• Bulletin of the Korean Chemical Society
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• v.31 no.9
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• pp.2607-2612
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• 2010

Human tissue-type plasminogen activator (tPA) is a valuable thrombolytic agent used to successfully treat acute myocardial infarction, thromboembolic stroke, peripheral arterial occlusion, and venous thromboembolism. Recombinant tPA is accumulated as an inactive form in inclusion bodies of E. coli and is refolded in vitro, which is accompanied by extensive aggregation. In the present study, a tPA protease domain was expressed in an active soluble form in the cytosol of E. coli Rosetta-gami cells, which allowed disulfide bond formation and supplied the tRNA molecules required for six rarely used codons in E. coli. This strategy increased the amount of soluble protease domain protein and avoided the cumbersome refolding process. The purified protease domain not only degraded tPA substrate peptides but also formed a covalently bound complex with plasminogen activator inhibitor-1, as does full-length tPA. Soluble expression and purification of tPA domains may aid in functional analyses of this multi-domain protein, which has been implicated in many physiological and pathological processes.

• Proceedings of the KIEE Conference
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• 1997.11a
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• pp.77-79
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• 1997

This paper describes a nonlinear state observer to identify the rotor position and velocity of a hybrid-type linear pulse motor(LPM). Since the observer's error dynamics of a hybrid-type LPM is highly nonlinear, the stability of the proposed observer is analyzed using an approximated error model. The optimal choices of the proposed observer gains are also discussed. Our extensive simulation study shows that the proposed observer can identify rotor position and velocity and is robust with respect to uncertainties in mechanical parameters and load force.

• Steel and Composite Structures
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• v.32 no.1
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• pp.111-126
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• 2019

This paper deals with experimental investigation and modeling of the static behavior of a novel RCS beam-column exterior joint. The studied joint detail is a through-column type in which an H steel profile totally embedded inside RC column is directly welded to the steel beam. The H steel profile was covered by two supplementary plates in the joint area in order to avoid the stirrups resisting shear in the joint area. Two full-scale through-column-type RCS joints were tested under static loading. The objectives of the tests were to examine the connection performance and to highlight the contribution of two supplementary plates on the shear resistance of the joint. A reliable nonlinear 3D finite element model was developed using ABAQUS software to predict the response and behavior of the studied RCS joint. An extensive parametric study was performed to investigate the influences of the stirrups, the encased profile length and supplementary plate length on the behavior of the studied RCS joint.

• Journal of mucopolysaccharidosis and rare diseases
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• v.5 no.1
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• pp.22-28
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• 2021

Mucopolysaccharidosis type III (MPS III) or Sanfilippo disease is an orphan-inherited lysosomal storage disease. It is one of the most common MPS subtypes. The classical presentation is an infantile-onset neurodegenerative disease characterized by intellectual regression, behavioral and sleep disturbances, loss of ambulation, and early death. Unlike other MPS, no disease-modifying therapy has been approved. Here, we review the curative therapy developed for MPS III, from historically ineffective hematopoietic stem cell transplantation and substrate reduction therapy to the promising enzyme replacement therapy or adeno-associated/lentiviral vector-mediated gene therapy. Preclinical studies are presented with recent translational first-in-man trials. We also present experimental research with preclinical mRNA and gene-editing strategies. Lessons from animal studies and clinical trials have highlighted the importance of early therapy before extensive neuronal loss. Disease-modifying therapy for MPS III will likely mandate the development of new early diagnosis strategies.

• Archives of Craniofacial Surgery
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• v.21 no.5
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• pp.276-282
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• 2020

Background: Orbital fractures are the most common pediatric facial fractures. Treatment is conservative due to the anatomical differences that make children more resilient to severe displacement or orbital volume change than adults. Although rarely, extensive fractures may result in enophthalmos, causing cosmetic problems. We aimed to establish criteria for extensive fractures that may result in enophthalmos. Methods: We retrospectively reviewed the charts of patients aged 0-15 years diagnosed with orbital fractures in our hospital from January 2010 to February 2019. Computed tomography images were used to classify the fractures into linear, trapdoor, and open-door types, and to estimate the defect size. Data on enophthalmos severity (Hertel exophthalmometry results) and fracture pattern and size at the time of injury were obtained from patients who did not undergo surgery during the follow-up and were used to identify the surgical indications for pediatric orbital fractures. Results: A total of 305 pediatric patients with pure orbital fractures were included-257 males (84.3%), 48 females (15.7%); mean age, 12.01±2.99 years. The defect size (p=0.002) and fracture type (p=0.017) were identified as the variables affecting the enophthalmometric difference between the eyes of non-operated patients. In the linear regression analysis, the variable affecting the fracture size was open-door type fracture (p<0.001). Pearson's correlation analysis demonstrated a positive correlation between the enophthalmometric difference and the bony defect size (p=0.003). Using receiver operating characteristic curve analysis, a cutoff value of 1.81 ㎠ was obtained (sensitivity, 0.543; specificity, 0.724; p=0.002). Conclusion: The incidence of enophthalmos in pediatric pure orbital fractures was found to increase with fracture size, with an even higher incidence when open-door type fracture was a cofactor. In clinical settings, pediatric orbital fractures larger than 1.81 ㎠ may be considered as extensive fractures that can result in enophthalmos and consequent cosmetic problems.

• Atmosphere
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• v.16 no.3
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• pp.235-246
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• 2006

Remote sensing techniques using satellites or the scanning weather radars depend mostly on the presence of clouds or precipitation, and leave the extensive regions of clear air unobserved. But wind profilers provide the most direct measurements of mesoscale vertical air motion in the troposphere, even in the context of heavy precipitation. In this paper, the precipitation events during the Changma period was classified into 4 precipitation types - stratiform, mixed stratiform/ convective, deep convective, and shallow convective. The parameters for the classification of analysis are the vertical structure of reflectivity, Doppler velocity, and spectral width measured with the wind profiler at Haenam for a three-year period (2003-2005). In addition, the synoptic fields and total amount of precipitation were analyzed using the Global Final Analyses (FNL) data and the Global Precipitation Climatology Project (GPCP) data. During the Changma period, the results show that the stratiform type was dominant under the moist-neutral atmosphere in 2003, whereas the deep convective type was under the moist unstable condition in 2004. The stratiform type was no less popular than the deep convective type among four seasons because the moist neutral layer was formed by the convergence between the upper-level jet and the low-level jet, and by the moisture transport along the western rim of the North Pacific subtropical anticyclone.

• Proceedings of the IEEK Conference
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• 1998.10a
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• pp.1191-1194
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• 1998

This paper presents a new computationally efficient adaptive algorithm for blind signal separation, which is able to recover the narrowband source signals in the presence of cochannel interference without a prior knowledge of array manifold. We derive a new blind signal separation algorithm using the Natural gradient 〔1〕from an information-theoretic approach. The resulting algorithm has the Bussgang property which has been widely used in blind equalization 〔12〕. Extensive computer simulation results comfirm the validity and high performance of the proposed algorithm.

• Proceedings of the Korea Concrete Institute Conference
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• 2000.10b
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• pp.1025-1030
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• 2000

Recently, surface-treatment agent was developed to restore performance of the deteriorated concrete and inhibiting corrosion of the reinforcing-bar. The performance of surface-treatment agent was verified and this agent has been broadly in America and European countries. However, this type agent has not been used broadly in Korea because performance of the agent isn't verify by a proper test method yet. In this study, we suggested our own test procedures and methods through extensive laboratory tests to verify the performance of concrete after applying the surface-treatment agent.

• The Journal of Korea Robotics Society
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• v.1 no.1
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• pp.1-8
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• 2006

In this paper, we propose a methodology for classifying types of lower limb disability and their mechanical structure, based on extensive survey of previous developments. We also propose a task-oriented design with human-friendly and energy-efficient assistive system. The result can be used for optimal design of wearable walking-assistive robot considering the type of disability and the content of task.

• Journal of Korean Academy of Nursing
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• v.41 no.4
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• pp.491-499
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• 2011

Purpose: The study was conducted to develop and test a hypothetical model which explains self-care behavior in patients with type 2 diabetes was established based on the Self-Determination Theory. Methods: The participants were 218 patients with type 2 diabetes mellitus enrolled in an outpatient clinic of one endocrine center in Korea. The data were collected using questionnaires from April 5 through May 7, 2010. The descriptive and correlation statistics were analyzed using the SPSS/WIN 15.0 and the structural equation modeling procedure was performed using the AMOS 7.0 program. Results: The results of this study showed that competence and autonomous motivation were the strong factors influencing self-care behavior in patients in this sample. Support from health provider for autonomy was a significant indirect factor on self-care behavior. These factors explained 64.9% of variance in the participants' self care behavior. The proposed model was concise and extensive in predicting self-care behavior of the participants. Conclusion: Findings may provide useful assistance in developing effective nursing interventions for maintaining and promoting self-care behavior in patients with type 2 diabetes.