• The Journal of Korean Society of Virology
• /
• v.29 no.4
• /
• pp.221-233
• /
• 1999

Prospect Hill Virus (PHV) is the well known serotype of hantavirus, a newly established genus in family Bunyaviridae. Extensive studies have upheld the original view of PHV genetics with three genes such as nucleocapsid (N) protein, envelope proteins (G1, G2) and RNA dependent RNA polymerase. In this study, we report the existence of additional gene that is encoded in an overlapping reading frame of the N protein gene within S genome segment of PHV. This gene is expected to encode a nonstructural small (NSs) protein and it seems to be only found in PHV infected cell. The presence and synthesis of NSs protein could be demonstrated in the cell infected with PHV using anti-peptide sera specific to the predicted amino acid sequence deduced from the second open reading frame. Ribosomal synthesis of this protein appears to occur at AUG codon at the 83rd base of S genome segment, downstream of N protein initiation codon. This protein is small in size (10.4 KDa) and highly basic in nature. The expression strategy of NSs protein appears that a signal mRNA is used to translate both N and NSs protein in PHV infected cell. 10 KDa protein in virus infected cell lysates can bind to mimic dsRNA. This fact strongly suggests that NSs protein may be involved in virus replication on late phase of viral life cycle.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.11 no.4
• /
• pp.337-343
• /
• 2006

Trimethylamine dehydrogenase (TMADH, EC 1.5.99.7), an iron-sulfur flavoprotein that catalyzes the oxidative demethylation of trimethylamine to form dimethylamine and formaldehyde, was purified from Methylophaga sp. strain SK1. The active TMADH was purified 12.3-fold through three purification steps. The optimal pH and temperature for enzyme activity was determined to be 8.5 and $55^{\circ}C$, respectively. The $V_{max}\;and\;K_m$ values were 7.9 nmol/min/mg protein and 1.5 mM. A genomic DNA of 2,983 bp from Methylophaga sp. strain SK1 was cloned, and DNA sequencing revealed the open reading frame (ORF) of the gene coding for TMADH. The ORF contained 728 amino acids with extensive identity (82%) to that of Methylophilus methylotrophus $W_3A_1$.

• International Journal of Advanced Culture Technology
• /
• v.9 no.4
• /
• pp.345-354
• /
• 2021

Data quality issue in information systems is analyzed with focus on conceptual data modeling. Extensive investigation through triangulation of case studies is attempted to find how much extent inappropriate data modeling practices exercised in real workplace environment. It is revealed that more than 40 percent of data adversely contributed to unnecessary data redundancy, i.e., the level of data obesity is over 40 percent. Another contribution of this paper lies in excavation of all the categories of inappropriate data modeling practices, which has been previously only partially uncovered in the literature. New findings in this paper prove that the extent of inappropriate modeling is more serious that previously reported.

• The Journal of the Convergence on Culture Technology
• /
• v.6 no.4
• /
• pp.225-230
• /
• 2020

This paper highlights the influence and the importance of the syntactic-grammatical knowledge on "the reading machine", appeared in Jackendoff (1999). Due to the lack of the detailed testing and implementation in his research, this paper tests an extensive data array using a component of Google Translate, currently available freely and most widely on the internet. Although outdated, Jackendoff's paper, "Why can't Computers use English?", argues that syntactic-grammatical knowledge plays a key role in the outputs of computers and computer-based reading machines. The current research has implemented some testings of his thought-provoking examples, in order to find out whether Google Translate can handle the same problems after two decades or so. As a result, it is argued that in the field of NLP, I-language in the sense of Chomsky (1986, 1995 etc) is real and the syntactic, grammatical, and categorial knowledge is essential in the faculty of language. Therefore, it is reassured in this paper that when it comes to human language, even the most advanced "machine" is still no match for human faculty of language, the syntactic-grammatical knowledge.

• Architectural research
• /
• v.20 no.2
• /
• pp.35-43
• /
• 2018

This article explores the complex modes of experiencing the modern city that are engaging and disengaging by nature, which thus negates any simple ways of understanding what it means by 'the urban' in a Manichean comparison. What follows is an in-depth case study of Gordon Matta-Clark's 1976 film titled City Slivers. Influenced by the countercultural practices prevalent in the 1960s and 1970s, Matta-Clark produced a number of works roughly grouped together under the rubric of "building cuts." Among many others, City Slivers is distinctive among Matta-Clark's extensive cutting projects, in the sense that he actively utilizes film as a primary expressive medium and poetically reassembles fragmentary images of cityscape in order to bring forth an alternative urban scenario where the tension between institution-bound urbanization and dispersed daily urban practices is highlighted. Instead of simply being critical against the changing urban conditions of Manhattan in the 1970s, Matta-Clark aims to actively grasp ambivalent instances of urban life that are at once attractive and alienating, thereby excavating the subconscious terrain of contemporary urbanism that is prevalent but often dismissed over glamorous urban projects.

• Journal of Microbiology and Biotechnology
• /
• v.13 no.5
• /
• pp.783-788
• /
• 2003

A 27 bp $tRNA^{Gly}$ region (att1) was identified as the integration site for a 12,384 bp Pfl-derived genomic island containing 15 open reading frames (ORFs) from PA0715 to PA0729 in P. aeruginosa strain PAOl. Homologous island was observed in P. aeruginosa strain PA14, but not in P. aeruginosa strain K (PAK). We isolated the Pfl island from PA14, and determined its 10,657 bp sequences containing 14 ORFs, with significant sequence variations near the borders. In contrast to the PAO1 Pfl island, the PA14 Pfl island was integrated at the 10 bp att2 site between PA1191 and PA1192. The attl site of PA14, however, was still occupied by a third genetic segment, whereas both attl and att2 sites of PAK remained unutilized. These results exemplify an extensive genomic variation of Pfl-related islands involving differential genetic organizations and differential att site utilizations.

• Journal of the Korean Wood Science and Technology
• /
• v.46 no.4
• /
• pp.415-422
• /
• 2018

Terpenoids have a wide range of biological functions and have extensive applications in the pharmaceutical, cosmetic, and flavoring industry. The white-rot fungus, Polyporus brumalis, is able to synthesize terpenoids via terpene synthase, which catalyzes an important step that forms a large variety of sesquiterpene products from farnesyl pyrophosphate (FPP). To improve the production of sesquiterpenes, the terpene synthase gene was isolated from Polyporus brumalis and was heterologously transformed into a Pichia pastoris strain. The open reading frame of the isolated gene (approximately 1.2 kb) was inserted into Pichia pastoris to obtain a recombinant enzyme. Five transformants were obtained and the expression of terpene synthase was analyzed at the transcript level by reverse transcription PCR (polymerase chain reaction) and at the protein level by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Expression of the terpene synthase gene product was elevated in the transformants and as expected the molecular weight of the protein was approximately 45 kDa. These recombinant enzymes have potential practical applications and future studies should focus on their functional characterization.

• Korean Journal of Child Studies
• /
• v.13 no.2
• /
• pp.203-216
• /
• 1992

The purpose of this study was to investigate the utility of the Luria-Nebraska Battery-Children's Revision (LNNB-CR) in the clinical situation in Korea: LNNB-CR was applied clinically in order to examine how well it differentiates brain damaged children from normals. Subjects were 30 children with the average age of 10 years. Among them 15 were diagnosed as the 'brain damaged' group, and the remaining 15 were normal. All subjects of the two groups were tested on all items in accordance with LNNB-CR manual. Data were analyzed by percentage, correlation coefficient, and t-test. The results were (1) the degree of consistency between testers averaged 97.2％ which confirmed the stability of the scoring system. (2) Split-half reliability was ranged from .75 on the Tactile Scale (C3) to .95 on the Memory Scale (C10). Thus, consistency of items within the scales appeared high. (3) Internal consistency reliability ranged from .74 on the Visual Scale (C4) to .98 on the Reading Scale (C9). Thus, the homogenity of items within the scales appeared high. (4) In the diagnostic discriminative power test between the two groups, both individual scales and total scores showed significant differences at the level of p<.001. (5) The discriminative power test between two groups on all items showed significant differences at the level of p<.05 or better in 126(85％) out of 149 items. These results are supportive of the diagnostic utility of the application of LNNB-CR to the clinical situation in Korea. However, extensive additional research is needed in order to prove its worth.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.35 no.7
• /
• pp.761-774
• /
• 2011

This study investigated the consumer review information considered important by consumers when making a purchase decision to buy apparel products online. Data were collected through focus group interviews. Eleven females in their 20s and 30s, who have extensive experience in reading consumer reviews posted on online apparel stores, participated in the study. The consumer review information considered important by participants is the information related to seven product attributes (size, fabric, design, color, sewing, price, and country of origin), seven benefits (functional, financial, esthetic, emotional, social, utilitarian benefits, and product value compared to price) of the apparel product and four store attributes (return/refund, delivery, reputation/credibility, and customer service). The findings from the study can serve as an important tool in developing survey questions in order to evaluate the quality of consumer review information and help online retailers plan methods to improve the quality of reviews.

• Journal of fish pathology
• /
• v.26 no.3
• /
• pp.295-301
• /
• 2013

Ferritin is an evolutionarily conserved protein that plays an important role in iron storage and detoxification. In this study, the gene encoding a ferritin H subunit homologue (RbFH) was cloned from rock bream (Oplegnathus fasciatus) and analyzed at the expression. The full-length ferritin H cDNA was 1162 bp long and contained an open reading frame (ORF) of 531 bp that encoded 177 amino acid residues with a predicted molecular mass of 20.8 kDa. The 5' UTR was 297 bp in length, and the 3' UTR 298 bp, and preceded by a 5'-untranslated region that contains a putative Iron Regulatory Element (IRE). The deduced amino acid sequence of RbFH shares extensive sequence identities with the H ferritins of a number of fish species and contains the ferroxidase center that is preserved in ferritin H subunits. Examination of tissue specific expression indicated that RbFH expression was most abundant in PBLs, RBC, liver and muscle.