• The Journal of Korean Physical Therapy
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• v.14 no.2
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• pp.16-25
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• 2002

Low energy laser irradiation(LELI) therapy in physical therapy is widespread but the mechanisms are not fully understood. The purpose of the present study was to examine the epidermal growth factor(EGF)'s expression within lumbar spinal cord which corresponding with crushed extensor digitorum longus(EDL) of rats after low-power laser irradiation applied. After a crushed injury on the right EDL, low-power laser irradiation was applied by using 2000mW, 2000Hz, 830nm GaAlAs(Gallium-aluminum-arsenide) semiconductor diode laser. The laser treatment was performed with 10 minutes daily for 3days. After EDL crush injury, EGF immunoreactive positive neurons in experimental group were progressively decreased from the first to third days. Especially 1 day subgroup is highly expressed in dorsal horn(Lamina I, II, III) and around of central cannal of spinal cord(Lamina VII). Control group was only expressed slightly at 3 days. This study suggests that LELI stimulate that release and migration of EGF in spinal cord, which distict to wound site, therfore promote wound healing of EDL crush injury.

• Journal of the Korean Applied Science and Technology
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• v.38 no.3
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• pp.750-761
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• 2021

This study was performed to observe the responses of angiogenesis-related protein expression in skeletal muscle of aged rats by regular resistance exercise training with aerobic exercise. For the purpose of the study, naturally aged SD rats (20-24 months, N=18) were used and divided into control (CON, n=6), resistance exercise (RE, n=6), and resistance + aerobic exercise (RE + AE, n=6) groups. RE group performed 3 sets × 4 exercises each session using a ladder for laboratory animals, and RE +AE group performed 2 sets × 3 times of ladder climbing and additional treadmill running (30 min) each session. After 8 weeks of exercise training, soleus muscle and extensor digitorum longus muscle (EDL) were extracted and used for analysis. Western blot was performed to analyze the expression levels of angiogenesis-related proteins (HIF-1α, VEGF, FLK-1, Ang-1, Ang-2) in skeletal muscle. As a result of the study, the expression of HIF-1α, VEGF, FLK-1, Ang-1, and Ang-2 proteins in soleus muscle (type I muscle) was higher in RE +AE than in CON group, and HIF-1α, VEGF, Ang-1, Ang-2 protein expression of RE group was higher than that of CON group. Furthermore, Ang-2 to Ang-1 ratio of RE + AE group was higher than that of RE group, showing differences by exercise type. In EDL muscle (type II muscle), HIF-1α was increased only by RE group, whereas VEGF and FLK-1 protein expressions were increased in both training types, and no difference was observed between the types of exercise training. In addition, there was no difference in angiopoieitin protein expressions in EDL muscle by exercise training. Therefore, in aging, regular exercise training induces skeletal muscle angiogenic response regardless of exercise type, and in particular, the combination of aerobic exercise with resistance exercise may have an additional positive effect on angiogenesis in type I muscle.