conditions and mordanting on dyeing properties and colorfastness were studied. The color of the dyed fabric specimens were predominantly yellow for all of the pH range. The optimum condition for dyeing with rhubarb extract was dyeing at $80^{\circ}C$ for 30minutes. Al of 4 kinds of mordating agents and pre-mordanting showed the highest chroma value. Washfastness was 3-4, but the dyed wool fabrics became darker on light exposure.

• 대한본초학회지
• /
• 제36권4호
• /
• pp.31-39
• /
• 2021

Objectives : The purpose of this study was to find out the efficacy of ginseng on the skin by comparing the difference effect between only MTS (microneedle therapy system) and the ginseng solution application after MTS treatment. Methods : The present author performed once a week and total 3 times of MTS treatment. The treatment time took about 5~10 minutes and the depth of the needle was adjusted according to the participant's skin condition. In the control group, skin toner was applied after MTS and in the experimental group, ginseng extract dissolved toner was applied. One week after each treatment, the change of skin condition was measured. Two weeks after the end of the clinical trial, the skin condition was checked again. Results : In the case of t-zone oil, there was a significant decrease in the test group and in the case of pores and pigmentation, there was a significant decrease in both the test group and the control group, but the degree of significance was greater in the test group. In the case of skin tone, there was an increase, but no significance. Conclusion : The MTS treatment itself showed good effects on t-zone oil, pores, pigmentation, and skin tone. But the effect was much better when the ginseng extract solution was applied to the skin after MTS treatment. This study suggests that ginseng solution increases the efficacy of MTS.

• 한국식품영양과학회지
• /
• 제23권4호
• /
• pp.698-703
• /
• 1994

In spore rec-assay using B. subtillus H17(rec) and M 45(rec) , the ethanol extract of buckwheat leaves showed antimutagenicity in condition of low concentrations, but its did comutagenicity in condition of high concentrations. In Ames test, the ethanol extract of buckwheat leaves reduced the mtabenicity of N-methyl-N' -nitro-N-nitrosoguaidine (MNNG), benzo (a) pyrene(B($\alpha$)P), 2-amino-fluorene(2AF), and 3-amino -1, 4-dime-thyl-5-H-pyrido(4, 3-b) indol(Trp-P-1) in Salmonella typhimurium TA98 and TA100. The ethanol extract was fractionated by hexane, ethylacetate, butanol and water. Among Them hexane fraction showed the highest inhibition rate on the mutagenicity of B($\alpha$)P, and so did chloroform fraction on the mutagenicity of MNNG in S. typhimurium Ta98 and TA100. To elucidate the antimutagenic mechanism of the ethanol extract, it was mixed and co-incubated with various metagens, S9 mix, and the bacteria with different experimental orders and different reaction times. The ethanol extract did not affect reversion rate of pre-mutated. S.typhimurium. However, when the ethanol extract was added to the mutagens before their interaction with S.typhimurium , it reduced the mutation rate to 152$\pm$12-273$\pm$18 colonies/plates in case of MNNG, and 135$\pm$13-195$\pm$10 colonies/ plates in case of B($\alpha$)P), showing strong desmutagenic activity.

• Preventive Nutrition and Food Science
• /
• 제15권4호
• /
• pp.255-261
• /
• 2010

Ischemic stroke, a major cause of death and disability worldwide, is caused by occlusion of cerebral arteries that, coupled with or without reperfusion, results in prolonged ischemia (hypoxia and hypoglycemia) and, ultimately, brain damage. In this study, we examined whether methanol extract of the whole plant of Cassia mimosoides var. nomame Makino that grows naturally in Korea, as well as Japan and China, and some of its fractions obtained by partitioning with organic solvents could protect human hepatocellular carcinoma cells (HepG2) under hypoxic condition by inhibiting apoptosis. We also investigated if these extracts could attenuate brain damage in a rat model of 2 hr of ischemia, generated by middle cerebral artery occlusion, and 22 hr of reperfusion. The whole extract ($100{\mu}g$/mL) maintained the cell number at more than half of that initially plated, even after 24 hr of cell culture under hypoxic condition (3% $O_2$). In the absence of the whole extract, almost all of the cells were dead by this time point. This improvement of cell viability came from a delay of apoptosis, which was confirmed by observing the timing of the formation of a DNA ladder when assessed by gel electrophoresis. Of fractions soluble in hexane, ethyl acetate (EA), butanol and water, EA extracts were selected for the animal experiments, as they improved cell viability at the lowest concentration ($10{\mu}g$/mL). The whole extract (200 mg/kg) and EA extract (10 and 20 mg/kg) significantly reduced infarct size, a measure of brain damage, by 34.7, 33.8 and 45.2.0%, respectively, when assessed by 2,3,5-triphenyl tetrazolium chloride staining. The results suggest that intake of Cassia mimosoides var. nomame Makino might be beneficial for preventing ischemic stroke through inhibition of brain cell apoptosis.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2001년도 추계학술발표대회
• /
• pp.853-854
• /
• 2001

The purpose of the present study is to find the optimal growth condition of Crypthecodinium cohnii. Using a design of experiments, it was found that the yeast extract was the most significant factor for cell growth. The effect of glucose was less than that of yeast extract and the effect of NaCl was negligible. The highest cell density, 1O.96g/L was obtained at the concentration of yeast extract 7.5g/L.

• 대한가정학회지
• /
• 제41권2호
• /
• pp.107-121
• /
• 2003

This study was discussed the dyeing of wool and nylon fabrics with Chinese Scholar Tree extract. The extracts of Chinese Scholar Tree was prepared in the condition of heating at $95{\pm}5C$, for 1 hour and cooling to 40C. And then the extracts of color matter treate with vacuum concentration at $60{\pm}2^{\circ}C$, 30mmHg and dried with spray dryer. The dyeing of wool and nylon fabric in this experiment was also employed the mordant dyeing method such as pre-mordant, post-mordant and simultaneous mordant method. The mordanting agents used in this study were as followings ; aluminium potassium sulfate, copper(II) acetate monohydrate, chromium potassium sulfate$.$$7H_2O$, Tin(II) chloride dihydrate, iron(II)sulfate $7H_2O$. For an evaluation of the dyeing property of the mordanting agents, the pre-mordant method, the repeat dyeing and the fastness of the light, dry cleaning, washing and rubbing measured respectively. From the results of the dye absorption, the optimum dyeing condition of the wool and nylon fabrics with Chinese Scholar Tree extract is at 80C dyeing temperature for 60 minutes. The optimum concentration of mordanting agent is Al, Cr, Sn 1%, Fe, Cu 2% solution. In general, the fastness property of the dyed wool and nylon fabrics had a comparatively high grade.

• 한국식품영양과학회지
• /
• 제23권6호
• /
• pp.1001-1007
• /
• 1994

In order to develop a natural food preservative, dried salviae miltiorrhizae radix (Salvia miltiorrhiza) was extracted with several solvents, and then antimicrobial activity was investigated. The optimum extracting condition for the antimicrobial sustance from the sample, minimal inhibitory concentration (MIC) of the extracted substance against microorganisms were also examined. Antimicrobial activity of the initial ethanol extract from the sample was the strongest compared to those of other solvent extracts such as n-hexane, acetone, butanol, methanol and water. the optimum extractingcondition for antimicrobial substance from the sample was shaking extraction for 2 hours at room temperature incase that 10 volumes of absolute ethanol was added to crushed Saliva Miltiorrhiza. The ethanol extract had strong growth inhibition activity against Gram-positive Bacteria (MIC, 3.13-50$\mu\textrm{g}$/ml) such as B. cereus, B, subtilis, L. minocytogenes, S. aureus, Sc. Mutans. Among Grampositive bacteria tested, Bacillus species was the most susceptibile to the extracted substance. The antimicrobial activity of the ethanol extract from the sample was weak to Gram -negative bacteria yeasts, for example MIC for Gram-negative bacteria and yeasts was 0.8mg/ml and 0.4-0.8mg/ml , respectively.

• 한국식품영양과학회지
• /
• 제29권2호
• /
• pp.307-311
• /
• 2000

The purpose of this study was to identify excellent immune-enhancing substance from Korean wheats(Eunpa, Gueru, Alchan, Topdong, Suwon 267, Gobun) compared with imported ones(Australian standard white, ASW; Dark northern spring, DNS). Phagocytic activities of PBS (phosphate buffered saline, pH 7.4) and EA(ethanol-acetic acid) extracts from the wheats were determined using mouse macrophage J774 cell line. In order to set the optimal experimental condition up, the cultured cells were tested in varying experimental conditions. About two to five times higher phagocytic activity was shown in EA extract of Korean wheats compared to that of imported wheats. PBS extracts of wheats did not show increased phagocytic activity compared to control that did not add any extract. The EA extract of Gobun wheat showed the highest phagocytic activity. From the experiment we found that the optimal experimental condition was shown in two hours of reaction time and 0.05mg amout of EA extract added to J774 cells.

• 생명과학회지
• /
• 제11권4호
• /
• pp.321-327
• /
• 2001

The purpose of this study was to investigate the nitrite scavenging activity of the ethanol and water extracts obtained from Hizikia fusiforme and Sargassum fulvelum using sodium nitrite under different pH conditions. The nitrite scavenging activity was appeared in all the extracts examined and it showed the highest value at pH 1.2 The ethanol extract of Hizikia fusiforme provided higher activity than the water extract of that. Ohterwise, the nitrite scavenging activity of Sargassum fulvelum ethanol extract was similar to that Sargassum fulvelum water extract. There was significant difference among concentration of extracts Hizikia fusiforme and Sargassum fulvelum. Also, nitrite scavenging activity of the extracts Hizikia fusiforme and Sargassum fulvelum decreased with higher pH condition. In addition, the activity was increaed with higher concentration of extracts Hizikia fusiforme and Sargassum fulvelum in the course of reaction time 30 minute to 3 hour.

• 대한의생명과학회지
• /
• 제10권3호
• /
• pp.317-324
• /
• 2004

In order to examine the clinical effects on male pattern hair loss, the oriental medicine extract composed of Polygoni multiflori Radix, Angelica gigantis Radix and Lycii Fructus was tested to balding people. 40 males and 10 females were participated in this experiment, and they had spread the extract on their scalp themselves one time a day for 18 months. After 18 months, all participants submitted the reports that had questionnaires to determine the effect of topical oriental medicine extract on hair growth and/or hair loss preventing in balding people. In the results, ir was found that hair density on the entire scalp was gradually increased during the spreading period of the extract in most of balding people. The hair loss was observed about 1 week or 2 months after extract treatment and hair growth was observed about 6 months after extract treatment. In the reports, 74％ of participants answered that the extract was effective on hair growth and hair loss preventing, and improvement of scalp condition. From these results, it is suggested that the extract composed of Polygoni multiflori Radix, Angelica gigantis Radix and Lycii Fructus may be an effective medical care to hair growth and hair loss preventing in human.